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    Process for the fermentative preparation of L-amino acids using coryneform bacteria
    1.
    发明授权
    Process for the fermentative preparation of L-amino acids using coryneform bacteria 失效
    使用棒状细菌发酵制备L-氨基酸的方法

    公开(公告)号：US06596516B2

    公开(公告)日：2003-07-22

    申请号：US09731826

    申请日：2000-12-08

    申请人： Petra Ziegler ,  Lothar Eggeling ,  Hermann Sahm ,  Georg Thierbach ,  Walter Pfefferle

    发明人： Petra Ziegler ,  Lothar Eggeling ,  Hermann Sahm ,  Georg Thierbach ,  Walter Pfefferle

    IPC分类号： C12P1304

    CPC分类号： C12N9/1014 ,  C12P13/04 ,  C12P13/08

    摘要： A process for the preparation of L-amino acids, in which the following steps are carried out, a) fermenting the desired L-amino acid-producing bacteria in which at least the glyA gene is attenuated, in particular by removal of the natural promoter, and optionally b) concentrating the desired product in the medium or in the cells of the bacteria and c) isolating the L-amino acid, and optionally bacteria in which further genes of the biosynthesis pathway of the desired L-amino acid are additionally amplified are employed, or bacteria in which the metabolic pathways which reduce the formation of the desired L-amino acid are at least partly eliminated are employed, and nucleotide sequences of the lacI-tac-5′glyA or lacI-tac-glyA unit.

    摘要翻译： 一种制备L-氨基酸的方法，其中进行以下步骤：a）将至少所述glyA基因减毒的所需L-氨基酸产生细菌发酵，特别是通过除去天然启动子 并且任选地b）将所需产物浓缩在细菌的培养基中或细胞中，并且c）分离L-氨基酸，以及任选的细菌，其中另外扩增所需L-氨基酸的生物合成途径的其它基因的细菌 或其中代谢途径减少所需L-氨基酸的形成至少部分消除的细菌，以及lacI-tac-5'glyA或lacI-tac-glyA单元的核苷酸序列。

    Nucleotide sequences which code for the ACP gene
    10.
    发明授权
    Nucleotide sequences which code for the ACP gene 失效
    编码ACP基因的核苷酸序列

    公开(公告)号：US06830921B2

    公开(公告)日：2004-12-14

    申请号：US09852137

    申请日：2001-05-10

    申请人： Bettina Möckel ,  Anke Weissenborn ,  Walter Pfefferle ,  Hermann Sahm ,  Lothar Eggeling ,  Madhavan Nampoothiri

    发明人： Bettina Möckel ,  Anke Weissenborn ,  Walter Pfefferle ,  Hermann Sahm ,  Lothar Eggeling ,  Madhavan Nampoothiri

    IPC分类号： C12N120

    CPC分类号： C12P13/08 ,  C07K14/34

    摘要： The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70 % to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70 % to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), a process for the fermentative preparation of L-amino acids with enhancement of the acp gene and the use of the polynucleotide as a primer or hybridization probe.

    摘要翻译： 本发明涉及一种分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸的程度相同的多核苷酸 b）编码多肽的多核苷酸，其包含与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列，c）与a）的多核苷酸互补的多核苷酸， 或b），和d）多核苷酸，其包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸，具有增强的acp基因的L-氨基酸的发酵制备方法和所述多核苷酸的用途 作为引物或杂交探针。