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22 条记录 (耗时 0.055 秒)

    Microorganisms and processes for enhanced production of pantothenate
    6.
    发明授权
    Microorganisms and processes for enhanced production of pantothenate 有权
    用于增加泛酸盐生产的微生物和方法

    公开(公告)号：US07291489B2

    公开(公告)日：2007-11-06

    申请号：US10614333

    申请日：2003-07-03

    申请人： R. Rogers Yocum ,  Thomas A. Patterson ,  Janice G. Pero ,  Theron Hermann

    发明人： R. Rogers Yocum ,  Thomas A. Patterson ,  Janice G. Pero ,  Theron Hermann

    IPC分类号： C12P13/04 ,  C12N9/00 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C12N15/52 ,  C12P13/02

    摘要： The present invention features improved methods for the enhanced production of pantoate and pantothenate utilizing microorganisms having modified pantothenate biosynthetic enzyme activities and having modified methylenetetrahydrofolate (MTF) biosynthetic enzyme activities. In particular, the invention features methods for enhancing production of desired products by increasing levels of a key intermediate, ketopantoate, by increasing enzymes or substrates that contribute directly or indirectly to its synthesis. Recombinant microorganisms and conditions for culturing same are also are featured. Also featured are compositions produced by such microorganisms.

    摘要翻译： 本发明的特征在于利用具有改良的泛酸生物合成酶活性并具有改性的亚甲基四氢叶酸（MTF）生物合成酶活性的微生物增强生产泛酸盐和泛酸盐的改进方法。 特别地，本发明的特征在于通过增加对其合成直接或间接有贡献的酶或底物来增加关键中间体酮托品酸的水平来增强所需产物的产生的方法。 还具有重组微生物和培养条件。 还有这些微生物产生的组合物。

    Methods and microorganisms for production of panto-compounds
    8.
    发明授权
    Methods and microorganisms for production of panto-compounds 有权
    泛化合物生产方法和微生物

    公开(公告)号：US08232081B2

    公开(公告)日：2012-07-31

    申请号：US10984449

    申请日：2004-11-08

    申请人： R. Rogers Yocum ,  Thomas A. Patterson ,  Theron Hermann ,  Janice G. Pero

    发明人： R. Rogers Yocum ,  Thomas A. Patterson ,  Theron Hermann ,  Janice G. Pero

    IPC分类号： C12P13/04 ,  C12P13/02 ,  C12P7/42 ,  C12P7/18

    CPC分类号： C12P17/04 ,  C12N15/52 ,  C12P13/02

    摘要： The present invention features methods of producing panto-compounds (e.g., pantothenate) using microorganisms in which the pantothenate biosynthetic pathway and/or the isoleucine-valine biosynthetic pathway and/or the coenzymeA biosynthetic pathway has been manipulated. Methods featuring ketopantoate reductase overexpressing microorganisms as well as aspartate α-decarboxylase overexpressing microorganisms are provided. Methods of producing panto-compounds in a precursor-independent manner and in high yield are described. Recombinant microorganisms, vectors, isolated nucleic acid molecules, genes and gene products useful in practicing the above methodologies are also provided. The present invention also features a previously unidentified microbial pantothenate kinase gene, coaX, as well as methods of producing panto-compounds utilizing microorganisms having modified pantothenate kinase activity. Recombinant microorganisms, vectors, isolated coaX nucleic acid molecules and purified CoaX proteins are featured. Also featured are methods for identifying pantothenate kinase modulators utilizing the recombinant microorganisms and/or purified CoaX proteins of the present invention.

    摘要翻译： 本发明的特征在于使用其中泛酸生物合成途径和/或异亮氨酸 - 缬氨酸生物合成途径和/或辅酶A生物合成途径已经被操纵的微生物来生产泛化合物（例如泛酸盐）的方法。 提供了酮过氧还原酶过表达微生物以及天冬氨酸α-脱羧酶过表达微生物的方法。 描述了以前体非依赖性方式和高产率生产泛化合物的方法。 还提供了用于实践上述方法的重组微生物，载体，分离的核酸分子，基因和基因产物。 本发明还具有以前未鉴定的微生物泛酸激酶基因coaX，以及利用具有改良的泛酸激酶活性的微生物产生泛化合物的方法。 重组微生物，载体，分离的coaX核酸分子和纯化的CoaX蛋白。 还特征在于利用本发明的重组微生物和/或纯化的CoaX蛋白鉴定泛酸激酶调节剂的方法。

    Engineering microbes for efficient production of chemicals
    9.
    发明授权
    Engineering microbes for efficient production of chemicals 有权
    用于高效生产化学品的工程微生物

    公开(公告)号：US09017976B2

    公开(公告)日：2015-04-28

    申请号：US13503999

    申请日：2010-11-17

    申请人： Wei Gong ,  Sudhanshu Dole ,  Tammy Grabar ,  Andrew Christopher Collard ,  Janice G. Pero ,  R. Rogers Yocum

    发明人： Wei Gong ,  Sudhanshu Dole ,  Tammy Grabar ,  Andrew Christopher Collard ,  Janice G. Pero ,  R. Rogers Yocum

    IPC分类号： B29C70/04 ,  C12N15/74 ,  C12N1/00 ,  A61B6/04 ,  B29L31/00

    CPC分类号： A61B6/0442 ,  B29C70/04 ,  B29L2031/753 ,  C12N9/1205 ,  C12N9/88 ,  C12N15/52 ,  C12P7/40 ,  C12P7/46

    摘要： This present invention relates to production of chemicals from microorganisms that have been genetically engineered and metabolically evolved. Improvements in chemical production have been established, and particular mutations that lead to those improvements have been identified. Specific examples are given in the identification of mutations that occurred during the metabolic evolution of a bacterial strain genetically engineered to produce succinic acid. This present invention also provides a method for evaluating the industrial applicability of mutations that were selected during the metabolic evolution for increased succinic acid production. This present invention further provides microorganisms engineered to have mutations that are selected during metabolic evolution and contribute to improved production of succinic acid, other organic acids and other chemicals of commercial interest.

    摘要翻译： 本发明涉及从遗传工程和代谢进化的微生物生产化学物质。 已经建立了化学生产的改进，并确定了导致这些改进的特定突变。 在鉴定在遗传工程化生产琥珀酸的细菌菌株的代谢进化期间发生的突变的具体实例。 本发明还提供了用于评估在增加的琥珀酸生产的代谢进化期间选择的突变的工业适用性的方法。 本发明进一步提供了工程改造成具有在代谢进化期间选择的突变的微生物，并有助于改善琥珀酸，其它有机酸和其它商业利益的化学品的生产。