FLUORESCENT NANOSCOPY DEVICE AND METHOD
    7.
    发明申请
    FLUORESCENT NANOSCOPY DEVICE AND METHOD 审中-公开
    荧光纳米器件及方法

    公开(公告)号:US20150316478A1

    公开(公告)日:2015-11-05

    申请号:US14710214

    申请日:2015-05-12

    IPC分类号: G01N21/64

    摘要: A method for analysis of an object dyed with fluorescent coloring agents. Separately fluorescing visible molecules or nanoparticles are periodically formed in different object parts, the laser produces the oscillation thereof which is sufficient for recording the non-overlapping images of the molecules or nanoparticles and for decoloring already recorded fluorescent molecules, wherein tens of thousands of pictures of recorded individual molecule or nanoparticle images, in the form of stains having a diameter on the order of a fluorescent light wavelength multiplied by a microscope amplification, are processed by a computer for searching the coordinates of the stain centers and building the object image according to millions of calculated stain center co-ordinates corresponding to the co-ordinates of the individual fluorescent molecules or nanoparticles. Two-dimensional and three-dimensional images are provided for proteins, nucleic acids and lipids with different coloring agents.

    摘要翻译: 用荧光着色剂染色的物体的分析方法。 在不同的对象部分中周期性地形成可见分子或纳米颗粒的单独发光,激光产生其振荡,其足以记录分子或纳米颗粒的非重叠图像,并用于对已记录的荧光分子进行脱色,其中数万张图片 记录的单个分子或纳米颗粒图像以直径为荧光波长的倍数乘以显微镜放大的污渍的形式通过计算机进行处理,用于搜索染色中心的坐标并根据百万分数来构建物体图像 对应于各个荧光分子或纳米颗粒的坐标的计算的染色中心坐标。 为不同着色剂的蛋白质,核酸和脂质提供二维和三维图像。