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    Environmental containment systems
    3.
    发明授权
    Environmental containment systems 有权
    环境遏制系统

    公开(公告)号：US07819934B2

    公开(公告)日：2010-10-26

    申请号：US11879033

    申请日：2007-07-13

    申请人： Parrish M. Galliher ,  Geoffrey L. Hodge ,  Michael Fisher

    发明人： Parrish M. Galliher ,  Geoffrey L. Hodge ,  Michael Fisher

    IPC分类号： B01D50/00

    CPC分类号： C12M41/14 ,  B08B15/02 ,  C12M23/52 ,  C12M37/02 ,  C12M41/00 ,  C12M41/40 ,  Y10S55/18

    摘要： Environmental containment systems, and in certain embodiments, systems and methods involving vessels and unit operations or components of cell culture, cell containment, bioreactor, chemical manufacturing, or pharmaceutical manufacturing systems provided with environmental containment are provided. Certain vessels, unit operations, devices, and components may be used to perform all or part of biological, chemical, and/or pharmaceutical manufacturing processes therein. In some embodiments, an environmental containment system includes a multi-level containment apparatus. For instance, a first substantially closed environmental containment enclosure may be contained within a second substantially closed environmental containment enclosure; the first and second enclosures may be contained in a third substantially closed environmental containment enclosure, etc. Each of the environments within the substantially closed environmental containment enclosures may be controlled independently, and leakage of any materials from an inner system may be contained by an outer system.

    摘要翻译： 提供环境容纳系统，并且在某些实施方案中，提供了涉及容器和单元操作的系统和方法或细胞培养物，细胞容纳物，生物反应器，化学制造或具有环境容纳物的药物制造系统。 某些容器，单元操作，装置和部件可用于执行其中的生物，化学和/或药物制造过程的全部或部分。 在一些实施例中，环境容纳系统包括多级容纳装置。 例如，第一基本封闭的环境容纳外壳可以包含在第二基本封闭的环境容纳外壳内; 第一和第二外壳可以包含在第三基本封闭的环境容纳外壳等中。基本封闭的环境容纳外壳内的每个环境可以独立地被控制，并且任何材料从内部系统的泄漏可以被外部 系统。

    Continuous perfusion bioreactor system
    6.
    发明授权
    Continuous perfusion bioreactor system 有权
    连续灌注生物反应器系统

    公开(公告)号：US09109193B2

    公开(公告)日：2015-08-18

    申请号：US12124039

    申请日：2008-05-20

    申请人： Parrish M. Galliher ,  Geoffrey L. Hodge ,  Michael Fisher ,  Patrick Guertin ,  Dan Mardirosian

    发明人： Parrish M. Galliher ,  Geoffrey L. Hodge ,  Michael Fisher ,  Patrick Guertin ,  Dan Mardirosian

    IPC分类号： C12N5/02 ,  C12M1/00 ,  C12M3/00 ,  C12M1/06

    CPC分类号： C12M29/10 ,  B01D29/13 ,  C12M23/14 ,  C12M23/26 ,  C12M23/48 ,  C12M27/02 ,  C12M27/20 ,  C12M29/00 ,  C12M29/18 ,  C12M47/10

    摘要： Systems and methods for containing and manipulating liquids, including vessels and unit operations or components of cell culture, cell containment, bioreactor, and/or pharmaceutical manufacturing systems, are provided. In certain embodiments, such vessels and unit operations are directed to continuous perfusion reactor or bioreactor systems and may include one or more disposable components. For instance, in one aspect, a system includes an apparatus in the form of a bioreactor for harvesting cells which produce one or more products. The apparatus may include a disposable, collapsible bag adapted for containing a liquid, the collapsible bag in fluid communication with a liquid-solids (e.g., cell) separation device. For example, an outlet of the collapsible bag may be connected to an inlet of the separation device, and an outlet of the separation device may be connected to an inlet of the collapsible bag for recycle. Accordingly, after separating the cells from the liquid in the separation device, the cells can be flowed back into the collapsible bag where they can be re-harvested. Meanwhile, product contained in the liquid can be collected in a separate container. The efficiency of product formation in such a system may be enhanced by using mixing systems described herein.

