公开(公告)号：US4128470A

公开(公告)日：1978-12-05

申请号：US879421

申请日：1978-02-21

申请人： Nobuo Hiratsuka ,  Nakatsugu Yaginuma

发明人： Nobuo Hiratsuka ,  Nakatsugu Yaginuma

IPC分类号： G01N27/447 ,  G01N27/26 ,  G01N27/28

CPC分类号： G01N27/44795 ,  G01N27/44747

摘要： A support for electrophoresis comprising a porous polymer film having an electroendosmosis factor of 0 to plus about 10 mm, and a process for the production of the support comprising adding a nonionic surface active agent and/or cellulose ether to a mixed solution comprising at least a polymer material for formation of a film and a solvent to produce a porous polymer film.

摘要翻译： 一种用于电泳的载体，其包括具有0至+ 10mm的电内渗因子的多孔聚合物膜，以及用于制备所述载体的方法，其包括将非离子表面活性剂和/或纤维素醚加入到至少包含 用于形成膜和溶剂的聚合物材料以产生多孔聚合物膜。

公开(公告)号：US4006069A

公开(公告)日：1977-02-01

申请号：US632884

申请日：1975-11-17

申请人： Nobuo Hiratsuka ,  Nakatsugu Yaginuma

发明人： Nobuo Hiratsuka ,  Nakatsugu Yaginuma

IPC分类号： G01N27/26 ,  B01D69/10 ,  G01N27/447 ,  G01N30/92 ,  G01N27/28

CPC分类号： G01N27/44747 ,  B01D69/10

摘要： A support for use in electrophoretic analysis comprising a porous polymeric flat plate such as filter paper, cellulose acetate or a non-woven fabric and a polymeric gel, such as polyacrylamide gel, enclosed in the open cells of the flat plate. The gel membrane is tough and does not shrink upon drying. The support can also be used as a filter.

摘要翻译： 用于电泳分析的载体，包括封闭在平板的开孔中的多孔聚合物平板如滤纸，乙酸纤维素或无纺织物以及聚合物凝胶如聚丙烯酰胺凝胶。 凝胶膜是坚韧的，干燥后不会收缩。 支撑也可以用作过滤器。

公开(公告)号：US4171987A

公开(公告)日：1979-10-23

申请号：US558099

申请日：1975-03-13

申请人： Masaru Horiguchi ,  Nobuo Hiratsuka ,  Sumio Otani

发明人： Masaru Horiguchi ,  Nobuo Hiratsuka ,  Sumio Otani

IPC分类号： B01D39/18 ,  C08J9/28 ,  C08L1/10 ,  C08L1/12 ,  G01N27/447

CPC分类号： B01D71/16 ,  B01D69/02 ,  B01D71/18 ,  B01D71/20 ,  B01D71/22 ,  C08L1/10 ,  G01N27/44747

摘要： A micro-porous sheet having excellent thermostability which contains a cellulose ester as a basic component and 0.1 to 20 wt% of a cellulose ether based on the cellulose ester.

摘要翻译： 一种具有优异的热稳定性的微孔片，其含有纤维素酯作为碱性成分，0.1〜20重量％的纤维素醚基于纤维素酯。

公开(公告)号：US4337065A

公开(公告)日：1982-06-29

申请号：US191784

申请日：1980-09-29

申请人： Nobuo Hiratsuka ,  Yuji Mihara ,  Nobuhito Masuda ,  Takushi Miyazako

发明人： Nobuo Hiratsuka ,  Yuji Mihara ,  Nobuhito Masuda ,  Takushi Miyazako

IPC分类号： G03C1/00 ,  G01N33/536 ,  G01N33/543 ,  G01N33/54 ,  G01N33/52 ,  G01N33/58

CPC分类号： G01N33/54386

摘要： In a photochemical immunological assay, an antigen or antibody is labelled with a spectral sensitizer or fogging agent for photographic use, and then an immune reaction is caused. The resulting reaction mixture is dropwise added to a multilayer analysis sheet. After either one of the labelled antigen or antibody (F) or the labelled antigen-antibody bound product (B) is brought into contact with silver halide by passing either one through a B/F separating layer provided in the multilayer analysis sheet, the analysis sheet is exposed with a light that the spectral sensitizer or fogging agent absorbs. Then, the resulting optical density obtained by development processing when a spectral sensitizer is used, or without development processing when a fogging agent is used is measured.The multilayer analysis sheet comprises a support having provided thereon a silver halide layer and a B/F separating layer and makes the photochemical immunological assay simpler and more accurate.

