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    .alpha.-amylase mutants
    3.
    发明授权
    .alpha.-amylase mutants 失效
    α-淀粉酶突变体

    公开(公告)号：US6022724A

    公开(公告)日：2000-02-08

    申请号：US683838

    申请日：1996-07-18

    申请人： Allan Svendsen ,  Henrik Bisg.ang.rd-Frantzen ,  Torben Borchert

    发明人： Allan Svendsen ,  Henrik Bisg.ang.rd-Frantzen ,  Torben Borchert

    IPC分类号： C12N15/09 ,  C11D3/386 ,  C12N1/21 ,  C12N9/28 ,  C12N15/00 ,  C12N15/56 ,  C12R1/07 ,  C11D3/00 ,  C12N9/30

    CPC分类号： C12N9/2417 ,  C11D3/386

    摘要： The present invention relates to a method of constructing a variant of a parent Termamyl-like .alpha.-amylase, which variant has .alpha.-amylase activity and at least one altered property as compared to the parent .alpha.-amylase, comprisesi) analyzing the structure of the parent Termamyl-like .alpha.-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like .alpha.-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like .alpha.-amylase (as evaluated on the basis of structural or functional considerations),ii) constructing a Termamyl-like .alpha.-amylase variant, which as compared to the parent Termamyl-like .alpha.-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and, optionally,iii) testing the resulting Termamyl-like .alpha.-amylase variant with respect to the property in question.

    摘要翻译： 本发明涉及构建母体类戊酰样α-淀粉酶的变体的方法，该变体与亲本α-淀粉酶相比具有α-淀粉酶活性和至少一种改变的性质，包括：i）分析 鉴定至少一个氨基酸残基或类甲酰样α-淀粉酶结构的至少一个结构部分，所述氨基酸残基或结构部分被认为与改变其性质有关 母体类戊酰样α-淀粉酶（根据结构或功能考虑进行评估），ii）构建与母体类戊酰样α-淀粉酶相比的类戊酰样α-淀粉酶变体，已经在 在i）中鉴定的氨基酸残基或结构部分，以改变其性质，以及任选地，iii）相对于所讨论的性质测试所得到的类戊酰样α-淀粉酶变体。

    .alpha.-amylase mutants
    4.
    发明授权
    .alpha.-amylase mutants 失效
    α-淀粉酶突变体

    公开(公告)号：US5989169A

    公开(公告)日：1999-11-23

    申请号：US600908

    申请日：1996-02-13

    申请人： Allan Svendsen ,  Henrik Bisg.ang.rd-Frantzen ,  Torben Vedel Borchert

    发明人： Allan Svendsen ,  Henrik Bisg.ang.rd-Frantzen ,  Torben Vedel Borchert

    IPC分类号： C12N15/09 ,  C11D3/386 ,  C12N1/21 ,  C12N9/28 ,  C12N15/00 ,  C12N15/56 ,  C12R1/07 ,  C12N9/26 ,  C07H21/04

    CPC分类号： C12N9/2417 ,  C11D3/386

    摘要： The present invention relates to a method of constructing a variant of a parent Termamyl-like .alpha.-amylase, which variant has .alpha.-amylase activity and at least one altered property as compared to the parent .alpha.-amylase, comprising i) analyzing the structure of the parent Termamyl-like .alpha.-amylase to identify at least one amino acid residue or at least one structural part of the Termamyl-like .alpha.-amylase structure, which amino acid residue or structural part is believed to be of relevance for altering the property of the parent Termamyl-like .alpha.-amylase (as evaluated on the basis of structural or functional considerations), ii) constructing a Termamyl-like .alpha.-amylase variant, which as compared to the parent Termamyl-like .alpha.-amylase, has been modified in the amino acid residue or structural part identified in i) so as to alter the property, and iii) testing the resulting Termamyl-like .alpha.-amylase variant for the property in question.

    摘要翻译： 本发明涉及一种构建母体类戊酰样α-淀粉酶的变体的方法，该变体与亲本α-淀粉酶相比具有α-淀粉酶活性和至少一种改变的性质，其包括：i）分析 鉴定至少一个氨基酸残基或类甲酰样α-淀粉酶结构的至少一个结构部分，所述氨基酸残基或结构部分被认为与改变其性质有关 母体类戊酰样α-淀粉酶（根据结构或功能考虑进行评估），ii）构建与母体类戊酰样α-淀粉酶相比的类戊酰样α-淀粉酶变体，已经在 在i）中鉴定的氨基酸残基或结构部分，以便改变其性质，和iii）测试所得到的类戊酰样α-淀粉酶变体。