    摘要翻译： 提供了用于容纳和操纵液体的系统和方法，包括容器和单元操作或细胞培养物，细胞容纳物，生物反应器和/或药物制造系统的组分。 在某些实施方案中，这样的容器和单元操作涉及连续灌注反应器或生物反应器系统，并且可以包括一个或多个一次性组件。 例如，一方面，系统包括生物反应器形式的装置，用于收集产生一种或多种产品的细胞。 该装置可以包括适于容纳液体的一次性可折叠袋，该可折叠袋与液体 - 固体（例如，池）分离装置流体连通。 例如，可折叠袋的出口可以连接到分离装置的入口，并且分离装置的出口可以连接到可折叠袋的入口以用于再循环。 因此，在分离装置中的液体分离细胞后，细胞可以流回到可收缩的袋子中，在那里它们可以被再次收获。 同时，包含在液体中的产品可以收集在单独的容器中。 通过使用本文所述的混合系统可以提高这种系统中产物形成的效率。

    CONTINUOUS PERFUSION BIOREACTOR SYSTEM
    7.
    发明申请
    CONTINUOUS PERFUSION BIOREACTOR SYSTEM 有权
    连续曝光生物反应器系统

    公开(公告)号：US20090035856A1

    公开(公告)日：2009-02-05

    申请号：US12124039

    申请日：2008-05-20

    申请人： Parrish M. Galliher ,  Geoffrey L. Hodge ,  Michael Fisher ,  Patrick Guertin ,  Dan Mardirosian

    发明人： Parrish M. Galliher ,  Geoffrey L. Hodge ,  Michael Fisher ,  Patrick Guertin ,  Dan Mardirosian

    IPC分类号： C12N5/06 ,  C12M1/00

    CPC分类号： C12M29/10 ,  B01D29/13 ,  C12M23/14 ,  C12M23/26 ,  C12M23/48 ,  C12M27/02 ,  C12M27/20 ,  C12M29/00 ,  C12M29/18 ,  C12M47/10

    摘要： Systems and methods for containing and manipulating liquids, including vessels and unit operations or components of cell culture, cell containment, bioreactor, and/or pharmaceutical manufacturing systems, are provided. In certain embodiments, such vessels and unit operations are directed to continuous perfusion reactor or bioreactor systems and may include one or more disposable components. For instance, in one aspect, a system includes an apparatus in the form of a bioreactor for harvesting cells which produce one or more products. The apparatus may include a disposable, collapsible bag adapted for containing a liquid, the collapsible bag in fluid communication with a liquid-solids (e.g., cell) separation device. For example, an outlet of the collapsible bag may be connected to an inlet of the separation device, and an outlet of the separation device may be connected to an inlet of the collapsible bag for recycle. Accordingly, after separating the cells from the liquid in the separation device, the cells can be flowed back into the collapsible bag where they can be re-harvested. Meanwhile, product contained in the liquid can be collected in a separate container. The efficiency of product formation in such a system may be enhanced by using mixing systems described herein.

    摘要翻译： 提供了用于容纳和操纵液体的系统和方法，包括容器和单元操作或细胞培养物，细胞容纳物，生物反应器和/或药物制造系统的组分。 在某些实施方案中，这样的容器和单元操作涉及连续灌注反应器或生物反应器系统，并且可以包括一个或多个一次性组件。 例如，一方面，系统包括生物反应器形式的装置，用于收集产生一种或多种产品的细胞。 该装置可以包括适于容纳液体的一次性可折叠袋，该可折叠袋与液体 - 固体（例如，池）分离装置流体连通。 例如，可折叠袋的出口可以连接到分离装置的入口，并且分离装置的出口可以连接到可折叠袋的入口以用于再循环。 因此，在分离装置中的液体分离细胞后，细胞可以流回到可收缩的袋子中，在那里它们可以被再次收获。 同时，包含在液体中的产品可以收集在单独的容器中。 通过使用本文所述的混合系统可以提高这种系统中产物形成的效率。

