Bead-Based Multiplexed Analytical Methods and Instrumentation
    9.
    发明申请
    Bead-Based Multiplexed Analytical Methods and Instrumentation 审中-公开
    基于珠的多重分析方法和仪器

    公开(公告)号:US20100130369A1

    公开(公告)日:2010-05-27

    申请号:US12596897

    申请日:2008-03-24

    IPC分类号: C40B30/00 C40B50/08

    摘要: Various methods, such as a method of detecting SNPs, involving (a) introducing onto a droplet actuator a solution comprising genomic DNA, extension oligos and suspension array beads; (b) dispensing on the droplet actuator one bead per droplet; (c) cleaving DNA from the bead in each droplet; (d) amplifying the cleaved DNA; (e) detecting SNP signals and barcode signals from the amplified DNA.

    摘要翻译: 各种方法,例如检测SNP的方法,涉及(a)向液滴致动器引入包含基因组DNA,延伸寡核苷酸和悬浮阵列珠的溶液; (b)在液滴致动器上分配每个液滴一个珠; (c)在每个液滴中从珠中切割DNA; (d)扩增切割的DNA; (e)从扩增的DNA检测SNP信号和条形码信号。