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公开(公告)号：US08739992B2
公开(公告)日：2014-06-03
申请号：US13291952
申请日：2011-11-08
申请人： Satoshi Ogata , Kenzo Teshima , Susumu Hirose
发明人： Satoshi Ogata , Kenzo Teshima , Susumu Hirose
CPC分类号： B29C45/14622 , B29C49/06 , B29C2791/001 , B29K2995/0025 , B29K2995/0026
摘要： A multilayered cosmetic container including a hollow inner container including a neck portion, a body portion with a diameter larger than that of the neck portion, and a shoulder portion, which connects the body portion to the neck portion, and a transparent outer layer over-molded on an outer side of the hollow inner container except a part of the neck portion, in which a curvature radius of a shoulder tip, which connects the shoulder portion to the body portion of the hollow inner container, is smaller than the curvature radius of the shoulder tip of the outer layer, which covers the shoulder tip of the hollow inner container, and centers of the curvature radii of the shoulder tip of the hollow inner container and of the shoulder tip of the outer layer do not conform to each other.
摘要翻译： 一种多层化妆品容器，其包括中空内容器，该中空内容器包括颈部，直径大于颈部的本体部分，以及将本体部分连接到颈部的肩部，以及透明外层， 模制在中空内部容器的外侧，除了颈部的一部分之外，其中将肩部与中空内部容器的主体部分连接的肩尖的曲率半径小于 覆盖中空内容器的肩尖的外层的肩尖和中空内容器的肩尖部的曲率半径的中心与外层的肩尖部的中心不一致。
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公开(公告)号：US08858861B2
公开(公告)日：2014-10-14
申请号：US13281755
申请日：2011-10-26
申请人： Satoshi Ogata , Kenzo Teshima , Susumu Hirose
发明人： Satoshi Ogata , Kenzo Teshima , Susumu Hirose
CPC分类号： B29C45/14336 , B29C45/14836 , B29C2045/1445 , B29L2031/7158
摘要： A method for forming an overmolded container by covering a hollow inner bottle made of resin with an injection of a molten resin which forms an outer. The method comprises steps of setting the inner bottle in a mold with a space therebetween for forming the outer and circulating a pressurized gas into and out of a hollow space of the inner bottle through an upper opening thereof. The pressurized gas is supplied through a first path which opens toward an inner wall of the inner bottle and discharged out of the inner bottle through a second path. The pressurized gas supplied into and discharged out of the inner bottle is adjusted in pressure while the molten resin is being injected to the mold.
摘要翻译： 一种用于通过注射形成外部的熔融树脂来覆盖由树脂制成的中空内瓶而形成包覆成型的容器的方法。 该方法包括以下步骤：将内瓶设置在模具中，其间具有用于形成外部的空间，并且将加压气体通过其上部开口循环进入和离开内部瓶的中空空间。 加压气体通过向内瓶的内壁开口的第一路径供给，并通过第二路径排出内瓶。 在将熔融树脂注射到模具中的同时，调节供应到内部瓶子中并从其中排出的加压气体的压力。
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公开(公告)号：US20120118850A1
公开(公告)日：2012-05-17
申请号：US13291952
申请日：2011-11-08
申请人： Satoshi Ogata , Kenzo Teshima , Susumu Hirose
发明人： Satoshi Ogata , Kenzo Teshima , Susumu Hirose
CPC分类号： B29C45/14622 , B29C49/06 , B29C2791/001 , B29K2995/0025 , B29K2995/0026
摘要： A multilayered cosmetic container including a hollow inner container including a neck portion, a body portion with a diameter larger than that of the neck portion, and a shoulder portion, which connects the body portion to the neck portion, and a transparent outer layer over-molded on an outer side of the hollow inner container except a part of the neck portion, in which a curvature radius of a shoulder tip, which connects the shoulder portion to the body portion of the hollow inner container, is smaller than the curvature radius of the shoulder tip of the outer layer, which covers the shoulder tip of the hollow inner container, and centers of the curvature radii of the shoulder tip of the hollow inner container and of the shoulder tip of the outer layer do not conform to each other.