    Environmental containment systems
    9.
    发明申请
    Environmental containment systems 有权
    环境遏制系统

    公开(公告)号：US20080139865A1

    公开(公告)日：2008-06-12

    申请号：US11879033

    申请日：2007-07-13

    申请人： Parrish M. Galliher ,  Geoffrey L. Hodge ,  Michael Fisher

    发明人： Parrish M. Galliher ,  Geoffrey L. Hodge ,  Michael Fisher

    IPC分类号： B09B5/00

    CPC分类号： C12M41/14 ,  B08B15/02 ,  C12M23/52 ,  C12M37/02 ,  C12M41/00 ,  C12M41/40 ,  Y10S55/18

    摘要： Environmental containment systems, and in certain embodiments, systems and methods involving vessels and unit operations or components of cell culture, cell containment, bioreactor, chemical manufacturing, or pharmaceutical manufacturing systems provided with environmental containment are provided. Certain vessels, unit operations, devices, and components may be used to perform all or part of biological, chemical, and/or pharmaceutical manufacturing processes therein. In some embodiments, an environmental containment system includes a multi-level containment apparatus. For instance, a first substantially closed environmental containment enclosure may be contained within a second substantially closed environmental containment enclosure; the first and second enclosures may be contained in a third substantially closed environmental containment enclosure, etc. Each of the environments within the substantially closed environmental containment enclosures may be controlled independently, and leakage of any materials from an inner system may be contained by an outer system.

    摘要翻译： 提供环境容纳系统，并且在某些实施方案中，提供了涉及容器和单元操作的系统和方法或细胞培养物，细胞容纳物，生物反应器，化学制造或具有环境容纳物的药物制造系统。 某些容器，单元操作，装置和部件可用于执行其中的生物，化学和/或药物制造过程的全部或部分。 在一些实施例中，环境容纳系统包括多级容纳装置。 例如，第一基本封闭的环境容纳外壳可以包含在第二基本封闭的环境容纳外壳内; 第一和第二外壳可以包含在第三基本封闭的环境容纳外壳等中。基本封闭的环境容纳外壳内的每个环境可以独立地被控制，并且任何材料从内部系统的泄漏可以被外部 系统。

    Multi-variant cell indication technique
    10.
    发明申请
    Multi-variant cell indication technique 审中-公开
    多变细胞指示技术

    公开(公告)号：US20090191542A1

    公开(公告)日：2009-07-30

    申请号：US11895057

    申请日：2007-08-23

    申请人： Geoffrey L. Hodge ,  Shari Brezinsky

    发明人： Geoffrey L. Hodge ,  Shari Brezinsky

    IPC分类号： C12Q1/68 ,  C12Q1/02 ,  C12Q1/26 ,  G01N33/53 ,  C12N5/06 ,  G06Q90/00

    CPC分类号： G01N33/5023 ,  G01N2333/906 ,  G06Q99/00

    摘要： The invention provides methods for identifying and isolating cells that express high levels of protein. A method is described for identifying a cell or cell line expressing a desired protein comprising determining in a cell or cell line that has been transfected with a vector comprising a nucleotide sequence encoding the desired protein the intracellular expression levels of elements of the vector and also determining expression of the desired protein on the surface of the cell or cell line, in certain cases in a single assay.

    摘要翻译： 本发明提供用于鉴定和分离表达高水平蛋白质的细胞的方法。 描述了用于鉴定表达所需蛋白质的细胞或细胞系的方法，包括在已经用包含编码所需蛋白质的核苷酸序列的载体转染的细胞或细胞系中测定载体元件的细胞内表达水平，并确定 在单个测定中，在某些情况下，在细胞或细胞系的表面上表达所需的蛋白质。