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公开(公告)号：US09850509B2
公开(公告)日：2017-12-26
申请号：US14905326
申请日：2013-12-20
发明人： Chang Kuk Kim , Yeon Hwa La , Joong Min Park , Hong Seung Yeon , Cher Hee Park
CPC分类号： C12P7/6427 , C11B1/02 , C11B3/005 , C12M21/02 , C12M47/06 , C12P7/6472
摘要： Provided are an oleaginous microorganism disruption process using a supersonic disperser and a method for producing bio-oil using the same. The method for producing bio-oil according to the present invention induces a cell disruption of oleaginous microorganisms without a separate drying process, thereby providing a method for continuously producing bio-oil in an economical and simple manner. In addition, the method of the present invention induces a cell disruption of oleaginous microorganisms without a heating process, thereby producing bio-oil without a change in physical properties due to the heat.
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2.发明申请Oleaginous Microorganism Disruption Process Using Supersonic Disperser and Method for Producing Bio-Oil Using Same 有权使用超音速分散器的油性微生物破坏过程及使用其生产生物油的方法公开(公告)号：US20160160246A1
公开(公告)日：2016-06-09
申请号：US14905326
申请日：2013-12-20
发明人： Chang Kuk Kim , Yeon Hwa La , Joong Min Park , Hong Seung Yeon , Cher Hee Park
IPC分类号： C12P7/64
CPC分类号： C12P7/6427 , C11B1/02 , C11B3/005 , C12M21/02 , C12M47/06 , C12P7/6472
摘要： Provided are an oleaginous microorganism disruption process using a supersonic disperser and a method for producing bio-oil using the same. The method for producing bio-oil according to the present invention induces a cell disruption of oleaginous microorganisms without a separate drying process, thereby providing a method for continuously producing bio-oil in an economical and simple manner. In addition, the method of the present invention induces a cell disruption of oleaginous microorganisms without a heating process, thereby producing bio-oil without a change in physical properties due to the heat.
摘要翻译： 提供使用超音速分散器的油性微生物破坏方法和使用其的生物油的生产方法。 根据本发明的生物油的制造方法在没有单独的干燥过程的情况下引起含油微生物的细胞破裂，从而提供了以经济和简单的方式连续生产生物油的方法。 此外，本发明的方法在不加热的情况下引起含油微生物的细胞破裂，由此产生生物油，而不会由于热而物理性质的变化。
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3.发明授权公开(公告)号：US09745606B2
公开(公告)日：2017-08-29
申请号：US14813689
申请日：2015-07-30
发明人： Joong Min Park , Chang Kuk Kim , Yeon Hwa La , Young Bin Seo
IPC分类号： C12P7/64 , C12N1/12 , C11B1/02 , C11B1/04 , C11B1/10 , C11B3/00 , C11B3/10 , C11B3/14 , C11B7/00 , C12R1/89
CPC分类号： C12P7/6409 , C11B1/025 , C11B1/04 , C11B1/10 , C11B3/001 , C11B3/008 , C11B3/10 , C11B3/14 , C11B7/0075 , C12N1/12 , C12R1/89
摘要： Provided herein are microalgae of a Thraustochytrid and a method for preparing bio-oil using the same, and more particularly, Aurantiochytrium sp. LA3 (KCTC12685BP) having bio-oil producibility, and a method of preparing bio-oil, particularly bio-oil having a content of omega-3 unsaturated fatty acids of 30% by weight or more based on total fatty acids, characterized by culturing the microalgae. The microalgae Aurantiochytrium sp. LA3 (KCTC12685BP) described herein has a rapid sugar consumption rate when being cultured using glucose as a carbon source, has a high oil content, allows cells to be cultured at a high concentration, and allows oil to be obtained in high productivity and a high yield, and thus, may produce bio-oil more economically and environmentally friendly.
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公开(公告)号：US10704064B2
公开(公告)日：2020-07-07
申请号：US15506981
申请日：2015-08-28
发明人： Joong Min Park , Jae Yeon Park , Woo Chan Park , Sang Min Lee , Young Bin Seo , Merja Oja , Outi Koivistoinen , Andrew Conley , Marja Ilmen , Laura Ruohonen , Paula Jouhten
摘要： Provided are a recombinant yeast producing 3-hydroxypropionic acid (3-HP) and a method for producing 3-HP using the same, more particularly, a recombinant yeast producing 3-HP, comprising an exogenous AADH gene; an endogenous or exogenous ACC gene; an exogenous MCR gene; and an exogenous HPDH gene, and producing 3-HP through [Pyruvate Acetaldehyde→Acetyl-CoA Malonyl-CoA Malonate semialdehyde 3-HP] biosynthesis pathway, and a method for producing 3-HP using the same.
