利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

9 条记录 (耗时 0.023 秒)

    Process for producing 7-dimethylamino-6-demethyl-6-deoxytetracycline
    2.
    发明授权
    Process for producing 7-dimethylamino-6-demethyl-6-deoxytetracycline 失效
    7-二甲基氨基-6-脱甲基-6-脱氧四环素的制备方法

    公开(公告)号：US4918208A

    公开(公告)日：1990-04-17

    申请号：US222886

    申请日：1988-07-22

    申请人： Ryoichi Hasegawa ,  Hiroaki Ohno ,  Kazuaki Sano ,  Yoshinori Saito

    发明人： Ryoichi Hasegawa ,  Hiroaki Ohno ,  Kazuaki Sano ,  Yoshinori Saito

    IPC分类号： C07C231/22 ,  C07C237/26

    CPC分类号： C07C231/22 ,  C07C237/26 ,  C07C2103/46

    摘要： A process for producing 7-dimethylamino-6-demethyl-6-deoxytetracycline (minocycline) which comprises treating the reaction liquid containing minocycline and formaldehyde with hydroxylamine or urea, the reaction liquid being obtained after reductive methylation of 7-amino-6-demethyl-6-deoxytetracycline by using aldehyde, then subjecting the reaction liquid to an adsorption treatment with a nonionic adsorption resin (adsorbent), and subsequently eluting the intended product adsorbed to the adsorbent.

    摘要翻译： 一种7-二甲基氨基-6-脱氧四环素（二甲胺四环素）的制备方法，其包括用羟胺或尿素处理含有二甲胺四环素和甲醛的反应液体，所述反应液是在还原甲基化7-氨基-6-去甲基 - 通过使用醛，然后用非离子吸附树脂（吸附剂）对反应液进行吸附处理，随后洗脱吸附在吸附剂上的预期产物。

    Method for measurement of tissue factor in high sensitivity and measurement kit therefor
    5.
    发明授权
    Method for measurement of tissue factor in high sensitivity and measurement kit therefor 失效
    高灵敏度组织因子测量方法及其测量套件

    公开(公告)号：US5403716A

    公开(公告)日：1995-04-04

    申请号：US924030

    申请日：1992-09-03

    申请人： Kimihiko Matsuzawa ,  Ryoichi Hasegawa

    发明人： Kimihiko Matsuzawa ,  Ryoichi Hasegawa

    IPC分类号： C07K16/36 ,  G01N33/86 ,  G01N33/544 ,  G01N33/535 ,  G01N33/551 ,  G01N33/577

    CPC分类号： C07K16/36 ,  G01N33/86 ,  G01N2333/7454 ,  Y10S435/81 ,  Y10S436/826

    摘要： The present invention provides an improved buffer solution for conducting an immunological method for measuring apoprotein of human tissue factor, and a kit therefor. More specifically, in a method for immunologically measuring the presence or concentration of apoprotein of human tissue factor contained in a sample, which method includes the steps of (1) forming in a buffer solution a ternary complex of (a) a first monoclonal antibody which is capable of specifically binding to the apoprotein and is immobilized on a solid carrier insoluble in the buffer solution, (b) the apoprotein and (c) a labeled second monoclonal antibody which is capable of specifically binding to the apoprotein at a different site from the binding site of the first monoclonal antibody, and (2) measuring the amount of labeled second monoclonal antibody in the ternary complex to determine the presence or concentration of apoprotein in the sample, the improvement of this invention comprising employing a buffer solution(i) containing in a concentration of 2 to 15 weight % at least one nonionic surfactant having an HLB value within a range of 12 to 30, and(ii) containing a protein having a molecular weight of about 16,000 to about 50,000 and an isoelectric point of 1.0 to 5.0.

