公开(公告)号：US20210331172A1

公开(公告)日：2021-10-28

申请号：US17273277

申请日：2019-09-10

Applicant: Sony Corporation

Inventor： Tatsumi Ito ,  Gakuji Hashimoto ,  Kazuya Takahashi ,  Hirotaka Yoshida ,  Yasunobu Kato

IPC: B01L3/00 ,  C12Q1/24 ,  A61K35/14

Abstract: Provided is a technology for preparing a mixture including multiple types of microparticles in accordance with a predetermined ratio. The present technology provides a microparticle sorting device (100) including a control unit (103) comprising a determination unit that determines whether microparticles are sorted, on the basis of light generated by irradiating, with light, the microparticles flowing through a flow channel (155), in which the determination unit performs a primary sorting determination to determine, on the basis of characteristics of the light generated, whether the microparticles belong to any one of two or more different microparticle populations, and then performs a secondary sorting determination to determine whether the microparticles determined to belong to any one of the microparticle populations in the primary sorting determination are sorted, on the basis of the particle constituent ratio specified for the two or more different microparticle populations. This technology may be used to prepare a cell therapeutic agent comprising a mixture of cells of different types in a predefined ratio, e.g. a mixture of various types of CAR T-cells showing a synergistic anti-tumor activity.

公开(公告)号：US20140293273A1

公开(公告)日：2014-10-02

申请号：US14301917

申请日：2014-06-11

Applicant: Sony Corporation

Inventor： Shunpei Suzuki ,  Shingo Imanishi ,  Gakuji Hashimoto ,  Suguru Dowaki

IPC: G01N15/14 ,  G01N21/64

CPC classification number: G01N15/1434 ,  G01N15/1459 ,  G01N21/64 ,  G01N21/645 ,  G01N2015/1493 ,  G01N2021/4716

Abstract: Disclosed herein is an optical measuring device including: a light applying section configured to apply exciting light to a sample flowing in a channel; and a scattered light detecting section configured to detect scattered light generated from the sample irradiated with the exciting light on the downstream side of the sample in the traveling direction of the exciting light; the scattered light detecting section including a scattered light separating mask for separating the scattered light into a low numerical aperture component having a numerical aperture not greater than a specific value and a high numerical aperture component having a numerical aperture greater than the specific value; a first detector for detecting the low numerical aperture component; and a second detector for detecting the high numerical aperture component.

Abstract translation: 本发明公开了一种光学测量装置，包括：光施加部，被配置为向在通道中流动的样品施加激发光; 以及散射光检测部，被配置为检测在激发光的行进方向上在样品的下游侧照射的激光的样品产生的散射光; 散射光检测部分包括用于将散射光分离成具有不大于特定值的数值孔径的低数值孔径分量的散射光分离掩模和具有大于该特定值的数值孔径的高数值孔径分量; 用于检测低数值孔径分量的第一检测器; 以及用于检测高数值孔径分量的第二检测器。

公开(公告)号：US20140144817A1

公开(公告)日：2014-05-29

申请号：US14118994

申请日：2013-02-04

Applicant: Sony Corporation

Inventor： Gakuji Hashimoto ,  Akiko Tsuji ,  Yosuke Muraki ,  Kouhei Hatamoto

IPC: G01N15/14

CPC classification number: G01N15/1425 ,  B01L3/502761 ,  G01N15/14 ,  G01N15/1459 ,  G01N2015/149 ,  Y10S209/908

Abstract: A microparticle sorting device that automatically and accurately adjusts the positions of a fluid stream and a collection container is provided.The microparticle sorting device including a pair of deflecting plates that face each other with a passage area of a fluid stream therebetween, a camera that captures the image of the fluid stream, and a fluid stream detection light source that emits light parallel to a direction in which the deflecting plates face each other and that is movable in a direction perpendicular to the fluid stream and the light is provided. In the microparticle sorting device, the collection container that receives the fluid stream is mounted so as to be movable in the direction perpendicular to the fluid stream and the light.

Abstract translation: 提供了一种自动且准确地调节流体流和收集容器的位置的微粒分选装置。 微粒分选装置包括一对偏转板，它们之间具有流体流的通道面积，相机捕获流体流的图像，以及流体流检测光源，其平行于 偏转板彼此面对并且可以在垂直于流体流的方向上移动，并且提供光。 在微粒分选装置中，接收流体流的收集容器被安装成可以在垂直于流体流和光的方向上移动。

公开(公告)号：USD960740S1

公开(公告)日：2022-08-16

申请号：US29715140

申请日：2019-11-27

Applicant: SONY CORPORATION

Designer： Yuji Akiyama ,  Shoji Akiyama ,  Gakuji Hashimoto ,  Hiroto Kasai ,  Masaya Kakuta ,  Takeshi Yamasaki ,  Tatsumi Ito ,  Masataka Shinoda

公开(公告)号：US12265015B2

公开(公告)日：2025-04-01

申请号：US16976060

申请日：2019-01-28

Applicant: Sony Corporation

Inventor： Gakuji Hashimoto

IPC: G01N15/14 ,  G01N15/10 ,  G01N15/1404 ,  G01N15/149

Abstract: The present technology is to provide a technique for further optimizing microparticle suction conditions, using a microparticle sorting microchip.
The present technology provides a method for optimizing microparticle suction conditions, using a microparticle sorting microchip including: a main flow channel in which a sheath solution and a microparticle-containing sample solution flow; and a pressure chamber that sucks microparticles. The method includes: the step of acquiring data of a velocity V of each microparticle, by introducing the sheath solution and the microparticle-containing sample solution into the main flow channel, and detecting the point of time at which the microparticle passes through a predetermined position in the main flow channel; and the step of controlling the pressure for sucking the microparticles, on the basis of the data of the velocity V of each microparticle.

