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11 条记录 (耗时 0.045 秒)

    Fumonisin detoxification compositions and methods
    3.
    发明授权
    Fumonisin detoxification compositions and methods 有权
    富马尼素解毒组合物和方法

    公开(公告)号：US06514749B1

    公开(公告)日：2003-02-04

    申请号：US09885876

    申请日：2001-06-20

    申请人： Jonathan Duvick ,  Joyce R. Maddox ,  Tracy A. Rood

    发明人： Jonathan Duvick ,  Joyce R. Maddox ,  Tracy A. Rood

    IPC分类号： C12N114

    CPC分类号： A01N63/00 ,  C12N9/00 ,  C12N9/16 ,  C12N9/18 ,  C12N15/8209 ,  C12N15/8242 ,  C12N15/8282

    摘要： Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin and/or capable of growing on fumonisin as a sole carbon source. Using this method, several organisms have been identified. These organisms can be used to isolate the enzymes and the genes responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for fumonisin degrading enzymes can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.

    摘要翻译： 鉴定能够降解伏马菌素的生物体的方法。 可以将烟草毒素掺入到培养基中，用于选择对伏马菌素抗性的生物体和/或能够生长在作为唯一碳源的伏马毒素上。 使用这种方法，已经确定了几种生物体。 这些生物可用于分离酶和负责赋予伏马菌素抗性的基因。 可以将该基因克隆并插入到合适的表达载体中，以使蛋白质进一步表征。 此外，编码伏马菌素降解酶的DNA可用于转化通常易受镰刀菌或其他产生毒素的真菌感染敏感的植物细胞。 植物可以从转化的植物细胞中再生。 以这种方式，可以生产具有降解伏马菌素的能力以及产生降解酶的能力的转基因植物。 还公开了谷物，谷物加工，青贮饲料，粮食作物，动物饲料和瘤胃微生物的解毒方法。

    Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use
    6.
    发明申请
    Amino polyol amine oxidase polynucleotides and related polypeptides and methods of use 有权
    氨基多胺胺氧化酶多核苷酸及相关多肽及其使用方法

    公开(公告)号：US20060162009A1

    公开(公告)日：2006-07-20

    申请号：US10743891

    申请日：2003-12-22

    申请人： Jonathan Duvick ,  Jacob Gilliam ,  Joyce Maddox ,  Aragula Rao ,  Oswald Crasta ,  Otto Folkerts

    发明人： Jonathan Duvick ,  Jacob Gilliam ,  Joyce Maddox ,  Aragula Rao ,  Oswald Crasta ,  Otto Folkerts

    IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N9/06 ,  C12N15/82 ,  C12N5/04

    CPC分类号： C12N9/0022 ,  C12N15/8242 ,  C12N15/8282

    摘要： The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera and Rhinocladiella atrovirens. The polynucleotides may be mutated to remove glycosylation sites and cysteine residues. Additionally, the present invention provides recombinant expression cassettes, host cells, transgenic plants, and transgenic seed. The present invention also provides for polynucleotides containing both APAO and a fumonisin esterase. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and eukaryotic systems, methods for detecting fumonisins, and methods for identifying transformed plant cells. Methods for degrading fungal toxins in plants, grain, grain processing, silage, food crops and in animal feed are also disclosed.

    摘要翻译： 本发明提供了从Exophiala spinifera和Rhinocladiella atvirens分离的酶APAO的多核苷酸和相关多肽。 可以突变多核苷酸以除去糖基化位点和半胱氨酸残基。 另外，本发明提供了重组表达盒，宿主细胞，转基因植物和转基因种子。 本发明还提供了含有APAO和伏马菌素酯酶的多核苷酸。 此外，本发明提供了在原核和真核系统中生产APAO酶的方法，用于检测伏马毒素的方法和用于鉴定转化的植物细胞的方法。 还公开了降解植物，谷物，谷物加工，青贮饲料，粮食作物和动物饲料中真菌毒素的方法。

    Method for identifying activators of gene transcription
    7.
    发明申请
    Method for identifying activators of gene transcription 审中-公开
    识别基因转录激活子的方法

