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公开(公告)号：US06828104B2
公开(公告)日：2004-12-07
申请号：US10219006
申请日：2002-08-14
IPC分类号： C12Q168
CPC分类号： C12Q1/6806 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00722 , C12Q1/6809 , C12Q1/6837 , C40B40/06 , C12Q2565/107 , C12Q2525/155
摘要： This invention provides nucleic acid affinity matrices that bear a large number of different nucleic acid affinity ligands allowing the simultaneous selection and removal of a large number of preselected nucleic acids from the sample. Methods of producing such affinity matrices are also provided. In general the methods involve the steps of a) providing a nucleic acid amplification template array comprising a surface to which are attached at least 50 oligonucleotides having different nucleic acid sequences, and wherein each different oligonucleotide is localized in a predetermined region of said surface, the density of said oligonucleotides is greater than about 60 different oligonucleotides per 1 cm2, and all of said different oligonucleotides have an identical terminal 3′ nucleic acid sequence and an identical terminal 5′ nucleic acid sequence. b) amplifying said multiplicity of oligonucleotides to provide a pool of amplified nucleic acids; and c) attaching the pool of nucleic acids to a solid support.
摘要翻译： 本发明提供了携带大量不同核酸亲和配体的核酸亲和基质，允许同时从样品中选择和除去大量预选核酸。 还提供了生产这种亲和基质的方法。 通常，所述方法包括以下步骤：a）提供核酸扩增模板阵列，其包含与至少50个具有不同核酸序列的寡核苷酸连接的表面，并且其中每个不同的寡核苷酸定位在所述表面的预定区域中， 所述寡核苷酸的密度大于每1cm 2大约60个不同的寡核苷酸，并且所有所述不同寡核苷酸具有相同的末端3'核酸序列和相同的末端5'核酸序列。 b）扩增所述多重寡核苷酸以提供扩增核酸的库; 和c）将核酸池连接到固体支持物上。
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公开(公告)号：US06440677B2
公开(公告)日：2002-08-27
申请号：US09910223
申请日：2001-07-20
IPC分类号： C12Q168
CPC分类号： C12Q1/6806 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00722 , C12Q1/6809 , C12Q1/6837 , C40B40/06 , C12Q2565/107 , C12Q2525/155
摘要： Nucleic acid affinity matrices that bear a large number of different nucleic acid affinity ligands allowing the simultaneous selection and removal of a large number of preselected nucleic acids from the sample. Methods of producing such affinity matrices are also provided. In general the methods involve the steps of a) providing a nucleic acid amplification template array comprising a surface to which are attached at least 50 oligonucleotides having different nucleic acid sequences, and wherein each different oligonucleotide is localized in a predetermined region of the surface, the density of the oligonucleotides is greater than about 60 different oligonucleotides per 1 cm2, and all of the different oligonucleotides have an identical terminal 3′ nucleic acid sequence and an identical terminal 5′ nucleic acid sequence. b) amplifying the multiplicity of oligonucleotides to provide a pool of amplified nucleic acids; and c) attaching the pool of nucleic acids to a solid support.
摘要翻译： 具有大量不同核酸亲和配体的核酸亲和基质，允许从样品中同时选择和除去大量预选核酸。 还提供了生产这种亲和基质的方法。 通常，所述方法包括以下步骤：a）提供核酸扩增模板阵列，其包含与至少50个具有不同核酸序列的寡核苷酸连接的表面，并且其中每个不同的寡核苷酸定位在表面的预定区域中， 每1cm 2寡核苷酸的密度大于约60个不同寡核苷酸，并且所有不同的寡核苷酸具有相同的末端3'核酸序列和相同的末端5'核酸序列。 b）扩增多重寡核苷酸以提供扩增的核酸库; 和c）将核酸池连接到固体支持物上。
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公开(公告)号：US06280950B1
公开(公告)日：2001-08-28
申请号：US09429521
申请日：1999-10-28
IPC分类号： C12Q168
CPC分类号： C12Q1/6806 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00722 , C12Q1/6809 , C12Q1/6837 , C40B40/06 , C12Q2565/107 , C12Q2525/155
摘要： This invention provides nucleic acid affinity matrices that bear a large number of different nucleic acid affinity ligands allowing the simultaneous selection and removal of a large number of preselected nucleic acids from the sample. Methods of producing such affinity matrices are also provided. In general the methods involve the steps of a) providing a nucleic acid amplification template array comprising a surface to which are attached at least 50 oligonucleotides having different nucleic acid sequences, and wherein each different oligonucleotide is localized in a predetermined region of said surface, the density of said oligonucleotides is greater than about 60 different oligonucleotides per 1 cm2, and all of said different oligonucleotides have an identical terminal 3′ nucleic acid sequence and an identical terminal 5′ nucleic acid sequences; b) amplifying said multiplicity of oligonucleotides to provide a pool of amplified nucleic acids; and c) attaching the pool of nucleic acids to a solid support.
