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    Process for producing L-phenylalanine
    2.
    发明授权
    Process for producing L-phenylalanine 失效
    生产L-苯丙氨酸的方法

    公开(公告)号：US4755466A

    公开(公告)日：1988-07-05

    申请号：US697576

    申请日：1985-02-01

    申请人： Kenzo Yokozeki ,  Norimasa Onishi ,  Hideo Kano ,  Yoshiteru Hirose

    发明人： Kenzo Yokozeki ,  Norimasa Onishi ,  Hideo Kano ,  Yoshiteru Hirose

    IPC分类号： C12P13/22 ,  C12N1/14 ,  C12N1/16 ,  C12R1/645

    CPC分类号： C12R1/645 ,  C12P13/222 ,  Y10S435/911 ,  Y10S435/913

    摘要： A process for producing L-phenylalanine, which comprises reacting cinnamic acid and an ammonia generator in an aqueous medium in the presence of an enzyme or enzymes from a microorganism belonging to the genus Geotrichum, Moniliella, Syncephalastrum, Endomyces, Aspergillus, Saccharomycopis, Eurotium, Glomerella, Pellicularia or Conatobotyrum, wherein the enzyme or enzymes are capable of producing L-phenylalanine from cinnamic acid and an ammonia generator, and collecting L-phenylalanine from the aqueous medium, is disclosed along with both new and old microorganisms that can be used in the practice of the invention.

    摘要翻译： 一种生产L-苯丙氨酸的方法，其包括在水性介质中，在酶或者来自属于地热属，莫利菌属，Syncephalastrum，Endomyces，曲霉属，酵母属，欧洲，属的微生物的酶的存在下，使肉桂酸和氨发生剂反应， Glomerella，Pellicularia或Conatobotyrum，其中酶或酶能够从肉桂酸和氨发生器产生L-苯丙氨酸，并从水性介质中收集L-苯丙氨酸，以及可以用于 本发明的实践。

    DNA encoding hydantoinase, DNA encoding N-carbamyl-L-amino acid hydrolase, recombinant DNA, transformed cell, method of producing protein and method of producing optically active amino acid
    6.
    发明申请
    DNA encoding hydantoinase, DNA encoding N-carbamyl-L-amino acid hydrolase, recombinant DNA, transformed cell, method of producing protein and method of producing optically active amino acid 失效
    编码乙内酰脲的DNA，编码N-氨基甲酰-L-氨基酸水解酶的DNA，重组DNA，转化细胞，产生蛋白质的方法和产生旋光氨基酸的方法

    公开(公告)号：US20060240532A1

    公开(公告)日：2006-10-26

    申请号：US11476572

    申请日：2006-06-29

    申请人： Yasuhiro Takenaka ,  Shunichi Suzuki ,  Norimasa Onishi ,  Kenzo Yokozeki

    发明人： Yasuhiro Takenaka ,  Shunichi Suzuki ,  Norimasa Onishi ,  Kenzo Yokozeki

    IPC分类号： C12P13/04 ,  C12P13/22 ,  C07H21/04 ,  C12N9/80 ,  C12N15/74 ,  C12N1/21

    CPC分类号： C12N9/80 ,  C12N9/86 ,  C12P13/04 ,  C12P41/009 ,  C12Y305/01077 ,  C12Y305/02002

    摘要： The present invention provides a method of producing optically active amino acids from 5-substituted hydantoin by isolating a hydantoinase gene and an N-carbamyl-L-amino acid hydrolase gene involved in an ability to convert 5-substituted hydantoin or N-carbamylamino acid into optically active amino acids from a microorganism of the genus Microbacterium having the above ability and by improving gene amplification and transcriptional and translational activities thereby preparing a recombinant wherein the amount of the desired enzymes produced is increased. The hydantoinase gene is, for example, a DNA encoding for a protein having a hydantoinase activity, which has the nucleotide sequence set forth in SEQ ID NO:1 in the Sequence. The N-carbamyl-L-amino acid hydrolase gene is, for example, a DNA encoding for a protein having an N-carbamyl-L-amino acid hydrolase activity, which has the nucleotide sequence set forth in SEQ ID NO:3 in the Sequence.

    摘要翻译： 本发明提供了一种通过分离涉及将5-取代的乙内酰脲或N-氨基甲酰基氨基酸转化成5-取代的乙内酰脲或N-氨基甲酰基氨基酸的能力的乙内酰脲酶基因和N-氨基甲酰基-L-氨基酸水解酶基因从5-取代的乙内酰脲生产旋光性氨基酸的方法 具有上述能力的微杆菌属的微生物的旋光活性氨基酸，通过改善基因扩增和转录和翻译活性，从而制备重组体，其中产生的所需酶的量增加。 乙内酰脲酶基因是例如编码具有乙内酰脲酶活性的蛋白质的DNA，其具有序列中SEQ ID NO：1所示的核苷酸序列。 N-氨基甲酰基-L-氨基酸水解酶基因是例如编码具有N-氨基甲酰基-L-氨基酸水解酶活性的蛋白质的DNA，其具有SEQ ID NO：3所示的核苷酸序列 序列。

    DNA encoding hydantoinase, DNA encoding N-carbamyl-L-amino acid hydrolase, recombinant DNA, transformed cell, method of producing protein, and method of producing optically active amino acid
    8.
    发明授权
    DNA encoding hydantoinase, DNA encoding N-carbamyl-L-amino acid hydrolase, recombinant DNA, transformed cell, method of producing protein, and method of producing optically active amino acid 失效
    编码乙内酰脲的DNA，编码N-氨基甲酰基-L-氨基酸水解酶的DNA，重组DNA，转化细胞，产生蛋白质的方法以及制备光学活性氨基酸的方法

