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公开(公告)号：US20170275612A1
公开(公告)日：2017-09-28
申请号：US15612091
申请日：2017-06-02
申请人： QVELLA CORPORATION
发明人： Samad TALEBPOUR , Aye Aye KHINE , Robert MAASKANT , Tino ALAVIE
摘要： Methods and devices are provided for pre-treatment of a sample containing microbial cells. In some embodiments, the pre-treatment of the sample is performed via the initial selective lysis, within a sample pre-treatment vessel, of non-microbial cells, such as blood cells, and the subsequent centrifugal separation of the sample to remove the resulting debris and concentrate the microbial cells. An immiscible and dense cushioning liquid may be included for collecting the microbial cells adjacent to the liquid interface formed by the cushioning liquid upon centrifugation of the pre-treatment vessel. After removal of a substantial quantity of the supernatant, resuspension of the collected microbial cells, and re-establishment of the cushioning liquid interface, at least a portion of the remaining suspension may be removed without substantially removing the cushioning liquid. One or more intermediate wash cycles may be performed prior to extraction of the remaining suspension, which provides a “pretreated” sample.
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公开(公告)号：US20140154687A1
公开(公告)日：2014-06-05
申请号：US13833872
申请日：2013-03-15
申请人： Qvella Corporation
发明人： Samad TALEBPOUR , Aye Aye KHINE , Robert MAASKANT , Tino ALAVIE
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6848 , B01L3/5021 , B01L3/50215 , B01L3/5027 , B01L7/52 , B01L2200/026 , B01L2300/046 , B01L2300/0816 , B01L2300/0864 , B01L2300/1833 , C12Q1/02 , C12Q1/6806 , C12Q2523/307 , C12Q2527/125 , C12Q2527/137
摘要： Methods and devices are provided for pretreatment of a sample containing microbial cells. In some embodiments, the pretreatment of the sample is performed via the initial selective lysis, within a sample pretreatment vessel, of non-microbial cells (such as blood cells) and the subsequent centrifugation of the sample to remove the resulting debris and concentrate the microbial cells. An immiscible and dense cushioning liquid may be included, for collecting the microbial cells adjacent to a liquid interface formed by the cushioning liquid, upon centrifugation of the pretreatment vessel. After removal of a substantial quantity of the supernatant, resuspension of the collected microbial cells, and re-establishment of the liquid interface, at least a portion of the remaining suspension may be removed without substantially removing the cushioning liquid. One or more intermediate wash cycles may be performed to prior to extraction of the pretreated sample.
摘要翻译： 提供了用于预处理含有微生物细胞的样品的方法和装置。 在一些实施方案中，样品的预处理通过在样品预处理容器内，非微生物细胞（例如血细胞）中的初始选择性裂解进行，随后将样品离心以除去所得碎片并浓缩微生物 细胞。 可以包括不混溶和致密的缓冲液体，用于在预处理容器离心时收集由缓冲液形成的液体界面附近的微生物细胞。 在除去大量的上清液后，重新悬浮收集的微生物细胞并重新建立液体界面，可以除去至少一部分剩余的悬浮液，而基本不除去缓冲液体。 可以在提取预处理样品之前进行一个或多个中间洗涤循环。
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3.发明申请公开(公告)号：US20210208128A1
公开(公告)日：2021-07-08
申请号：US17057088
申请日：2019-05-24
申请人： QVELLA CORPORATION
发明人： Samad TALEBPOUR , Aye Aye KHINE , Vilcy PARMAR , Sukhdev MANKU , Alaleh SAMIEI
摘要： Methods and compositions are provided for the selective lysis of eukaryotic cells and the separation of microbial cells. Blood cells and/or other eukaryotic cells in a sample, may be selectively lysed by adding, to the sample, a blood lysis reagent including saponin and an alkaline buffer, and optionally sodium polyanethole sulfonate and a non-ionic surfactant, thereby forming a mixture, and agitating the mixture. Microbial cells in the mixture may then be separated, for example, using a separation method such as centrifugation or filtration, and optionally detected or cultured in growth media. Blood lysis reagent compositions are provided that are suitable for preserving the intactness of microbial cells upon mixing with the sample. In example embodiments in which the sample is a blood sample, the blood lysis reagent composition may be selected to avoid or reduce the presence of visible blood debris upon centrifugation or filtration.