公开(公告)号：US11169074B2

公开(公告)日：2021-11-09

申请号：US16606518

申请日：2018-02-20

Applicant: SONY CORPORATION

Inventor： Gakuji Hashimoto ,  Tatsumi Ito ,  Kazuya Takahashi ,  Junji Kajihara

IPC: G01N15/14 ,  B01L3/00

Abstract: In the present technology, the timing at which suction is performed is optimized in order to enhance the microparticle separation performance in a technology for separating target microparticles in a microchip. For this purpose, the present technology provides a method of optimizing a microparticle suction condition, and the like, using a microchip having a main flow channel through which a liquid containing a microparticle flows, a microparticle suction flow channel arranged coaxially with the main flow channel, and a branch flow channel branching from the main flow channel. The method includes: a branch point specifying process of specifying a branch point at which the branch flow channel branches from the main flow channel; and a time assignment process of assigning a time T1 to be applied to suction of the microparticle.

公开(公告)号：US10184877B2

公开(公告)日：2019-01-22

申请号：US15423088

申请日：2017-02-02

Applicant: Sony Corporation

Inventor： Gakuji Hashimoto ,  Takayuki Kato

IPC: G01N1/00 ,  G01N15/00 ,  G01N15/14 ,  G01N35/10

Abstract: A sample feeding apparatus includes a first cylinder, a second cylinder, and a sealing section. The first cylinder is configured to be mounted with a sample tube. The second cylinder is configured to move the first cylinder between a first position and a second position, the first position being for mounting of the sample tube, the second position being for feeding of a sample in the sample tube. The sealing section is configured to cover the sample tube, the sample tube being mounted to the first cylinder being at the second position, the first cylinder applying pressure to an inner space of the sealing section at the second position.

公开(公告)号：USD795724S1

公开(公告)日：2017-08-29

申请号：US29561992

申请日：2016-04-21

Applicant: Sony Corporation

Designer： Yuji Akiyama ,  Shoji Akiyama ,  Gakuji Hashimoto ,  Hiroto Kasai ,  Masaya Kakuta ,  Takeshi Yamasaki ,  Tatsumi Ito ,  Masataka Shinoda

公开(公告)号：US08941081B2

公开(公告)日：2015-01-27

申请号：US13939920

申请日：2013-07-11

Applicant: Sony Corporation

Inventor： Shunpei Suzuki ,  Gakuji Hashimoto

IPC: G01J1/58 ,  H01L27/00 ,  G01N15/02 ,  G01N21/00 ,  G01N21/64 ,  G01N15/14 ,  G01N15/10

CPC classification number: G01N21/6486 ,  G01N15/1459 ,  G01N2015/1006 ,  G01N2015/149

Abstract: There is provided a microparticle measurement apparatus including a first light source configured to irradiate excitation light on a droplet containing a microparticle, the droplet being discharged from an orifice, a second light source configured to irradiate illumination light on the droplet for acquiring an image of the droplet, a light receiving element configured to detect fluorescence generated from the microparticle due to the irradiation of the excitation light, and to acquire an image of the droplet, and a filter member configured to be arranged between the droplet and the light receiving element. The filter member includes a first area through which the fluorescence and the illumination light pass, and a second area that is provided around the first area and that has a wavelength selectivity which lets the fluorescence pass through but blocks the illumination light.

Abstract translation: 提供了一种微粒测量装置，其包括：第一光源，被配置为在包含微粒的液滴上照射激发光，所述液滴从孔排出;第二光源，被配置为对所述液滴照射照明光以获取所述微粒的图像 液滴，被配置为检测由于激发光的照射而从微粒产生的荧光并获取液滴的图像的光接收元件，以及被配置为布置在液滴和光接收元件之间的过滤构件。 过滤构件包括荧光和照明光通过的第一区域和设置在第一区域周围并且具有使荧光通过但阻挡照明光的波长选择性的第二区域。

公开(公告)号：US20130309763A1

公开(公告)日：2013-11-21

申请号：US13888056

申请日：2013-05-06

Applicant: SONY CORPORATION

Inventor： Gakuji Hashimoto ,  Takayuki Kato

IPC: G01N1/00 ,  G01N15/00

CPC classification number: G01N15/1404 ,  G01N1/00 ,  G01N15/00 ,  G01N35/1095

Abstract: A sample feeding apparatus includes a first cylinder, a second cylinder, and a sealing section. The first cylinder is configured to be mounted with a sample tube. The second cylinder is configured to move the first cylinder between a first position and a second position, the first position being for mounting of the sample tube, the second position being for feeding of a sample in the sample tube. The sealing section is configured to cover the sample tube, the sample tube being mounted to the first cylinder being at the second position, the first cylinder applying pressure to an inner space of the sealing section at the second position.

Abstract translation: 样品供给装置包括第一气缸，第二气缸和密封部。 第一气缸构造成安装有样品管。 第二气缸构造成在第一位置和第二位置之间移动第一气缸，第一位置用于安装样品管，第二位置用于将样品供给到样品管中。 所述密封部被构造成覆盖所述样品管，所述样品管安装在所述第一气缸的第二位置，所述第一气缸在所述第二位置向所述密封部的内部空间施加压力。