摘要翻译： 本发明提供了携带大量不同核酸亲和配体的核酸亲和基质，允许同时从样品中选择和除去大量预选核酸。 还提供了生产这种亲和基质的方法。 通常，所述方法包括以下步骤：a）提供核酸扩增模板阵列，其包含与至少50个具有不同核酸序列的寡核苷酸连接的表面，并且其中每个不同的寡核苷酸定位在所述表面的预定区域中， 所述寡核苷酸的密度大于每平方厘米约60个不同的寡核苷酸，并且所有所述不同的寡核苷酸具有相同的末端3'核酸序列和相同的末端5'核酸序列; b）扩增所述多重寡核苷酸以提供扩增核酸的库; 和c）将核酸池连接到固体支持物上。
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公开(公告)号：US6013440A
公开(公告)日：2000-01-11
申请号：US815395
申请日：1997-03-10
CPC分类号： C12Q1/6806 , C12Q1/6809 , C12Q1/6837 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/0063 , B01J2219/00637 , B01J2219/00641 , B01J2219/00659 , B01J2219/00722 , C40B40/06
摘要： This invention provides nucleic acid affinity matrices that bear a large number of different nucleic acid affinity ligands allowing the simultaneous selection and removal of a large number of preselected nucleic acids from the sample. Methods of producing such affinity matrices are also provided. In general the methods involve the steps of a) providing a nucleic acid amplification template array comprising a surface to which are attached at least 50 oligonucleotides having different nucleic acid sequences, and wherein each different oligonucleotide is localized in a predetermined region of said surface, the density of said oligonucleotides is greater than about 60 different oligonucleotides per 1 cm.sup.2, and all of said different oligonucleotides have an identical terminal 3' nucleic acid sequence and an identical terminal 5' nucleic acid sequence. b) amplifying said multiplicity of oligonucleotides to provide a pool of amplified nucleic acids; and c) attaching the pool of nucleic acids to a solid support.
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公开(公告)号：US07115364B1
公开(公告)日：2006-10-03
申请号：US08510521
申请日：1995-08-02
申请人： Mark Chee , Maureen T. Cronin , Stephen P. A. Fodor , Thomas R. Gingeras , Xiaohua C. Huang , Earl A. Hubbell , Robert J. Lipshutz , Peter E. Lobban , Charles Garrett Miyada , Macdonald S. Morris , Nila Shah , Edward L. Sheldon
发明人： Mark Chee , Maureen T. Cronin , Stephen P. A. Fodor , Thomas R. Gingeras , Xiaohua C. Huang , Earl A. Hubbell , Robert J. Lipshutz , Peter E. Lobban , Charles Garrett Miyada , Macdonald S. Morris , Nila Shah , Edward L. Sheldon
CPC分类号： C12Q1/6869 , C12Q1/6886 , Y02A50/54
摘要： The invention provides chips of immobilized probes, and methods employing the chips, for comparing a reference polynucleotide sequence of known sequence with a target sequence showing substantial similarity with the reference sequence, but differing in the presence of e.g., mutations.
摘要翻译： 本发明提供了固定化探针的芯片，以及使用该芯片的方法，用于将已知序列的参考多核苷酸序列与显示与参考序列基本相似的靶序列进行比较，但在例如突变的存在下不同。
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6.发明申请Capturing sequences adjacent to type IIs restriction sites for genomic library mapping 审中-公开捕获与II型限制性位点相邻的序列用于基因组文库作图公开(公告)号：US20120258868A1
公开(公告)日：2012-10-11
申请号：US13159626
申请日：2011-06-14
CPC分类号： C12Q1/683 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6874 , C12Q2565/501 , C12Q2600/156 , C12Q2525/191 , C12Q2521/313 , C12Q2525/179 , C12Q2525/161 , C12Q2525/155 , C12Q2525/143 , C12Q2525/131
摘要： The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation.
