公开(公告)号：US11162065B2

公开(公告)日：2021-11-02

申请号：US16739573

申请日：2020-01-10

申请人： Novartis AG ,  The Trustees of the University of Pennsylvania

发明人： Fabio Fachin ,  Rodney Rietze ,  Michael R. Greene ,  Lan Cao

IPC分类号： C12M1/00 ,  B03C1/30 ,  C12M1/12 ,  B03C1/033 ,  B03C1/28 ,  B03C1/01 ,  C07K16/28 ,  C12N13/00 ,  A61K39/00

摘要： The present disclosure relates to systems and methods for flow-through separation of paramagnetic particle-bound cells in a cell suspension containing both bound and unbound cells as well as systems and methods for removing paramagnetic particles from paramagnetic particle-bound cells or from a cell suspension with unbound cells. It further relates to a flow-through magnetic separation/debeading module and a flow-through spinning membrane debeading module.

公开(公告)号：US20200248130A1

公开(公告)日：2020-08-06

申请号：US16739573

申请日：2020-01-10

申请人： Novartis AG ,  The Trustees of the University of Pennsylvania

发明人： Fabio Fachin ,  Rodney Rietze ,  Michael R. Greene ,  Lan Cao

IPC分类号： C12M1/00 ,  B03C1/01 ,  B03C1/28 ,  B03C1/033 ,  B03C1/30 ,  C12N13/00 ,  C12M1/12 ,  C07K16/28

摘要： The present disclosure relates to systems and methods for flow-through separation of paramagnetic particle-bound cells in a cell suspension containing both bound and unbound cells as well as systems and methods for removing paramagnetic particles from paramagnetic particle-bound cells or from a cell suspension with unbound cells. It further relates to a flow-through magnetic separation/debeading module and a flow-through spinning membrane debeading module.

公开(公告)号：US11912978B2

公开(公告)日：2024-02-27

申请号：US17503186

申请日：2021-10-15

申请人： Novartis AG ,  The Trustees of the University of Pennsylvania

发明人： Fabio Fachin ,  Rodney Rietze ,  Michael R. Greene ,  Lan Cao

IPC分类号： C12M1/00 ,  B03C1/01 ,  B03C1/033 ,  B03C1/28 ,  B03C1/30 ,  C07K16/28 ,  C12M1/12 ,  C12N13/00 ,  A61K39/00

CPC分类号： C12M47/04 ,  B03C1/01 ,  B03C1/0332 ,  B03C1/288 ,  B03C1/30 ,  C07K16/2803 ,  C07K16/2809 ,  C07K16/2818 ,  C12M25/02 ,  C12N13/00 ,  A61K2039/505

摘要： The present disclosure relates to systems and methods for flow-through separation of paramagnetic particle-bound cells in a cell suspension containing both bound and unbound cells as well as systems and methods for removing paramagnetic particles from paramagnetic particle-bound cells or from a cell suspension with unbound cells. It further relates to a flow-through magnetic separation/debeading module and a flow-through spinning membrane debeading module.

公开(公告)号：US20160355777A1

公开(公告)日：2016-12-08

申请号：US15172415

申请日：2016-06-03

申请人： Novartis AG ,  The Trustees of the University of Pennsylvania

发明人： Fabio Fachin ,  Rodney Rietze ,  Michael R. Greene ,  Lan Cao

IPC分类号： C12M1/00 ,  C12N13/00 ,  C12M1/12 ,  C07K16/28

CPC分类号： C12M47/04 ,  A61K2039/505 ,  B03C1/01 ,  B03C1/0332 ,  B03C1/288 ,  B03C1/30 ,  C07K16/2803 ,  C07K16/2809 ,  C07K16/2818 ,  C12M25/02 ,  C12N13/00

摘要： The present disclosure relates to systems and methods for flow-through separation of paramagnetic particle-bound cells in a cell suspension containing both bound and unbound cells as well as systems and methods for removing paramagnetic particles from paramagnetic particle-bound cells or from a cell suspension with unbound cells. It further relates to a flow-through magnetic separation/debeading module and a flow-through spinning membrane debeading module.

摘要翻译： 本公开涉及用于在含有结合和未结合细胞的细胞悬浮液中的顺磁性颗粒结合细胞的流通分离的系统和方法，以及用于从顺磁颗粒结合的细胞或细胞悬液中除去顺磁性颗粒的系统和方法 与未绑定的单元格。 它还涉及一种流通磁分离/去带模块和一个流通纺丝膜去壳模块。

公开(公告)号：US20230174933A1

公开(公告)日：2023-06-08

申请号：US17801665

申请日：2021-02-26

申请人： Novartis AG

发明人： Jennifer BROGDON ,  Seth CARBONNEAU ,  Glenn DRANOFF ,  Michael R. Greene ,  Anniesha HACK ,  Marc Horst Peter HILD ,  Olja KODRASI ,  Elizabeth Dorothy PRATICO ,  Andrew PRICE ,  Andrew Marc STEIN ,  Attilio BONDANZA ,  Boris ENGELS ,  Carla Patricia Pinto GUIMARAES ,  Hyungwook LIM ,  Sujata SHARMA ,  Akash SOHONI ,  Louise TREANOR ,  Xu ZHU