    公开(公告)号：US20050266447A1

    公开(公告)日：2005-12-01

    申请号：US11100722

    申请日：2005-04-07

    申请人： Jonathan Duvick ,  Maria Fedorova

    发明人： Jonathan Duvick ,  Maria Fedorova

    IPC分类号： C12N15/10 ,  C12N15/11 ,  C12N15/63 ,  C12Q1/68

    CPC分类号： C12Q1/6897 ,  C12N15/1086

    摘要： Methods and compositions for identifying a nucleotide sequence that encodes a polypeptide that directly or indirectly modulates the activity of a transcriptional regulatory region are provided. The methods permit the efficient screening of a large number of candidate nucleotide sequences for potential modulators of transcription. In particular, a population of polynucleotide constructs comprising a common transcriptional regulatory region operably linked to a candidate sequence is introduced into a population of host cells, and each candidate sequence is screened for modulation in its expression level, for example, using methods that employ hybridization to a complementary DNA microarray. The expression level of the candidate sequence correlates to the activity of the transcriptional regulatory region. Thus, alterations in the expression level of a candidate sequence indicate that the sequence encodes a polypeptide that modulates the activity of the transcriptional regulatory region. Compositions for practicing the invention are further provided.

    摘要翻译： 提供了用于鉴定编码直接或间接调节转录调节区活性的多肽的核苷酸序列的方法和组合物。 该方法允许有效筛选潜在的转录调控因子的大量候选核苷酸序列。 特别地，将包含可操作地连接到候选序列的常见转录调节区的多核苷酸构建体的群体引入宿主细胞群体中，并且筛选每个候选序列以其表达水平进行调节，例如使用采用杂交的方法 到互补的DNA微阵列。 候选序列的表达水平与转录调控区的活性相关。 因此，候选序列的表达水平的改变表明序列编码调节转录调节区活性的多肽。 进一步提供实施本发明的组合物。

    Fumonisin detoxification compositions and methods
    8.
    发明授权
    Fumonisin detoxification compositions and methods 失效

    公开(公告)号：US06573075B1

    公开(公告)日：2003-06-03

    申请号：US09885901

    申请日：2001-06-20

    申请人： Jonathan Duvick ,  Xun Wang

    发明人： Jonathan Duvick ,  Xun Wang

    IPC分类号： C12N916

    CPC分类号： A01N63/00 ,  C12N9/00 ,  C12N9/16 ,  C12N9/18 ,  C12N15/8209 ,  C12N15/8242 ,  C12N15/8282

    摘要： Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin and/or capable of growing on fumonisin as a sole carbon source. Using this method, several organisms have been identified. These organisms can be used to isolate the enzymes and the genes responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for fumonisin degrading enzymes can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.

    Fumonisin detoxification compositions and methods
    9.
    发明授权
    Fumonisin detoxification compositions and methods 有权

    公开(公告)号：US06239330B1

    公开(公告)日：2001-05-29

    申请号：US09262758

    申请日：1999-03-04

    申请人： Jonathan Duvick ,  Joyce R. Maddox ,  Xun Wang

    发明人： Jonathan Duvick ,  Joyce R. Maddox ,  Xun Wang

    IPC分类号： C12N1531

    CPC分类号： A01N63/00 ,  C12N9/00 ,  C12N9/16 ,  C12N9/18 ,  C12N15/8209 ,  C12N15/8242 ,  C12N15/8282

    摘要： Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin and/or capable of growing on fumonisin as a sole carbon source. Using this method, several organisms have been identified. These organisms can be used to isolate the enzymes and the genes responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for fumonisin degrading enzymes can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.

    Fumonisin detoxification compositions and methods
    10.
    发明授权
    Fumonisin detoxification compositions and methods 失效

    公开(公告)号：US06229071B1

    公开(公告)日：2001-05-08

    申请号：US08888950

    申请日：1997-07-07

    申请人： Jonathan Duvick ,  Joyce R. Maddox ,  Tracy A. Rood ,  Xun Wang

    发明人： Jonathan Duvick ,  Joyce R. Maddox ,  Tracy A. Rood ,  Xun Wang

    IPC分类号： A01H106

    CPC分类号： A01N63/00 ,  C12N9/00 ,  C12N9/16 ,  C12N9/18 ,  C12N15/8209 ,  C12N15/8242 ,  C12N15/8282

    摘要： Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin and/or capable of growing on fumonisin as a sole carbon source. Using this method, several organisms have been identified. These organisms can be used to isolate the enzymes and the genes responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for fumonisin degrading enzymes can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.