Integration of direct binding label-free biosensors with mass spectrometry for functional and structural characterization of molecules
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    发明申请
    Integration of direct binding label-free biosensors with mass spectrometry for functional and structural characterization of molecules 审中-公开
    将直接结合无标记的生物传感器与质谱法结合,用于分子的功能和结构表征

    公开(公告)号:US20060003372A1

    公开(公告)日:2006-01-05

    申请号:US11168155

    申请日:2005-06-28

    IPC分类号: C12Q1/68 G01N33/53

    摘要: The invention provides methods for the detection, quantification, identification and structural analysis of one or more molecules. Mass spectrometry (MS) is not a universal detector as all molecules do not ionize equally well leading to poor signal to quantity information. MS can be optimized to identify the specific mass of a binding component when the presence of a material is known. Colorimetric resonant reflectance optical sensors provide a universal mass detector in that nearly all biological masses give equally proportional signals. The combined methods allow selection and or detection with quantification of all masses binding to the sensor with the ability to identify specific molecules by their individual masses and structure analyses.

    摘要翻译: 本发明提供了一种或多种分子的检测,定量,鉴定和结构分析的方法。 质谱(MS)不是一个通用的检测器,因为所有的分子都不会很好地电离,导致信号对数量信息的差。 当材料的存在已知时,可优化MS以鉴定结合组分的特定质量。 比色共振反射光学传感器提供了通用的质量检测器,因为几乎所有的生物质量都产生等比例的信号。 组合的方法允许通过定量结合传感器的所有质量的选择和/或检测,其具有通过其各自的质量和结构分析鉴定特定分子的能力。