公开(公告)号：US06610663B2

公开(公告)日：2003-08-26

申请号：US09974326

申请日：2001-10-10

申请人： Phillip Dan Cook ,  Thomas Bruice ,  Charles John Guinosso ,  Andrew Mamoru Kawasaki ,  Richard Griffey

发明人： Phillip Dan Cook ,  Thomas Bruice ,  Charles John Guinosso ,  Andrew Mamoru Kawasaki ,  Richard Griffey

IPC分类号： A61K4800

CPC分类号： C12N15/113 ,  A61K38/00 ,  A61K48/00 ,  C07H19/04 ,  C07H19/06 ,  C07H19/10 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  C07H23/00 ,  C07J43/003 ,  C07K14/005 ,  C12N9/0069 ,  C12N15/1137 ,  C12N2310/3125 ,  C12N2310/315 ,  C12N2310/32 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/33 ,  C12N2310/333 ,  C12N2310/336 ,  C12N2310/3511 ,  C12N2310/3521 ,  C12N2310/3523 ,  C12N2310/3527 ,  C12N2310/3531 ,  C12N2310/3533 ,  C12N2740/16322 ,  C12Q1/68 ,  C12Q1/6813 ,  C12Y113/11012

摘要： Compositions and methods for modulating the activity of RNA are disclosed. In accordance with preferred embodiments, antisense compositions are prepared comprising targeting and reactive portions. The reactive portions preferably comprise one or two imidazole functionalities conjugated to the targeting oligonucleotide via linkers with or without intervening intercalating moieties. Therapeutics, diagnostics and research methods also are disclosed, as are synthetic nucleosides and nucleoside fragments that can be elaborated into oligonucleotides.

公开(公告)号：US06358931B1

公开(公告)日：2002-03-19

申请号：US08295744

申请日：1994-08-30

申请人： Phillip Dan Cook ,  Thomas Bruice ,  Charles John Guinosso ,  Andrew Mamoru Kawasaki ,  Richard Griffey

发明人： Phillip Dan Cook ,  Thomas Bruice ,  Charles John Guinosso ,  Andrew Mamoru Kawasaki ,  Richard Griffey

IPC分类号： C07H2107

CPC分类号： C12N15/113 ,  A61K38/00 ,  A61K48/00 ,  C07H19/04 ,  C07H19/06 ,  C07H19/10 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  C07H23/00 ,  C07J43/003 ,  C07K14/005 ,  C12N9/0069 ,  C12N15/1137 ,  C12N2310/3125 ,  C12N2310/315 ,  C12N2310/32 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/33 ,  C12N2310/333 ,  C12N2310/336 ,  C12N2310/3511 ,  C12N2310/3521 ,  C12N2310/3523 ,  C12N2310/3527 ,  C12N2310/3531 ,  C12N2310/3533 ,  C12N2740/16322 ,  C12Q1/68 ,  C12Q1/6813 ,  C12Y113/11012

摘要： Compositions and methods for modulating the activity of RNA are disclosed, In accordance with preferred embodiments, antisense compositions are prepared targeting reactive portions. The reactive portions preferably comprise one or two imidazole functionalities conjugated to the targeting oligonucleotide via linkers with or without intervening intercalating moieties. Therapeutics, diagnostics and research methods also are disclosed, as are synthetic nucleosides and nucleoside fragments that can be elaborated into oligonucleotides.

摘要翻译： 公开了调节RNA活性的组合物和方法。根据优选实施方案，制备靶向反应部分的反义组合物。 反应性部分优选包含通过具有或不具有插入插入部分的接头与靶向寡核苷酸缀合的一个或两个咪唑官能团。 还公开了治疗学，诊断和研究方法，合成核苷和可以被制成寡核苷酸的核苷片段也是公开的。

公开(公告)号：US08663932B2

公开(公告)日：2014-03-04

申请号：US13332738

申请日：2011-12-21

申请人： John Dresios ,  Richard Griffey

发明人： John Dresios ,  Richard Griffey

IPC分类号： G01N33/53 ,  C07K16/00

CPC分类号： G01N33/6887 ,  A61K9/1271 ,  A61K38/02 ,  A61K38/1777 ,  A61K39/44 ,  A61K47/42 ,  A61K47/6801 ,  A61K47/6803 ,  A61K47/6811 ,  A61K47/6847 ,  A61K49/0058 ,  C07K16/18 ,  G01N33/5088 ,  G01N33/567 ,  G01N33/6893 ,  G01N2333/47 ,  G01N2333/4712 ,  G01N2333/78

摘要： The present disclosure relates to methods for identifying proteins or peptide motifs of intracellular, extracellular, or extracellular matrix proteins specifically exposed in wound sites, as well as compositions for treating wounds, and methods for their use.

