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    SINGLE USE SLURRYING AND CHROMATOGRAPHY SYSTEMS
    3.
    发明申请
    SINGLE USE SLURRYING AND CHROMATOGRAPHY SYSTEMS 审中-公开
    单一使用浆料和色谱系统

    公开(公告)号：US20130186834A1

    公开(公告)日：2013-07-25

    申请号：US13825829

    申请日：2011-09-23

    申请人： John J. Vicalvi ,  Joseph Makowiecki ,  Joseph D. Crowell ,  Parrish M. Galliher

    发明人： John J. Vicalvi ,  Joseph Makowiecki ,  Joseph D. Crowell ,  Parrish M. Galliher

    IPC分类号： B01D15/22

    CPC分类号： B01D15/22 ,  B01F7/00141 ,  B01F7/00858 ,  B01F7/161 ,  B01F7/162 ,  B01F7/1695 ,  B01F7/22 ,  B01F13/0211 ,  B01F13/065 ,  B01F13/0827 ,  B01F15/00162 ,  B01F15/00175 ,  B01F15/0022 ,  B01F15/00227 ,  B01F15/0085 ,  B01F15/065 ,  G01N30/50 ,  G01N30/56 ,  G01N30/6017 ,  G01N30/603 ,  G01N2030/565

    摘要： Methods and systems for chromatography are disclosed that employ a flexible container configured to fit within a support structure and adapted to receive a filtration or absorptive medium, such as a chromatography resin. The flexible container can include at least one inlet, at least one outlet, and a separation barrier peripherally sealed within the container to separate the container into a resin containing portion and a drainage portion. The barrier can be configured to exclude the resin material from the drainage portion during use while allowing fluids to pass therethrough. The disposable chromatography system can further include one or more agitators disposed within the flexible container and adjustably configured to be raised or lowered in the flexible container. When the agitator is in the raised position, the resin packing material can operate in a settled, packed-bed configuration. Alternatively, the agitator in the lowered position permits the chromatography resin packing material to operate in a mixed, slurry configuration.

    摘要翻译： 公开了用于层析的方法和系统，其采用配置成装配在支撑结构内并适于接收过滤或吸收介质如色谱树脂的柔性容器。 柔性容器可以包括至少一个入口，至少一个出口和周边地密封在容器内的分离屏障，以将容器分离成树脂容纳部分和排出部分。 挡板可以构造成在使用期间将树脂材料从排水部分排除，同时允许流体通过。 一次性色谱系统还可以包括设置在柔性容器内的一个或多个搅拌器，并可调节地构造成在柔性容器中升高或降低。 当搅拌器处于升起位置时，树脂包装材料可以以沉降的填充床构型运行。 或者，处于降低位置的搅拌器允许色谱树脂包装材料以混合的浆料配置运行。

    DRAIN DOWN AND RE-FEED OF MICROCARRIER BIOREACTOR
    5.
    发明申请
    DRAIN DOWN AND RE-FEED OF MICROCARRIER BIOREACTOR 审中-公开
    微生物生物反应器的排水和再输送

    公开(公告)号：US20120190068A1

    公开(公告)日：2012-07-26

    申请号：US13355125

    申请日：2012-01-20

    申请人： Patrick M. GUERTIN ,  Joseph D. Crowell

    发明人： Patrick M. GUERTIN ,  Joseph D. Crowell

    IPC分类号： C12N7/02 ,  C12P21/00

    CPC分类号： C12N7/00 ,  C12M25/14 ,  C12M47/10 ,  C12N2770/24151

    摘要： Disclosed is a method of increasing product yield per culture in a population of bound product-secreting cells in a bioreactor, the method comprising: semi-harvesting product by removing a volume of the culture medium with a first-secreted product concentration; re-feeding the bound population of product-secreting cells by adding an amount of a fresh culture medium sufficient to increase the volume of the culture medium to approximately the original volume of the culture medium; agitating the culture medium under sufficient conditions and for a sufficient time period to allow the bound population of product-secreting cells to grow and to release a second-secreted product concentration into the culture medium; and harvesting product.

    摘要翻译： 公开了一种提高生物反应器中结合产物分泌细胞群体中每种培养物的产量的方法，该方法包括：通过以第一分泌产物浓度去除体积的培养基来半成品; 通过添加足量的培养基，将培养基的体积增加至大约原始体积的培养基，再次喂养结合的产物分泌细胞群; 在足够的条件下搅拌培养基足够的时间以允许结合的产物分泌细胞群体生长并将第二分泌的产物浓度释放到培养基中; 并收获产品。