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公开(公告)号：US20140004501A1
公开(公告)日：2014-01-02
申请号：US13750723
申请日：2013-01-25
申请人： Qvella Corporation
发明人： Samad TALEBPOUR , Aye Aye KHINE , Robert MAASKANT , Tino ALAVIE
CPC分类号： C12Q1/6806 , B01L3/502715 , B01L3/502738 , B01L3/502753 , B01L7/52 , B01L2200/0631 , B01L2200/143 , B01L2300/0645 , B01L2300/0681 , B01L2300/0809 , B01L2300/0858 , B01L2300/087 , B01L2300/0874 , B01L2300/0887 , B01L2300/14 , B01L2300/1833 , B01L2400/0694 , C12M35/02 , C12M47/06 , C12N1/066 , C12N1/08 , C12N13/00 , C12Q1/686 , C12Q3/00
摘要： Devices and methods are provided for electrically lysing cells and releasing macromolecules from the cells. A microfluidic device is provided that includes a planar channel having a thickness on a submillimeter scale, and including electrodes on its upper and lower inner surfaces. After filling the channel with a liquid, such that the channel contains cells within the liquid, a series of voltage pulses of alternating polarity are applied between the channel electrodes, where the amplitude of the voltage pulses and a pulsewidth of the voltage pulses are effective for causing irreversible electroporation of the cells. The channel is configured to possess thermal properties such that the application of the voltage produces a rapid temperature rise as a result of Joule heating for releasing the macromolecules from the electroplated cells. The channel may also include an internal filter for capturing and concentrating the cells prior to electrical processing.
摘要翻译： 提供了用于电解裂解细胞并从细胞释放大分子的装置和方法。 提供一种微流体装置，其包括具有亚毫米级厚度的平面通道，并且在其上下表面上包括电极。 在用液体填充通道之后，使得通道在液体内包含单元，在通道电极之间施加一系列交替极性的电压脉冲，其中电压脉冲的幅度和电压脉冲的脉冲宽度对于 引起细胞的不可逆电穿孔。 该通道被配置为具有热性质，使得施加电压由于焦耳加热而导致快速升温，以从电镀细胞释放大分子。 通道还可以包括内部滤波器，用于在电处理之前捕获和集中单元。
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公开(公告)号：US20200291386A1
公开(公告)日：2020-09-17
申请号：US16881479
申请日：2020-05-22
申请人： QVELLA CORPORATION
发明人： Samad TALEBPOUR , Aye Aye KHINE , Robert MAASKANT , Tino ALAVIE
IPC分类号： C12N15/10 , B01L3/00 , C12Q1/6806 , C12Q1/24
摘要： Methods and devices are provided for pretreatment of a sample containing microbial cells. In some embodiments, the pretreatment of the sample is performed via the initial selective lysis, within a sample pretreatment vessel, of non-microbial cells (such as blood cells) and the subsequent centrifugal separation of the sample to remove the resulting debris and concentrate the microbial cells. An immiscible and dense cushioning liquid may be included for collecting the microbial cells adjacent to the liquid interface formed by the cushioning liquid upon centrifugation of the pretreatment vessel. After removal of a substantial quantity of the supernatant, resuspension of the collected microbial cells, and re-establishment of the cushioning liquid interface, at least a portion of the remaining suspension may be removed without substantially removing the cushioning liquid. One or more intermediate wash cycles may be performed prior to extraction of the remaining suspension, which provides a “pretreated” sample.
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公开(公告)号：US20160068897A1
公开(公告)日：2016-03-10
申请号：US14648161
申请日：2013-11-26
申请人： QVELLA CORPORATION
发明人： Samad TALEBPOUR , Aye Aye KHINE , Robert MAASKANT , Tino ALAVIE
IPC分类号： C12Q1/68
CPC分类号： B01L3/5021 , B01L3/50215 , B01L3/5027 , B01L7/52 , B01L2200/026 , B01L2300/046 , B01L2300/0816 , B01L2300/0864 , B01L2300/1833 , C12Q1/6806 , C12Q2523/307 , C12Q2527/125 , C12Q2527/137
摘要： Methods and devices are provided for pretreatment of a sample containing microbial cells. In some embodiments, the pretreatment of the sample is performed via the initial selective lysis, within a sample pretreatment vessel, of non-microbial cells (such as blood cells) and the subsequent centrifugal separation of the sample to remove the resulting debris and concentrate the microbial cells. An immiscible and dense cushioning liquid may be included for collecting the microbial cells adjacent to the liquid interface formed by the cushioning liquid upon centrifugation of the pretreatment vessel. After removal of a substantial quantity of the supernatant, resuspension of the collected microbial cells, and re-establishment of the cushioning liquid interface, at least a portion of the remaining suspension may be removed without substantially removing the cushioning liquid. One or more intermediate wash cycles may be performed prior to extraction of the remaining suspension, which provides a “pretreated” sample.