    摘要翻译： PCT No.PCT / JP92 / 00005 Sec。 371日期：1992年9月3日 102（e）1992年9月3日PCT PCT 1992年1月8日PCT公布。 出版物WO92 / 日本1992年7月23日。本发明提供了一种改进的缓冲溶液，用于进行用于测量人组织因子的脱蛋白的免疫学方法及其试剂盒。 更具体地，在用于免疫测定样品中包含的人组织因子的脱辅基蛋白的存在或浓度的方法中，所述方法包括以下步骤：（1）在缓冲溶液中形成（a）第一单克隆抗体的三元配合物，所述第一单克隆抗体 能够特异性结合载脂蛋白并固定在不溶于缓冲溶液的固体载体上，（b）载脂蛋白和（c）标记的第二单克隆抗体，其能够与不同部位的脱辅基蛋白特异性结合 第一单克隆抗体的结合位点，和（2）测量三元复合物中标记的第二单克隆抗体的量以确定样品中脱辅基蛋白的存在或浓度，本发明的改进包括使用缓冲溶液（i） 浓度为2至15重量％的至少一种HLB值在12至30范围内的非离子表面活性剂，和（ii）含有蛋白质 n的分子量为约16,000至约50,000，等电点为1.0至5.0。

    Process for producing optically active phenylalanine
    6.
    发明授权
    Process for producing optically active phenylalanine 失效
    光学活性苯丙氨酸的制备方法

    公开(公告)号：US4582928A

    公开(公告)日：1986-04-15

    申请号：US716400

    申请日：1985-03-27

    申请人： Shigeru Aoki ,  Mamoru Katagiri ,  Ryoichi Hasegawa ,  Akikazu Mitsunobu ,  Yasuhisa Tashiro

    发明人： Shigeru Aoki ,  Mamoru Katagiri ,  Ryoichi Hasegawa ,  Akikazu Mitsunobu ,  Yasuhisa Tashiro

    IPC分类号： C07B57/00 ,  C07B31/00 ,  C07C67/00 ,  C07C227/34 ,  C07C229/36 ,  C07C101/08

    CPC分类号： C07C227/34

    摘要： This invention relates to a process for producing optically active phenylalanine, characterized by optically resolving DL-phenylalanine.optically active mandelic acid complexes in an aqueous solvent in the presence of an acidic compound having a pKa value of 0.90 to 2.10 and isolating optically active phenylalanine from the obtained optically active phenylalanine.optically active mandelic acid complex.According to the process of the present invention, it is possible to decrease the amount of a mother liquor of resolution to 1/5 to 1/10 of that when no acidic compound is used and to obtain optically active phenylalanine having an optical purity of as high as 96.5% or above without a step of optical purification when optically active phenylalanine is isolated from the obtained optically active phenylalanine.optically active mandelic acid complex.

    摘要翻译： 本发明涉及一种光学活性苯丙氨酸的制备方法，其特征在于在pKa值为0.90至2.10的酸性化合物存在下，在水性溶剂中光学拆分DL-苯丙氨酸光学活性扁桃酸配合物，并将光学活性苯丙氨酸从 所得光学活性苯丙氨酸光学活性扁桃酸复合物。 根据本发明的方法，可以将分辨率降低至不使用酸性化合物时的1/5至1/10的量，并获得具有光学纯度的光学活性苯丙氨酸 光学活性苯丙氨酸光学活性苯丙氨酸复合物中分离出光学活性苯丙氨酸时，不需要光学纯化步骤，高达96.5％以上。

    Human tissue plasminogen activator-human plasminogen activator inhibitor complex immunoassay and kit therefor
    8.
    发明授权
    Human tissue plasminogen activator-human plasminogen activator inhibitor complex immunoassay and kit therefor 失效
    人体组织纤溶酶原激活物 - 人纤溶酶原激活物抑制剂复合物免疫测定及其试剂盒

    公开(公告)号：US5352583A

    公开(公告)日：1994-10-04

    申请号：US687867

    申请日：1991-06-03

    申请人： Yoichi Sakata ,  Kazuhiko Itoh ,  Shuichiro Hino ,  Ryoichi Hasegawa ,  Naomi Okamoto ,  Atsushi Noro ,  Toshinobu Murakami

    发明人： Yoichi Sakata ,  Kazuhiko Itoh ,  Shuichiro Hino ,  Ryoichi Hasegawa ,  Naomi Okamoto ,  Atsushi Noro ,  Toshinobu Murakami

