公开(公告)号：US20230194472A1

公开(公告)日：2023-06-22

申请号：US17605917

申请日：2020-04-24

Applicant: Osaka University

Inventor： Masateru Taniguchi ,  Hideshi Ishii ,  Takahito Ohshiro ,  Masamitsu Konno

IPC: G01N27/62 ,  C12Q1/6886

CPC classification number: G01N27/62 ,  C12Q1/6886

Abstract: The present disclosure provides a method for analyzing a microRNA using a tunneling current. The present disclosure provides a method for identifying the base sequence and/or modification state of a microRNA using a tunneling current, and a system and a program to be used in the method. Furthermore, the present disclosure provides a method for analyzing the conditions of a subject, said method comprising determining the base sequence and/or modification state of a microRNA using a tunneling current. For example, methylation modification can be analyzed thereby.

公开(公告)号：US20170146510A1

公开(公告)日：2017-05-25

申请号：US15344184

申请日：2016-11-04

Applicant: Quantum Biosystems Inc. ,  Osaka University

Inventor： Shuji Ikeda ,  Masateru Taniguchi

IPC: G01N33/487 ,  G01N27/447 ,  C12Q1/68

CPC classification number: G01N33/48721 ,  C12Q1/6869 ,  G01N27/44791

Abstract: The present disclosure provides methods and structures for effectuating nanoelectrodes with an adjustable nanogap. Devices with integrated actuators (e.g., piezoelectric devices) and/or materials with different coefficients of expansion are described. Also described are methods for calibrations nanoelectrode pairs.

公开(公告)号：US10413903B2

公开(公告)日：2019-09-17

申请号：US15340584

申请日：2016-11-01

Applicant: Osaka University

Inventor： Masateru Taniguchi

IPC: C12Q1/6869 ,  B01L3/00 ,  C12Q1/6806 ,  B82Y10/00 ,  B82Y30/00

Abstract: The present disclosure provides methods and structures for systems which can linearize and capture a nucleic acid molecule (e.g., DNA) for re-measurement of the nucleic acid molecule or other polymer prior to detection of the polymer. The structures may allow for quick exchange between different samples or other reagents.

公开(公告)号：US20170306396A1

公开(公告)日：2017-10-26

申请号：US15242221

申请日：2016-08-19

Applicant: Osaka University

Inventor： Tomoji Kawai ,  Masateru Taniguchi ,  Takahito Ohshiro

IPC: C12Q1/68 ,  G01N27/447 ,  G01N33/487

CPC classification number: C12Q1/6869 ,  G01N27/44704 ,  G01N27/44791 ,  G01N33/48721

Abstract: The nano-gap electrode pair 12 is disposed so that a biomolecule joined to at least one or more types of a single molecule included in a sample passes an opposing position, and the strength of the electric field in a position spaced only a predetermined distance on the downstream side from the opposing position 64 becomes stronger than the strength of the electric field in a position spaced only the predetermined distance on the upstream side from the opposing position 64.

公开(公告)号：US12123848B2

公开(公告)日：2024-10-22

申请号：US17598726

申请日：2020-02-20

Applicant: OSAKA UNIVERSITY

Inventor： Masateru Taniguchi ,  Takahito Ohshiro ,  Masakazu Sanada ,  Tadashi Miyagi

IPC: G01N27/49 ,  G01N27/327 ,  G01N33/487

CPC classification number: G01N27/49 ,  G01N33/48721

Abstract: A current measurement method for measuring a tunneling current in biopolymers passing through between a pair of electrodes includes arranging the electrodes in a liquid that contains an electrolyte and, while applying a voltage between the electrodes, measuring a current flowing between the electrodes via an electric double layer formed along surfaces of the electrodes. This enables measuring the current in consideration of the electric double layer. As a result, it is possible to more accurately measure the tunneling current in the biopolymers included in a liquid sample that contains an electrolyte.

公开(公告)号：US20160138101A1

公开(公告)日：2016-05-19

申请号：US14883494

申请日：2015-10-14

Applicant: Osaka University

Inventor： Masateru Taniguchi ,  Makusu Tsutsui ,  Kazumichi Yokota ,  Tomoji Kawai

IPC: C12Q1/68 ,  G01N27/327

CPC classification number: C12Q1/6874 ,  C12Q1/6869 ,  G01N27/3278 ,  C12Q2565/631 ,  C12Q2565/607 ,  C12Q2523/303 ,  C12Q2563/116 ,  C12Q2563/157

Abstract: The present invention provides technology that uses current measurements to identify nucleotides and determine a nucleotide sequence in polynucleotides. The present invention calculates a modal value of a tunnel current that arises when a nucleotide or polynucleotide for analysis passes through between electrodes, and then employs the calculated modal value. The present invention accordingly enables direct rapid implementation to identify nucleotides and to determine a nucleotide sequence in a polynucleotide without marking.

