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    Complementing cell lines
    1.
    发明授权
    Complementing cell lines 有权
    补充细胞系

    公开(公告)号：US06974695B2

    公开(公告)日：2005-12-13

    申请号：US10002750

    申请日：2001-11-15

    申请人： Ronald Vogels ,  Menzo J. E. Havenga ,  Majid Mehtali

    发明人： Ronald Vogels ,  Menzo J. E. Havenga ,  Majid Mehtali

    IPC分类号： C12N15/09 ,  A61K48/00 ,  C12N5/02 ,  C12N5/08 ,  C12N5/10 ,  C12N7/00 ,  C12N7/01 ,  C12N7/02 ,  C12N15/00 ,  C12N15/35 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  C12N15/861

    CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N7/025 ,  C12N15/861 ,  C12N2710/10041 ,  C12N2710/10051 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2830/00 ,  C12N2840/20

    摘要： A packaging cell line capable of complementing recombinant adenoviruses based on serotypes from subgroup B, preferably adenovirus type 35. The cell line is preferably derived from primary, diploid human cells (e.g., primary human retinoblasts, primary human embryonic kidney cells and primary human amniocytes) which are transformed by adenovirus E1 sequences either operatively linked on one DNA molecule or located on two separate DNA molecules, the sequences being operatively linked to regulatory sequences enabling transcription and translation of encoded proteins. Also disclosed is a cell line derived from PER.C6 (ECACC deposit number 96022940), which cell expresses functional Ad35 E1B sequences. The Ad35-E1B sequences are driven by the E1B promoter or a heterologous promoter and terminated by a heterologous poly-adenylation signal. The new cell lines are useful for producing recombinant adenoviruses designed for gene therapy and vaccination. The cell line can also be used for producing human recombinant therapeutic proteins such as human growth factors and human antibodies. In addition, the cell lines are useful for producing human viruses other than adenovirus such as influenza virus, herpes simplex virus, rotavirus, measles virus.

    摘要翻译： 能够基于来自亚组B，优选腺病毒35的血清型补充重组腺病毒的包装细胞系。细胞系优选衍生自原代，二倍体人细胞（例如，原代人成视网膜细胞，原代人胚胎肾细胞和原代人羊膜细胞） 其通过可操作地连接在一个DNA分子上或位于两个分离的DNA分子上的腺病毒E1序列转化，所述序列可操作地连接到能够转录和翻译编码的蛋白质的调控序列。 还公开了衍生自PER.C6（ECACC保藏号96022940）的细胞系，该细胞表达功能性Ad35 E1B序列。 Ad35-E1B序列由E1B启动子或异源启动子驱动，并通过异源多聚腺苷酸化信号终止。 新的细胞系可用于产生用于基因治疗和疫苗接种的重组腺病毒。 细胞系也可用于生产人重组治疗蛋白，如人生长因子和人抗体。 此外，细胞系可用于生产除腺病毒如流感病毒，单纯疱疹病毒，轮状病毒，麻疹病毒之外的人类病毒。

    Complementing cell lines
    2.
    发明授权
    Complementing cell lines 有权
    补充细胞系

    公开(公告)号：US07344883B2

    公开(公告)日：2008-03-18

    申请号：US11165697

    申请日：2005-06-24

    申请人： Ronald Vogels ,  Menzo Jans Emco Havenga ,  Majid Mehtali

    发明人： Ronald Vogels ,  Menzo Jans Emco Havenga ,  Majid Mehtali

    IPC分类号： C12N5/10 ,  C12N5/08 ,  C12N7/00 ,  C12N15/861

    CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N7/025 ,  C12N15/861 ,  C12N2710/10041 ,  C12N2710/10051 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2830/00 ,  C12N2840/20

    摘要： A packaging cell line that complements recombinant adenoviruses based on serotypes from subgroup B, preferably adenovirus type 35. The cell line is preferably derived from primary, diploid human cells that are transformed by adenovirus E1 sequences either operatively linked on one DNA molecule or located on two separate DNA molecules, the sequences being operatively linked to regulatory sequences enabling transcription and translation of encoded proteins. Also disclosed is a cell line derived from PER.C6 that expresses functional Ad35 E1B sequences. The Ad35-E1B sequences are driven by the E1B promoter or a heterologous promoter and terminated by a heterologous poly-adenylation signal. The cell lines are useful for producing recombinant adenoviruses designed for gene therapy and vaccination. The cell lines can also be used for producing human recombinant therapeutic proteins such as human growth factors and human antibodies. Also, the cell lines are useful for producing human viruses other than adenovirus such as influenza virus, herpes simplex virus, rotavirus, and measles virus.

