公开(公告)号：US20240307869A1

公开(公告)日：2024-09-19

申请号：US18576158

申请日：2022-06-21

Applicant: OPTOLANE TECHNOLOGIES INC.

Inventor： Seung Sun CHO ,  An Shik CHOI ,  Byoung Joo KWAK ,  Sergiy OLEKSANDROV

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L3/502707 ,  B01L2200/0642 ,  B01L2300/044 ,  B01L2300/0663 ,  B01L2300/0819 ,  B01L2300/161 ,  B01L2400/0406

Abstract: Disclosed is a PCR module in which a sample can be injected using the capillary effect. The PCR module comprises: a microfluidic chamber which includes an inlet part for inputting a sample and is manufactured by injection molding; a well array which is disposed on the lower surface of the microfluidic chamber and includes a plurality of micro-wells of which the upper and lower portions are perforated; and a capillary member providing a path so that the sample input through the inlet part reaches the micro-wells through the capillary effect. When moving a PCR solution to a reaction space using the capillary effect and filling the reaction space with the solution, it is possible to prevent air pockets from forming in the corners or edge regions of the well array which is the reaction space. Accordingly, it is possible to prevent errors caused by air pockets from occurring in PCR test results. In addition, since the well array is located on a CMOS photosensor array, PCR reactions can be measured in real time.

公开(公告)号：US20160271611A1

公开(公告)日：2016-09-22

申请号：US15064816

申请日：2016-03-09

Applicant: OPTOLANE Technologies Inc.

Inventor： DoYoung LEE ,  In Gyun JEON ,  An Shik CHOI ,  Kyung Hak CHOI

IPC: B01L7/00 ,  C12Q1/68

CPC classification number: B01L7/52 ,  B01L3/50851 ,  B01L2200/028 ,  B01L2200/147 ,  B01L2300/0663 ,  B01L2300/0681 ,  B01L2300/0819 ,  B01L2300/18 ,  B01L2300/1805 ,  B01L2300/1822 ,  B01L2300/1844 ,  B01L2300/185 ,  C12Q1/686 ,  G01N21/64

Abstract: A polymerase chain reaction (PCR) module is detachably combined with a reader system. The reader system includes a central processing unit (CPU) receiving a photo sensing signal to calculate gene amplification amount in real time and generating a temperature control signal based on a temperature signal and a temperature control information. The PCR module includes a photo sensor assembly, a partition wall, and an interface module. The photo sensor assembly includes a plurality of photo sensors and a temperature sensor. The photo sensors are arranged in an array shape to sense emission light generated from a specimen to generate the photo sensing signal. The partition wall is protruded from the photo sensor assembly to define a reaction space in which the specimen is received. The interface module is electrically connected to the photo sensor assembly to transmit the photo sensing signal and the temperature signal to the reader system.

Abstract translation: 聚合酶链反应（PCR）模块可与读取器系统可拆卸地组合。 读取器系统包括中央处理单元（CPU），其接收感光信号以实时计算基因放大量，并且基于温度信号和温度控制信息产生温度控制信号。 PCR模块包括光传感器组件，分隔壁和接口模块。 光传感器组件包括多个光传感器和温度传感器。 光传感器被布置成阵列形状以检测从样本产生的发射光以产生光感测信号。 分隔壁从光传感器组件突出以限定其中接收样品的反应空间。 接口模块电连接到光传感器组件，以将感光信号和温度信号传输到读取器系统。

公开(公告)号：US20190217303A1

公开(公告)日：2019-07-18

申请号：US16361594

申请日：2019-03-22

Applicant: OPTOLANE Technologies Inc.

Inventor： DoYoung LEE ,  In Gyun JEON ,  An Shik CHOI ,  Kyung Hak CHOI

IPC: B01L7/00 ,  B01L3/00 ,  G01N21/64 ,  C12Q1/686

Abstract: A polymerase chain reaction (PCR) module is detachably combined with a reader system. The reader system includes a central processing unit (CPU) receiving a photo sensing signal to calculate gene amplification amount in real time and generating a temperature control signal based on a temperature signal and a temperature control information. The PCR module includes a photo sensor assembly, a partition wall, and an interface module. The photo sensor assembly includes a plurality of photo sensors and a temperature sensor. The photo sensors are arranged in an array shape to sense emission light generated from a specimen to generate the photo sensing signal. The partition wall is protruded from the photo sensor assembly to define a reaction space in which the specimen is received. The interface module is electrically connected to the photo sensor assembly to transmit the photo sensing signal and the temperature signal to the reader system.

公开(公告)号：US20240286126A1

公开(公告)日：2024-08-29

申请号：US18573475

申请日：2022-06-10

Applicant: OPTOLANE TECHNOLOGIES INC.

Inventor： Seung Sun CHO ,  An Shik CHOI ,  Byoung Joo KWAK ,  Sergiy OLEKSANDROV ,  Jae Hun KIM

IPC: B01L3/00

CPC classification number: B01L3/502 ,  B01L2200/0684 ,  B01L2200/14 ,  B01L2300/042 ,  B01L2300/0829 ,  B01L2300/161 ,  B01L2400/088

Abstract: Disclosed is an in vitro diagnostic chip having multiple levels of hydrophilicity, which differentially controls the flow rates of a liquid sample and thus enables the liquid sample to be evenly distributed all the way to corner or edge regions of a reaction space. The in vitro diagnostic chip having multiple levels of hydrophilicity comprises: a well array comprising a plurality of wells; and a multi-hydrophilic coating layer coated on the well array and controlling the flow rates of a sample so as to be mutually different. The hydrophilic coating layer is formed on the well array so as to have high hydrophilicity in accordance with the center region of the well array, and to have low hydrophilicity in accordance with the peripheral region of the well array, and thus the formation of an air pocket in the reaction space may be blocked. Accordingly, the occurrence of an error caused by the air pocket in a PCR test result may be prevented.