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    7 α-hydroxysteroid dehydrogenase knockout mutants and use therefor
    2.
    发明授权
    7 α-hydroxysteroid dehydrogenase knockout mutants and use therefor 有权
    7α-羟基类固醇脱氢酶敲除突变体并用于此

    公开(公告)号：US09096825B2

    公开(公告)日：2015-08-04

    申请号：US13699239

    申请日：2011-05-27

    申请人： Rolf Schmid ,  Michael Braun ,  Luo Liu ,  Arno Aigner ,  Dirk Weuster-Botz

    发明人： Rolf Schmid ,  Michael Braun ,  Luo Liu ,  Arno Aigner ,  Dirk Weuster-Botz

    IPC分类号： C12P33/00 ,  C12N9/04 ,  C12N1/20 ,  C12N15/00 ,  C12N15/87 ,  C07K14/245 ,  C12P33/06

    CPC分类号： C12N9/0006 ,  C07K14/245 ,  C12P33/00 ,  C12P33/06 ,  C12Y101/01159

    摘要： The invention relates to 12 α-hydroxysteroid dehydrogenases, nucleic acid sequences coding for the same, expression cassettes and vectors, recombinant microorganisms containing the corresponding coding nucleic acid sequences, a method for producing said 12 α-hydroxysteroid dehydrogenases, a method for enzymatic oxidation of 12 α-hydroxysteroids using said enzyme, a method for enzymatic reduction of 12-ketosteroids using said enzyme, a method for qualitative or quantitative determination of 12-ketosteroids and/or 12α-hydroxysteroids using said 12α-hydroxysteroid dehydrogenases and a method for production of ursodesoxycholic acid, comprising the enzyme-catalyzed cholic acid oxidation using said 12 α-hydroxysteroid dehydrogenases.

    摘要翻译： 本发明涉及12种α-羟基类固醇脱氢酶，编码相同的核酸序列，表达盒和载体，含有相应的编码核酸序列的重组微生物，所述12种α-羟基类固醇脱氢酶的制备方法， 使用所述酶的12种α-羟基类固醇，使用所述酶酶促还原12-酮类固醇的方法，使用所述12α-羟基类固醇脱氢酶定性或定量测定12-酮类和/或12α-羟基类固醇的方法及其制备方法 熊去氧胆酸，其包含使用所述12α-羟基类固醇脱氢酶的酶催化的胆酸氧化。

    Device for the series cultivation of micro-organisms or cells in gasified liquid columns
    4.
    发明授权
    Device for the series cultivation of micro-organisms or cells in gasified liquid columns 失效
    用于气化液柱中微生物或细胞的系列培养的装置

    公开(公告)号：US6063618A

    公开(公告)日：2000-05-16

    申请号：US11318

    申请日：1998-02-04

    申请人： Dirk Weuster-Botz ,  Jutta Altenbach-Rehm

    发明人： Dirk Weuster-Botz ,  Jutta Altenbach-Rehm

    IPC分类号： C12M1/24 ,  C12M3/06 ,  C12M3/00

    CPC分类号： C12M23/08 ,  C12M23/24 ,  C12M29/06 ,  C12M37/00

    摘要： A reaction apparatus has a plurality of reaction flasks whose bottoms can communicate with a sterile gas source and are provided with porous filter plates which permit the gas to flow into the vessel but prevent the liquid from passing out through the plates. The culture flasks are received in pockets on an upper plate of a double-bottom structure which can communicate through the upper plate between a space within the double bottom and the bottom of the culture flasks. The spiral gas feed is connected to the double-bottom space.

    摘要翻译： PCT No.PCT / DE96 / 01485 Sec。 371日期：1998年2月4日 102（e）1998年2月4日PCT PCT 1996年8月3日PCT公布。 公开号WO97 / 06239 日期1997年2月20日反应装置具有多个反应烧瓶，其底部可与无菌气体源连通并且设置有允许气体流入容器但防止液体通过板的多孔过滤板。 将培养瓶接纳在双底结构的上板上的口袋中，该双层底部结构的上板可以在双层底部之间的空间和培养瓶的底部之间通过上板连通。 螺旋气体进料连接到双底空间。

    CAVITATION REACTOR
    5.
    发明申请
    CAVITATION REACTOR 审中-公开

    公开(公告)号：US20120222744A1

    公开(公告)日：2012-09-06

    申请号：US13387858

    申请日：2010-06-09

    申请人： Dominik Maslak ,  Dirk Weuster-Botz

    发明人： Dominik Maslak ,  Dirk Weuster-Botz

    IPC分类号： B01F3/04 ,  F17D1/00

    CPC分类号： B01J19/006 ,  B01F3/0807 ,  B01F5/0646 ,  B01F5/0652 ,  B01F5/0682 ,  B01F5/0688 ,  B01J19/008 ,  B01J19/26 ,  C02F1/34 ,  Y10T137/0318

    摘要： Cavitation reactor for hydrodynamic generation of homogeneous, oscillating cavitation bubbles in a fluid, including, while forming a flow duct and arranged one behind the other in the direction of flow of the fluid, an acceleration section designed to increase a flow velocity, a diaphragm arranged transversely to the direction of flow with a plurality of micro-passages designed for generating cavitation bubbles, where at least 10 micro-passages per cm2 are provided over an entire flow cross-sectional surface defined on an oncoming flow side of the diaphragm, a stabilization section designed for stabilizing an oscillation of cavitation bubbles, and a collapse section with at least one widening of a flow cross-sectional surface of the flow duct in the direction of flow.

