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    PROCESS FOR THE PRODUCTION OF BETA-LYSINE
    1.
    发明申请
    PROCESS FOR THE PRODUCTION OF BETA-LYSINE 审中-公开
    生产β-木质素的方法

    公开(公告)号：US20090029425A1

    公开(公告)日：2009-01-29

    申请号：US12281789

    申请日：2007-03-07

    申请人： Oskar Zelder ,  Weo Kyu Jeong ,  Corinna Klopprogge ,  Andrea Herold ,  Hartwig Schroder

    发明人： Oskar Zelder ,  Weo Kyu Jeong ,  Corinna Klopprogge ,  Andrea Herold ,  Hartwig Schroder

    IPC分类号： C12P13/08 ,  C12P13/00 ,  C12P17/10

    CPC分类号： C12P13/08 ,  C12P13/005 ,  C12P13/02

    摘要： Process for the production of -lysine by constructing a recombinant microorganism which has a deregulated lysine 2,3-aminomutase gene and at least one deregulated gene selected from the group (i) which consists of aspartokinase, aspartatesemialdehyde dehydrogenase, dihydrodipicolinate synthase, dihydrodipicolinate reductase, tetrahydrodipicolinate succinylase, succinyl-amino-ketopimelate transaminase, succinyl-diamino-pimelate desuccinylase, diaminopimelate epimerase, diamino-pimelate dehydrogenase, arginyl-tRNA synthetase, diaminopimelate decarboxylase, pyruvate carboxylase, phosphoenolpyruvate carboxylase, glucose-6-phosphate dehydrogenase, transketolase, transaldolase, 6-phosphogluconolactonase, fructose 1,6-biphosphatase, homoserine dehydrogenase, phophoenolpyruvate carboxykinase, succinyl-CoA synthetase, methylmalonyl-CoA mutase, provided that if aspartokinase is deregulated as gene (i) at least a second gene (i) other than aspartokinase has to be deregulated, and cultivating said microorganism.

    摘要翻译： 通过构建具有失调的赖氨酸2,3-氨基变位酶基因的重组微生物和选自由天冬氨酸激酶，天冬氨酸二甲二醇脱氢酶，二氢吡啶二羧酸合酶，二氢吡啶二羧酸还原酶，二氢吡啶二羧酸还原酶组成的组中的至少一种去调节基因的生产赖氨酸的方法， 四氢吡啶二羧酸琥珀酰基酶，琥珀酰 - 氨基 - 酮基庚酸酯转氨酶，琥珀酰 - 二氨基 - 庚二酸二琥珀酰化酶，二氨基庚酸酯差向异构酶，二氨基庚二酸脱氢酶，精氨酰-tRNA合成酶，二氨基庚二酸脱羧酶，丙酮酸羧化酶，磷酸烯醇丙酮酸羧化酶，葡萄糖-6-磷酸脱氢酶，转酮酶，转醛醇酶， 6-磷酸葡糖淀粉酶，果糖1,6-二磷酸酶，高丝氨酸脱氢酶，磷酸烯醇丙酮酸羧激酶，琥珀酰辅酶A合成酶，甲基丙二酰-CoA变位酶，条件是如果天冬氨酸激酶被解调为基因（i）至少第二种基因（i）除了天冬氨酸激酶 放松管制，培养说 微生物。

    Use of dimethyl disulfide for methionine production in microoragnisms
    2.
    发明申请
    Use of dimethyl disulfide for methionine production in microoragnisms 有权
    二甲基二硫化物用于微生物中的甲硫氨酸生产

    公开(公告)号：US20090281353A1

    公开(公告)日：2009-11-12

    申请号：US11988971

    申请日：2006-07-18

    申请人： Oskar Zelder ,  Stefan Haefner ,  Andrea Herold ,  Corinna Klopprogge ,  Hartwig Schroder ,  R. Rogers Yocum ,  Mark K. Williams