IPC分类号： C12N5/0783 ,  A61K35/17 ,  A61P35/00 ,  C07K16/28 ,  C07K14/705 ,  C07K14/725 ,  C07K14/47 ,  C07K14/71 ,  A61K38/17 ,  A61K39/395 ,  A61K45/06 ,  A61P35/02

CPC分类号： C12N5/0636 ,  A61K35/17 ,  A61P35/00 ,  C07K16/2878 ,  C07K14/70517 ,  C07K14/7051 ,  C07K14/70578 ,  C07K14/4702 ,  C07K14/71 ,  A61K38/1774 ,  A61K38/177 ,  A61K38/1709 ,  A61K38/179 ,  A61K39/39558 ,  A61K45/06 ,  C07K16/2803 ,  A61P35/02 ,  C07K2319/33 ,  A61K2039/505

摘要： This disclosure provides methods of making immune effector cells (for example, T cells, NK cells) that comprise (i) a nucleic acid molecule that encodes a controllable chimeric antigen receptor (CCAR) or (ii) a nucleic acid molecule that encodes a CAR and a regulatory molecule, and compositions generated by such methods.

公开(公告)号：US20190269727A1

公开(公告)日：2019-09-05

申请号：US16066788

申请日：2016-12-27

申请人： Fabio Fachin ,  Lan Cao ,  Michael R. Greene ,  Tatiana Golovina ,  Novartis AG ,  The Trustees of the University of Pennsylvania

发明人： Fabio Fachin ,  Lan Cao ,  Michael R. Greene ,  Tatiana Golovina

IPC分类号： A61K35/17 ,  C07K14/725 ,  C12N5/0783 ,  C07K1/04 ,  A61M1/34 ,  A61M1/36

摘要： The invention provides methods of making immune effector cells (e.g., T cells, NK cells) that can be engineered to express a chimeric antigen receptor (CAR), and compositions and reaction mixtures comprising the same.

公开(公告)号：US20240024360A1

公开(公告)日：2024-01-25

申请号：US18460977

申请日：2023-09-05

申请人： Novartis AG ,  The Trustees of the University of Pennsylvania

发明人： Fabio Fachin ,  Lan Cao ,  Michael R. Greene ,  Tatiana Golovina

IPC分类号： A61K35/17 ,  A61M1/34 ,  A61M1/36 ,  C07K1/04 ,  C07K14/725 ,  C12N5/0783

CPC分类号： A61K35/17 ,  A61M1/3486 ,  A61M1/3618 ,  A61M1/3496 ,  A61M1/3693 ,  C07K1/042 ,  C07K14/7051 ,  C12N5/0636

摘要： The invention provides methods of making immune effector cells (e.g., T cells, NK cells) that can be engineered to express a chimeric antigen receptor (CAR), and compositions and reaction mixtures comprising the same.

公开(公告)号：US20220364055A1

公开(公告)日：2022-11-17

申请号：US17271430

申请日：2019-08-30

申请人： Novartis AG

发明人： Louise Treanor ,  Michael R. Greene ,  Jennifer Brogdon ,  Boris Engels ,  Glenn Dranoff ,  Olja Kodrasi ,  Hyungwook Lim ,  Akash Sohoni ,  Elizabeth Dorothy Pratico ,  Anniesha Hack ,  Aida Abujoub ,  Tony Fleming ,  Lu Huang ,  Connie Hong ,  John Blankenship ,  Brian Holmberg ,  Chonghui Zhang ,  Dexiu Bu ,  Andrew Price ,  Xu Zhu ,  Andrew Stein ,  Attilio Bondanza

IPC分类号： C12N5/0783 ,  A61K35/17 ,  A61K31/519 ,  A61K38/20 ,  C07K16/28 ,  C07K14/705

摘要： The invention provides methods of making immune effector cells (for example, T cells, NK cells) that express a chimeric antigen receptor (CAR), and compositions generated by such methods.

公开(公告)号：US20210220404A1

公开(公告)日：2021-07-22

申请号：US17104983

申请日：2020-11-25

申请人： Michael R. Greene ,  Novartis AG

发明人： Aida Abujoub ,  John Blankenship ,  Attilio Bondanza ,  Jennifer Brogdon ,  Dexiu Bu ,  Serena De Vita ,  Glenn Dranoff ,  Boris Engels ,  Tony Fleming ,  Brian Walter Granda ,  Michael R. Greene ,  Carla Patricia Guimaraes ,  Anniesha Hack ,  Brian Holmberg ,  Connie Hong ,  Lu Huang ,  Olja Kodrasi ,  Joni Waiee Lam ,  Hyungwook Lim ,  Elizabeth Dorothy Pratico ,  Andrew Price ,  Akash Sohoni ,  Andrew Marc Stein ,  Louise Treanor ,  Chonghui Zhang ,  Xu Zhu

IPC分类号： A61K35/17 ,  C07K16/28 ,  A61P35/00 ,  A61K45/06 ,  A61K39/395 ,  C07K14/705 ,  A61K38/17 ,  C12N5/0783 ,  C07K14/725

摘要： The invention provides immune effector cells (for example, T cells, NK cells) that express a chimeric antigen receptor (CAR), and compositions and methods thereof.