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    Adipose tissue-derived stromal cell that expresses characteristics of a neuronal cell
    3.
    发明授权
    Adipose tissue-derived stromal cell that expresses characteristics of a neuronal cell 失效
    脂肪组织来源的表达神经细胞特征的基质细胞

    公开(公告)号：US07078230B2

    公开(公告)日：2006-07-18

    申请号：US09793173

    申请日：2001-02-26

    申请人： William O. Wilkison ,  Jeffrey Gimble

    发明人： William O. Wilkison ,  Jeffrey Gimble

    IPC分类号： C12N5/00

    CPC分类号： C12N5/0667 ,  A61K35/12 ,  A61K48/00 ,  C12N5/0618 ,  C12N5/067 ,  C12N2500/30 ,  C12N2500/44 ,  C12N2500/80 ,  C12N2506/1384

    摘要： The invention is in the area of pleuripotent stem cells generated from adipose tissue-derived stromal cells and uses thereof. In particular, the invention includes isolated adipose tissue derived stromal cells that have been induced to express at least one phenotypic characteristic of a neuronal, astroglial, hematopoietic progenitor, or hepatic cell. The invention also includes an isolated adipocyte tissue-derived stromal cell that has been dedifferentiated such that there is an absence of adipocyte phenotypic markers.

    摘要翻译： 本发明在由脂肪组织衍生的基质细胞产生的多能干细胞及其用途的领域中。 特别地，本发明包括被诱导表达神经元，星形胶质细胞，造血祖细胞或肝细胞的至少一种表型特征的分离的脂肪组织来源的基质细胞。 本发明还包括已经去分化的分离的脂肪细胞组织来源的基质细胞，使得没有脂肪细胞表型标志物。

    Method of using fish plasma components for tissue culture
    4.
    发明申请
    Method of using fish plasma components for tissue culture 审中-公开
    鱼血浆成分用于组织培养的方法

    公开(公告)号：US20050164389A1

    公开(公告)日：2005-07-28

    申请号：US11019083

    申请日：2004-12-21

    申请人： Evelyn Sawyer ,  Paul Janmey ,  Lisa Flanagan

    发明人： Evelyn Sawyer ,  Paul Janmey ,  Lisa Flanagan

    IPC分类号： C12N5/02 ,  C12N5/07 ,  C12N5/0793 ,  C12N5/00 ,  A01N1/00

    CPC分类号： C12N5/0601 ,  C12N5/0619 ,  C12N2500/80

    摘要： A tissue culture substrate for culturing mammalian stem cell tissue includes one or more specific fish plasma fractions, isolated from any plasma remainder, in a nutrient medium. The plasma fractions are extracted from plasma obtained from the blood of one or more fish that are progeny of domesticated broodstock reared under consistent and reproducible conditions.

    摘要翻译： 用于培养哺乳动物干细胞组织的组织培养底物包括在营养培养基中从任何血浆残留物中分离的一种或多种特定的鱼血浆级分。 从在一致和可重复的条件下饲养的驯养的亲体的后代的一种或多种鱼的血液中提取血浆级分。

    Activating egg extracts and method of preparation
    5.
    发明授权
    Activating egg extracts and method of preparation 失效
    活化蛋提取物及其制备方法

    公开(公告)号：US06245567B1

    公开(公告)日：2001-06-12

    申请号：US09050380

    申请日：1998-03-30

    申请人： Lawrence J. Wangh

    发明人： Lawrence J. Wangh

    IPC分类号： C12N506

    CPC分类号： C12N15/873 ,  A01K2217/05 ,  C12N5/061 ,  C12N2500/80 ,  C12N2501/01 ,  C12N2501/70 ,  C12N2517/10 ,  C12Q1/6841 ,  C12Q1/70

    摘要： The present invention concerns products and methods particularly useful for activating and analyzing non-dividing cell nuclei. The featured products include activating egg extracts, cytostatic factor (CSF) extracts, kits containing these extracts, and a microchamber microscope slide useful in analyzing nucleus activation.

    摘要翻译： 本发明涉及特别可用于激活和分析非分裂细胞核的产物和方法。 特色产品包括活化卵提取物，细胞生长因子（CSF）提取物，含有这些提取物的试剂盒和用于分析细胞核激活的微量显微镜载玻片。

    Mammalian multipotent neural stem cells
    6.
    发明授权
    Mammalian multipotent neural stem cells 失效
    哺乳动物多能神经干细胞