摘要翻译： 一种多层化妆品容器，其包括中空内容器，该中空内容器包括颈部，直径大于颈部的本体部分，以及将本体部分连接到颈部的肩部，以及透明外层， 模制在中空内部容器的外侧，除了颈部的一部分之外，其中将肩部与中空内部容器的主体部分连接的肩尖的曲率半径小于 覆盖中空内容器的肩尖的外层的肩尖和中空内容器的肩尖部的曲率半径的中心与外层的肩尖部的中心不一致。
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公开(公告)号：US5387516A
公开(公告)日：1995-02-07
申请号：US22163
申请日：1993-02-25
申请人： Michiyo Kawai , Shigeharu Mori , Susumu Hirose , Hiroji Tsuji
发明人： Michiyo Kawai , Shigeharu Mori , Susumu Hirose , Hiroji Tsuji
CPC分类号： C12P19/16 , A23L29/35 , C12N9/2451 , C12Y302/01 , Y10S435/832
摘要： A debranching enzyme, a process for producing the enzyme, a microorganism capable of producing the enzyme, and a process for producing glucose liquids using the enzyme are disclosed. The microorganism is preferrably Bacillus FERM BP-4204, the enzyme is a debranching enzyme which acts on alpha-1,6 glucosidase bonds on produce straight chain amylose, acts on pullulan and also acts on relatively long chain saccharides, wherein said substrate specificity for activity on pullulan is set at 100, the activity of said enzyme on soluble starches relative to said activity on pullulan is from about 20 to 40, the activity of said enzyme on glycogen relative to said activity on pullulan is from about 30 to 60, and the activity of said enzyme on amylopectin relative to said activity on pullulan is from about 10 to 30, has an optimum pH from about 4.5 to 6.0, an optimum temperature from about 60.degree. C. to about 70.degree. C., a molecular weight of about 98 kD as measured by SDS-PAGE, and an isoelectric point of about 2.9. The enzyme can be also used for the production of glucose and maltose.
摘要翻译： 公开了脱支酶，生产酶的方法，能够产生酶的微生物以及使用酶生产葡萄糖液的方法。 该微生物优选为芽孢杆菌FERM BP-4204，该酶是起作用的产生直链直链淀粉上的α-1,6糖苷酶键的脱支酶，作用于支链淀粉上，并且还作用于相对长链的糖类，其中所述底物对活性的特异性 在支链淀粉上设定为100，相对于支链淀粉上的所述活性，所述酶对可溶性淀粉的活性为约20至40，所述酶对糖原的活性相对于支链淀粉上的所述活性的活性为约30至60， 所述酶对支链淀粉相对于支链淀粉的活性的活性为约10至30，最佳pH为约4.5至6.0，最适温度为约60℃至约70℃，分子量约为 通过SDS-PAGE测量的98kD，等电点约2.9。 该酶也可用于生产葡萄糖和麦芽糖。
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公开(公告)号：US4902622A
公开(公告)日：1990-02-20
申请号：US100730
申请日：1987-09-25
申请人： Kuniharu Nakai , Masayoshi Shiozaki , Hiroji Tsuji , Shigeharu Mori , Susumu Hirose , Nobumasa Yokoi , Takaichi Ohya
发明人： Kuniharu Nakai , Masayoshi Shiozaki , Hiroji Tsuji , Shigeharu Mori , Susumu Hirose , Nobumasa Yokoi , Takaichi Ohya
IPC分类号： C12N9/44
CPC分类号： C12R1/07 , C12N9/2451 , Y10S435/832
摘要： A novel .alpha.-1,6-glucosidase having the following properties:(1) Action: The enzyme acts on an .alpha.