公开(公告)号：US12241863B2

公开(公告)日：2025-03-04

申请号：US17158239

申请日：2021-01-26

Applicant: SHIMADZU CORPORATION

Inventor： Hidesato Kumagai ,  Akihiro Arai ,  Akira Harada

IPC: G01N27/447 ,  G01N27/453

Abstract: A controller sets a schedule in which an order of a plurality of electrophoresis processes is defined. The controller controls a storage device to store first information representing a result of an electrophoresis process that has ended among the plurality of electrophoresis processes and second information about setting for the plurality of electrophoresis processes. The controller receives a stop signal from an information processing apparatus while the plurality of electrophoresis processes defined in the schedule are being performed. When the controller receives the stop signal, the controller controls an electrophoresis mechanism to perform until the end, an electrophoresis process that is being performed among the plurality of electrophoresis processes. When the controller receives the stop signal, the controller maintains storage of the first information stored in the storage device and the second information on an electrophoresis process that has not yet been performed among the plurality of electrophoresis processes.

公开(公告)号：US11085897B2

公开(公告)日：2021-08-10

申请号：US16130011

申请日：2018-09-13

Applicant: SHIMADZU CORPORATION

Inventor： Akihiro Arai

IPC: G01N27/447 ,  B08B9/032 ,  B08B3/04

Abstract: It is an object to repeatedly use a microchip without complicating the structure of the microchip, as well as not impairing cost and operability. A seal-attached member 26 is disposed facing a microchip 5 held by a chip holding unit 7, and has through holes 64-3 and 64-4 provided at positions corresponding to reservoirs 53-3 and 53-4, respectively, and elastic members 67 that are pressed against the microchip 5 so as to maintain airtightness between the through holes 64-3 and 64-4 and the corresponding reservoirs 53-3 and 53-4. When the inside of a flow path 55 of the microchip 5 is cleaned, a dispensing probe 8 is inserted into the through hole 64-4 while airtightness between the through hole 64-4 and the dispensing probe 8 is maintained. Then, a suction nozzle 22-3 is inserted into the through hole 64-3. In this state, the dispensing probe 8 discharges a cleaning liquid to cause the cleaning liquid to flow from the reservoir 53-4 under the pressurizing port through hole 64-4 into the flow path 55, and the suction nozzle 22-3 sucks the cleaning liquid discharged from the flow path 55 to the reservoir 53-3.

公开(公告)号：US10018976B2

公开(公告)日：2018-07-10

申请号：US14609762

申请日：2015-01-30

Applicant: SHIMADZU CORPORATION

Inventor： Akihiro Arai

IPC: G01N27/447 ,  G05B15/02 ,  C12N15/10

CPC classification number: G05B15/02 ,  C12N15/101 ,  G01N27/44747

Abstract: Resolution maps for size segments to which the size range of the measurement sample extends are retrieved. A high resolution region is extracted from the retrieved size segment resolution maps. After extracting the high resolution regions, the resolution maps are overlaid, the region where the high resolution regions overlap is taken as a high resolution overlap region, and the composition of a point within that high resolution overlap region is determined as the composition of a separation medium appropriate for separation of the measurement sample. The separation media A, B and C are mixed so as to achieve the determined composition, thereby preparing a mixed separation medium to be used for separation of the measurement sample.

公开(公告)号：US09303239B2

公开(公告)日：2016-04-05

申请号：US13778810

申请日：2013-02-27

Applicant: SHIMADZU CORPORATION

Inventor： Akihiro Arai

IPC: B08B3/08 ,  B08B3/04 ,  B08B9/00 ,  C11D7/50 ,  C11D7/26 ,  C11D7/28 ,  C11D7/34 ,  C11D11/00 ,  B01L3/00 ,  G01N27/447 ,  G01N30/60

CPC classification number: C11D7/5022 ,  B01L3/502753 ,  B08B3/04 ,  B08B3/08 ,  B08B9/00 ,  C11D7/265 ,  C11D7/28 ,  C11D7/34 ,  C11D7/5004 ,  C11D7/5013 ,  C11D11/0023 ,  G01N27/44791 ,  G01N30/6095

Abstract: The present invention provides a cleaning method by which the performance of a microchip can be recovered and a cleaning liquid. A method for cleaning a microfluidic device comprising: cleaning a channel that is formed in the microfluidic device, has a surface having a polymer coating, and has been brought into contact with a sample containing nucleic acid and/or protein, by bringing the channel into contact with a cleaning liquid comprised only of an organic solvent having solubility in at least the same volume of water at 25° C., or a cleaning liquid containing 50 vol % or more of the organic solvent in a buffer solution. The method wherein the buffer solution has a pH of 8 to 10. The method wherein the buffer solution further contains 3 to 8M of a protein denaturant. The method wherein the buffer solution has a pH of 2 to 4.