    公开(公告)号：US5849553A

    公开(公告)日：1998-12-15

    申请号：US485612

    申请日：1995-06-07

    申请人： David J. Anderson ,  Derek L. Stemple

    发明人： David J. Anderson ,  Derek L. Stemple

    IPC分类号： C07K16/28 ,  C12N5/0797 ,  C12N15/85 ,  C12N15/09

    CPC分类号： C07K16/2863 ,  C12N5/0623 ,  C12N2500/20 ,  C12N2500/25 ,  C12N2500/35 ,  C12N2500/38 ,  C12N2500/46 ,  C12N2500/80 ,  C12N2500/90 ,  C12N2501/02 ,  C12N2501/11 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2501/392 ,  C12N2501/395 ,  C12N2501/86 ,  C12N2510/00 ,  C12N2533/32 ,  C12N2533/52 ,  C12N2533/56

    摘要： The invention includes mammalian multipotent neural stem cells and their progeny and methods for the isolation and clonal propagation of such cells. At the clonal level the stem cells are capable of self regeneration and asymmetrical division. Lineage restriction is demonstrated within developing clones which are sensitive to the local environment. The invention also includes such cells which are transfected with foreign nucleic acid, e.g., to produce an immortalized neural stem cell, and immortalized cell lines which are capable of subsequent disimmortalization. The invention further includes transplantation assays which allow for the identification of mammalian multipotent neural stem cells from various tissues and methods for transplanting mammalian neural stem cells and/or neural or glial progenitors into mammals. A novel method for detecting antibodies to neural cell surface markers is disclosed as well as a monoclonal antibody to mouse LNGFR.

    摘要翻译： 本发明包括哺乳动物多能神经干细胞及其后代和用于分离和克隆繁殖这些细胞的方法。 在克隆级别，干细胞能够自我再生和不对称分裂。 在对本地环境敏感的开发克隆中证明了谱系限制。 本发明还包括用外源核酸转染的细胞，例如产生永生化神经干细胞，以及能够随后进行破碎的永生化细胞系。 本发明还包括允许从各种组织鉴定哺乳动物多能神经干细胞的移植测定法和用于将哺乳动物神经干细胞和/或神经或神经胶质祖细胞移植到哺乳动物中的方法。 公开了一种用于检测神经细胞表面标志物的抗体的新方法以及小鼠LNGFR的单克隆抗体。

    In vitro method for obtaining an isolated population of mammalian neural crest stem cells
    7.
    发明授权
    In vitro method for obtaining an isolated population of mammalian neural crest stem cells 失效
    用于获得哺乳动物神经嵴干细胞群体的体外方法

    公开(公告)号：US5824489A

    公开(公告)日：1998-10-20

    申请号：US290229

    申请日：1994-08-15

    申请人： David J. Anderson ,  Derek L. Stemple

    发明人： David J. Anderson ,  Derek L. Stemple

    IPC分类号： A01K67/027 ,  C12N5/0797 ,  C12N5/10 ,  C12N15/09 ,  C12P21/08 ,  C12R1/91 ,  G01N33/577 ,  C12N5/00

    CPC分类号： C12N5/0623 ,  C12N2500/20 ,  C12N2500/25 ,  C12N2500/35 ,  C12N2500/38 ,  C12N2500/46 ,  C12N2500/80 ,  C12N2500/90 ,  C12N2501/02 ,  C12N2501/11 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2501/392 ,  C12N2501/395 ,  C12N2501/86 ,  C12N2510/00 ,  C12N2533/32 ,  C12N2533/52 ,  C12N2533/56

    摘要： The invention includes methods for the isolation and clonal propagation of mammalian neural stem cells. The methods employ a novel separation and culturing regimen and bioassays for establishing the generation of neural stem cell derivatives. These methods result in the production of non-transformed neural stem cells and their progeny. The invention demonstrates, at the clonal level, the self regeneration and asymmetrical division of mammalian neural stem cells for the first time in feeder cell-independent cultures. Lineage restriction is demonstrated within a developing clone and is shown to be sensitive to the local environment. Multipotent neural stem cells cultured on a mixed substrate of poly-D-lysine and fibronectin generate PNS neurons and glia, but on fibronectin alone the stem cells generate PNS glia but not neurons. The neurogenic potential of the stem cells, while not expressed, is maintained over time on fibronectin. The invention further includes transplantation assays which allow for the identification of mammalian neural stem cells from various tissues. It also includes methods for transplanting mammalian neural stem cells and/or neural or glial progenitors into mammals.