    摘要翻译： 气蚀反应器，用于在流体中产生均匀的振荡空化气泡，其包括在形成流动管道的同时沿着流体的流动方向彼此排列，设计成增加流速的加速部分， 横向于流动方向，具有多​​个设计用于产生空化气泡的微通道，其中在每个cm 2处至少10微通道设置在限定在隔膜的迎面流动侧上的整个流动横截面上，稳定 设计用于稳定气泡的振动的截面，以及在流动方向上具有流动管道的流动横截面的至少一个加宽的塌陷部分。

    Sample system for fluid samples
    6.
    发明授权
    Sample system for fluid samples 有权
    流体样品样品系统

    公开(公告)号：US07958793B2

    公开(公告)日：2011-06-14

    申请号：US12089829

    申请日：2006-10-13

    申请人： Julia Hiller ,  Karl-Heinz Zacher ,  Dirk Weuster-Botz ,  Andreas Kusterer

    发明人： Julia Hiller ,  Karl-Heinz Zacher ,  Dirk Weuster-Botz ,  Andreas Kusterer

    IPC分类号： G01N1/10

    CPC分类号： G01N1/10 ,  C12M33/04 ,  G01N1/22 ,  G01N35/1079 ,  G01N2001/2078

    摘要： Disclosed herein are embodiments of a sampling system for fluid samples having sample receiving vessel for receiving the sample, which is sealed by a septum and holder for the sample receiving vessel for receiving and holding the sample receiving vessel and also a sample probe for dipping into a fluid volume and for taking a fluid sample from the fluid volume, the sample probe being configured as a hollow volume which, at one end, has a first opening for introducing the holder and the sample receiving vessel and, at another end, is sealed by a first valve which is provided with a hollow needle which projects into the interior of the sample probe said valve being able to be opened by contact with at least one of the group comprising the holder and the sample receiving vessel and then connecting the exterior of the sample probe to the inner volume of the hollow needle.

    摘要翻译： 本文公开了一种用于流体样品的采样系统的实施例，其具有用于接收样品的样品接收容器，其由用于接收和保持样品接收容器的样品接收容器的隔膜和保持器密封，以及用于浸入到样品接收容器中的样品探针 流体体积，并且用于从流体体积中取出流体样品，样品探针被构造为中空体积，其一端具有用于引入保持器和样品接收容器的第一开口，并且在另一端由 第一阀，其设置有中空针，该中空针突出到样品探针的内部，所述阀能够通过与包括保持器和样品接收容器的组中的至少一个接触而打开，然后连接 样品探针到中空针的内部体积。

    Sample System For Fluid Samples
    7.
    发明申请
    Sample System For Fluid Samples 有权
    流体样品样品系统

    公开(公告)号：US20090038419A1

    公开(公告)日：2009-02-12

    申请号：US12089829

    申请日：2006-10-13

    申请人： Julia Hiller ,  Karl-Heinz Zacher ,  Dirk Weuster-Botz ,  Andreas Kusterer

    发明人： Julia Hiller ,  Karl-Heinz Zacher ,  Dirk Weuster-Botz ,  Andreas Kusterer

    IPC分类号： G01N1/10

    CPC分类号： G01N1/10 ,  C12M33/04 ,  G01N1/22 ,  G01N35/1079 ,  G01N2001/2078

    摘要： The present invention relates to a sampling system for fluid samples having sample receiving vessel for receiving the sample, which is sealed by a septum and holder for the sample receiving vessel for receiving and holding the sample receiving vessel and also a sample probe for dipping into a fluid volume and for taking a fluid sample from the fluid volume, the sample probe being configured as a hollow volume which, at one end, has a first opening for introducing the holder and the sample receiving vessel and, at another end, is sealed by a first valve which is provided with a hollow needle which projects into the interior of the sample probe said valve being able to be opened by contact with the holder and/or with the sample receiving vessel and then connecting the exterior of the sample probe to the inner volume of the hollow needle.