    摘要翻译： 一种包装细胞系，其基于来自亚组B，优选腺病毒35的血清型补充重组腺病毒。细胞系优选衍生自通过腺病毒E1序列转化的原代二倍体人细胞，所述腺病毒E1序列可操作地连接在一个DNA分子上或位于两个 分离的DNA分子，该序列可操作地连接到能够转录和翻译编码蛋白质的调控序列。 还公开了衍生自表达功能性Ad35 E1B序列的PER.C6的细胞系。 Ad35-E1B序列由E1B启动子或异源启动子驱动，并通过异源多聚腺苷酸化信号终止。 细胞系可用于生产设计用于基因治疗和疫苗接种的重组腺病毒。 细胞系也可以用于生产人重组治疗蛋白质，例如人生长因子和人抗体。 此外，细胞系可用于生产除了腺病毒如流感病毒，单纯疱疹病毒，轮状病毒和麻疹病毒之外的人类病毒。

    Complementing cell lines
    3.
    发明授权
    Complementing cell lines 有权
    补充细胞系

    公开(公告)号：US06869794B2

    公开(公告)日：2005-03-22

    申请号：US10164085

    申请日：2002-06-04

    申请人： Ronald Vogels ,  Menzo Havenga ,  Majid Mehtali

    发明人： Ronald Vogels ,  Menzo Havenga ,  Majid Mehtali

    IPC分类号： C12N15/09 ,  A61K48/00 ,  C12N5/02 ,  C12N5/08 ,  C12N5/10 ,  C12N7/00 ,  C12N7/01 ,  C12N7/02 ,  C12N15/00 ,  C12N15/35 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  C12N15/861 ,  C12P1/00 ,  C12P21/06

    CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N7/025 ,  C12N15/861 ,  C12N2710/10041 ,  C12N2710/10051 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2830/00 ,  C12N2840/20

    摘要： A packaging cell line capable of complementing recombinant adenoviruses based on serotypes from subgroup B, preferably adenovirus type 35. The cell line is preferably derived from primary, diploid human cells transformed by adenovirus E1 sequences either operatively linked on one or two DNA molecules, the sequences operatively linked to regulatory sequences enabling transcription and translation of encoded proteins. Also, a cell line derived from PER.C6 that expresses functional Ad35 E1B sequences. The Ad35-E1B sequences are driven by the E1B promoter and terminated by a heterologous poly-adenylation signal. The new cell lines are useful for producing recombinant adenoviruses. The cell lines can be used to produce human recombinant therapeutic proteins such as human antibodies. In addition, the cell lines are useful for producing human viruses other than adenovirus such as influenza, herpes simplex, rotavirus, and measles.

    摘要翻译： 能够基于来自亚组B，优选腺病毒35的血清型补充重组腺病毒的包装细胞系。细胞系优选衍生自由一个或两个DNA分子可操作地连接的腺病毒E1序列转化的原代二倍体人细胞，序列 可操作地连接到能够转录和翻译编码蛋白质的调节序列。 此外，衍生自表达功能性Ad35 E1B序列的PER.C6的细胞系。 Ad35-E1B序列由E1B启动子驱动并通过异源多聚腺苷酸化信号终止。 新的细胞系可用于生产重组腺病毒。 细胞系可用于产生人类重组治疗蛋白，如人抗体。 此外，细胞系可用于产生除腺病毒如流感，单纯疱疹，轮状病毒和麻疹以外的人类病毒。

    Complementing cell lines
    4.
    发明授权
    Complementing cell lines 有权
    补充细胞系

    公开(公告)号：US06492169B1

    公开(公告)日：2002-12-10

    申请号：US09713678

    申请日：2000-11-15

    申请人： Ronald Vogels ,  Menzo Havenga ,  Majid Mehtali

    发明人： Ronald Vogels ,  Menzo Havenga ,  Majid Mehtali

    IPC分类号： C12N502

    CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N7/025 ,  C12N15/861 ,  C12N2710/10041 ,  C12N2710/10051 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2830/00 ,  C12N2840/20

    摘要： A packaging cell line capable of complemention recombinant adenoviruses based on serotypes from subgroup B, preferably adenovirus type 35. The cell line is preferably human embryonic kidney cells and primary human amniocytes) which are transformed by adenovirus E1 sequences either operatively linked on one DNA molecule or located on two separate DNA molecules, the sequences being operatively linked to regulatory sequences enabling transcription and translation of encoded proteins.