    Amylase variants
    5.
    发明授权
    Amylase variants 失效
    淀粉酶变体

    公开(公告)号：US6093562A

    公开(公告)日：2000-07-25

    申请号：US600656

    申请日：1996-02-13

    申请人： Henrik Bisg.ang.rd-Frantzen ,  Allan Svendsen ,  Torben Vedel Borchert

    发明人： Henrik Bisg.ang.rd-Frantzen ,  Allan Svendsen ,  Torben Vedel Borchert

    IPC分类号： C11D3/386 ,  C12N9/28 ,  C12N15/56 ,  D06L1/14

    CPC分类号： C11D3/38618 ,  C11D3/38609 ,  C11D3/38681 ,  C12N9/2417 ,  C12Y302/01001 ,  D06L1/14

    摘要： The present invention relates to variants of a parent .alpha.-amylase, which parent .alpha.-amylase (i) has an amino acid sequence selected from the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3, and SEQ ID No. 7, respectively; or (ii) displays at least 80% homology with one or more of these amino acid sequences; and/or displays immunological cross-reactivity with an antibody raised against an .alpha.-amylase having one of these amino acid sequences; and/or is encoded by a DNA sequence which hybridizes with the same probe as a DNA sequence encoding an .alpha.-amylase having one of these amino acid sequences; in which variant:(a) at least one amino acid residue of the parent .alpha.-amylase has been deleted; and/or(b) at least one amino acid residue of the parent .alpha.-amylase has been replaced by a different amino acid residue; and/or(c) at least one amino acid residue has been inserted relative to the parent .alpha.-amylase; the variant having .alpha.-amylase activity and exhibiting at least one of the following properties relative to the parent .alpha.-amylase: increased thermostability; increased stability towards oxidation; and reduced Ca.sup.2+ dependency;with the proviso that the amino acid sequence of the variant is not identical to any of the amino acid sequences shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3 and SEQ ID No. 7, respectively.

    摘要翻译： 本发明涉及母体α-淀粉酶的变体，其母体α-淀粉酶（i）具有选自SEQ ID No.1，SEQ ID No.2，SEQ ID No.2所示的氨基酸序列的氨基酸序列。 3和SEQ ID No.7; 或（ii）与这些氨基酸序列中的一个或多个显示至少80％的同源性; 和/或显示与针对具有这些氨基酸序列之一的α-淀粉酶产生的抗体的免疫交叉反应性; 和/或由与编码具有这些氨基酸序列之一的α-淀粉酶的DNA序列相同的探针杂交的DNA序列编码; 在该变体中：（a）母体α-淀粉酶的至少一个氨基酸残基已被缺失; 和/或（b）母体α-淀粉酶的至少一个氨基酸残基被不同的氨基酸残基替代; 和/或（c）相对于母体α-淀粉酶插入至少一个氨基酸残基; 所述变体具有α-淀粉酶活性并且相对于母体α-淀粉酶显示出至少一种以下性质：增加的热稳定性; 提高氧化稳定性; 并减少Ca2 +依赖; 条件是变体的氨基酸序列分别与SEQ ID No.1，SEQ ID No.2，SEQ ID No.3和SEQ ID No.7所示的任何氨基酸序列不同。

    Amylase variants
    6.
    发明授权
    Amylase variants 失效
    淀粉酶变体

    公开(公告)号：US5830837A

    公开(公告)日：1998-11-03

    申请号：US343804

    申请日：1994-11-22

    申请人： Henrik Bisg.ang.rd-Frantzen ,  Torben Vedel Borchert ,  Allan Svendsen ,  Marianne Thellersen ,  Pia Van der Zee

    发明人： Henrik Bisg.ang.rd-Frantzen ,  Torben Vedel Borchert ,  Allan Svendsen ,  Marianne Thellersen ,  Pia Van der Zee

    IPC分类号： C11D3/386 ,  C12N9/28

    CPC分类号： C11D3/386

    摘要： A variant of a parent .alpha.-amylase enzyme having an improved washing and/or dishwashing performance as compared to the parent enzyme, wherein one or more amino acid residues of the parent enzyme have been replaced by a different amino acid residue and/or wherein one or more amino acid residues of the parent .alpha.-amylase have been deleted and/or wherein one or more amino acid residues have been added to the parent .alpha.-amylase enzyme, provided that the variant is different from one in which the methionine residue in position 197 of a parent B. licheniformis .alpha.-amylase has been replaced by alanine or threonine, as the only modification being made. The variant may be used for washing and dishwashing.

    摘要翻译： 与母体酶相比，具有改善的洗涤和/或餐具洗涤性能的母体α-淀粉酶的变体，其中母体酶的一个或多个氨基酸残基已被不同的氨基酸残基替代和/或其中一个 或更多的母体α-淀粉酶的氨基酸残基已经缺失和/或其中一个或多个氨基酸残基已加入到母体α-淀粉酶中，条件是该变体不同于其中位置上的甲硫氨酸残基的变体 作为唯一的修改，母本地衣芽孢杆菌α-淀粉酶的197已被丙氨酸或苏氨酸代替。 该变体可用于洗涤和洗碗。