摘要翻译： 本公开涉及用于鉴定特异性暴露于伤口部位的细胞内，细胞外或细胞外基质蛋白以及用于治疗伤口的组合物的蛋白质或肽基序的方法及其使用方法。

公开(公告)号：US20080160512A1

公开(公告)日：2008-07-03

申请号：US11233630

申请日：2005-09-21

申请人： David J. Ecker ,  Richard Griffey ,  Rangarajan Sampath ,  Steven Hofstadler ,  John McNeil

发明人： David J. Ecker ,  Richard Griffey ,  Rangarajan Sampath ,  Steven Hofstadler ,  John McNeil

IPC分类号： C12Q1/68 ,  G06F19/00

CPC分类号： C12Q1/689 ,  C12Q1/686 ,  C12Q2600/156 ,  C12Q2565/627

摘要： Method for detecting and identifying unknown bioagents, including bacteria, viruses and the like, by a combination of nucleic acid amplification and molecular weight determination using primers which hybridize to conserved sequence regions of nucleic acids derived from a bioagent and which bracket variable sequence regions that uniquely identify the bioagent. The result is a “base composition signature” (BCS) which is then matched against a database of base composition signatures, by which the bioagent is identified.

摘要翻译： 通过核酸扩增和分子量测定的组合来检测和识别未知生物标签的方法，所述引物与衍生自生物制剂的核酸的保守序列区域杂交的引物以及独特的括号可变序列区域 识别生物制剂。 结果是“基本组成签名”（BCS），然后与基础组合签名的数据库进行匹配，通过该数据库识别生物标签。

公开(公告)号：US20070185046A1

公开(公告)日：2007-08-09

申请号：US11565781

申请日：2006-12-01

申请人： Balkrishen Bhat ,  Thazha Prakash ,  Prasad Dande ,  Charles Allerson ,  Richard Griffey ,  Eric Swayze

发明人： Balkrishen Bhat ,  Thazha Prakash ,  Prasad Dande ,  Charles Allerson ,  Richard Griffey ,  Eric Swayze

IPC分类号： A61K48/00 ,  C07H21/02

CPC分类号： C12N15/113 ,  C07H21/02 ,  C12N15/111 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/32 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/3231 ,  C12N2310/341 ,  C12N2310/346 ,  C12N2320/30 ,  C12N2320/51 ,  C12N2310/3521

摘要： The present invention provides double stranded compositions wherein each strand is modified to have a motif defined by positioning of β-D-ribonucleosides and sugar modified nucleosides. More particularly, the present compositions comprise one strand having an alternating motif and another strand having a hemimer motif, a blockmer motif, a fully modified motif or a positionally modified motif. At least one of the strands has complementarity to a nucleic acid target. The compositions are useful for targeting selected nucleic acid molecules and modulating the expression of one or more genes. In preferred embodiments the compositions of the present invention hybridize to a portion of a target RNA resulting in loss of normal function of the target RNA. The present invention also provides methods for modulating gene expression.

公开(公告)号：US20070179106A1

公开(公告)日：2007-08-02

申请号：US11565799

申请日：2006-12-01

申请人： Balkrishen Bhat ,  Thazha Prakash ,  Prasad Dande ,  Charles Allerson ,  Richard Griffey ,  Eric Swayze

发明人： Balkrishen Bhat ,  Thazha Prakash ,  Prasad Dande ,  Charles Allerson ,  Richard Griffey ,  Eric Swayze

IPC分类号： A61K48/00

CPC分类号： C12N15/113 ,  C07H21/02 ,  C12N15/111 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/32 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/3231 ,  C12N2310/341 ,  C12N2310/346 ,  C12N2320/30 ,  C12N2320/51 ,  C12N2310/3521

摘要： The present invention provides double stranded compositions wherein each strand is modified to have a motif defined by positioning of β-D-ribonucleosides and sugar modified nucleosides. More particularly, the present compositions comprise one strand having a gapped motif and another strand having a gapped motif, a hemimer motif, a blockmer motif, a fully modified motif, a positionally modified motif or an alternating motif. At least one of the strands has complementarity to a nucleic acid target. The compositions are useful for targeting selected nucleic acid molecules and modulating the expression of one or more genes. In some embodiments, the compositions of the present invention hybridize to a portion of a target RNA resulting in loss of normal function of the target RNA. The present invention also provides methods for modulating gene expression.

公开(公告)号：US20070173475A1

公开(公告)日：2007-07-26

申请号：US11565804

申请日：2006-12-01

申请人： Balkrishen Bhat ,  Thazha Prakash ,  Prasad Dande ,  Charles Allerson ,  Richard Griffey ,  Eric Swayze

发明人： Balkrishen Bhat ,  Thazha Prakash ,  Prasad Dande ,  Charles Allerson ,  Richard Griffey ,  Eric Swayze

IPC分类号： A61K48/00 ,  C07H21/02 ,  C07F9/6512

CPC分类号： C12N15/113 ,  C07H21/02 ,  C12N15/111 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/32 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/3231 ,  C12N2310/341 ,  C12N2310/346 ,  C12N2320/30 ,  C12N2320/51 ,  C12N2310/3521

摘要： The present invention provides double stranded compositions wherein each strand is modified to have a motif defined by positioning of β-D-ribonucleosides and sugar modified nucleosides. More particularly, the present compositions comprise one strand having a gapped motif and another strand having a gapped motif, a hemimer motif, a blockmer motif, a fully modified motif, a positionally modified motif or an alternating motif. At least one of the strands has complementarity to a nucleic acid target. The compositions are useful for targeting selected nucleic acid molecules and modulating the expression of one or more genes. In some embodiments, the compositions of the present invention hybridize to a portion of a target RNA resulting in loss of normal function of the target RNA. The present invention also provides methods for modulating gene expression.