摘要翻译： 在光化学免疫测定中，抗原或抗体用光谱增感剂或雾化剂标记，用于照相用途，然后引起免疫反应。 将所得反应混合物滴加到多层分析片中。 将标记的抗原或抗体（F）或标记的抗原 - 抗体结合产物（B）中的任一个通过使任一种通过多层分析片中提供的B / F分离层与卤化银接触，分析 片材被光谱增感剂或起雾剂吸收的光暴露。 然后，测量当使用光谱增感剂时通过显影处理获得的所得光密度，或者当使用起雾剂时不进行显影处理。 多层分析片包括其上设置有卤化银层和B / F分离层的载体，并使光化学免疫测定更简单并且更准确。

公开(公告)号：US4483928A

公开(公告)日：1984-11-20

申请号：US415300

申请日：1982-09-07

申请人： Tatsuo Suzuta ,  Nobuo Hiratsuka

发明人： Tatsuo Suzuta ,  Nobuo Hiratsuka

IPC分类号： G01N33/53 ,  A61K39/395 ,  G01N33/543 ,  G01N33/544 ,  G01N33/569 ,  G01N33/54

CPC分类号： G01N33/5432 ,  G01N33/54313 ,  G01N33/56972 ,  Y10S436/829

摘要： Microcapsules are sensitized with antibody to antigen of lymphocyte. Using such microcapsules, B cells and/or T cells can be selectively measured separately or simultaneously, in a simple manner, based on cell-mediated immunity which comprises applying the microcapsules sensitized with antibody to antigen of lymphocyte and sheep red cells or another kind of antibody-sensitized microcapsules that are optically distinguishable from the first antibody-sensitized microcapsules, to lymphocyte at the same time and thereafter independently measuring respective cells that are optically distinguishable from each other.The operation for the measurement is extremely simple and neither skill nor special equipment is required.

摘要翻译： 微胶囊对淋巴细胞抗原的抗体敏感。 使用这样的微胶囊，B细胞和/或T细胞可以单独或同时以简单的方式基于细胞介导的免疫力来选择性地测量，其包括将用抗体敏化的微胶囊施用于淋巴细胞和绵羊红细胞的抗原或另一种 与第一抗体敏化微胶囊光学上可区分的抗体敏化微胶囊同时和淋巴细胞，然后独立地测量彼此光学上可区分的各个细胞。 测量操作非常简单，不需要技能和特殊设备。

公开(公告)号：US4337063A

公开(公告)日：1982-06-29

申请号：US126920

申请日：1980-03-03

申请人： Yuji Mihara ,  Nobuhito Masuda ,  Nobuo Hiratsuka ,  Takushi, both of Miyazako ,  Tadashi Ikeda

发明人： Yuji Mihara ,  Nobuhito Masuda ,  Nobuo Hiratsuka ,  Takushi, both of Miyazako ,  Tadashi Ikeda

IPC分类号： G01N33/536 ,  G01N33/532 ,  G01N33/537 ,  G01N33/54 ,  G01N33/58

CPC分类号： G01N33/537 ,  G01N33/532 ,  Y10S436/805 ,  Y10S436/815 ,  Y10S436/817

摘要： Method for immunological analysis of a trace component(s) by labelling an antigen or antibody with a marker comprising causing an immune reaction using an antigen or antibody labelled with a spectral sensitizer as a marker, separating the labelled antigen or antibody from the resulting antigen-antibody reaction product, then bringing either the labelled antigen or antibody and the labelled antigen-antibody reaction product into contact with a silver halide, exposing the resulting system to light having a wavelength which the spectral sensitizer absorbs, developing the thus exposed silver halide and then measuring the resulting density.Trace components can thus be photochemically detected and the amount present quantitatively determined. The method provides high reproducibility and high sensitivity comparable to radioimmunoassay but without any risk of radiation exposure.

摘要翻译： 通过使用标记抗原或抗体来标记抗原或抗体的免疫学分析方法，所述标记包括使用用光谱增感剂标记的抗原或抗体作为免疫反应作为标记，从得到的抗原 - 然后使标记的抗原或抗体和标记的抗原 - 抗体反应产物与卤化银接触，将所得体系暴露于具有光谱增感剂吸收的波长的光，从而显现出由此暴露的卤化银，然后 测量所得密度。 因此可以光化学检测痕量成分，定量确定量。 该方法提供了与放射免疫测定相当的高重现性和高灵敏度，但没有任何辐射暴露的风险。

公开(公告)号：US5019347A

公开(公告)日：1991-05-28

申请号：US481800

申请日：1990-02-20

申请人： Nobuo Hiratsuka ,  Asaji Kondo

发明人： Nobuo Hiratsuka ,  Asaji Kondo

IPC分类号： G01N31/22 ,  G01N33/52

CPC分类号： G01N33/525 ,  Y10S435/805

摘要： In an integral multilayer analytical element of a flat sheet type comprising a plurality of layers for quantitative analysis of an analyte in a liquid sample, the improvement which comprises at least two layers of a microporous sheet material arranged adjacent to each other, said at least two layers being combined by an adhesive provided in part on the interface of said layers, whereby the interface allows essentially uniform passage of a liquid therethrough.

