公开(公告)号：US20160138101A1

公开(公告)日：2016-05-19

申请号：US14883494

申请日：2015-10-14

Applicant: Osaka University

Inventor： Masateru Taniguchi ,  Makusu Tsutsui ,  Kazumichi Yokota ,  Tomoji Kawai

IPC: C12Q1/68 ,  G01N27/327

CPC classification number: C12Q1/6874 ,  C12Q1/6869 ,  G01N27/3278 ,  C12Q2565/631 ,  C12Q2565/607 ,  C12Q2523/303 ,  C12Q2563/116 ,  C12Q2563/157

Abstract: The present invention provides technology that uses current measurements to identify nucleotides and determine a nucleotide sequence in polynucleotides. The present invention calculates a modal value of a tunnel current that arises when a nucleotide or polynucleotide for analysis passes through between electrodes, and then employs the calculated modal value. The present invention accordingly enables direct rapid implementation to identify nucleotides and to determine a nucleotide sequence in a polynucleotide without marking.

Abstract translation: 本发明提供了使用当前测量来鉴定核苷酸并确定多核苷酸中的核苷酸序列的技术。 本发明计算当用于分析的核苷酸或多核苷酸通过电极之间时产生的隧道电流的模态值，然后采用所计算的模态值。 因此，本发明使得能够直接快速实施鉴定核苷酸并确定多核苷酸中的核苷酸序列，而无需标记。

公开(公告)号：US12013365B2

公开(公告)日：2024-06-18

申请号：US17258662

申请日：2019-07-18

Applicant: OSAKA UNIVERSITY

Inventor： Kohji Nishida ,  Noriyasu Hashida ,  Masateru Taniguchi ,  Makusu Tsutsui

IPC: G01N27/327 ,  G01N27/447 ,  G01N33/487 ,  G01N33/569

CPC classification number: G01N27/3278 ,  G01N27/447 ,  G01N33/48721 ,  G01N33/56983

Abstract: Provided are a virus measuring method, a virus measuring device, a virus determining program, a stress determining method, and a stress determining device. A virus measuring method includes a contact step of bringing a liquid specimen containing a body fluid of a subject and an electrolytic solution into contact with each other via a through-hole portion formed in a separating wall, a current measuring step of applying a voltage to the liquid specimen and the electrolytic solution with respect to the through-hole portion and obtaining a waveform of an ionic current flowing through the through-hole portion, and a virus determining step of determining the kind of a virus contained in the body fluid on the basis of the waveform. In the virus determining step, the kind of the virus is determined by comparing the waveform with waveform information that corresponds to a known virus and is obtained beforehand.

公开(公告)号：US20190242846A1

公开(公告)日：2019-08-08

申请号：US16266363

申请日：2019-02-04

Applicant: Quantum Biosystems Inc. ,  Osaka University

Inventor： Masateru TANIGUCHI ,  Takahito Ohshiro ,  Makusu Tsutsui ,  Kazumichi Yokota ,  Toshiki Matsuoka ,  Mark Oldham ,  Eric Nordman

IPC: G01N27/327 ,  G01N33/487

CPC classification number: G01N27/3278 ,  B82Y40/00 ,  C12M1/00 ,  C12Q1/6869 ,  G01N33/48721

Abstract: Methods and systems are provided for creation of stable and consistent nanoelectrode pairs for detection of biomolecules, such as deoxyribonucleic acid.

公开(公告)号：US09726636B2

公开(公告)日：2017-08-08

申请号：US14484305

申请日：2014-09-12

Applicant: KABUSHIKI KAISHA TOSHIBA ,  OSAKA UNIVERSITY

Inventor： Sadato Hongo ,  Tomoji Kawai ,  Makusu Tsutsui ,  Masateru Taniguchi ,  Soh Ryuzaki

IPC: G01N27/447 ,  G01N15/12 ,  G01N33/487 ,  G01N15/10

CPC classification number: G01N27/44756 ,  G01N15/12 ,  G01N15/1218 ,  G01N33/48721 ,  G01N2015/1062 ,  G01N2015/1087 ,  G01N2015/1093

Abstract: According to one embodiment, provided is a single particle analyzing device including a measuring vessel, first and second chambers in the vessel defined by an insulating membrane, a pore opening in the membrane to connect the chambers, and first and second electrodes in the chambers. Electric current flows between the electrodes through the pore. Electrical characteristics are measured during migration of the target from the first chamber to the second chamber to measure the size and shape of the target. (a) t