公开(公告)号：US20070167391A1

公开(公告)日：2007-07-19

申请号：US11565841

申请日：2006-12-01

申请人： Balkrishen Bhat ,  Thazha Prakash ,  Prasad Dande ,  Charles Allerson ,  Richard Griffey ,  Eric Swayze

发明人： Balkrishen Bhat ,  Thazha Prakash ,  Prasad Dande ,  Charles Allerson ,  Richard Griffey ,  Eric Swayze

IPC分类号： A61K48/00 ,  C07H21/02

CPC分类号： C12N15/113 ,  C07H21/02 ,  C12N15/111 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/32 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/3231 ,  C12N2310/341 ,  C12N2310/346 ,  C12N2320/30 ,  C12N2320/51 ,  C12N2310/3521

摘要： The present invention provides double stranded compositions wherein each strand is modified to have a motif defined by positioning of β-D-ribonucleosides and sugar modified nucleosides. More particularly, the present compositions comprise one strand having a gapped motif and another strand having a gapped motif, a hemimer motif, a blockmer motif, a fully modified motif, a positionally modified motif or an alternating motif. At least one of the strands has complementarity to a nucleic acid target. The compositions are useful for targeting selected nucleic acid molecules and modulating the expression of one or more genes. In some embodiments, the compositions of the present invention hybridize to a portion of a target RNA resulting in loss of normal function of the target RNA. The present invention also provides methods for modulating gene expression.

公开(公告)号：US20060121520A1

公开(公告)日：2006-06-08

申请号：US11331987

申请日：2006-01-13

申请人： David Ecker ,  Richard Griffey ,  Rangarajan Sampath ,  Steven Hofstadler ,  John McNeil

发明人： David Ecker ,  Richard Griffey ,  Rangarajan Sampath ,  Steven Hofstadler ,  John McNeil

IPC分类号： C12Q1/68 ,  G06F19/00 ,  C07H21/04

CPC分类号： C12Q1/689 ,  C12Q1/686 ,  C12Q2600/156 ,  C12Q2565/627

摘要： Method for detecting and identifying unknown bioagents, including bacteria, viruses and the like, by a combination of nucleic acid amplification and molecular weight determination using primers which hybridize to conserved sequence regions of nucleic acids derived from a bioagent and which bracket variable sequence regions that uniquely identify the bioagent. The result is a “base composition signature” (BCS) which is then matched against a database of base composition signatures, by which the bioagent is identified.

公开(公告)号：US06969763B1

公开(公告)日：2005-11-29

申请号：US09310844

申请日：1999-05-12

申请人： David J. Ecker ,  Richard Griffey ,  Stanley T. Crooke ,  Ranga Sampath ,  Eric Swayze ,  Venkatraman Mohan ,  Steven Hofstadler ,  John McNeil

发明人： David J. Ecker ,  Richard Griffey ,  Stanley T. Crooke ,  Ranga Sampath ,  Eric Swayze ,  Venkatraman Mohan ,  Steven Hofstadler ,  John McNeil

IPC分类号： C07H21/04 ,  C07K14/54 ,  C07K14/55 ,  C40B30/02 ,  C40B50/02 ,  G06F19/00

CPC分类号： C40B50/02 ,  C07H21/04 ,  C07K14/5406 ,  C07K14/55 ,  C40B30/02 ,  G06F19/14 ,  G06F19/16 ,  G06F19/28

摘要： Methods for the identification of compounds which modulate, either inhibit or stimulate, biomolecules are provided. Nucleic acids, especially RNAs are preferred substrates for such modulation. The present methods are particularly powerful in that they provide novel combinations of techniques which give rise to compounds, usually “small” organic compounds, which are highly potent modulators of RNA and other biomolecular activity. In accordance with preferred aspects of the invention, very large numbers of compounds may be tested essentially simultaneously to determine whether they are likely to interact with a molecular interaction site and modulate the activity of the biomolecule. Pharmaceuticals, veterinary drugs, agricultural chemicals, industrial chemicals, research chemicals and many other beneficial compounds may be identified in accordance with embodiments of this invention.

摘要翻译： 提供了用于鉴定调节，抑制或刺激生物分子的化合物的方法。 核酸，特别是RNA是用于这种调节的优选底物。 本发明的方法特别强大，因为它们提供了引起化合物（通常是“小”）有机化合物的技术的新组合，其是RNA和其它生物分子活性的高效调节剂。 根据本发明的优选方面，可以基本上同时测试非常大量的化合物以确定它们是否可能与分子相互作用位点相互作用并且调节生物分子的活性。 根据本发明的实施方案，可以鉴定药物，兽药，农药，工业化学品，研究化学品和许多其它有益化合物。