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    Tandem Purification of Proteins
    6.
    发明申请
    Tandem Purification of Proteins 审中-公开
    蛋白质的串联纯化

    公开(公告)号：US20130317198A1

    公开(公告)日：2013-11-28

    申请号：US13811178

    申请日：2011-07-29

    申请人： Jonathan Lee Coffman ,  Ranganathan Godavarti ,  Michael Shamashkin

    发明人： Jonathan Lee Coffman ,  Ranganathan Godavarti ,  Michael Shamashkin

    IPC分类号： C07K1/16 ,  C07K16/00

    CPC分类号： C07K1/165 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/00

    摘要： The present disclosure provides methods for purifying products from a fluid. In some embodiments, provided purification methods use a combination of purification modes (e.g., protein A and ion exchange) operated in tandem, wherein at least one of the modes utilizes weak partitioning. In some embodiments, provided purification methods operate under robust conditions in which a degree of binding between a product and resin is maintained despite variations in operating parameters.

    摘要翻译： 本公开提供了从流体中纯化产物的方法。 在一些实施方案中，提供的纯化方法使用串联操作的纯化模式（例如蛋白A和离子交换）的组合，其中至少一种模式利用弱分配。 在一些实施方案中，提供的纯化方法在稳定的条件下操作，其中尽管操作参数有变化，但仍保持产品和树脂之间的结合程度。

    Tandem purification of proteins
    7.
    发明授权
    Tandem purification of proteins 有权
    蛋白质的串联纯化

    公开(公告)号：US09428546B2

    公开(公告)日：2016-08-30

    申请号：US13811178

    申请日：2011-07-29

    申请人： Jonathan Lee Coffman ,  Ranganathan Godavarti ,  Michael Shamashkin

    发明人： Jonathan Lee Coffman ,  Ranganathan Godavarti ,  Michael Shamashkin

    IPC分类号： C07K1/16 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/00

    CPC分类号： C07K1/165 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/00

    摘要： The present disclosure provides methods for purifying products from a fluid. In some embodiments, provided purification methods use a combination of purification modes (e.g., protein A and ion exchange) operated in tandem, wherein at least one of the modes utilizes weak partitioning. In some embodiments, provided purification methods operate under robust conditions in which a degree of binding between a product and resin is maintained despite variations in operating parameters.

    摘要翻译： 本公开提供了从流体中纯化产物的方法。 在一些实施方案中，提供的纯化方法使用串联操作的纯化模式（例如蛋白A和离子交换）的组合，其中至少一种模式利用弱分配。 在一些实施方案中，提供的纯化方法在稳定的条件下操作，其中尽管操作参数有变化，但仍保持产品和树脂之间的结合程度。

    Methods of purifying anti a beta antibodies
    8.
    发明申请
    Methods of purifying anti a beta antibodies 有权
    纯化抗β抗体的方法

    公开(公告)号：US20070082367A1

    公开(公告)日：2007-04-12

    申请号：US11454772

    申请日：2006-06-16

    申请人： Ranganathan Godavarti ,  Timothy Iskra

    发明人： Ranganathan Godavarti ,  Timothy Iskra

    IPC分类号： G01N33/567 ,  C07H21/04 ,  C12P21/06 ,  C12N5/06

    CPC分类号： C07K16/065 ,  C07K16/18 ,  G01N33/6854

    摘要： The present application provides methods of purifying Aβ binding proteins having a Fc region, for example, anti-Aβ antibodies or antibody fusions, by adsorbing the Aβ binding protein to a Fc binding agent, such as, for example, Protein A or Protein G, followed by a wash with a divalent cation salt buffer to remove impurities and subsequent recovery of the adsorbed Aβ binding protein. The present application also features methods of eluting the purified Aβ binding protein as well as the incorporation of the methods within a purification train. Kits comprising components for carrying out the methods and instructions for use are also provided.

