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    Method for production of polyketides
    1.
    发明授权
    Method for production of polyketides 有权
    聚酮化合物的生产方法

    公开(公告)号：US07067290B2

    公开(公告)日：2006-06-27

    申请号：US10712897

    申请日：2003-11-12

    申请人： Rika Regentin ,  Ruchir P. Desai

    发明人： Rika Regentin ,  Ruchir P. Desai

    IPC分类号： C12P17/06 ,  C12P7/42 ,  C12P7/26 ,  C12P7/62

    CPC分类号： C12P19/62 ,  C12P17/06

    摘要： A method for the production of a polyketide by fermentation comprising the steps of growing a culture of a polyketide-producing organism at a pH value conducive to cell growth for a time sufficient to generate the producing culture, lowering the pH of the culture to a value conducive to polyketide product stability, continuing the fermentation until a maximal titer of polyketide is achieved, and optionally extracting the polyketide from the culture.

    摘要翻译： 通过发酵生产聚酮化合物的方法包括以下步骤：在有助于细胞生长的pH值下生长足够产生培养物的时间的产生聚酮生物的生物体的培养物，将培养物的pH降低到值 有利于聚酮化合物产品的稳定性，继续发酵，直到达到最大滴度的聚酮化合物，并且任选地从培养物中提取聚酮化合物。

    Production of Isoprenoids
    9.
    发明申请
    Production of Isoprenoids 有权
    异戊二烯的生产

    公开(公告)号：US20130252295A1

    公开(公告)日：2013-09-26

    申请号：US13848045

    申请日：2013-03-20

    申请人： Neil Stephen Renninger ,  Jack Newman ,  Keith Kinkead Reiling ,  Rika Regentin ,  Christopher John Paddon

    发明人： Neil Stephen Renninger ,  Jack Newman ,  Keith Kinkead Reiling ,  Rika Regentin ,  Christopher John Paddon

    IPC分类号： C12P5/00 ,  C12P7/04 ,  C12P7/02 ,  C12P5/02

    CPC分类号： C12P7/00 ,  C12N15/52 ,  C12P5/007 ,  C12P5/026 ,  C12P7/02 ,  C12P7/04

    摘要： Methods for producing an isoprenoid are provided. A plurality of bacterial or fungal host cells is obtained. These cells comprise a heterologous nucleic acid encoding one or more enzymes of a mevalonate pathway for making isopentenyl pyrophosphate. Expression of the one or more enzymes is under control of at least one heterologous transcriptional regulator. The mevalonate pathway comprises (i) an enzyme that condenses acetoacetyl-CoA with acetyl-CoA to form HMG-CoA, (ii) an enzyme that converts HMG-CoA to mevalonate, (iii) an enzyme that phosphorylates mevalonate to mevalonate 5-phosphate, (iv) an enzyme that converts mevalonate 5-phosphate to mevalonate 5-pyrophosphate, and (v) an enzyme that converts mevalonate 5-pyrophosphate to isopentenyl pyrophosphate. The host cells are cultured in a medium under conditions that are suboptimal as compared to conditions. Temperature is maintained at a level below that which would provide for a maximum specific growth rate for the host cells.

    摘要翻译： 提供了制备类异戊二烯的方法。 获得多个细菌或真菌宿主细胞。 这些细胞包含编码一种或多种用于制备异戊烯基焦磷酸的甲羟戊酸途径的酶的异源核酸。 一种或多种酶的表达受至少一种异源转录调节子的控制。 甲羟戊酸途径包括（i）使乙酰乙酰辅酶A与乙酰辅酶A缩合以形成HMG-CoA的酶，（ii）将HMG-CoA转化成甲羟戊酸的酶，（iii）将甲羟戊酸磷酸化成甲羟戊酸磷酸酯5-磷酸的酶 ，（iv）将甲羟戊酸5-磷酸转化为甲基羟基-5-焦磷酸的酶，和（v）将5-焦磷酸甲羟戊酸转化为异戊烯基焦磷酸的酶。 宿主细胞在与条件相比不是最佳的条件下在培养基中培养。 温度保持在低于为宿主细胞提供最大比生长速率的水平。