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公开(公告)号：US5863541A
公开(公告)日：1999-01-26
申请号：US472594
申请日：1995-06-06
IPC分类号： C12N15/00 , A61K35/76 , A61K38/00 , A61K39/00 , A61K48/00 , A61P3/08 , A61P17/00 , A61P37/00 , A61P37/04 , C07K14/015 , C12N7/01 , C12N7/04 , C12N15/864 , A61K39/02 , A61K39/12
CPC分类号： C12N15/86 , C07K14/005 , C12N7/00 , A61K39/00 , A61K48/00 , C12N2710/10344 , C12N2750/14122 , C12N2750/14123 , C12N2750/14143 , Y10S977/804 , Y10S977/84 , Y10S977/918 , Y10S977/92
摘要： The invention relates to the production of AAV capsids which may be used to transfer native or heterologous molecules into appropriate host cells. The capsid proteins can be expressed from a recombinant virus, expression vector, or from a cell line that has stably integrated the AAV capsid genes or coding sequences. The invention further provides for the production of AAV capsids in vitro from the AAV capsid proteins and the construction of packaged capsids in vitro. The invention further provides for the production of AAV capsids that have been genetically engineered to express heterologous epitopes of clinically important antigens to elicit an immune response.
摘要翻译： 本发明涉及可用于将天然或异源分子转移到合适的宿主细胞中的AAV衣壳的制备。 衣壳蛋白可以从重组病毒，表达载体或稳定整合AAV衣壳基因或编码序列的细胞系表达。 本发明进一步提供了从AAV衣壳蛋白体外生产AAV衣壳和体外包装衣壳的构建。 本发明进一步提供已经经遗传工程化以表达临床重要抗原的异源表位以引发免疫应答的AAV衣壳的生产。
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公开(公告)号：US07439065B2
公开(公告)日：2008-10-21
申请号：US11208901
申请日：2005-08-22
IPC分类号： C12N15/63 , C12N15/64 , C12N15/861 , C12N15/86 , C12N15/864 , A61K48/00
CPC分类号： C12N7/00 , C07K14/005 , C12N15/86 , C12N2710/10322 , C12N2710/10343 , C12N2750/14143 , C12N2750/14152
摘要： A method for the production of adeno-associated virus stocks and recombinant adeno-associated virus stocks that are substantially free of contaminating helper virus is described. The method utilizes transfection with helper virus vectors to replace the infection with helper virus used in the conventional method.
摘要翻译： 描述了生产基本上不含污染辅助病毒的腺相关病毒种群和重组腺相关病毒种群的方法。 该方法利用辅助病毒载体的转染来代替常规方法中使用的辅助病毒感染。
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公开(公告)号：US6040183A
公开(公告)日：2000-03-21
申请号：US484515
申请日：1995-06-07
IPC分类号： C12N15/864 , C12N15/86
CPC分类号： C12N15/86 , C12N2750/14143
摘要： A method for the production of adeno-associated virus stocks and recombinant adeno-associated virus stocks that are substantially free of contaminating helper virus is described. The method utilizes transfection with helper virus vectors to replace the infection with helper virus used in the conventional method.
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公开(公告)号：US06204059B1
公开(公告)日：2001-03-20
申请号：US08268430
申请日：1994-06-30
IPC分类号： C12N701
CPC分类号： C12N15/86 , A61K39/00 , A61K48/00 , C07K14/005 , C12N7/00 , C12N2710/10344 , C12N2750/14122 , C12N2750/14123 , C12N2750/14143 , Y10S977/804 , Y10S977/84 , Y10S977/918 , Y10S977/92
摘要： The invention relates to the production of AAV capsids which may be used to transfer native or heterologous molecules into appropriate host cells. The capsid proteins can be expressed from a recombinant virus, expression vector, or from a cell line that has stably integrated the AAV capsid genes or coding sequences. The invention further provides for the production of AAV capsids in vitro from the AAV capsid proteins and the construction of packaged capsids in vitro. The invention further provides for the production of AAV capsids that have been genetically engineered to express heterologous epitopes of clinically important antigens to elicit an immune response.
摘要翻译： 本发明涉及可用于将天然或异源分子转移到合适的宿主细胞中的AAV衣壳的制备。 衣壳蛋白可以从重组病毒，表达载体或稳定整合AAV衣壳基因或编码序列的细胞系表达。 本发明进一步提供了从AAV衣壳蛋白体外生产AAV衣壳和体外包装衣壳的构建。 本发明进一步提供已经经遗传工程化以表达临床重要抗原的异源表位以引发免疫应答的AAV衣壳的生产。
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公开(公告)号：US06951758B2
公开(公告)日：2005-10-04
申请号：US10889105
申请日：2004-07-13
IPC分类号： C07K14/075 , C12N5/08 , C12N5/10 , C12N7/01 , C12N15/864 , C12N15/63 , C12N15/64 , C12N15/861
CPC分类号： C12N7/00 , C07K14/005 , C12N15/86 , C12N2710/10322 , C12N2710/10343 , C12N2750/14143 , C12N2750/14152
摘要： A method for the production of adeno-associated virus stocks and recombinant adeno-associated virus stocks that are substantially free of contaminating helper virus is described. The method utilizes transfection with helper virus vectors to replace the infection with helper virus used in the conventional method.