    摘要翻译： 本发明包括哺乳动物神经干细胞分离和克隆繁殖的方法。 该方法采用新型分离培养方案和生物测定法，用于建立神经干细胞衍生物的产生。 这些方法导致未转化的神经干细胞及其后代的产生。 本发明在克隆水平上证明哺乳动物神经干细胞在饲养细胞非依赖性培养物中的自我再生和不对称分裂。 谱系限制在开发的克隆中证明，并且显示出对局部环境敏感。 在多聚赖氨酸和纤连蛋白的混合底物上培养的多能神经干细胞产生PNS神经元和神经胶质细胞，但是单独的纤连蛋白上，干细胞产生PNS神经胶质，而不是神经元。 干细胞的神经源性潜力虽然未被表达，但随着时间的推移在纤连蛋白上保持。 本发明还包括允许从各种组织鉴定哺乳动物神经干细胞的移植测定。 它还包括将哺乳动物神经干细胞和/或神经或神经胶质祖细胞移植到哺乳动物中的方法。

    Genetically engineered mammalian neural crest stem cells
    8.
    发明授权
    Genetically engineered mammalian neural crest stem cells 失效
    基因工程哺乳动物神经嵴干细胞

    公开(公告)号：US5654183A

    公开(公告)日：1997-08-05

    申请号：US188286

    申请日：1994-01-28

    申请人： David J. Anderson ,  Derek L. Stemple

    发明人： David J. Anderson ,  Derek L. Stemple

    IPC分类号： C12N5/0797 ,  C12N15/85 ,  C12N15/00

    CPC分类号： C12N5/0623 ,  C12N2500/20 ,  C12N2500/25 ,  C12N2500/35 ,  C12N2500/38 ,  C12N2500/46 ,  C12N2500/80 ,  C12N2500/90 ,  C12N2501/02 ,  C12N2501/11 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2501/392 ,  C12N2501/395 ,  C12N2501/86 ,  C12N2510/00 ,  C12N2533/32 ,  C12N2533/52 ,  C12N2533/56

    摘要： The invention includes mammalian multipotent neural stem cells and their progeny and methods for the isolation and clonal propagation of such cells. At the clonal level the stem cells are capable of self regeneration and asymmetrical division. Lineage restriction is demonstrated within developing clones which are sensitive to the local environment. The invention also includes such cells which are transfected with foreign nucleic acid, e.g., to produce an immortalized neural stem cell. The invention further includes transplantation assays which allow for the identification of mammalian multipotent neural stem cells from various tissues and methods for transplanting mammalian neural stem cells and/or neural or glial progenitors into mammals. A novel method for detecting antibodies to neural cell surface markers is disclosed as well as a monoclonal antibody to mouse LNGFR.

    摘要翻译： 本发明包括哺乳动物多能神经干细胞及其后代和用于分离和克隆繁殖这些细胞的方法。 在克隆级别，干细胞能够自我再生和不对称分裂。 在对本地环境敏感的开发克隆中证明了谱系限制。 本发明还包括用外源核酸转染的细胞，例如产生永生化的神经干细胞。 本发明还包括允许从各种组织鉴定哺乳动物多能神经干细胞的移植测定法和用于将哺乳动物神经干细胞和/或神经或神经胶质祖细胞移植到哺乳动物中的方法。 公开了一种用于检测神经细胞表面标志物的抗体的新方法以及小鼠LNGFR的单克隆抗体。

    Method for culturing insect cells in a medium containing fish serum
    9.
    发明授权
    Method for culturing insect cells in a medium containing fish serum 失效
    在含有鱼血清的培养基中培养昆虫细胞的方法

    公开(公告)号：US5498540A

    公开(公告)日：1996-03-12

    申请号：US362236

    申请日：1994-12-22

    申请人： Evelyn S. Sawyer ,  Philip J. Sawyer

    发明人： Evelyn S. Sawyer ,  Philip J. Sawyer

    IPC分类号： C12N5/07 ,  C12N5/00 ,  A61K35/56 ,  A61K35/60

    CPC分类号： C12N5/0601 ,  C12N2500/80

    摘要： A method for culturing insect cells using fish serum. The method uses serum extracted from the blood of fish to culture insect cells for various purposes. The technique has the key advantages of consistent quality, low cross reactivity, safety from infectious agents that would endanger researchers or humans and animals receiving cell culture products as therapy and has appropriate nutrients to maintain the growth of insect cells. Fish serum is used together with designated defined medium to allow insect cells to grow and populations to be maintained. The method may also be used with lumpfish to prevent the attachment of insect cells to cultureware. Serum is derived from the blood of captive stocks of fishes raised under control conditions.

    摘要翻译： 使用鱼血清培养昆虫细胞的方法。 该方法使用从鱼血液提取的血清培养昆虫细胞用于各种目的。 该技术具有质量一致，交叉反应性低，传染性物质的安全性，这将危及研究人员或接受细胞培养产物作为治疗的人类和动物，并具有维持昆虫细胞生长的适当营养。 鱼血清与指定的定义培养基一起使用，以使昆虫细胞生长并维持群体。 该方法还可以与小鱼一起使用以防止昆虫细胞附着到培养物上。 血清来源于在控制条件下养殖的鱼类养殖的血液。