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    Cryopreservation of Haptenized Tumor Cells
    5.
    发明申请
    Cryopreservation of Haptenized Tumor Cells 审中-公开
    Haptenized肿瘤细胞的冷冻保存

    公开(公告)号：US20140113371A1

    公开(公告)日：2014-04-24

    申请号：US13888024

    申请日：2013-05-06

    申请人： David Berd ,  Lorne F. Erdile ,  Margalit B. Mokyr

    发明人： David Berd ,  Lorne F. Erdile ,  Margalit B. Mokyr

    IPC分类号： A01N1/02

    CPC分类号： A01N1/0221 ,  A01N1/02

    摘要： A method of preserving haptenized tumor cells is described. The method employs a freezing medium containing an effective amount of sucrose and human serum albumin in an isotonic buffered saline solution. Cryogenically preserving haptenized cells in such a medium has been found to maintain the integrity of the tumor cells during storage. The haptenized tumor cells also retain cell-associated antigens and haptens, and are as immunogenic, i.e., capable of inducing immunotherapeutic response, as fresh vaccine in a mouse model of metastatic disease. In a specific embodiment, haptenized cells are exposed to a solution of 8% sucrose, 10% human serum albumin in Hank's buffered solution, and then frozen to −80° C. overnight and then stored in a liquid nitrogen freezer. Methods of storing haptenized tumor cells and compositions are also provided.

    摘要翻译： 描述了保留半抗原的肿瘤细胞的方法。 该方法在等渗缓冲盐水溶液中使用含有有效量的蔗糖和人血清白蛋白的冷冻介质。 已经发现在这种培养基中低温保存半抗原细胞在保存期间维持肿瘤细胞的完整性。 半抗原的肿瘤细胞还保留细胞相关抗原和半抗原，并且作为免疫原性，即能够诱导免疫治疗应答，作为转移性疾病的小鼠模型中的新鲜疫苗。 在一个具体实施方案中，将半抗原细胞暴露于Hank's缓冲溶液中的8％蔗糖，10％人血清白蛋白的溶液中，然后冷冻至-80℃过夜，然后储存在液氮冷冻器中。 还提供了存储半抗原化肿瘤细胞和组合物的方法。

    Cryopreservation of haptenized tumor cells
    6.
    发明授权
    Cryopreservation of haptenized tumor cells 失效
    半抗原肿瘤细胞的冷冻保存

    公开(公告)号：US08435784B2

    公开(公告)日：2013-05-07

    申请号：US11762409

    申请日：2007-06-13

    申请人： David Berd ,  Lorne F. Erdile ,  Margalit B. Mokyr

    发明人： David Berd ,  Lorne F. Erdile ,  Margalit B. Mokyr

    IPC分类号： A01N1/00 ,  A01N1/02 ,  C12N5/00 ,  C12N5/02 ,  C12N5/07 ,  C12N5/16 ,  G01N33/574

    CPC分类号： A01N1/0221 ,  A01N1/02

    摘要： A method of preserving haptenized tumor cells is described. The method employs a freezing medium containing an effective amount of sucrose and human serum albumin in an isotonic buffered saline solution. Cryogenically preserving haptenized cells in such a medium has been found to maintain the integrity of the tumor cells during storage. The haptenized tumor cells also retain cell-associated antigens and haptens, and are as immunogenic, i.e., capable of inducing immunotherapeutic response, as fresh vaccine in a mouse model of metastatic disease. In a specific embodiment, haptenized cells are exposed to a solution of 8% sucrose, 10% human serum albumin in Hank's buffered solution, and then frozen to −80° C. overnight and then stored in a liquid nitrogen freezer. Methods of storing haptenized tumor cells and compositions are also provided.

    摘要翻译： 描述了保留半抗原的肿瘤细胞的方法。 该方法在等渗缓冲盐水溶液中使用含有有效量的蔗糖和人血清白蛋白的冷冻介质。 已经发现在这种培养基中低温保存半抗原细胞在保存期间维持肿瘤细胞的完整性。 半抗原的肿瘤细胞还保留细胞相关抗原和半抗原，并且作为免疫原性，即能够诱导免疫治疗应答，作为转移性疾病的小鼠模型中的新鲜疫苗。 在一个具体实施方案中，将半抗原细胞暴露于Hank's缓冲溶液中的8％蔗糖，10％人血清白蛋白的溶液中，然后冷冻至-80℃过夜，然后储存在液氮冷冻器中。 还提供了存储半抗原化肿瘤细胞和组合物的方法。