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5.发明申请公开(公告)号：US20170240932A1
公开(公告)日：2017-08-24
申请号：US15506981
申请日：2015-08-28
发明人： Joong Min Park , Jae Yeon Park , Woo Chan Park , Sang Min Lee , Young Bin Seo , Merja Oja , Outi Koivistoinen , Andrew Conley , Marja Ilmen , Laura Ruohonen , Paula Jouhten
CPC分类号： C12P7/42 , C12N1/16 , C12N1/18 , C12N9/0006 , C12N9/0008 , C12N9/93 , C12N15/52 , C12Y101/01059 , C12Y102/0101 , C12Y102/01075 , C12Y604/01002
摘要： Provided are a recombinant yeast producing 3-hydroxypropionic acid (3-HP) and a method for producing 3-HP using the same, more particularly, a recombinant yeast producing 3-HP, comprising an exogenous AADH gene; an endogenous or exogenous ACC gene; an exogenous MCR gene; and an exogenous HPDH gene, and producing 3-HP through [Pyruvate Acetaldehyde→Acetyl-CoA Malonyl-CoA Malonate semialdehyde 3-HP] biosynthesis pathway, and a method for producing 3-HP using the same.
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6.发明申请公开(公告)号：US20170327851A1
公开(公告)日：2017-11-16
申请号：US15660181
申请日：2017-07-26
发明人： Joong Min Park , Chang Kuk Kim , Yeon Hwa La , Young Bin Seo
IPC分类号： C12P7/64 , C12N1/12 , C11B7/00 , C11B3/14 , C11B3/10 , C11B3/00 , C11B1/10 , C11B1/04 , C12R1/89 , C11B1/02
CPC分类号： C12P7/6409 , C11B1/025 , C11B1/04 , C11B1/10 , C11B3/001 , C11B3/008 , C11B3/10 , C11B3/14 , C11B7/0075 , C12N1/12 , C12R1/89
摘要： Provided herein are microalgae of a Thraustochytrid and a method for preparing bio-oil using the same, and more particularly, Aurantiochytrium sp. LA3 (KCTC12685BP) having bio-oil producibility, and a method of preparing bio-oil, particularly bio-oil having a content of omega-3 unsaturated fatty acids of 30% by weight or more based on total fatty acids, characterized by culturing the microalgae. The microalgae Aurantiochytrium sp. LA3 (KCTC12685BP) described herein has a rapid sugar consumption rate when being cultured using glucose as a carbon source, has a high oil content, allows cells to be cultured at a high concentration, and allows oil to be obtained in high productivity and a high yield, and thus, may produce bio-oil more economically and environmentally friendly.
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公开(公告)号：US10941351B2
公开(公告)日：2021-03-09
申请号：US15796941
申请日：2017-10-30
发明人： Sung Ho Lee , Dong Hyun Kim , Jae Yang Song , Joong Min Park , Sung Wook Jun
摘要： Provided are a layer-separation method of a spent caustic solution, and a recycling method of an additive, and more particularly, a layer-separation method of a spent caustic solution including: injecting an additive and an acidic compound to the spent caustic solution occurring from a refinery process to break down a red oil emulsion and to perform layer-separation into an upper layer fraction and a lower layer fraction, wherein the additive is an aliphatic hydrocarbon compound having a water solubility of 0.1 to 10 g/L at 20° C.
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公开(公告)号：US20180119025A1
公开(公告)日：2018-05-03
申请号：US15796941
申请日：2017-10-30
发明人： Sung Ho Lee , Dong Hyun Kim , Jae Yang Song , Joong Min Park , Sung Wook Jun
CPC分类号： C10G19/08 , B01D11/04 , B01D11/0488 , B01D11/0492 , B01D17/047 , C07C31/125 , C07C69/14 , C07C69/24 , C10G33/04 , C10G2300/202 , C10G2300/4081
摘要： Provided are a layer-separation method of a spent caustic solution, and a recycling method of an additive, and more particularly, a layer-separation method of a spent caustic solution including: injecting an additive and an acidic compound to the spent caustic solution occurring from a refinery process to break down a red oil emulsion and to perform layer-separation into an upper layer fraction and a lower layer fraction, wherein the additive is an aliphatic hydrocarbon compound having a water solubility of 0.1 to 10 g/L at 20° C.