摘要翻译： 提供了用于预处理含有微生物细胞的样品的方法和装置。 在一些实施方案中，样品的预处理通过在样品预处理容器内，非微生物细胞（例如血细胞）中的初始选择性裂解进行，随后离心分离样品以除去所得碎片并将其浓缩 微生物细胞。 可以包括不混溶和致密的缓冲液体，用于在预处理容器离心时收集由缓冲液体形成的液体界面附近的微生物细胞。 在除去大量的上清液后，重新悬浮收集的微生物细胞并重新建立缓冲液体界面，可以除去至少一部分剩余的悬浮液，而基本不除去缓冲液体。 在提取剩余悬浮液之前可以进行一个或多个中间洗涤循环，其提供“预处理”样品。
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公开(公告)号：US20230416722A1
公开(公告)日：2023-12-28
申请号：US18208713
申请日：2023-06-12
申请人： QVELLA CORPORATION
发明人： Samad TALEBPOUR , Aye Aye KHINE , Robert MAASKANT , Tino ALAVIE
IPC分类号： C12N15/10 , B01L3/00 , C12Q1/6806 , C12Q1/24 , C08F220/28
CPC分类号： C12N15/1003 , B01L3/5021 , B01L3/5027 , C12Q1/6806 , C12Q1/24 , C08F220/286 , C08G2261/1452
摘要： Methods and devices are provided for pretreatment of a sample containing microbial cells. In some embodiments, the pretreatment of the sample is performed via the initial selective lysis, within a sample pretreatment vessel, of non-microbial cells (such as blood cells) and the subsequent centrifugal separation of the sample to remove the resulting debris and concentrate the microbial cells. An immiscible and dense cushioning liquid may be included for collecting the microbial cells adjacent to the liquid interface formed by the cushioning liquid upon centrifugation of the pretreatment vessel. After removal of a substantial quantity of the supernatant, resuspension of the collected microbial cells, and re-establishment of the cushioning liquid interface, at least a portion of the remaining suspension may be removed without substantially removing the cushioning liquid. One or more intermediate wash cycles may be performed prior to extraction of the remaining suspension, which provides a “pretreated” sample.
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公开(公告)号：US20170211128A1
公开(公告)日：2017-07-27
申请号：US15401291
申请日：2017-01-09
申请人： QVELLA CORPORATION
发明人： Samad TALEBPOUR , Aye Aye KHINE , Robert MAASKANT , Tino ALAVIE
CPC分类号： C12Q1/6806 , B01L3/502715 , B01L3/502738 , B01L3/502753 , B01L7/52 , B01L2200/0631 , B01L2200/143 , B01L2300/0645 , B01L2300/0681 , B01L2300/0809 , B01L2300/0858 , B01L2300/087 , B01L2300/0874 , B01L2300/0887 , B01L2300/14 , B01L2300/1833 , B01L2400/0694 , C12M35/02 , C12M47/06 , C12N1/066 , C12N1/08 , C12N13/00 , C12Q1/686 , C12Q3/00
摘要： Devices and methods are provided for electrically lysing cells and releasing macromolecules from the cells. A microfluidic device is provided that includes a planar channel having a thickness on a submillimeter scale, and including electrodes on its upper and lower inner surfaces. After filling the channel with a liquid, such that the channel contains cells within the liquid, a series of voltage pulses of alternating polarity are applied between the channel electrodes, where the amplitude of the voltage pulses and a pulsewidth of the voltage pulses are effective for causing irreversible electroporation of the cells. The channel is configured to possess thermal properties such that the application of the voltage produces a rapid temperature rise as a result of Joule heating for releasing the macromolecules from the electroplated cells. The channel may also include an internal filter for capturing and concentrating the cells prior to electrical processing.
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