公开(公告)号：US20180216179A1

公开(公告)日：2018-08-02

申请号：US15886533

申请日：2018-02-01

Applicant: SEAGATE TECHNOLOGY LLC

Inventor： Xiaomin YANG ,  ShuaiGang XIAO ,  David S. KUO ,  Koichi WAGO ,  Thomas Young CHANG

IPC: C12Q1/6869 ,  C12M1/34

CPC classification number: C12Q1/6869 ,  B82Y15/00 ,  C12M1/34 ,  C12Q2563/159 ,  C12Q2565/607 ,  C12Q2565/629

Abstract: Apparatus and methods relating to DNA sequencing are provided. In one embodiment, a DNA sequencing device includes a nanochannel having a width that is approximately 0.3 nm to approximately 20 nm. A pair of electrodes having portions exposed to the nanochannel may form a tunneling current electrode (TCE) with an electrode gap of approximately 0.1 nm to approximately 2 nm, and more particularly about 0.3 nm to about 1 nm. In one embodiment, at least one of the pair of electrodes is formed as a suspended electrode. An actuator may be associated with the suspended electrode to displace it relative to the other electrode. In various embodiments, the nanochannel and/or the electrodes may be formed using thermal reflow processes to reduce the size of such features.

公开(公告)号：US20180259475A1

公开(公告)日：2018-09-13

申请号：US15886723

申请日：2018-02-01

Applicant: SEAGATE TECHNOLOGY LLC

Inventor： ShuaiGang XIAO ,  David S. KUO ,  Xiaomin YANG ,  Kim Yang LEE ,  Yautzong HSU ,  Michael R. FELDBAUM

IPC: G01N27/327 ,  C12Q1/6869 ,  G01N33/487 ,  B82Y5/00

CPC classification number: G01N27/3278 ,  B82Y5/00 ,  C12Q1/6869 ,  G01N33/48721 ,  C12Q2563/116 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: A DNA sequencing device, and related method, which include a nanopore having a maximum width dimension of no greater than about 50 nm, and a pair of electrodes having a spacing of no greater than about 2 nm, the electrodes being exposed within the nanopore to measure a DNA strand passing through the nanopore.

公开(公告)号：US20210115508A1

公开(公告)日：2021-04-22

申请号：US17137837

申请日：2020-12-30

Applicant: Seagate Technology LLC

Inventor： Xiaomin YANG ,  ShuaiGang XIAO ,  David S. KUO ,  Koichi WAGO ,  Thomas Young CHANG

IPC: C12Q1/6869 ,  C12M1/34

Abstract: Apparatus and methods relating to DNA sequencing are provided. In one embodiment, a DNA sequencing device includes a nanochannel having a width that is approximately 0.3 nm to approximately 20 nm. A pair of electrodes having portions exposed to the nanochannel may form a tunneling current electrode (TCE) with an electrode gap of approximately 0.1 nm to approximately 2 nm, and more particularly about 0.3 nm to about 1 nm. In one embodiment, at least one of the pair of electrodes is formed as a suspended electrode. An actuator may be associated with the suspended electrode to displace it relative to the other electrode. In various embodiments, the nanochannel and/or the electrodes may be formed using thermal reflow processes to reduce the size of such features.

公开(公告)号：US20200240947A1

公开(公告)日：2020-07-30

申请号：US16845565

申请日：2020-04-10

Applicant: SEAGATE TECHNOLOGY LLC

Inventor： ShuaiGang XIAO ,  David S. KUO ,  Xiaomin YANG ,  Kim Yang LEE ,  Yautzong HSU ,  Michael R. FELDBAUM

IPC: G01N27/327 ,  C12Q1/6869 ,  G01N33/487

Abstract: A DNA sequencing device and methods of making. The device includes a pair of electrodes having a spacing of no greater than about 2 nm, the electrodes being exposed within a nanopore to measure a DNA strand passing through the nanopore. The device can be made by depositing a conductive layer over a sacrificial channel and then removing the sacrificial channel to form the electrode gap.

公开(公告)号：US20200249195A1

公开(公告)日：2020-08-06

申请号：US16854607

申请日：2020-04-21

Applicant: SEAGATE TECHNOLOGY LLC

Inventor： David S. KUO ,  Xiaomin YANG ,  ShuaiGang XIAO ,  Kim Yang LEE ,  Koichi R. WAGO ,  Thomas Young CHANG

IPC: G01N27/327 ,  C12Q1/6869 ,  G01N33/487 ,  B01L3/00 ,  B81C1/00

Abstract: A DNA sequencing device, and related methods, include a nanopore or nanochannel structure, and a nanoelectrode. The nanoelectrode includes electrode members having free ends exposed within the nanopore or nanochannel structure, an electrode gap defined between of the free ends, and plated portions formed on the free ends to provide a reduced sized for the electrode gap.