-1,6-glucoside bond to produce linear amylose;(2) Substrate specificity: The enzyme has the following relative activity to polysaccharides:______________________________________ Polysaccharide Relative activity (%) ______________________________________ Pullulan 100 Maize soluble amylopectin 13.5 Soluble starch 6.7 Oyster glycogen 1.7 Hare glycogen 2.6 Maize amylopectin 3.8 Potato amylopectin 4.0 ______________________________________ (3) Km value and Vmax to pullulan: the enzyme has Km value of 0.14 mg/ml and Vmax of 70.0 .mu.mol/min/mg protein;(4) Optimum pH: 5.0 to 5.5;(5) pH stability: The enzyme is stable in the pH range of 4.5 to 6.5 for 30 minutes at 40.degree. C. and in the pH range of 5.0 to 6.0 for 30 minutes at 50.degree. C.;(6) Optimum temperature: Approximately 55.degree. C.;(7) Thermal stability: Treatment at pH 4.5 for 30 minutes at different temperatures indicates that the enzyme retained 80% of the initial activity at 40.degree. C. and 10% thereof at 60.degree. C.;(8) Influence of inhibitor: The enzyme is inhibited by at least 70% by p-chloro-mercuribenzoic acid or sodium dodecylsulfate, but is not inhibited by o-phenanthroline or potassium ferricyanide;(9) Influence of metal salts: The enzyme is negligibly affected by Ni.sup.2+, Ba.sup.2+,Zn.sup.2+, Cd.sup.2+ or Mn.sup.2+ ; is inhibited by Fe.sup.3+ ; and loses activity in the presence of Hg.sup.2+ or Ag.sup.2+ ; and(10) Molecular Weight: About 95,500 (measured by SDS electrophoresis).
摘要翻译： 具有以下性质的新型α-1,6-葡糖苷酶：（1）作用：酶作用于α-1,6葡萄糖苷键以产生线性直链淀粉; （2）底物特异性：该酶对多糖具有以下相对活性： - 多糖相对活性（％） - 支链淀粉100-可溶性支链淀粉13.5-可溶淀粉6.7-牡蛎糖原1.7-高糖糖原2.6-支链淀粉3.8-支链淀粉 4.0 - （3）Km值和Vmax至支链淀粉：酶的Km值为0.14mg / ml，Vmax为70.0μmol/ min / mg蛋白质; （4）最适pH：5.0〜5.5; （5）pH稳定性：该酶在40℃，pH5.0〜6.0的pH范围为4.5〜6.5，在50℃，pH为5.0〜6.0，稳定30分钟。 （6）最佳温度：约55℃。 （7）热稳定性：在不同温度下在pH 4.5下处理30分钟表明酶在40℃下保留80％的初始活性，在60℃下保留10％的初始活性。 （8）抑制剂的影响：该酶被对氯 - 汞二苯甲酸或十二烷基硫酸钠抑制至少70％，但不被邻菲咯啉或铁氰化钾抑制; （9）金属盐的影响：酶被Ni2 +，Ba2 +，Zn2 +，Cd2 +或Mn2 +忽略不计; 被Fe3 +抑制; 并在Hg2 +或Ag2 +存在下失去活性; 和（10）分子量：约95,500（通过SDS电泳测量）。
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公开(公告)号：US5252469A
公开(公告)日：1993-10-12
申请号：US750160
申请日：1991-08-26
申请人： Hiroyasu Andou , Akira Matsuura , Susumu Hirose
发明人： Hiroyasu Andou , Akira Matsuura , Susumu Hirose
CPC分类号： C12N9/1044 , C12R1/56
摘要： A process for producing a transglutaminase which comprises (1) culturing a microorganism having the identifying characteristics of a microorganism selected from the group consisting of Streptomyces sp. No. 83, deposited as FERM BP-3505, Streptomyces lavendulae No. 466, deposited as FERM BP-3506, and mutants thereof, wherein the microorganism is capable of producing a transglutaminase, wherein the transglutaminase is an enzyme which catalyzes an acyl transfer reaction of a .gamma.-carboxyamide group of a glutamine residue in a peptide or protein chain in the presence or absence of Ca.sup.++ and wherein the transglutaminase has an isoelectric point of 10 or 6.8, respectively, and is inhibited by Pb ions, and (2) recovering the transglutaminase from the culture obtained is disclosed.
