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公开(公告)号:US08945835B2
公开(公告)日:2015-02-03
申请号:US13444654
申请日:2012-04-11
申请人: Eric Hans Vermaas , Graham John Worsley , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , Geoffrey Paul Smith , Vincent Peter Smith , Tobias William Barr Ost , David Bentley
发明人: Eric Hans Vermaas , Graham John Worsley , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , Geoffrey Paul Smith , Vincent Peter Smith , Tobias William Barr Ost , David Bentley
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6874 , C12Q1/6869 , C12Q2565/518 , C12Q2537/149
摘要: The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.
摘要翻译: 本发明提供了双链多核苷酸模板的成对测序的方法,该方法导致多核苷酸模板的两个不同和分离的区域中的核苷酸序列的顺序测定。
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公开(公告)号:US20110009276A1
公开(公告)日:2011-01-13
申请号:US12223759
申请日:2007-02-08
申请人: Eric Hans Vermaas , Graham John Worsley , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , Geoffrey Paul Smith , Vincent Peter Smith , Tobias Willaim Barr Ost , David Bentley
发明人: Eric Hans Vermaas , Graham John Worsley , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , Geoffrey Paul Smith , Vincent Peter Smith , Tobias Willaim Barr Ost , David Bentley
IPC分类号: C40B30/00
CPC分类号: C12Q1/6874 , C12Q1/6869 , C12Q2565/518 , C12Q2537/149
摘要: The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.
摘要翻译: 本发明提供了双链多核苷酸模板的成对测序的方法,该方法导致多核苷酸模板的两个不同和分离的区域中的核苷酸序列的顺序测定。
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公开(公告)号:US20130281306A1
公开(公告)日:2013-10-24
申请号:US13881320
申请日:2011-10-26
申请人: Roberto Rigatti , Jonathan Mark Boutell , Jason Richard Betley , Niall Anthony Gormley , Mostafa Ronaghi , Dirk Evers
发明人: Roberto Rigatti , Jonathan Mark Boutell , Jason Richard Betley , Niall Anthony Gormley , Mostafa Ronaghi , Dirk Evers
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6874 , C12Q2565/514 , C12Q2537/155 , C12Q2525/186 , C12Q2525/185 , C12Q2537/113 , C12Q2525/204
摘要: The present technology relates to molecular sciences, such as genomics. More particularly, the present technology relates to nucleic acid sequencing.
摘要翻译: 本技术涉及分子科学,如基因组学。 更具体地,本技术涉及核酸测序。
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公开(公告)号:US20120053063A1
公开(公告)日:2012-03-01
申请号:US13219422
申请日:2011-08-26
IPC分类号: C40B20/00
CPC分类号: C12Q1/6874 , C12Q2535/119 , C12Q2535/122 , C12Q2565/513 , C12Q2565/543
摘要: Provided herein is a method for sequencing a polynucleotide molecules. The method includes the steps of providing a plurality of polynucleotide molecules attached to a surface, wherein a first portion of each polynucleotide molecule is attached to a first location of the surface and a second portion of each polynucleotide molecule is attached to a second location of the surface, the relative proximity of the first and second locations being correlated with the probability that the first and second portions are paired, separating the first and second portions of the polynucleotide molecules on the surface, determining the sequences of the first and second portions of the polynucleotide molecules and comparing the relative proximities and the sequences to determine which first and second portions are paired and to determine the sequence of the target polynucleotide molecules.
摘要翻译: 本文提供了用于测序多核苷酸分子的方法。 该方法包括提供连接到表面的多个多核苷酸分子的步骤,其中每个多核苷酸分子的第一部分连接到该表面的第一位置,并且每个多核苷酸分子的第二部分连接到该多核苷酸分子的第二位置 表面,第一和第二位置的相对接近度与第一和第二部分配对的概率相关,分离表面上的多核苷酸分子的第一和第二部分,确定第一和第二部分的序列 多核苷酸分子,并比较相对接近度和序列以确定哪个第一和第二部分配对并确定靶多核苷酸分子的序列。
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公开(公告)号:US09029103B2
公开(公告)日:2015-05-12
申请号:US13219422
申请日:2011-08-26
CPC分类号: C12Q1/6874 , C12Q2535/119 , C12Q2535/122 , C12Q2565/513 , C12Q2565/543
摘要: Provided herein is a method for sequencing a polynucleotide molecules. The method includes the steps of providing a plurality of polynucleotide molecules attached to a surface, wherein a first portion of each polynucleotide molecule is attached to a first location of the surface and a second portion of each polynucleotide molecule is attached to a second location of the surface, the relative proximity of the first and second locations being correlated with the probability that the first and second portions are paired, separating the first and second portions of the polynucleotide molecules on the surface, determining the sequences of the first and second portions of the polynucleotide molecules and comparing the relative proximities and the sequences to determine which first and second portions are paired and to determine the sequence of the target polynucleotide molecules.