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9.发明申请Microalgae Aurantiochytrium sp. LA3 (KCTC12685BP) and Method for Preparing Bio-Oil Using the Same 有权微藻Aurantiochytrium sp。 LA3（KCTC12685BP）及使用其制备生物油的方法公开(公告)号：US20160102328A1
公开(公告)日：2016-04-14
申请号：US14813689
申请日：2015-07-30
发明人： Joong Min Park , Chang Kuk Kim , Yeon Hwa La , Young Bin Seo
CPC分类号： C12P7/6409 , C11B1/025 , C11B1/04 , C11B1/10 , C11B3/001 , C11B3/008 , C11B3/10 , C11B3/14 , C11B7/0075 , C12N1/12 , C12R1/89
摘要： Provided herein are microalgae of a Thraustochytrid and a method for preparing bio-oil using the same, and more particularly, Aurantiochytrium sp. LA3 (KCTC12685BP) having bio-oil producibility, and a method of preparing bio-oil, particularly bio-oil having a content of omega-3 unsaturated fatty acids of 30% by weight or more based on total fatty acids, characterized by culturing the microalgae. The microalgae Aurantiochytrium sp. LA3 (KCTC12685BP) described herein has a rapid sugar consumption rate when being cultured using glucose as a carbon source, has a high oil content, allows cells to be cultured at a high concentration, and allows oil to be obtained in high productivity and a high yield, and thus, may produce bio-oil more economically and environmentally friendly.
摘要翻译： 本文提供了Thraustochytrid的微藻和使用其制备生物油的方法，更具体地，Aurantiochytrium sp。 具有生物油生产性的LA3（KCTC12685BP），以及基于总脂肪酸制备生物油，特别是具有30重量％以上的ω-3不饱和脂肪酸含量的生物油的方法，其特征在于， 微藻类 微藻Aurantiochytrium sp。 本文所述的LA3（KCTC12685BP）在使用葡萄糖作为碳源进行培养时具有快速的糖消耗率，具有高油含量，允许细胞以高浓度培养，并且以高生产率和高的产率获得油 产量，从而可以在经济上和环境友好的情况下生产生物油。
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10.发明申请METHOD FOR PREPARING ORGANIC ACID BY FED-BATCH-FEEDING CARBON SOURCE SUBSTRATE AND BASE 审中-公开通过FED填充碳源基材和基料制备有机酸的方法公开(公告)号：US20150140615A1
公开(公告)日：2015-05-21
申请号：US14402980
申请日：2013-05-21
发明人： Jae Yeon Park , Sin Young Kang , Woo Chan Park , Min Su Koo , In Ho Cho , Joong Min Park , Seung Yeop Lee , Dong Hyun Kim
IPC分类号： C12P7/52
摘要： The present invention provides a fed-batch culture method comprising a step of fed-batch-feeding a carbon source base and a base in such a manner that the pH level can be maintained at a level suitable for the growth of microorganisms for fermentation of a carbon source. The present invention also provides a method for preparing organic acids using the fed-batch culture method. The present invention fed-batch-feeds a neutralizing agent such as ammonium bicarbonate, ammonium carbonate or alkali metal-containing weak base, and a carbon source substrate in preparing organic acids by microorganism fermentation. Thus, a pH level suitable for the survival of microorganisms for carbon source fermentation can be maintained, and the speed of injecting the carbon source base which is the source material can be appropriately adjusted. The present invention may improve productivity, yield rate and concentration of organic acids and may automatically inject a base and a carbon source substrate according to a variation in the pH level, thus improving reliability and convenience of a fermentation process operation.
摘要翻译： 本发明提供了一种补料分批培养方法，其包括以下步骤的补料分批进料步骤：以使得pH水平可以保持在适于微生物生长的水平的方式，以发酵 碳源。 本发明还提供了使用补料分批培养方法制备有机酸的方法。 本发明通过微生物发酵补料分批供料中和剂如碳酸氢铵，碳酸铵或含碱金属的弱碱和碳源底物制备有机酸。 因此，可以维持适于碳源发酵的微生物的存活的pH值，可以适当调整作为源材料的碳源基材的注入速度。 本发明可以提高生产率，产率和有机酸的浓度，并且可以根据pH水平的变化自动注入碱和碳源底物，从而提高发酵工艺操作的可靠性和便利性。
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