    IPC分类号： G01N33/543 ,  G01N33/86 ,  G01N33/53

    CPC分类号： G01N33/54393 ,  G01N33/54306 ,  G01N33/86 ,  G01N2333/8132 ,  G01N2333/9726 ,  Y10S435/962

    摘要： A kit for the immunological assay of the human tissue plasminogen activator-human plasminogen activator inhibitor complex in a human specimen, which kit comprises(i) a monoclonal antibody (first antibody) against a human plasminogen activator inhibitor linked onto an insoluble solid carrier having a specular surface,(ii) a polyclonal antibody (second antibody) against a human tissue plasminogen activator labelled by an enzyme,(iii) a substrate and a reaction-discontinuing agent for the assay of the enzyme activity,(iv) a diluent, and(v) a detergent containing a nonionic surfactant having an HLB (Hydrophile Lipophile Balance) value of at least 16,and a method for the immunological assay of the above complex and a method for the immunological assay of the active human plasminogen activator inhibitor, respectively in a human specimen using this kit.

    摘要翻译： PCT No.PCT / JP90 / 01269 Sec。 371日期1991年6月3日 102（e）日期1991年6月3日PCT 1990年10月2日PCT PCT。 公开号WO91 / 05257 日期：1991年4月18日。用于人体标本中人组织纤溶酶原激活物 - 人纤溶酶原激活物抑制剂复合物的免疫测定试剂盒，该试剂盒包含（i）针对人类纤溶酶原激活物抑制剂的单克隆抗体（第一抗体） 到具有镜面的不溶性固体载体上，（ii）针对由酶标记的人组织纤溶酶原激活物的多克隆抗体（第二抗体），（iii）用于测定酶活性的底物和反应中断剂， （iv）稀释剂，和（v）含有HLB（亲水亲油平衡）值至少为16的非离子表面活性剂的洗涤剂，以及上述复合物的免疫学测定方法和 活性人纤溶酶原激活物抑制剂，分别在使用该试剂盒的人体标本中。

    Process for producing substituted or unsubstituted naphthalic acids and acid anhydrides thereof
    9.
    发明授权
    Process for producing substituted or unsubstituted naphthalic acids and acid anhydrides thereof 失效
    制备取代或未取代的萘二甲酸及其酸酐的方法

    公开(公告)号：US4212813A

    公开(公告)日：1980-07-15

    申请号：US950407

    申请日：1978-10-11

    申请人： Ryoichi Hasegawa

    发明人： Ryoichi Hasegawa

    IPC分类号： C07C51/31 ,  C07C205/57 ,  C07B3/00

    CPC分类号： C07C205/57 ,  C07C201/12 ,  C07C51/313 ,  C07C51/56 ,  C07D307/92

    摘要： Process for producing substituted or unsubstituted naphthalic acids and acid anhydrides thereof by oxidation of corresponding acenaphthenes whose naphthalene nucleus may have one or two substituents from the group consisting of halogens, sulfonic acid groups, sulfonate groups, and nitro. The oxidation is performed at 30.degree.-150.degree. C. using molecular oxygen in the presence of (1) at least one heavy metal compound, such as cobalt acetate or manganese acetate, in an amount of 0.005-0.3 mole per mole of said acenaphthenes or a mixture of heavy metal compounds in that amount and a bromine compound in the range of 5.times.10.sup.-5 to 2.times.10.sup.-1 mole/liter of reaction solution as a catalyst and (2) at least one accelerator selected from the group consisting of lower fatty acid anhydrides, ketones and aldehydes, whereby side reactions such as condensation can be suppressed to a satisfactory extent, thereby leading to an improvement in yield of final product. A subsequent treatment of the oxidation product obtained by this process with peroxides, hypohalogenates, or molecular oxygen results in a higher yield.

    摘要翻译： 通过氧化相应的苊的方法制备取代或未取代的萘二甲酸及其酸酐，其萘核可以具有一个或两个来自卤素，磺酸基，磺酸酯基和硝基的取代基。 在（1）至少一种重金属化合物如乙酸钴或乙酸锰存在下，使用分子氧在30-150℃进行氧化，其量为每摩尔所述苊的0.005-0.3摩尔 或这种量的重金属化合物的混合物和作为催化剂的反应溶液的5×10 -5至2×10 -1摩尔/升范围内的溴化合物和（2）至少一种选自低脂肪 酸酐，酮和醛，由此可以将缩合的副反应抑制到令人满意的程度，从而导致最终产物的收率提高。 用过氧化物，次卤酸盐或分子氧随后处理通过该方法获得的氧化产物导致更高的产率。