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    DOWN-REGULATING GENE EXPRESSION IN INSECT PESTS
    4.
    发明申请
    DOWN-REGULATING GENE EXPRESSION IN INSECT PESTS 有权
    下调调控基因表达

    公开(公告)号：US20130291188A1

    公开(公告)日：2013-10-31

    申请号：US13881792

    申请日：2011-10-27

    申请人： Thierry Bogaert ,  Romaan Raemaekers ,  Yann Naudet

    发明人： Thierry Bogaert ,  Romaan Raemaekers ,  Yann Naudet

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8286 ,  C12N15/113 ,  C12N2310/14 ,  C12N2310/51 ,  C12N2310/531 ,  C12N2330/51

    摘要： The present invention relates to the prevention and/or control of infestation by insect pest species. In particular, the invention relates to down-regulation of expression of target genes in insect pests using interfering ribonucleic acid (RNA) molecules. Also described are transgenic plants that (i) express or are capable of expressing interfering RNAs of the invention and (ii) are resistant to infestation by insect pest species. Compositions containing the interfering RNAs of the invention are also provided.

    摘要翻译： 本发明涉及防治和/或防治害虫物种侵染。 特别地，本发明涉及使用干扰核糖核酸（RNA）分子对害虫中靶基因表达的下调。 还描述了（i）表达或能够表达本发明的干扰RNA的转基因植物，和（ii）对昆虫害虫侵染的抗性。 还提供了含有本发明的干扰RNA的组合物。

    Characterisation of gene function using double stranded RNA inhibition
    6.
    发明授权
    Characterisation of gene function using double stranded RNA inhibition 有权
    使用双链RNA抑制表征基因功能

    公开(公告)号：US08114980B2

    公开(公告)日：2012-02-14

    申请号：US10826522

    申请日：2004-04-16

    申请人： Geert Plaetinck ,  Christ Platteeuw ,  Katharine Mortier ,  Thierry Bogaert

    发明人： Geert Plaetinck ,  Christ Platteeuw ,  Katharine Mortier ,  Thierry Bogaert

    IPC分类号： C07H21/04 ,  C12N15/11 ,  G01N33/569 ,  C12Q1/68

    CPC分类号： C12N15/1079 ,  C12N15/1093 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8279 ,  C12N15/8285 ,  C12N15/86 ,  C12N2795/10243 ,  C12N2800/40 ,  C12N2830/002 ,  C12N2830/008 ,  C12N2830/42 ,  C12Q1/68 ,  Y02A40/164

    摘要： There is provided a method of identifying DNA responsible for conferring a particular phenotype in a cell which method comprises a) constructing a cDNA or genomic library of the DNA of the cell in a suitable vector in an orientation relative to a promoter(s) capable of initiating transcription of the cDNA or DNA to double stranded (ds) RNA upon binding of an appropriate transcription factor to the promoter(s), b) introducing the library into one or more of the cells comprising the transcription factor, and c) identifying and isolating a particular phenotype of the cell comprising the library and identifying the DNA or cDNA fragment from the library responsible for conferring the phenotype. Using this technique it is also possible to assign function to a known DNA sequence by a) identifying a homologue(s) of the DNA sequence in a cell, b) isolating the relevant DNA homologue(s) or a fragment thereof from the cell, c) cloning the homologue or fragment thereof into an appropriate vector in an orientation relative to a suitable promoter(s) capable of initiating transcription of dsRNA from the DNA homologue or fragment upon binding of an appropriate transcription factor to the promoter(s) and d) introducing the vector into the cell from step a) comprising the transcription factor.

    摘要翻译： 提供了鉴定负责赋予细胞中特定表型的DNA的方法，该方法包括a）在合适的载体中构建相对于能够启动子的启动子的方向的细胞DNA的cDNA或基因组文库 在将合适的转录因子与启动子结合时，启动cDNA或DNA转录为双链（ds）RNA; b）将文库引入至一个或多个包含转录因子的细胞中，c）鉴定和 分离包含文库的细胞的特定表型，并从负责表型的文库鉴定DNA或cDNA片段。 使用这种技术还可以通过以下方式将功能分配给已知的DNA序列：a）鉴定细胞中DNA序列的同系物，b）从细胞中分离相关的DNA同系物或其片段， c）相对于合适的启动子（其能够在合适的转录因子与启动子结合时能够启动dsDNA从DNA同系物或片段的转录）的方向，将其同源物或片段克隆到合适的载体中，并且d ）将载体从包含转录因子的步骤a）引入细胞。

    Method for down-regulating gene expression in fungi
    9.
    发明申请
    Method for down-regulating gene expression in fungi 审中-公开
    在真菌中下调基因表达的方法

    公开(公告)号：US20060247197A1

    公开(公告)日：2006-11-02

    申请号：US11396926

    申请日：2006-04-03

    申请人： Marc Van De Craen ,  Phuay-Yee Goh ,  Marc Logghe ,  Yee-Ling Khu ,  Katherine Mortier ,  Thierry Bogaert

    发明人： Marc Van De Craen ,  Phuay-Yee Goh ,  Marc Logghe ,  Yee-Ling Khu ,  Katherine Mortier ,  Thierry Bogaert

    IPC分类号： A61K48/00 ,  C12N15/74 ,  A01N43/04

    CPC分类号： A01N57/16 ,  A01N63/02 ,  A01N63/04 ,  C07K14/37 ,  C12N15/113 ,  C12N15/8218 ,  C12N15/8282 ,  C12N2310/14

    摘要： The present invention concerns methods for controlling fungus infestation via dsRNA mediated gene silencing, whereby the intact fungus cell(s) are contacted with a double-stranded RNA from outside the fungal cell(s) and whereby the double-stranded RNA is taken up by the intact fungal cell(s). In one particular embodiment, the methods of the invention are used to alleviate plants from fungus pests. Alternatively, the methods are used for treating and/or preventing fungal infestation on a substrate or a subject in need of such treatment and/or prevention. Suitable fungal target genes and fragments thereof, dsRNA constructs, recombinant constructs and compositions are disclosed.

    摘要翻译： 本发明涉及通过dsRNA介导的基因沉默来控制真菌感染的方法，其中完整的真菌细胞与来自真菌细胞外的双链RNA接触，由此双链RNA被 完整的真菌细胞。 在一个具体实施方案中，本发明的方法用于减轻植物从真菌害虫中的减少。 或者，所述方法用于治疗和/或预防对需要这种治疗和/或预防的基质或受试者的真菌侵袭。 公开了合适的真菌靶基因及其片段，dsRNA构建体，重组构建体和组合物。