公开(公告)号：US20220411750A1

公开(公告)日：2022-12-29

申请号：US17777786

申请日：2020-11-17

Applicant: TOPPAN INC. ,  Osaka University

Inventor： Yasuyuki HIRAOKA ,  Shiro KITANO ,  Shinji IRIE ,  Michiya MATSUSAKI

IPC: C12N5/071

Abstract: The present invention relates to a cell structure including cells containing at least hepatocytes and vascular endothelial cells, and extracellular matrix component, wherein the extracellular matrix components are disposed between the cells, and a liver sinusoidal network is provided between the cells.

公开(公告)号：US20220145242A1

公开(公告)日：2022-05-12

申请号：US17430648

申请日：2021-03-25

Applicant: TOPPAN INC. ,  OSAKA UNIVERSITY

Inventor： Shiro KITANO ,  Shinji IRIE ,  Michiya MATSUSAKI

IPC: C12N5/00 ,  C07K14/78

Abstract: The present invention relates to an extracellular matrix-containing composition containing a fragmented extracellular matrix component and a compound bound or adsorbed to the fragmented extracellular matrix component.

公开(公告)号：US20230110381A1

公开(公告)日：2023-04-13

申请号：US17908283

申请日：2021-03-04

Applicant: OSAKA UNIVERSITY ,  TOPPAN INC.

Inventor： Michiya MATSUSAKI ,  Naoko SASAKI ,  Shiro KITANO ,  Shinji IRIE

IPC: A61L27/50 ,  A61L27/36 ,  A61L27/24 ,  A61L27/38 ,  C12N5/00

Abstract: Disclosed is a method for controlling the Young’s modulus of a three-dimensional tissue containing cells and an extracellular matrix by adjusting the average diameter of an extracellular matrix in production of the three-dimensional tissue.

公开(公告)号：US20220341918A1

公开(公告)日：2022-10-27

申请号：US17642608

申请日：2020-08-27

Applicant: TOPPAN INC. ,  Osaka University

Inventor： Shiro KITANO ,  Shinji IRIE ,  Michiya MATSUSAKI ,  Naoko SASAKI ,  Atsuo AMANO ,  Hiroki TAKEUCHI

IPC: G01N33/50 ,  C12N5/071

Abstract: Provided is a cell structure including: a connective tissue structure; and an epithelial structure placed on the connective tissue structure, in which the connective tissue structure contains a fragmented extracellular matrix component and first cells including mesenchymal cells, at least a part of the fragmented extracellular matrix component is placed between the first cells, and the epithelial structure contains epithelial cells.

公开(公告)号：US20220170911A1

公开(公告)日：2022-06-02

申请号：US17600348

申请日：2020-03-19

Applicant: TOPPAN INC. ,  OSAKA UNIVERSITY ,  Kyoto Prefectural Public University Corporation

Inventor： Shiro KITANO ,  Shinji IRIE ,  Michiya MATSUSAKI ,  Fiona LOUIS ,  Yoshihiro SOWA

IPC: G01N33/50 ,  C12N5/077 ,  C12N5/0775 ,  C12N5/071 ,  A61K35/35

Abstract: Disclosed is a cell structure comprising: a fragmented extracellular matrix component; and cells, wherein the cell structure comprises an intercellular vascular network, and the cells comprise at least adipocytes and vascular endothelial cells.

公开(公告)号：US20180355322A1

公开(公告)日：2018-12-13

申请号：US16104533

申请日：2018-08-17

Applicant: TOPPAN PRINTING CO., LTD. ,  OSAKA UNIVERSITY

Inventor： Kei TSUKAMOTO ,  Shinji IRIE ,  Michiya MATSUSAKI

IPC: C12N5/09

CPC classification number: C12N5/0693 ,  C12M3/00 ,  C12N2501/91

Abstract: A method of promoting spheroid formation, including: a preparation step of preparing a mixture obtained by mixing a cell sample with a promoter; and a culture step of culturing, inside a spheroid formation-culture vessel, the mixture obtained in the preparation step, in which the promoter is a polymer in which one or more selected from the group consisting of D-glucosamine, D-galactosamine, D-glucuronic acid, L-iduronic acid, and D-galactose are polymerized.

公开(公告)号：US20210063374A1

公开(公告)日：2021-03-04

申请号：US16644059

申请日：2018-09-03

Applicant: TOPPAN PRINTING CO., LTD. ,  OSAKA UNIVERSITY

Inventor： Michiya MATSUSAKI ,  Shiro KITANO ,  Shinji IRIE

IPC: G01N33/15 ,  C12N5/077 ,  C07K14/78

Abstract: The present invention relates to an abnormal cardiac rhythm myocardial model composed of a three-dimensional tissue containing cells including the cardiomyocytes and collagen, wherein at least a portion of the cells adheres to the collagen.

公开(公告)号：US20220133952A1

公开(公告)日：2022-05-05

申请号：US17502496

申请日：2021-10-15

Applicant: TOPPAN PRINTING CO., LTD. ,  OSAKA UNIVERSITY

Inventor： Michiya MATSUSAKI ,  Shinji IRIE ,  Shiro KITANO

IPC: A61L27/24 ,  A61L27/44 ,  A61L27/54 ,  A61L31/04 ,  C12N5/00 ,  A61L27/38

Abstract: A method may comprises bringing cells suspended in an aqueous medium into contact with a plurality of fragmented collagen pieces and, after the cells brought into contact with the plurality of fragmented collagen pieces and the plurality of fragmented collagen pieces are concentrated, culturing the cells brought into contact with the fragmented collagen pieces, with the plurality of fragmented collagen pieces, to form a three-dimensional tissue.

公开(公告)号：US20190351098A1

公开(公告)日：2019-11-21

申请号：US16480114

申请日：2018-01-31

Applicant: TOPPAN PRINTING CO., LTD. ,  OSAKA UNIVERSITY

Inventor： Michiya MATSUSAKI ,  Shinji IRIE ,  Shiro KITANO

IPC: A61L27/24 ,  C12N5/00 ,  A61L31/04 ,  A61L27/44 ,  A61L27/54

Abstract: Disclosed is a three-dimensional tissue comprising cells and collagen including endogenous collagen, wherein at least a portion of the cells is adhered to the collagen, and the content of the collagen is from 10 wt % to 90 wt % based on the three-dimensional tissue.

公开(公告)号：US20210115377A1

公开(公告)日：2021-04-22

申请号：US17050734

申请日：2019-05-07

Applicant: TOPPAN PRINTING CO., LTD. ,  OSAKA UNIVERSITY

Inventor： Shiro KITANO ,  Shinji IRIE ,  Michiya MATSUSAKI

IPC: C12M1/12 ,  D01F8/02

Abstract: The present invention relates to an extracellular-matrix-containing composition comprising: a fragmented extracellular matrix component; and an aqueous medium.