摘要翻译： 描述了生产基本上不含污染辅助病毒的腺相关病毒种群和重组腺相关病毒种群的方法。 该方法利用辅助病毒载体的转染来代替常规方法中使用的辅助病毒感染。
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公开(公告)号：US06458587B2
公开(公告)日：2002-10-01
申请号：US09813041
申请日：2001-03-21
IPC分类号： C12N15864
CPC分类号： C12N7/00 , C07K14/005 , C12N15/86 , C12N2710/10322 , C12N2710/10343 , C12N2750/14143 , C12N2750/14152
摘要： A method for the production of adeno-associated virus stocks and recombinant adeno-associated virus stocks that are substantially free of contaminating helper virus is described. The method utilizes transfection with helper virus vectors to replace the infection with helper virus used in the conventional method.
摘要翻译： 描述了生产基本上不含污染辅助病毒的腺相关病毒种群和重组腺相关病毒种群的方法。 该方法利用辅助病毒载体的转染来代替常规方法中使用的辅助病毒感染。
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公开(公告)号：US6093570A
公开(公告)日：2000-07-25
申请号：US483108
申请日：1995-06-07
IPC分类号： C07K14/075 , C12N5/08 , C12N5/10 , C12N7/01 , C12N15/864 , C12N15/63 , C12N15/64
CPC分类号： C12N7/00 , C07K14/005 , C12N15/86 , C12N2710/10322 , C12N2710/10343 , C12N2750/14143 , C12N2750/14152
摘要： A method for the production of adeno-associated virus stocks and recombinant adeno-associated virus stocks that are substantially free of contaminating helper virus is described. The method utilizes transfection with helper virus vectors to replace the infection with helper virus used in the conventional method.
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公开(公告)号：US08809058B2
公开(公告)日：2014-08-19
申请号：US12140820
申请日：2008-06-17
IPC分类号： C12N15/63 , C12N15/64 , C12N15/861 , C12N15/86 , C12N15/864 , A61K48/00
CPC分类号： C12N7/00 , C07K14/005 , C12N15/86 , C12N2710/10322 , C12N2710/10343 , C12N2750/14143 , C12N2750/14152
摘要： A method for the production of adeno-associated virus stocks and recombinant adeno-associated virus stocks that are substantially free of contaminating helper virus is described. The method utilizes transfection with helper virus vectors to replace the infection with helper virus used in the conventional method.
摘要翻译： 描述了生产基本上不含污染辅助病毒的腺相关病毒种群和重组腺相关病毒种群的方法。 该方法利用辅助病毒载体的转染来代替常规方法中使用的辅助病毒感染。
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公开(公告)号：US20090004743A1
公开(公告)日：2009-01-01
申请号：US12140820
申请日：2008-06-17
CPC分类号： C12N7/00 , C07K14/005 , C12N15/86 , C12N2710/10322 , C12N2710/10343 , C12N2750/14143 , C12N2750/14152
摘要： A method for the production of adeno-associated virus stocks and recombinant adeno-associated virus stocks that are substantially free of contaminating helper virus is described. The method utilizes transfection with helper virus vectors to replace the infection with helper virus used in the conventional method.
摘要翻译： 描述了生产基本上不含污染辅助病毒的腺相关病毒种群和重组腺相关病毒种群的方法。 该方法利用辅助病毒载体的转染来代替常规方法中使用的辅助病毒感染。
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公开(公告)号：US09169494B2
公开(公告)日：2015-10-27
申请号：US13521448
申请日：2011-01-12
IPC分类号： A01N63/00 , C12N15/864 , C07K14/005 , C12N15/86
CPC分类号： C12N7/00 , C07K14/005 , C12N15/86 , C12N15/8645 , C12N2750/14122 , C12N2750/14143 , C12N2750/14162 , C12N2750/14322 , C12N2750/14352 , C12N2820/60
摘要： This invention relates to modified parvovirus inverted terminal repeats (ITRs) that do not functionally interact with wild-type large Rep proteins, synthetic Rep proteins that functionally interact with the modified ITRs, and methods of using the same for delivery of nucleic acids to a cell or a subject. The modifications provide a novel Rep-ITR interaction that limits vector mobilization, increasing the safety of viral vectors.
摘要翻译： 本发明涉及不与野生型大型Rep蛋白功能性相互作用的修饰的细小病毒反向末端重复序列（ITR），与修饰的ITR功能相互作用的合成Rep蛋白，以及使用该蛋白质将核酸递送至细胞的方法 或主题。 这些修改提供了限制载体动员，增加病毒载体的安全性的新型Rep-ITR相互作用。
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