    摘要翻译： 一种能够基于来自亚组B（优选腺病毒35型）的血清型互补重组腺病毒的包装细胞系。细胞系优选为人胚胎肾细胞和原代人羊膜细胞），其由在一个DNA分子上有效连接的腺病毒E1序列或 位于两个单独的DNA分子上，该序列可操作地连接到能够转录和翻译编码蛋白的调节序列。

    Complementing cell lines
    5.
    发明申请
    Complementing cell lines 审中-公开

    公开(公告)号：US20080199433A1

    公开(公告)日：2008-08-21

    申请号：US11786409

    申请日：2007-04-11

    申请人： Ronald Vogels ,  Menzo Jans Emco Havenga ,  Majid Mehtali

    发明人： Ronald Vogels ,  Menzo Jans Emco Havenga ,  Majid Mehtali

    IPC分类号： A61K48/00 ,  A61P43/00 ,  C12N7/00 ,  C12N7/04

    CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N7/025 ,  C12N15/861 ,  C12N2710/10041 ,  C12N2710/10051 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2830/00 ,  C12N2840/20

    摘要： A packaging cell line that complements recombinant adenoviruses based on serotypes from subgroup B, preferably adenovirus type 35. The cell line is preferably derived from primary, diploid human cells that are transformed by adenovirus E1 sequences either operatively linked on one DNA molecule or located on two separate DNA molecules, the sequences being operatively linked to regulatory sequences enabling transcription and translation of encoded proteins. Also disclosed is a cell line derived from PER.C6 that expresses functional Ad35 E1B sequences. The Ad35-E1B sequences are driven by the E1B promoter or a heterologous promoter and terminated by a heterologous poly-adenylation signal. The cell lines are useful for producing recombinant adenoviruses designed for gene therapy and vaccination. The cell lines can also be used for producing human recombinant therapeutic proteins such as human growth factors and human antibodies. Also, the cell lines are useful for producing human viruses other than adenovirus such as influenza virus, herpes simplex virus, rotavirus, and measles virus.

    Complementing cell lines
    6.
    发明授权
    Complementing cell lines 有权
    补充细胞系

    公开(公告)号：US07250293B2

    公开(公告)日：2007-07-31

    申请号：US10272041

    申请日：2002-10-15

    申请人： Ronald Vogels ,  Menzo Havenga ,  Majid Mehtali

    发明人： Ronald Vogels ,  Menzo Havenga ,  Majid Mehtali

    IPC分类号： C12N5/10 ,  C12N5/08 ,  C12N15/63 ,  C12N15/861 ,  C12N15/87 ,  A61K48/00

    CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N7/025 ,  C12N15/861 ,  C12N2710/10041 ,  C12N2710/10051 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2830/00 ,  C12N2840/20

    摘要： A packaging cell line capable of complementing recombinant adenoviruses based on serotypes from subgroup B, preferably adenovirus type 35. The cell line is preferably derived from primary diploid human cells transformed by adenovirus E1 sequences either operatively linked on one or two DNA molecules, the sequences operatively linked to regulatory sequences enabling transcription and translation of encoded proteins. Also, a cell line derived from PER.C6 that expresses functional Ad35-E1B sequences. The Ad35-E1B sequences are driven by the E1B promoter and terminated by a heterologous poly-adenylation signal. The new cell lines are useful for producing recombinant adenoviruses. The cell lines can be used to produce human recombinant therapeutic proteins such as human antibodies. In addition, the cell lines are useful for producing human viruses other than adenovirus such as influenza, herpes simplex, rotavirus, and measles.

    摘要翻译： 能够基于来自亚组B，优选腺病毒35的血清型补充重组腺病毒的包装细胞系。细胞系优选衍生自由一个或两个DNA分子可操作地连接的腺病毒E1序列转化的原代二倍体人细胞，所述序列可操作地 与能够转录和翻译编码蛋白质的调控序列相关联。 此外，衍生自PER.C6的表达功能性Ad35-E1B序列的细胞系。 Ad35-E1B序列由E1B启动子驱动并通过异源多聚腺苷酸化信号终止。 新的细胞系可用于生产重组腺病毒。 细胞系可用于产生人类重组治疗蛋白，如人抗体。 此外，细胞系可用于产生除腺病毒如流感，单纯疱疹，轮状病毒和麻疹以外的人类病毒。

    Complementing cell lines
    7.
    发明申请
    Complementing cell lines 有权
    补充细胞系