    摘要翻译： 本发明涉及一种用于流体样品的采样系统，其具有用于接收样品的样品接收容器，该样品接收容器由用于接收和保持样品接收容器的样品接收容器的隔膜和保持器密封，以及用于浸入 流体体积，并且用于从流体体积中取出流体样品，样品探针被构造为中空体积，其一端具有用于引入保持器和样品接收容器的第一开口，并且在另一端由 第一阀，其设置有中空针，其突出到样品探针的内部，所述阀能够通过与保持器和/或样品接收容器接触而打开，然后将样品探针的外部连接到 中空针的内部容积。

    Process for the separation of organic substances from an aqueous mixture
    8.
    发明授权
    Process for the separation of organic substances from an aqueous mixture 失效
    从含水混合物中分离有机物质的方法

    公开(公告)号：US06793825B2

    公开(公告)日：2004-09-21

    申请号：US09984290

    申请日：2001-10-29

    申请人： Dietrich H. Maass ,  Dirk Weuster-Botz ,  Ralf Takors ,  Christian Wandrey ,  Holger Paschold

    发明人： Dietrich H. Maass ,  Dirk Weuster-Botz ,  Ralf Takors ,  Christian Wandrey ,  Holger Paschold

    IPC分类号： C02F144

    CPC分类号： B01D61/246 ,  C07B63/00 ,  C07C227/40

    摘要： The present invention relates to a process for separating from an aqueous mixture one or more organic substances containing at least one positively charged and/or chargeable nitrogenous group by means of extraction via at least one porous membrane, wherein use is made of an extraction agent which contains at least partially relatively long-chain organic compounds and at least one liquid cation exchanger, and of a membrane that is wettable by either the aqueous mixture or by the extraction agent. In particular, the invention relates to a process for separating from an aqueous mixture one or more organic substances containing at least one positively charged and/or chargeable nitrogenous group by means of extraction via at least one porous membrane, wherein the aqueous mixture is drawn from a reservoir 1, led across a first porous membrane 3 which is wettable by either the aqueous mixture or by an extraction agent 5 which contains at least partially relatively long-chain organic compounds and at least one liquid cation exchanger, extracted with the extraction agent 5, the aqueous retentate is returned to the reservoir, the extracted organic substances are led across a second porous membrane 6 which is wettable by either the aqueous mixture or by the extraction agent and there re-extracted into an aqueous phase 7.

    摘要翻译： 本发明涉及一种通过至少一种多孔膜从含水混合物中分离含有至少一个带正电和/或带电荷的含氮基团的有机物质的方法，其中使用提取剂 含有至少部分相对较长链的有机化合物和至少一种液体阳离子交换剂，以及可由含水混合物或萃取剂润湿的膜。 特别地，本发明涉及一种通过至少一种多孔膜从含水混合物中分离含有至少一个带正电和/或带电荷的含氮基团的有机物质的方法，其中将含水混合物从 一个储存器1，穿过第一多孔膜3，该第一多孔膜3可由含水混合物或提取剂5润湿，该提取剂含有至少部分相对较长链的有机化合物和至少一种液体阳离子交换剂，用萃取剂5萃取 ，水性渗余物返回储存器，萃取的有机物质被引导通过第二多孔膜6，第二多孔膜6可由含水混合物或萃取剂润湿，并再次萃取到水相7中。

    Method For The Microbial Production Of A Body Containing Cellulose
    9.
    发明申请
    Method For The Microbial Production Of A Body Containing Cellulose 审中-公开
    含有纤维素的微生物生产方法

    公开(公告)号：US20110319607A1

    公开(公告)日：2011-12-29

    申请号：US13127066

    申请日：2009-11-09

    申请人： Gunter Bertholdt ,  Dirk Weuster-Botz ,  Michael Hofinger ,  Katharina Seiffe

    发明人： Gunter Bertholdt ,  Dirk Weuster-Botz ,  Michael Hofinger ,  Katharina Seiffe

    IPC分类号： C08B37/00 ,  C12M1/12 ,  C12P19/04

    CPC分类号： C12P19/04 ,  C12M21/08 ,  C12M25/02

    摘要： The invention relates to a method for producing a body (7) comprising cellulose with the help of cellulose-producing organisms, which are supplied with a nutrient solution. A particularly uniform structure and density of the cellulose bodies can be achieved when a vessel (1) having a membrane (4) is supplied, said membrane being permeable to the nutrient solution and is substantially impermeable to the organisms, wherein the organisms are cultivated on one side of the membrane (4), where a gas atmosphere prevails, and the nutrient solution is arranged on the other side of the membrane and passes through the membrane (4) in the direction of the organisms, to supply them.

    摘要翻译： 本发明涉及一种生产包含纤维素的身体（7）的方法，所述纤维素借助于生产有纤维素的生物体，其被供应营养液。 当提供具有膜（4）的容器（1）时，可以实现纤维素体的特别均匀的结构和密度，所述膜对营养液是可渗透的并且对生物体是基本上不可渗透的，其中生物在 膜（4）的一侧，其中气体气氛占优势，并且营养液布置在膜的另一侧上并沿生物体的方向穿过膜（4）以供给它们。