    公开(公告)号：US08460902B1

    公开(公告)日：2013-06-11

    申请号：US12035525

    申请日：2008-02-22

    申请人： Yasuhiro Takenaka ,  Shunichi Suzuki ,  Norimasa Onishi ,  Kenzo Yokozeki

    发明人： Yasuhiro Takenaka ,  Shunichi Suzuki ,  Norimasa Onishi ,  Kenzo Yokozeki

    IPC分类号： C12N15/55 ,  C12N9/80 ,  C12P13/04 ,  C12P13/22

    CPC分类号： C12N9/80 ,  C12N9/86 ,  C12P13/04 ,  C12P41/009 ,  C12Y305/01077 ,  C12Y305/02002

    摘要： The present invention provides a method of producing optically active amino acids from 5-substituted hydantoin by isolating a hydantoinase gene and an N-carbamyl-L-amino acid hydrolase gene involved in an ability to convert 5-substituted hydantoin or N-carbamylamino acid into optically active amino acids from a microorganism of the genus Microbacterium having the above ability and by improving gene amplification and transcriptional and translational activities thereby preparing a recombinant wherein the amount of the desired enzymes produced is increased. The hydantoinase gene is, for example, a DNA encoding for a protein having a hydantoinase activity, which has the nucleotide sequence of SEQ ID NO:1. The N-carbamyl-L-amino acid hydrolase gene is, for example, a DNA encoding for a protein having an N-carbamyl-L-amino acid hydrolase activity, which has the nucleotide sequence of SEQ ID NO:3.

    摘要翻译： 本发明提供了一种通过分离涉及将5-取代的乙内酰脲或N-氨基甲酰基氨基酸转化成5-取代的乙内酰脲或N-氨基甲酰基氨基酸的能力的乙内酰脲酶基因和N-氨基甲酰基-L-氨基酸水解酶基因从5-取代的乙内酰脲生产旋光性氨基酸的方法 具有上述能力的微杆菌属的微生物的旋光活性氨基酸，通过改善基因扩增和转录和翻译活性，从而制备重组体，其中产生的所需酶的量增加。 乙内酰脲酶基因是例如编码具有乙内酰脲酶活性的蛋白质的DNA，其具有SEQ ID NO：1的核苷酸序列。 N-氨基甲酰基-L-氨基酸水解酶基因是例如编码具有N-氨基甲酰基-L-氨基酸水解酶活性的蛋白质的DNA，其具有SEQ ID NO：3的核苷酸序列。

    DNA encoding hydantoinase, DNA encoding N-carbamyl-L-amino acid hydrolase, recombinant DNA, transformed cell, method of producing protein and method of producing optically active amino acid
    9.
    发明授权
    DNA encoding hydantoinase, DNA encoding N-carbamyl-L-amino acid hydrolase, recombinant DNA, transformed cell, method of producing protein and method of producing optically active amino acid 失效
    编码乙内酰脲的DNA，编码N-氨基甲酰-L-氨基酸水解酶的DNA，重组DNA，转化细胞，产生蛋白质的方法和产生旋光氨基酸的方法

    公开(公告)号：US07361490B2

    公开(公告)日：2008-04-22

    申请号：US11476572

    申请日：2006-06-29

    申请人： Yasuhiro Takenaka ,  Shunichi Suzuki ,  Norimasa Onishi ,  Kenzo Yokozeki

    发明人： Yasuhiro Takenaka ,  Shunichi Suzuki ,  Norimasa Onishi ,  Kenzo Yokozeki

    IPC分类号： C12P13/22 ,  C12P13/04 ,  C12N9/86

    CPC分类号： C12N9/80 ,  C12N9/86 ,  C12P13/04 ,  C12P41/009 ,  C12Y305/01077 ,  C12Y305/02002

    摘要： The present invention provides a method of producing optically active amino acids from 5-substituted hydantoin by isolating a hydantoinase gene and an N-carbamyl-L-amino acid hydrolase gene involved in an ability to convert 5-substituted hydantoin or N-carbamylamino acid into optically active amino acids from a microorganism of the genus Microbacterium having the above ability and by improving gene amplification and transcriptional and translational activities thereby preparing a recombinant wherein the amount of the desired enzymes produced is increased. The hydantoinase gene is, for example, a DNA encoding for a protein having a hydantoinase activity, which has the nucleotide sequence set forth in SEQ ID NO:1 in the Sequence. The N-carbamyl-L-amino acid hydrolase gene is, for example, a DNA encoding for a protein having an N-carbamyl-L-amino acid hydrolase activity, which has the nucleotide sequence set forth in SEQ ID NO:3 in the Sequence.

    摘要翻译： 本发明提供了一种通过分离涉及将5-取代的乙内酰脲或N-氨基甲酰基氨基酸转化成5-取代的乙内酰脲或N-氨基甲酰基氨基酸的能力的乙内酰脲酶基因和N-氨基甲酰基-L-氨基酸水解酶基因从5-取代的乙内酰脲生产旋光性氨基酸的方法 具有上述能力的微杆菌属的微生物的旋光活性氨基酸，通过改善基因扩增和转录和翻译活性，从而制备重组体，其中产生的所需酶的量增加。 乙内酰脲酶基因是例如编码具有乙内酰脲酶活性的蛋白质的DNA，其具有序列中SEQ ID NO：1所示的核苷酸序列。 N-氨基甲酰基-L-氨基酸水解酶基因是例如编码具有N-氨基甲酰基-L-氨基酸水解酶活性的蛋白质的DNA，其具有SEQ ID NO：3所示的核苷酸序列 序列。