    发明人： Oskar Zelder ,  Stefan Haefner ,  Andrea Herold ,  Corinna Klopprogge ,  Hartwig Schroder ,  R. Rogers Yocum ,  Mark K. Williams

    IPC分类号： C12P13/12 ,  C07C323/03 ,  C12N1/21 ,  C12N15/31 ,  C12Q1/68 ,  C12N9/00

    CPC分类号： C12P13/12

    摘要： The present invention features improved processes and organisms for the production of methionine. The invention demonstrates that a ΔmetF organism or a ΔmetE AmetH organism, for example, mutants of C. glutamicum or E. coli, can use a methyl capped sulfide source, e.g., dimethyl disulfide (DMDS), as a source of both sulfur and a methyl group, bypassing the need for MetH/MetE and MetF activity and the need to reduce sulfate, for the synthesis of methionine. Also described in this patent are data implicating MetY (also called MetZ) as an enzyme that incorporates a methyl capped sulfide source, e.g., DMDS, into methionine. A ΔmetF ΔmetB strain of C. glutamicum can use a methyl capped sulfide source, e.g., DMDS, as a source of both sulfide and a methyl group. Furthermore, methionine production by engineered prototrophic organisms that overproduce O-acetyl-homoserine was improved by the addition of a methyl capped sulfide source, e.g., DMDS.

    摘要翻译： 本发明的特征在于用于生产甲硫氨酸的改进方法和生物体。 本发明证明，DeltametF生物体或DeltametE AmetH生物体，例如谷氨酸棒状杆菌或大肠杆菌的突变体可以使用甲基封端的硫化物源，例如二甲基二硫醚（DMDS）作为硫和 甲基，绕过MetH / MetE和MetF活性的需要，需要还原硫酸盐，用于合成甲硫氨酸。 该专利中还描述的是涉及MetY（也称为MetZ）作为将甲基封端的硫化物源例如DMDS掺入甲硫氨酸的酶的数据。 谷氨酸棒状病毒的DeltametF DeltametB菌株可以使用甲基封端的硫化物源，例如DMDS，作为硫化物和甲基两者的来源。 此外，通过加入甲基封端的硫化物源（例如DMDS），改善过量产生O-乙酰高丝氨酸的工程化原养殖生物体的甲硫氨酸生产。

    Use of dimethyl disulfide for methionine production in microoraganisms
    4.
    发明授权
    Use of dimethyl disulfide for methionine production in microoraganisms 有权
    用二甲基二硫化物在微生物激素中生产甲硫氨酸

    公开(公告)号：US08399214B2

    公开(公告)日：2013-03-19

    申请号：US11988971

    申请日：2006-07-18

    申请人： Oskar Zelder ,  Stefan Haefner ,  Andrea Herold ,  Corinna Klopprogge ,  Hartwig Schroder ,  R. Rogers Yocum ,  Mark K. Williams

    发明人： Oskar Zelder ,  Stefan Haefner ,  Andrea Herold ,  Corinna Klopprogge ,  Hartwig Schroder ,  R. Rogers Yocum ,  Mark K. Williams

    IPC分类号： C12P1/00

    CPC分类号： C12P13/12

    摘要： The present invention features improved processes and organisms for the production of methionine. The invention demonstrates that a ΔmetF organism or a ΔmetE AmetH organism, for example, mutants of C. glutamicum or E. coli, can use a methyl capped sulfide source, e.g., dimethyl disulfide (DMDS), as a source of both sulfur and a methyl group, bypassing the need for MetH/MetE and MetF activity and the need to reduce sulfate, for the synthesis of methionine. Also described in this patent are data implicating MetY (also called MetZ) as an enzyme that incorporates a methyl capped sulfide source, e.g., DMDS, into methionine. A ΔmetF ΔmetB strain of C. glutamicum can use a methyl capped sulfide source, e.g., DMDS, as a source of both sulfide and a methyl group. Furthermore, methionine production by engineered prototrophic organisms that overproduce O-acetyl-homoserine was improved by the addition of a methyl capped sulfide source, e.g., DMDS.

