公开(公告)号：US10988786B2

公开(公告)日：2021-04-27

申请号：US15764644

申请日：2016-09-30

Applicant: SYMRISE AG ,  LEIBNIZ-INSTITUT FÜR PFLANZENBIOCHEMIE

Inventor： Ludger A. Wessjohann ,  Susann Herrmann ,  Martin Dippe ,  Torsten Geissler ,  Katrin Geissler ,  Jakob Peter Ley

IPC: C12P17/06 ,  C12N9/02 ,  C12N9/10 ,  C12P7/22 ,  C12P7/26 ,  C12P7/42 ,  C12P17/10

Abstract: The present invention relates to genetically modified enzymes obtained by rational design of the active site binding pocket of the prototypic enzyme 4-hydroxyphenylacetate 3-hydroxylase (4HPA3H) for hydroxylating a 4-hydroxyphenyl compound to yield a 3,4-dihydroxyphenyl compound and to biotechnological methods including in vivo and in vitro methods using said enzymes or catalytically active fragments thereof. Further provided is a method either using a suitable oxidase or hydroxylase further enabling the subsequent site specific methylation of the 3,4-dihydroxyphenyl compound in a coupled enzymatic reaction by providing a suitable O-methyltransferase. Finally, compositions obtainable by the aforementioned methods are disclosed.

公开(公告)号：US10767198B2

公开(公告)日：2020-09-08

申请号：US15779214

申请日：2015-11-28

Applicant: SYMRISE AG

Inventor： Marco A. Fraatz ,  Holger Zorn ,  Johanna Rost ,  Michael Goldmann ,  Egon Gross ,  Jakob Ley ,  Katrin Geissler ,  Torsten Geissler ,  Michael Backes ,  Fabia Hentschel ,  Jens-Michael Hilmer

IPC: C12P7/24 ,  C07C45/27 ,  C12N9/02

Abstract: A method for producing branched aldehydes is proposed, the method comprising the following steps: (a) providing a culture of one or more fungi of the genus Conidiobolus and producing biomass containing branched carboxylic acids in free and/or bound form; (b) extracting the biomass from step (a) to produce a first intermediate containing free and/or bound carboxylic acids; (c) optionally chemically, enzymatically or microbially hydrolyzing the bound carboxylic acids from the first intermediate; (d) treating the first intermediate with a reducing agent of a chemical nature to convert the free and/or bound carboxylic acids into the corresponding alcohols and optionally separating one or more alcohols from interfering by-products and producing the chemically produced second intermediate containing these alcohols as a mixture or in enriched form; (e) treating the first intermediate with a reducing agent of a biological nature to convert the free and/or bound carboxylic acids into the corresponding aldehydes having the same number of carbon atoms compared to the free and/or bound carboxylic acids or into the corresponding aldehydes having a reduced number of carbon atoms by one compared to the free and/or bound carboxylic acids and producing the biologically produced second intermediate containing these aldehydes; (f) treating the chemically produced second intermediate with an oxidizing agent of a chemical nature to convert the free and/or bound alcohols into the corresponding aldehydes; and optionally (g) removing interfering by-components from the fractions obtainable after step(s) (d) and/or (e) and/or (f).

公开(公告)号：US10435673B2

公开(公告)日：2019-10-08

申请号：US15514120

申请日：2015-09-27

Applicant: Symrise AG

Inventor： Ludger Wessjohann ,  Anne-Kathrin Bauer ,  Jakob Ley ,  Torsten Geissler ,  Katrin Geissler

IPC: C12P17/06 ,  C12N9/10 ,  C12N9/02 ,  C12N15/64

Abstract: A method is proposed for producing flavonoids, comprising the steps: (a) providing of a transgenic microorganism, containing (i) a first nucleic acid section (A), comprising or consisting of a gene coding for a CYP450 oxidase, (ii) a second nucleic acid section (B), comprising or consisting of a gene coding for a plant O-methyltransferase, and (b) adding of one or more flavanones to the transgenic microorganism, (c) the conversion of the substrate flavanones by the transgenic microorganism to the corresponding flavonoids, and optionally (d) isolating and purifying of the final products.

公开(公告)号：US20180135029A1

公开(公告)日：2018-05-17

申请号：US15514120

申请日：2015-09-27

Applicant: Symrise AG

Inventor： Ludger Wessjohann ,  Anne-Kathrin Bauer ,  Jakob Ley ,  Torsten Geissier ,  Katrin Geissler

IPC: C12N9/10 ,  C12N9/02 ,  C12N15/64 ,  C12P17/06

CPC classification number: C12N9/1007 ,  C12N9/0071 ,  C12N15/64 ,  C12P17/06 ,  C12Y114/14001 ,  C12Y201/01

Abstract: A method is proposed for producing flavonoids, comprising the steps: (a) providing of a transgenic microorganism, containing (i) a first nucleic acid section (A), comprising or consisting of a gene coding for a CYP450 oxidase, (ii) a second nucleic acid section (B), comprising or consisting of a gene coding for a plant O-methyltransferase, and (b) adding of one or more flavanones to the transgenic microorganism, (c) the conversion of the substrate flavanones by the transgenic microorganism to the corresponding flavonoids, and optionally (d) isolating and purifying of the final products.