    公开(公告)号：US20050277194A1

    公开(公告)日：2005-12-15

    申请号：US11165697

    申请日：2005-06-24

    申请人： Ronald Vogels ,  Menzo Havenga ,  Majid Mehtali

    发明人： Ronald Vogels ,  Menzo Havenga ,  Majid Mehtali

    IPC分类号： C12N15/09 ,  A61K48/00 ,  C12N5/02 ,  C12N5/08 ,  C12N5/10 ,  C12N7/00 ,  C12N7/01 ,  C12N7/02 ,  C12N15/00 ,  C12N15/35 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  C12N15/861

    CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N7/025 ,  C12N15/861 ,  C12N2710/10041 ,  C12N2710/10051 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2830/00 ,  C12N2840/20

    摘要： A packaging cell line that complements recombinant adenoviruses based on serotypes from subgroup B, preferably adenovirus type 35. The cell line is preferably derived from primary, diploid human cells that are transformed by adenovirus E1 sequences either operatively linked on one DNA molecule or located on two separate DNA molecules, the sequences being operatively linked to regulatory sequences enabling transcription and translation of encoded proteins. Also disclosed is a cell line derived from PER.C6 that expresses functional Ad35 E1B sequences. The Ad35-E1B sequences are driven by the E1B promoter or a heterologous promoter and terminated by a heterologous poly-adenylation signal. The cell lines are useful for producing recombinant adenoviruses designed for gene therapy and vaccination. The cell lines can also be used for producing human recombinant therapeutic proteins such as human growth factors and human antibodies. Also, the cell lines are useful for producing human viruses other than adenovirus such as influenza virus, herpes simplex virus, rotavirus, and measles virus.

    摘要翻译： 一种包装细胞系，其基于来自亚组B，优选腺病毒35的血清型补充重组腺病毒。细胞系优选衍生自通过腺病毒E1序列转化的原代二倍体人细胞，所述腺病毒E1序列可操作地连接在一个DNA分子上或位于两个 分离的DNA分子，该序列可操作地连接到能够转录和翻译编码蛋白质的调控序列。 还公开了衍生自表达功能性Ad35 E1B序列的PER.C6的细胞系。 Ad35-E1B序列由E1B启动子或异源启动子驱动，并通过异源多聚腺苷酸化信号终止。 细胞系可用于生产设计用于基因治疗和疫苗接种的重组腺病毒。 细胞系也可以用于生产人重组治疗蛋白质，例如人生长因子和人抗体。 此外，细胞系可用于生产除了腺病毒如流感病毒，单纯疱疹病毒，轮状病毒和麻疹病毒之外的人类病毒。

    Chimeric promoters for controlling expression in smooth muscle cells
    9.
    发明授权
    Chimeric promoters for controlling expression in smooth muscle cells 失效
    用于控制平滑肌细胞中表达的嵌合启动子

    公开(公告)号：US07482155B1

    公开(公告)日：2009-01-27

    申请号：US10312963

    申请日：2001-07-04

    申请人： Sébastien Ribault ,  Pascal Neuville ,  Majid Mehtali

    发明人： Sébastien Ribault ,  Pascal Neuville ,  Majid Mehtali

    IPC分类号： C12N15/63 ,  A61K48/00 ,  C07H21/04

    CPC分类号： C12N15/85 ,  A61K48/00 ,  A61K48/0058 ,  C07K14/4716 ,  C12N9/1223 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2799/022 ,  C12N2830/008 ,  C12N2830/15 ,  C12N2830/85

    摘要： The present invention concerns a chimeric construct comprising a SMC-specific promoter operably linked to a muscle-specific enhancer. It also provides an expression cassette comprising such a chimeric construct to control expression of a therapeutic gene. Finally, the invention relates to a recombinant vector, a viral particle, an eukaryotic host cell, a composition comprising said expression cassette and their use for specific expression in SMCs and for therapeutic or prophylactic purposes, a method for the treatment of a human or animal organism as well as a transgenic non-human animal comprising integrated into its genome the chimeric construct, the expression cassette or the vector of the present invention.

    摘要翻译： 本发明涉及包含与肌肉特异性增强子可操作地连接的SMC特异性启动子的嵌合构建体。 它还提供了包含这种嵌合构建体以控制治疗基因表达的表达盒。 最后，本发明涉及重组载体，病毒颗粒，真核宿主细胞，包含所述表达盒的组合物及其在SMC中特异性表达和用于治疗或预防目的的用途，用于治疗人或动物的方法 生物体以及包含在其基因组中整合到本发明的嵌合构建体，表达盒或载体中的转基因非人动物。