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    Analyte injection system
    9.
    发明申请
    Analyte injection system 审中-公开
    分析物注射系统

    公开(公告)号：US20050133370A1

    公开(公告)日：2005-06-23

    申请号：US10865959

    申请日：2004-06-12

    申请人： Charles Park ,  Persefoni Kechagia ,  Michael Spaid ,  Morten Jensen ,  Irina Kazakova ,  Josh Molho ,  Tomohisa Kawabata ,  Mitsuo Watanabe

    发明人： Charles Park ,  Persefoni Kechagia ,  Michael Spaid ,  Morten Jensen ,  Irina Kazakova ,  Josh Molho ,  Tomohisa Kawabata ,  Mitsuo Watanabe

    IPC分类号： B01L3/00 ,  C12Q1/68 ,  G01N27/447 ,  G01N27/453

    CPC分类号： G01N27/44791 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0673 ,  B01L2400/0415 ,  C12Q1/6804 ,  C12Q1/6883 ,  G01N27/44773 ,  C12Q2565/629 ,  C12Q2565/125 ,  C12Q2563/179

    摘要： This invention provides methods and devices for spatially separating at least first and second components in a sample which in one exemplary embodiment comprises introducing the first and second components into a first microfluidic channel of a microfluidic device in a carrier fluid comprising a spacer electrolyte solution and stacking the first and second components by isotachophoresis between a leading electrolyte solution and a trailing electrolyte solution, wherein the spacer electrolyte solution comprises ions which have an intermediate mobility in an electric field between the mobility of the ions present in the leading and trailing electrolyte solutions and wherein the spacer electrolyte solution comprises at least one of the following spacer ions MOPS, MES, Nonanoic acid, D-Glucuronic acid, Acetylsalicyclic acid, 4-Ethoxybenzoic acid, Glutaric acid, 3-Phenylpropionic acid, Phenoxyacetic acid, Cysteine, hippuric acid, p-hydroxyphenylacetic acid, isopropylmalonic acid, itaconic acid, citraconic acid, 3,5-dimethylbenzoic acid, 2,3-dimethylbenzoic acid, p-hydroxycinnamic acid, and 5-br-2,4-dihydroxybenzoic acid, and wherein the first component comprises a DNA-antibody conjugate and the second component comprises a complex of the DNA-antibody conjugate and an analyte.

    摘要翻译： 本发明提供了用于空间分离样品中的至少第一和第二组分的方法和装置，其在一个示例性实施方案中包括将第一和第二组分引入载体流体中的微流体装置的第一微流体通道，所述载体流体包括间隔电解质溶液和层叠 所述第一和第二组分通过在前导电解质溶液和尾部电解质溶液之间的等速电泳，其中所述间隔电解质溶液包含在电场中具有中间迁移率的离子，所述离子存在于所述前导和后续电解质溶​​液中的离子的迁移率之间， 间隔电解质溶液包含以下间隔离子MOPS，MES，壬酸，D-葡糖醛酸，乙酰水杨酸，4-乙氧基苯甲酸，戊二酸，3-苯基丙酸，苯氧基乙酸，半胱氨酸，马尿酸，p 羟基苯乙酸，异丙基丙二酸，衣康酸a cid，柠康酸，3,5-二甲基苯甲酸，2,3-二甲基苯甲酸，对羟基肉桂酸和5-溴-2,4-二羟基苯甲酸，其中第一组分包含DNA-抗体缀合物，第二组分包含第二 组分包含DNA抗体缀合物和分析物的复合物。