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公开(公告)号：US09938520B2
公开(公告)日：2018-04-10
申请号：US14650645
申请日：2013-12-11
申请人： QIAGEN GmbH
发明人： Roland Fabis
IPC分类号： B32B5/16 , C01B33/113 , C12N15/10 , C04B35/628 , C01G49/06 , C01G49/08 , C09C3/06 , C09C1/24 , C09C1/30
CPC分类号： C12N15/1013 , C01G49/06 , C01G49/08 , C01P2004/61 , C01P2004/62 , C01P2006/42 , C04B35/62807 , C04B35/62849 , C09C1/24 , C09C1/3054 , C09C3/063
摘要： The present invention provides a cost-efficient method for producing particles having a SiO2 containing surface wherein said method comprises a) providing an aqueous reaction composition comprising i) core particles, ii) an added base, iii) a silicate salt, and iv) a pH modulator wherein the pH value of the reaction composition is above the gelation pH value; b) agitating said reaction composition, wherein the pH modulator decreases the pH value of the reaction composition over time and wherein due to said decrease of the pH value of the reaction composition SiO2 is deposited onto the core particles, whereby particles are formed which have a diameter of 30 μm or less; and c) obtaining the particles. Furthermore, silica particles having high nucleic acid binding properties are provided.
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公开(公告)号：US20160281078A1
公开(公告)日：2016-09-29
申请号：US15160801
申请日：2016-05-20
申请人： Qiagen GmbH
发明人： Roland Fabis , Nadine Krüger , Jan Petzel
CPC分类号： C12N15/101 , B01D15/34 , B01D15/363 , B01D15/426 , C12N15/1013 , C12Q1/6806 , C12Q1/6874
摘要： The present invention pertains to a method for isolating nucleic acids by size from a sample comprising nucleic acids of different sizes using an anion exchange matrix, wherein nucleic acids of a preselected size or a preselected size range are isolated by varying the pH value during elution and/or binding.
摘要翻译： 本发明涉及使用阴离子交换基质从包含不同大小的核酸的样品中分离大小的核酸的方法，其中通过在洗脱期间改变pH值分离预选大小或预选大小范围的核酸，以及 /或绑定。
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公开(公告)号：US20220204968A1
公开(公告)日：2022-06-30
申请号：US17695270
申请日：2022-03-15
申请人： QIAGEN GmbH
发明人： Roland Fabis , Anke Homann , Thorsten Voss , Thomas Hanselle
摘要： The present invention relates to a lysis, binding and/or wash reagent for isolating and/or purifying nucleic acids and a method for isolating and/or purifying nucleic acids.
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公开(公告)号：US20170159042A1
公开(公告)日：2017-06-08
申请号：US15440699
申请日：2017-02-23
申请人： Qiagen GmbH
发明人： Roland Fabis , Anke Homann , Thorsten Voss , Thomas Hanselle
CPC分类号： C12N15/1006 , C12N1/06 , C12N15/1003
摘要： The present invention relates to a lysis, binding and/or wash reagent for isolating and/or purifying nucleic acids and a method for isolating and/or purifying nucleic acids.
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公开(公告)号：US20170159041A1
公开(公告)日：2017-06-08
申请号：US15440682
申请日：2017-02-23
申请人： Qiagen GmbH
发明人： Roland Fabis , Anke Homann , Thorsten Voss , Thomas Hanselle
IPC分类号： C12N15/10
CPC分类号： C12N15/1006 , C12N1/06 , C12N15/1003
摘要： The present invention relates to a lysis, binding and/or wash reagent for isolating and/or purifying nucleic acids and a method for isolating and/or purifying nucleic acids.
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公开(公告)号：US11253797B2
公开(公告)日：2022-02-22
申请号：US15204706
申请日：2016-07-07
申请人： Qiagen GmbH
发明人： Roland Fabis , Markus Müller , Jörg Hucklenbroich , Mario Scherer
IPC分类号： B01D15/34 , B01D15/22 , B01J20/291 , B01J20/285 , C07H1/08 , C07H21/04
摘要： The present invention relates to a chromatographic device for isolating and purifying nucleic acids, preferably genomic DNA, by gel filtration chromatography, a method for isolating and purifying nucleic acids, preferably genomic DNA, using this device and a kit comprising this device.
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公开(公告)号：US20220002708A1
公开(公告)日：2022-01-06
申请号：US17235403
申请日：2021-04-20
申请人： Qiagen GmbH
发明人： Roland Fabis , Anke Homann , Thorsten Voss , Thomas Hanselle
摘要： The present invention relates to a lysis, binding and/or wash reagent for isolating and/or purifying nucleic acids and a method for isolating and/or purifying nucleic acids.
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公开(公告)号：US20150307870A1
公开(公告)日：2015-10-29
申请号：US14650645
申请日：2013-12-11
申请人： QIAGEN GMBH
发明人： Roland Fabis
IPC分类号： C12N15/10
CPC分类号： C12N15/1013 , C01G49/06 , C01G49/08 , C01P2004/61 , C01P2004/62 , C01P2006/42 , C04B35/62807 , C04B35/62849 , C09C1/24 , C09C1/3054 , C09C3/063
摘要： The present invention provides a cost-efficient method for producing particles having a SiO2 containing surface wherein said method comprises a) providing an aqueous reaction composition comprising i) core particles, ii) an added base, iii) a silicate salt, and iv) a pH modulator wherein the pH value of the reaction composition is above the gelation pH value; b) agitating said reaction composition, wherein the pH modulator decreases the pH value of the reaction composition over time and wherein due to said decrease of the pH value of the reaction composition SiO2 is deposited onto the core particles, whereby particles are formed which have a diameter of 30 μm or less; and c) obtaining the particles. Furthermore, silica particles having high nucleic acid binding properties are provided.
摘要翻译： 本发明提供了一种用于生产具有含SiO2表面的颗粒的成本有效的方法，其中所述方法包括：a）提供水性反应组合物，其包含i）核心颗粒，ii）加入的碱，iii）硅酸盐，和iv） pH调节剂，其中反应组合物的pH值高于凝胶化pH值; b）搅拌所述反应组合物，其中所述pH调节剂随时间降低反应组合物的pH值，并且其中由于反应组合物SiO 2的pH值的所述降低沉积到芯颗粒上，由此形成颗粒，其具有 直径30μm以下; 和c）获得颗粒。 此外，提供了具有高核酸结合性质的二氧化硅颗粒。
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公开(公告)号：US20140243216A1
公开(公告)日：2014-08-28
申请号：US14347444
申请日：2012-09-25
申请人： Qiagen GmbH
发明人： Roland Fabis , Nadine Krüger , Jan Petzel
CPC分类号： C12N15/101 , B01D15/34 , B01D15/363 , B01D15/426 , C12N15/1013 , C12Q1/6806 , C12Q1/6874
摘要： The present invention pertains to a method for isolating nucleic acids by size from a sample comprising nucleic acids of different sizes using an anion exchange matrix, wherein nucleic acids of a preselected size or a preselected size range are isolated by varying the pH value during elution and/or binding.
摘要翻译： 本发明涉及使用阴离子交换基质从包含不同大小的核酸的样品中分离大小的核酸的方法，其中通过在洗脱期间改变pH值分离预选大小或预选大小范围的核酸，以及 /或绑定。
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