摘要翻译: 本文提供了用于测序多核苷酸分子的方法。 该方法包括提供连接到表面的多个多核苷酸分子的步骤,其中每个多核苷酸分子的第一部分连接到该表面的第一位置,并且每个多核苷酸分子的第二部分连接到该多核苷酸分子的第二位置 表面,第一和第二位置的相对接近度与第一和第二部分配对的概率相关,分离表面上的多核苷酸分子的第一和第二部分,确定第一和第二部分的序列 多核苷酸分子,并比较相对接近度和序列以确定哪个第一和第二部分配对并确定靶多核苷酸分子的序列。
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公开(公告)号:US08753816B2
公开(公告)日:2014-06-17
申请号:US13881320
申请日:2011-10-26
申请人: Roberto Rigatti , Jonathan Mark Boutell , Jason Richard Betley , Niall Anthony Gormley , Mostafa Ronaghi , Dirk Evers
发明人: Roberto Rigatti , Jonathan Mark Boutell , Jason Richard Betley , Niall Anthony Gormley , Mostafa Ronaghi , Dirk Evers
CPC分类号: C12Q1/6874 , C12Q2565/514 , C12Q2537/155 , C12Q2525/186 , C12Q2525/185 , C12Q2537/113 , C12Q2525/204
摘要: The present technology relates to molecular sciences, such as genomics. More particularly, the present technology relates to nucleic acid sequencing.
摘要翻译: 本技术涉及分子科学,如基因组学。 更具体地,本技术涉及核酸测序。
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公开(公告)号:US20130244886A1
公开(公告)日:2013-09-19
申请号:US13825956
申请日:2011-09-28
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6874 , C12Q1/6806 , C12Q2525/101 , C12Q2525/186 , C12Q2525/191 , C12Q2565/543 , C12Q2565/537 , C12Q2535/122 , C12Q2523/301 , C12Q2521/319
摘要: Disclosed herein are compositions and methods for sequencing nucleic acids.
摘要翻译: 本文公开了用于测序核酸的组合物和方法。
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公开(公告)号:US20130165327A1
公开(公告)日:2013-06-27
申请号:US13444654
申请日:2012-04-11
申请人: Eric Hans Vermaas , Graham John Worsley , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , Geoffrey Paul Smith , Vincent Peter Smith , Tobias William Barr Ost , David Bentley
发明人: Eric Hans Vermaas , Graham John Worsley , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , Geoffrey Paul Smith , Vincent Peter Smith , Tobias William Barr Ost , David Bentley
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6874 , C12Q1/6869 , C12Q2565/518 , C12Q2537/149
摘要: The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.
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公开(公告)号:US08759038B2
公开(公告)日:2014-06-24
申请号:US13825956
申请日:2011-09-28
CPC分类号: C12Q1/6874 , C12Q1/6806 , C12Q2525/101 , C12Q2525/186 , C12Q2525/191 , C12Q2565/543 , C12Q2565/537 , C12Q2535/122 , C12Q2523/301 , C12Q2521/319
摘要: Disclosed herein are compositions and methods for sequencing nucleic acids.
摘要翻译: 本文公开了用于测序核酸的组合物和方法。
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公开(公告)号:US08192930B2
公开(公告)日:2012-06-05
申请号:US12223759
申请日:2007-02-08
申请人: Eric Hans Vermaas , Graham John Worsley , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , Geoffrey Paul Smith , Vincent Peter Smith , Tobias William Barr Ost , David Bentley
发明人: Eric Hans Vermaas , Graham John Worsley , Jonathan Mark Boutell , Colin Lloyd Barnes , Roberto Rigatti , Niall Anthony Gormley , Geoffrey Paul Smith , Vincent Peter Smith , Tobias William Barr Ost , David Bentley
IPC分类号: C12Q1/68
CPC分类号: C12Q1/6874 , C12Q1/6869 , C12Q2565/518 , C12Q2537/149
摘要: The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.
摘要翻译: 本发明提供了双链多核苷酸模板的成对测序的方法,该方法导致多核苷酸模板的两个不同和分离的区域中的核苷酸序列的顺序测定。
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