Abstract translation: 本发明提供了使用当前测量来鉴定核苷酸并确定多核苷酸中的核苷酸序列的技术。 本发明计算当用于分析的核苷酸或多核苷酸通过电极之间时产生的隧道电流的模态值，然后采用所计算的模态值。 因此，本发明使得能够直接快速实施鉴定核苷酸并确定多核苷酸中的核苷酸序列，而无需标记。

公开(公告)号：US20170144158A1

公开(公告)日：2017-05-25

申请号：US15340584

申请日：2016-11-01

Applicant: Osaka University

Inventor： Masateru Taniguchi

IPC: B01L3/00

CPC classification number: B01L3/502761 ,  B01L2200/0663 ,  B01L2300/0645 ,  B01L2300/0896 ,  B01L2400/0415 ,  B01L2400/086 ,  B82Y10/00 ,  B82Y30/00 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2565/631

Abstract: The present disclosure provides methods and structures for systems which can linearize and capture a nucleic acid molecule (e.g., DNA) for re-measurement of the nucleic acid molecule or other polymer prior to detection of the polymer. The structures may allow for quick exchange between different samples or other reagents.

公开(公告)号：US20160319342A1

公开(公告)日：2016-11-03

申请号：US15061871

申请日：2016-03-04

Applicant: Osaka University ,  Quantum Biosystems Inc.

Inventor： Tomoji Kawai ,  Masateru Taniguchi ,  Takahito Ohshiro ,  Mark Oldham ,  Eric Nordman

IPC: C12Q1/68 ,  G01N27/04 ,  G06F19/22

CPC classification number: C12Q1/6869 ,  G01N27/04 ,  G01N33/48721 ,  G06F19/22 ,  C12Q2563/116 ,  C12Q2565/631

Abstract: Devices, systems and methods for sequencing protein samples are provided. In some examples, currents generated when a monomer passes through between electrodes of a nanogap electrode pair are measured for each of several different distances, so that monomers are identified when compared to a reference physical quantity of a known monomer, which may be obtained from a current measured with a similar inter-electrode distance(s) at which each of plural kinds of monomers are identifiable and ordered with predetermined accuracy and based on a detected physical quantity obtained from a tunneling current, which may be further normalized by the use of one or more reference substances.

Abstract translation: 提供了用于测序蛋白质样品的设备，系统和方法。 在一些实例中，当在几个不同距离中的每一个测量单体通过纳米缝电极对的电极之间产生的电流，使得当与已知单体的参考物理量相比时，识别单体，其可以从 以类似的电极间距测量的电流，其中可以以预定精度识别和排序多种单体中的每一种，并且基于从隧道电流获得的检测到的物理量，其可以通过使用一个 或更多的参考物质。

公开(公告)号：US20150219593A1

公开(公告)日：2015-08-06

申请号：US14421809

申请日：2013-08-02

Applicant: OSAKA UNIVERSITY

Inventor： Tomoji Kawai ,  Takahito Ohshiro ,  Masateru Taniguchi

IPC: G01N27/447

CPC classification number: G01N27/447 ,  G01N15/1031 ,  G01N27/3275 ,  G01N27/4473 ,  G01N33/48721 ,  G01N2015/1006

Abstract: The analysis method allows analysis of samples with high sensitivity, irrespective of interelectrode distance. The method includes: a step of applying a voltage between a first electrode pair such that an electric field is formed in a direction intersecting a migration direction of a sample; a step of placing a solution, including an electrochemically active molecule that produces a redox reaction at the electrode pair, between the first electrode pair; a step of causing the sample to migrate; and a step of measuring an amount of change in current flow between the first electrode pair.

Abstract translation: 分析方法允许分析具有高灵敏度的样品，而不考虑电极间距离。 该方法包括：在与样品的迁移方向相交的方向形成电场的第一电极对之间施加电压的步骤; 在第一电极对之间放置包括在电极对产生氧化还原反应的电化学活性分子的溶液的步骤; 使样品迁移的步骤; 以及测量第一电极对之间的电流变化量的步骤。

公开(公告)号：US20140183040A1

公开(公告)日：2014-07-03

申请号：US13975610

申请日：2013-08-26

Applicant: Osaka University

Inventor： Tomoji Kawai ,  Soh Ryuzaki ,  Masateru Taniguchi

IPC: G01N27/447

CPC classification number: G01N27/44756 ,  G01N27/44752 ,  G01N33/48721

Abstract: The present invention provides a method and apparatus for controlling the moving speed of a substance, both of which can adjust the moving speed of a substance to a desired speed. The control method and control apparatus cause a substance to pass through an internal space, in which an electro-osmotic flow is generated, of a surround electrode formed so as to surround part of the moving path of the substance, whereby the control method and control apparatus change the moving speed of the substance.

Abstract translation: 本发明提供了一种用于控制物质的移动速度的方法和装置，它们都可以将物质的移动速度调节到期望的速度。 控制方法和控制装置使物质通过形成为围绕物质的移动路径的一部分而形成的环境电极的产生电渗流的内部空间，由此控制方法和控制 设备改变物质的移动速度。