    摘要翻译： 本申请提供了通过将Abeta结合蛋白吸附到Fc结合剂（例如蛋白A或蛋白G）上来纯化具有Fc区的Abeta结合蛋白，例如抗Aβ抗体或抗体融合物的方法， 然后用二价阳离子盐缓冲液洗涤以除去杂质并随后回收吸附的Abeta结合蛋白。 本申请还具有洗脱纯化的Abeta结合蛋白的方法以及将该方法并入纯化列中。 还提供了包括用于执行使用方法和使用说明的组件的组件。

    Methods of purifying anti A β antibodies
    9.
    发明授权
    Methods of purifying anti A β antibodies 有权
    纯化抗Aβ抗体的方法

    公开(公告)号：US08440799B2

    公开(公告)日：2013-05-14

    申请号：US12903053

    申请日：2010-10-12

    申请人： Ranganathan Godavarti ,  Timothy Iskra

    发明人： Ranganathan Godavarti ,  Timothy Iskra

    IPC分类号： C07K16/00 ,  C07K1/00

    CPC分类号： C07K16/065 ,  C07K16/18 ,  G01N33/6854

    摘要： The present application provides methods of purifying Aβ binding proteins having a Fc region, for example, anti-Aβ antibodies or antibody fusions, by adsorbing the Aβ binding protein to a Fc binding agent, such as, for example, Protein A or Protein G, followed by a wash with a divalent cation salt buffer to remove impurities and subsequent recovery of the adsorbed Aβ binding protein. The present application also features methods of eluting the purified Aβ binding protein as well as the incorporation of the methods within a purification train. Kits comprising components for carrying out the methods and instructions for use are also provided.

    摘要翻译： 本申请提供了通过将Abeta结合蛋白吸附到Fc结合剂（例如蛋白A或蛋白G）上来纯化具有Fc区的Abeta结合蛋白，例如抗Aβ抗体或抗体融合物的方法， 然后用二价阳离子盐缓冲液洗涤以除去杂质并随后回收吸附的Abeta结合蛋白。 本申请还具有洗脱纯化的Abeta结合蛋白的方法以及将该方法并入纯化列中。 还提供了包括用于执行使用方法和使用说明的组件的组件。

    Methods of purifying anti A β antibodies
    10.
    发明授权
    Methods of purifying anti A β antibodies 有权
    抗A＆Bgr的纯化方法 抗体

    公开(公告)号：US07825223B2

    公开(公告)日：2010-11-02

    申请号：US11454772

    申请日：2006-06-16

    申请人： Ranganathan Godavarti ,  Timothy Iskra

    发明人： Ranganathan Godavarti ,  Timothy Iskra

    IPC分类号： C07K16/18 ,  C07K1/22

    CPC分类号： C07K16/065 ,  C07K16/18 ,  G01N33/6854

    摘要： The present application provides methods of purifying Aβ binding proteins having a Fc region, for example, anti-Aβ antibodies or antibody fusions, by adsorbing the Aβ binding protein to a Fc binding agent, such as, for example, Protein A or Protein G, followed by a wash with a divalent cation salt buffer to remove impurities and subsequent recovery of the adsorbed Aβ binding protein. The present application also features methods of eluting the purified Aβ binding protein as well as the incorporation of the methods within a purification train. Kits comprising components for carrying out the methods and instructions for use are also provided.

    摘要翻译： 本申请提供了A＆bgr的纯化方法; 具有Fc区的结合蛋白，例如抗A＆bgr; 抗体或抗体融合物，通过吸附A＆bgr; 结合蛋白与Fc结合剂，例如蛋白A或蛋白G，然后用二价阳离子盐缓冲液洗涤以除去杂质并随后回收吸附的A和Bgr; 结合蛋白。 本申请还具有洗脱纯化的A＆bgr的方法; 结合蛋白以及将该方法并入纯化列中。 还提供了包括用于执行使用方法和使用说明的组件的组件。