摘要翻译： 一种转谷氨酰胺酶的制造方法，其特征在于，（1）培养具有选自由链霉菌属（Streptomyces sp。 以作为FERM BP-3506保藏的FERM BP-3505，粟酒链霉菌466和其突变体保藏的第83号，其中微生物能够产生转谷氨酰胺酶，其中转谷氨酰胺酶是催化酰基转移反应的酶 的存在或不存在Ca ++的肽或蛋白质链中的谷氨酰胺残基的γ-羧酰胺基团，其中转谷氨酰胺酶的等电点分别为10或6.8，并被Pb离子抑制，和（2 ）从所获得的培养物中回收转谷氨酰胺酶。
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公开(公告)号：US07238857B2
公开(公告)日：2007-07-03
申请号：US10503261
申请日：2003-02-06
申请人： Ken-ichi Yamazaki , Susumu Hirose
发明人： Ken-ichi Yamazaki , Susumu Hirose
CPC分类号： C07K14/415 , C12N15/8238
摘要： An ethylene-responsive transcription co-activator (MBF) acts on ethylene-responsive transcription factors (ERFs), which positively controls the expression of ethylene-responsive plant genes. A plant is transformed by the gene of the co-activator, and a promoter that may increase or decrease responsiveness to ethylene depending on its alignment. A method for controlling the responsiveness of plants to ethylene transforms a plant comprising an ERF-dependent gene, which positively control the expression of ethylene responsive genes of plants. This transcription co-activator gene is applied to control ethylene response of plants.
摘要翻译： 乙烯应答转录共激活子（MBF）作用于乙烯应答转录因子（ERFs），其正向控制乙烯应答植物基因的表达。 植物由共活化剂的基因转化，并且可以根据其对准而增加或减少对乙烯的响应性的启动子。 用于控制植物对乙烯的响应性的方法将包含ERF依赖性基因的植物转化，所述基因能正确控制植物的乙烯应答基因的表达。 该转录共活化基因用于控制植物的乙烯应答。
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公开(公告)号：US20050132443A1
公开(公告)日：2005-06-16
申请号：US10503261
申请日：2003-02-06
申请人： Ken-ichi Yamazaki , Susumu Hirose
发明人： Ken-ichi Yamazaki , Susumu Hirose
CPC分类号： C07K14/415 , C12N15/8238
摘要： The purpose of the present invention is to identify a transcription coactivator (transcriptional cofactor, MBF) in ethylene-responsive transcription factors (ERFs), to elucidate the mechanism of the action of MBF to ERF, which positively controls the expression of ethylene-responsive genes of plants, and furthermore, to provide a method for controlling the ethylene response of plants. An ethylene-responsive transcription coactivator (SEQ ID NO: 1 and 2) was identified. The transcription coactivator is demonstrated to be a transcription coactivator for ERF, which positively control the expression of ethylene-responsive genes of plants. This transcription coactivator gene can be applied to control ethylene response of plants.
摘要翻译： 本发明的目的是鉴定乙烯应答转录因子（ERFs）中的转录共激活因子（转录辅因子，MBF），以阐明MBF对ERF的作用的机制，该功能正向控制乙烯应答基因的表达 的植物，此外，提供一种控制植物的乙烯应答的方法。 鉴定了乙烯应答转录共激活因子（SEQ ID NO：1和2）。 转录共激活因子被证明是ERF的转录共激活因子，其能正确控制植物乙烯应答基因的表达。 该转录共激活子基因可用于控制植物的乙烯应答。
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