    摘要翻译： 本发明的特征在于用于生产甲硫氨酸的改进方法和生物体。 本发明证明，一种有机蛋白（MetF）生物体或一种具有谷氨酸棒状杆菌或大肠杆菌的突变体的MetE生物体可以使用例如二甲基二硫醚（DMDS）的甲基封端硫化物源作为 硫和甲基，绕过MetH / MetE和MetF活性的需要，需要还原硫酸盐，用于合成甲硫氨酸。 该专利中还描述的是涉及MetY（也称为MetZ）作为将甲基封端的硫化物源例如DMDS掺入甲硫氨酸的酶的数据。 A＆Dgr; metF＆Dgr;谷氨酸棒杆菌的metB菌株可以使用甲基封端的硫化物源，例如DMDS，作为硫化物和甲基的来源。 此外，通过加入甲基封端的硫化物源（例如DMDS），改善过量产生O-乙酰高丝氨酸的工程化原养殖生物体的甲硫氨酸生产。

    Multiple promoters and the use thereof for gene expression
    8.
    发明授权
    Multiple promoters and the use thereof for gene expression 有权
    多重启动子及其用于基因表达的用途

    公开(公告)号：US07968699B2

    公开(公告)日：2011-06-28

    申请号：US12048599

    申请日：2008-03-14

    申请人： Stefan Haefner ,  Hartwig Schroder ,  Oskar Zelder ,  Corinna Klopprogge ,  Andrea Herold

    发明人： Stefan Haefner ,  Hartwig Schroder ,  Oskar Zelder ,  Corinna Klopprogge ,  Andrea Herold

    IPC分类号： C07H21/04 ,  C12N15/85

    CPC分类号： C07K14/34 ,  C12N15/77

    摘要： The present invention relates to multiple promoters and to expression units comprising them; to the use thereof for regulating transcription and expression of genes; to expression cassettes which comprise multiple promoters or expression units of this kind; to vectors which comprise such expression cassettes; to genetically modified microorganisms which comprise vectors and/or expression units of this kind; and to processes for preparing biosynthetic products by culturing said genetically modified microorganisms.

    摘要翻译： 本发明涉及多种启动子和包含它们的表达单元; 用于调节基因的转录和表达; 包括多种启动子或这种表达单位的表达盒; 包含这种表达盒的载体; 涉及包含这种载体和/或表达单元的转基因微生物; 以及通过培养所述转基因微生物来制备生物合成产物的方法。

    Psod Expression Units
    9.
    发明申请
    Psod Expression Units 审中-公开
    Psod表达单位

    公开(公告)号：US20080274516A1

    公开(公告)日：2008-11-06

    申请号：US10583191

    申请日：2004-12-16

    申请人： Burkhard Kroger ,  Oskar Zelder ,  Corinna Klopprogge ,  Hartwig Schroder ,  Stefan Haefner

    发明人： Burkhard Kroger ,  Oskar Zelder ,  Corinna Klopprogge ,  Hartwig Schroder ,  Stefan Haefner

    IPC分类号： C12N15/77 ,  C12N15/09 ,  C12N15/31 ,  C12P13/08 ,  C12P13/12

    CPC分类号： C12N15/77

    摘要： The present invention relates to the use of nucleic acid sequences for regulating the transcription and expression of genes, the novel promoters and expression units themselves, methods for altering or causing the transcription rate and/or expression rate of genes, expression cassettes comprising the expression units, genetically modified microorganisms with altered or caused transcription rate and/or expression rate, and methods for preparing biosynthetic products by cultivating the genetically modified microorganisms.

    摘要翻译： 本发明涉及核酸序列用于调节基因的转录和表达，新型启动子和表达单元本身的用途，用于改变或引起基因的转录速率和/或表达速率的方法，包含表达单位的表达盒 具有改变或引起的转录速率和/或表达速率的转基因微生物，以及通过培养转基因微生物来制备生物合成产物的方法。