Abstract translation: 本发明提供一种能够回收微芯片性能的清洗方法和清洗液。 一种用于清洁微流体装置的方法，包括：清洁在微流体装置中形成的通道，具有聚合物涂层的表面，并且已经与含有核酸和/或蛋白质的样品接触，通过使通道进入 与仅在25℃下至少相同体积的水中溶解的有机溶剂的清洗液或在缓冲溶液中含有50体积％以上有机溶剂的清洗液接触。 其中缓冲溶液的pH为8〜10的方法，其中缓冲液还含有3〜8M的蛋白质变性剂。 缓冲溶液的pH为2〜4的方法。

公开(公告)号：US10359394B2

公开(公告)日：2019-07-23

申请号：US15632798

申请日：2017-06-26

Applicant: SHIMADZU CORPORATION

Inventor： Akihiro Arai ,  Taigo Nishida

IPC: G01N27/453 ,  G01N27/447 ,  G01N21/64 ,  G01N21/03

Abstract: In a capillary electrophoresis device, detection window sections of a plurality of capillaries are arranged, and a capillary cassette is mounted and used, the capillary cassette having an excitation-side positioning part and a fluorescence-side positioning part so as to hold the detection window section array therebetween. The capillary electrophoresis device includes an excitation optical system unit and a fluorescence-receiving unit having positioning parts positioned to the capillary cassette. The excitation optical system unit includes a rod-like lens array configured to irradiate an excitation light to each detection window section. The fluorescence-receiving unit includes a rod-like lens array configured to receive a fluorescence from each detection window section, and an optical fiber for each rod-like lens configured to guide fluorescence from the rod-like lens to a detector side.

公开(公告)号：US12285756B2

公开(公告)日：2025-04-29

申请号：US17602621

申请日：2020-01-27

Applicant: SHIMADZU CORPORATION

Inventor： Akihiro Arai

IPC: B01L3/00 ,  B01F33/302 ,  B01F33/3033 ,  B01L7/00 ,  C12Q1/6848 ,  C12Q1/686 ,  G01N21/29 ,  G01N35/10

Abstract: A microfluidic system includes a chip holder that holds a microchip including a plurality of reservoirs, a chip cover, a sealing member; a dispensing probe, a suction mechanism, a first storage where a first cleaning solution is stored, a second storage where a second cleaning solution is stored, a pump, a channel switch, and a controller. The channel switch is configured to switch between a channel through which the first cleaning solution is suctioned from the first storage and a channel through which the second cleaning solution is suctioned from the second storage. The controller controls operations of the channel switch and the pump.

公开(公告)号：US20230010554A1

公开(公告)日：2023-01-12

申请号：US17857294

申请日：2022-07-05

Applicant: SHIMADZU CORPORATION

Inventor： Akira HARADA ,  Akihiro Arai ,  Hidesato Kumagai

IPC: G01N27/447

Abstract: An electrophoresis apparatus includes a dilution unit, an electrophoresis unit, and a control device. The dilution unit dilutes a sample with dilution water. The electrophoresis unit analyzes the sample diluted by the dilution unit by electrophoresing the sample. The control device controls the dilution unit and the electrophoresis unit.

公开(公告)号：US10088449B2

公开(公告)日：2018-10-02

申请号：US14974647

申请日：2015-12-18

Applicant: SHIMADZU CORPORATION

Inventor： Akihiro Arai ,  Toru Kaji

IPC: G01N27/447

Abstract: A capillary electrophoresis device including a capillary tube, a suction pump for taking liquid, an intake tube whose end is formed vertically downward, a connection block in which there is an intake flow path that holds the end of the capillary tube and connecting the suction pump to the intake tube, a sample storage unit which contains a sample and has an upward opening into which the tip of the intake tube may be inserted, an intake tube access mechanism to insert the tip of the intake tube into the sample storage unit, and a voltage application mechanism that applies an electric potential difference across the capillary tube.

公开(公告)号：US20140238444A1

公开(公告)日：2014-08-28

申请号：US13778810

申请日：2013-02-27

Applicant: SHIMADZU CORPORATION

Inventor： Akihiro Arai

IPC: C11D7/50

CPC classification number: C11D7/5022 ,  B01L3/502753 ,  B08B3/04 ,  B08B3/08 ,  B08B9/00 ,  C11D7/265 ,  C11D7/28 ,  C11D7/34 ,  C11D7/5004 ,  C11D7/5013 ,  C11D11/0023 ,  G01N27/44791 ,  G01N30/6095

Abstract: The present invention provides a cleaning method by which the performance of a microchip can be recovered and a cleaning liquid. A method for cleaning a microfluidic device comprising: cleaning a channel that is formed in the microfluidic device, has a surface having a polymer coating, and has been brought into contact with a sample containing nucleic acid and/or protein, by bringing the channel into contact with a cleaning liquid comprised only of an organic solvent having solubility in at least the same volume of water at 25° C., or a cleaning liquid containing 50 vol % or more of the organic solvent in a buffer solution. The method wherein the buffer solution has a pH of 8 to 10. The method wherein the buffer solution further contains 3 to 8M of a protein denaturant. The method wherein the buffer solution has a pH of 2 to 4.

Abstract translation: 本发明提供一种能够回收微芯片性能的清洗方法和清洗液。 一种用于清洁微流体装置的方法，包括：清洁在微流体装置中形成的通道，具有聚合物涂层的表面，并且已经与含有核酸和/或蛋白质的样品接触，通过使通道进入 与仅在25℃下至少相同体积的水中溶解的有机溶剂的清洗液或在缓冲溶液中含有50体积％以上有机溶剂的清洗液接触。 其中缓冲溶液的pH为8〜10的方法，其中缓冲液还含有3〜8M的蛋白质变性剂。 缓冲溶液的pH为2〜4的方法。