摘要翻译： 本发明涉及使用II型限制性内切核酸酶对多核苷酸序列中的遗传标记进行测序和鉴定的新方法。 本文描述的方法导致捕获和确定位于II型限制性位点附近的特异性寡核苷酸序列。 所得序列可用作用于遗传图谱，筛选和操纵的有效标记。
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7.发明申请Capturing Sequences Adjacent to type-IIs restriction sites for Genomic Library Mapping 失效捕获序列与基因组图谱绘制的II型限制性位点相邻公开(公告)号：US20100261616A1
公开(公告)日：2010-10-14
申请号：US12272680
申请日：2008-11-17
CPC分类号： C12Q1/683 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6874 , C12Q2565/501 , C12Q2600/156 , C12Q2525/191 , C12Q2521/313 , C12Q2525/179 , C12Q2525/161 , C12Q2525/155 , C12Q2525/143 , C12Q2525/131
摘要： The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation.
摘要翻译： 本发明涉及使用II型限制性内切核酸酶对多核苷酸序列中的遗传标记进行测序和鉴定的新方法。 本文描述的方法导致“捕获”和确定与II型限制性位点相邻的特定寡核苷酸序列。 所得序列可用作用于遗传图谱，筛选和操纵的有效标记。
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8.发明授权Methods for analyzing nucleic acids using a type IIs restriction endonuclease 失效使用II型限制性内切核酸酶分析核酸的方法公开(公告)号：US06509160B1
公开(公告)日：2003-01-21
申请号：US09940868
申请日：2001-08-27
IPC分类号： C12Q168
CPC分类号： C12Q1/683 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6874 , C12Q2565/501 , C12Q2600/156 , C12Q2525/191 , C12Q2521/313 , C12Q2525/179 , C12Q2525/161 , C12Q2525/155 , C12Q2525/143 , C12Q2525/131
摘要： The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation.
摘要翻译： 本发明涉及使用II型限制性内切核酸酶对多核苷酸序列中的遗传标记进行测序和鉴定的新方法。 本文描述的方法导致“捕获”和确定与II型限制性位点相邻的特定寡核苷酸序列。 所得序列可用作用于遗传图谱，筛选和操纵的有效标记。
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9.发明授权Nucleic acid adapters containing a type IIs restriction site and methods of using the same 有权含有II型限制性位点的核酸衔接子及其使用方法公开(公告)号：US06291181B1
公开(公告)日：2001-09-18
申请号：US09412246
申请日：1999-10-05
IPC分类号： C12Q168
CPC分类号： C12Q1/683 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6874 , C12Q2565/501 , C12Q2600/156 , C12Q2525/191 , C12Q2521/313 , C12Q2525/179 , C12Q2525/161 , C12Q2525/155 , C12Q2525/143 , C12Q2525/131
摘要： The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the “capturing” and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation.
摘要翻译： 本发明涉及使用II型限制性内切核酸酶对多核苷酸序列中的遗传标记进行测序和鉴定的新方法。 本文描述的方法导致“捕获”和确定与II型限制性位点相邻的特定寡核苷酸序列。 所得序列可用作用于遗传图谱，筛选和操纵的有效标记。
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10.发明授权Nucleic acid adapters containing a type IIs restriction site and methods of using the same 失效含有II型限制性位点的核酸衔接子及其使用方法
公开(公告)号：US6027894A
公开(公告)日：2000-02-22
申请号：US8094
申请日：1998-01-16
IPC分类号： C12Q1/68
CPC分类号： C12Q1/683 , C12Q1/6806 , C12Q1/6809 , C12Q1/6827 , C12Q1/6855 , C12Q1/6869 , C12Q1/6874 , C12Q2565/501 , C12Q2600/156
摘要： The present invention relates to novel methods for sequencing and mapping genetic markers in polynucleotide sequences using Type-IIs restriction endonucleases. The methods herein described result in the "capturing" and determination of specific oligonucleotide sequences located adjacent to Type-IIs restriction sites. The resulting sequences are useful as effective markers for use in genetic mapping, screening and manipulation.
摘要翻译： 本发明涉及使用II型限制性内切核酸酶对多核苷酸序列中的遗传标记进行测序和鉴定的新方法。 本文描述的方法导致“捕获”和确定与II型限制性位点相邻的特定寡核苷酸序列。 所得序列可用作用于遗传图谱，筛选和操纵的有效标记。
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