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    STREPTOCOCCUS PNEUMONIAE DETECTION IN BLOOD
    2.
    发明申请
    STREPTOCOCCUS PNEUMONIAE DETECTION IN BLOOD 有权
    血清中肺炎支原体检测

    公开(公告)号：US20140363814A1

    公开(公告)日：2014-12-11

    申请号：US14356465

    申请日：2011-11-15

    申请人： Pierre-Alexandre Drèze ,  Pierre Smeesters ,  Lena Demazy ,  Laurence Van Melderen

    发明人： Pierre-Alexandre Drèze ,  Pierre Smeesters ,  Lena Demazy ,  Laurence Van Melderen

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/689 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q2600/112 ,  C12Q2600/16 ,  C12Q2527/125

    摘要： The present invention provides a method and a kit for detecting a S. pneumoniae nucleic acid in a sample. The method comprises the steps of isolating said nucleic acid and detecting hybridization between the S. pneumoniae nucleic acid and a labeled probe, wherein the detection of hybridization comprises detecting a change in signal from the labeled probe during or after hybridization relative to the labeled probe signal before hybridization. The method of the present invention is characterized in that said nucleic acid is a ribonucleic acid, which isolation comprises the addition of acidic phenol to said sample and the purification of said ribonucleic acid.

    摘要翻译： 本发明提供了用于检测样品中肺炎链球菌核酸的方法和试剂盒。 该方法包括分离所述核酸并检测肺炎链球菌核酸与标记探针之间的杂交的步骤，其中杂交检测包括在杂交期间或之后相对于标记的探针信号检测标记的探针的信号变化 杂交前。 本发明的方法的特征在于所述核酸是核糖核酸，其分离包括向所述样品中加入酸性苯酚和所述核糖核酸的纯化。

    Method for the selection of recombination clones comprising a sequence encoding an antidote protein to a toxic molecule
    3.
    发明授权
    Method for the selection of recombination clones comprising a sequence encoding an antidote protein to a toxic molecule 有权
    用于选择重组克隆的方法，其包含编码毒性分子的解毒剂蛋白质的序列

    公开(公告)号：US08470580B2

    公开(公告)日：2013-06-25

    申请号：US10468536

    申请日：2002-02-22

    申请人： Philippe Gabant ,  Laurence Van Melderen ,  Cédric Yves Szpirer

    发明人： Philippe Gabant ,  Laurence Van Melderen ,  Cédric Yves Szpirer

    IPC分类号： C12N1/21 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C12N15/70 ,  C07K14/245 ,  C12N15/10 ,  C12N15/1034 ,  C12N15/65 ,  C12N15/66

    摘要： The present invention is related to a method for the selection of recombinant clones having integrated a gene of interest and a nucleotide sequence encoding a functional antidote protein to a toxic molecule, wherein said recombinant clones are the ones which survive following their integration into a host cell comprising in its genome a nucleotide sequence encoding said toxic molecule. The present invention is also related to a nucleic acid construct, a vector comprising said nucleic acid construct, a host cell and a cloning and/or sequencing kit for performing said method.

    摘要翻译： 本发明涉及用于选择具有将感兴趣的基因与编码功能性解毒剂蛋白质的核苷酸序列集成到毒性分子的重组克隆的方法，其中所述重组克隆是在其整合入宿主细胞后能够存活的克隆 在其基因组中包含编码所述毒性分子的核苷酸序列。 本发明还涉及核酸构建体，包含所述核酸构建体的载体，宿主细胞和用于进行所述方法的克隆和/或测序试剂盒。