摘要翻译： 在包括用于液体样品中的分析物的定量分析的多个层的平板型的整体多层分析元件中，所述改进包括彼此相邻布置的至少两层微孔片材料，所述至少两层 层通过部分地设置在所述层的界面上的粘合剂组合，由此界面允许液体基本上均匀地通过其中。

公开(公告)号：US4342739A

公开(公告)日：1982-08-03

申请号：US110318

申请日：1980-01-08

申请人： Fujio Kakimi ,  Nobuo Hiratsuka ,  Hiroharu Matsukawa

发明人： Fujio Kakimi ,  Nobuo Hiratsuka ,  Hiroharu Matsukawa

IPC分类号： G01N33/543 ,  G01N33/545 ,  G01N33/58 ,  B01J13/02 ,  G01N33/60

CPC分类号： G01N33/54313 ,  G01N33/5434 ,  G01N33/545 ,  G01N2446/20 ,  G01N2446/30 ,  G01N2446/90 ,  Y10S436/823 ,  Y10S436/829 ,  Y10T428/2984 ,  Y10T428/2985

摘要： Antigen- or antibody-bound microcapsules are employed for immunological assay of biochemical components having immunological activity. The microcapsule system provides high detection sensitivity, high accuracy and excellent reproducibility in immunological assay, without causing non-specific reaction. The microcapsules can be labelled with isotopes, enzymes, etc., for use in quantitative assays of trace amounts of biochemical components.

摘要翻译： 抗原或抗体结合的微胶囊用于具有免疫活性的生物化学成分的免疫测定。 微胶囊系统在免疫测定中提供高检测灵敏度，高精度和优异的再现性，而不引起非特异性反应。 微胶囊可以用同位素，酶等标记，用于微量生化成分的定量测定。

公开(公告)号：US5068019A

公开(公告)日：1991-11-26

申请号：US525963

申请日：1990-05-18

申请人： Takashi Yoshida ,  Tsueno Kawase ,  Nobuo Hiratsuka

发明人： Takashi Yoshida ,  Tsueno Kawase ,  Nobuo Hiratsuka

IPC分类号： G01N27/447

CPC分类号： G01N27/44747

摘要： An electrophoresis film support particularly useful for the electrophoretic separation of serum proteins. According to one aspect, the electrophoresis film support comprises a microporous polymer membrane sheet containing therein an additive which is selected from the group consisting of hydroxypropyl cellulose, triphenyl phosphate, tricresyl phosphate and mixtures thereof. According to another aspect the electrophoresis film support comprises a microporous membrane sheet made of a polymer material containing therein an additive which is selected from the group consisting of higher fatty acids having 10 to 27 carbon atoms and mixtures thereof, the additive being contained in an amount, based on the weight of the polymer material, ranging from 10 ppm to 3,000 ppm or the maximum solubility limit of the higher fatty acids in the polymer material.

公开(公告)号：US4868131A

公开(公告)日：1989-09-19

申请号：US72499

申请日：1987-07-13

申请人： Nobuo Hiratsuka

发明人： Nobuo Hiratsuka

IPC分类号： G01N33/536 ,  G01N33/538 ,  G01N33/543

CPC分类号： G01N33/538 ,  G01N33/54313 ,  G01N33/54386

摘要： An immunoassay process for quantitative analysis of an analyte antigen (or analyte antibody) in an aqueous liquid sample. In the first step, the analyte antigen (or analyte antibody) is allowed to react with an antibody (or antigen) in competition with a known quantity of a labelled antigen (or labelled antibody), the antibody (or antigen) being carried by water-insoluble microparticles in the immobilized state. In the second step, the reaction mixture is spotted on a top porous layer of an integral multi-layered analysis element which includes the top porous layer, an intermediate detection layer and a bottom water-impermeable and transparent support. The top porous layer permeates aqueous solution and has pores sufficiently small to trap all of the microparticles carrying the antibodies (or antigens) bound to the analyte antigen (or analyte antibody) and bound to the labelled antigen (or labelled antibody). Thus, the free labelled antigen is allowed to pass through the top porous layer and migrate to the detection layer. In the third step, the quantity of the marker labelling to the labelled antigen (or labelled antibody) which has not been bound to the immobilized antibody (immobilized antigen) is determined.

摘要翻译： 用于定量分析含水液体样品中的分析物抗原（或分析物抗体）的免疫测定方法。 在第一步中，使分析物抗原（或分析物抗体）与已知量的标记抗原（或标记的抗体）竞争的抗体（或抗原）反应，抗体（或抗原）由水携带 不溶性微粒处于固定状态。 在第二步骤中，将反应混合物点在包括顶部多孔层，中间检测层和底部不透水透明载体的整体多层分析元件的顶部多孔层上。 顶部多孔层渗透水溶液并且具有足够小的孔以捕获携带与分析物抗原（或分析物抗体）结合并结合到标记抗原（或标记抗体）的抗体（或抗原）的所有微粒。 因此，允许游离标记的抗原通过顶部多孔层并迁移到检测层。 在第三步骤中，确定标记为未结合固定化抗体（固定化抗原）的标记抗原（或标记抗体）的标记物的量。