公开(公告)号：US20180217083A1

公开(公告)日：2018-08-02

申请号：US15886581

申请日：2018-02-01

Applicant: SEAGATE TECHNOLOGY LLC

Inventor： David S. KUO ,  Xiaomin YANG ,  ShuaiGang XIAO ,  Kim Yang LEE ,  Koichi WAGO ,  Thomas Young CHANG

IPC: G01N27/327 ,  B01L3/00 ,  B81C1/00

CPC classification number: G01N27/3278 ,  B01L3/502707 ,  B01L3/502715 ,  B81C1/00547 ,  B82Y5/00 ,  C12Q1/6809 ,  C12Q1/6869 ,  G01N33/48721 ,  C12Q2565/631

Abstract: A DNA sequencing device, and related methods, include a nanopore or nanochannel structure, and a nanoelectrode. The nanoelectrode includes electrode members having free ends exposed within the nanopore or nanochannel structure, an electrode gap defined between of the free ends, and plated portions formed on the free ends to provide a reduced sized for the electrode gap.

公开(公告)号：US20180216169A1

公开(公告)日：2018-08-02

申请号：US15886442

申请日：2018-02-01

Applicant: SEAGATE TECHNOLOGY LLC

Inventor： Koichi WAGO ,  ShuaiGang XIAO ,  Xiaomin YANG ,  Kim Yang LEE ,  David S. KUO ,  Thomas Young CHANG

IPC: C12Q1/6825 ,  C12Q1/6874 ,  G01N27/447 ,  G01N33/487 ,  C12Q1/6837 ,  G01N27/414 ,  C12Q1/6806 ,  B01L3/00

CPC classification number: C12Q1/6825 ,  B01L3/502761 ,  C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N27/44791 ,  G01N33/48721 ,  C12Q2565/631

Abstract: Apparatus and methods to sequence DNA. A DNA sequencing device includes a passage, a first electrode, and a second electrode. The passage has a width and a length. The first and second electrodes are exposed within the passage and spaced apart from each other to form an electrode gap. The electrode gap is no greater than about 2 nm. The DNA sequencing device is operable to measure with the first and second electrodes a change in electronic signal in response to nucleotides of a DNA strand passing through the electrode gap.

公开(公告)号：US20200255895A1

公开(公告)日：2020-08-13

申请号：US16862314

申请日：2020-04-29

Applicant: SEAGATE TECHNOLOGY LLC

Inventor： Kim Yang LEE ,  David S. KUO ,  Thomas Young CHANG ,  Xiaomin YANG ,  ShuaiGang XIAO ,  Koichi WAGO

IPC: C12Q1/6874 ,  G01N27/447 ,  G01N33/487

Abstract: A DNA sequencing device, and related methods, include a nanochannel sized to receive a DNA strand, a first electrode member exposed within the nanochannel, and a second electrode member exposed within the nanochannel and spaced apart from the first electrode to form an electrode gap. The second electrode member has a wedge shaped profile, and the first and second electrode members are operable to detect a change in electronic signal as the DNA strand passes through the electrode gap.

公开(公告)号：US20180245149A1

公开(公告)日：2018-08-30

申请号：US15886560

申请日：2018-02-01

Applicant: SEAGATE TECHNOLOGY LLC

Inventor： ShuaiGang XIAO ,  David S. KUO ,  Kim Yang LEE ,  Xiaomin YANG ,  Koichi WAGO ,  Thomas Young CHANG

IPC: C12Q1/6874 ,  G06F19/22 ,  G01N27/447 ,  B82Y40/00

CPC classification number: C12Q1/6874 ,  B82Y15/00 ,  B82Y40/00 ,  C12Q1/6869 ,  G01N27/44791 ,  G16B30/00 ,  C12Q2565/631

Abstract: A nanochannel DNA sequencing device and related methods of fabrication and of DNA sequencing are provided. In one example, a device may include a nanochannel having a width of no greater than about 2 nm and a height no greater than 1.5 times the width. The device may further include a pair of electrodes having a width of no greater than about 10 nm, the electrodes being exposed within the nanochannel to measure electronical characteristics of a DNA strand passing through the nanochannel. In one example, the nanochannel may be formed using lithography techniques, such as sidewall lithography.

公开(公告)号：US20180217084A1

公开(公告)日：2018-08-02

申请号：US15886685

申请日：2018-02-01

Applicant: SEAGATE TECHNOLOGY LLC

Inventor： David S. KUO ,  ShuaiGang XIAO ,  Kim Yang LEE ,  Xiaomin YANG ,  Koichi WAGO ,  Thomas Young CHANG

IPC: G01N27/327 ,  G01N33/487 ,  G01N33/543 ,  C12Q1/6869 ,  B82Y5/00 ,  B82Y40/00

CPC classification number: G01N27/3278 ,  B82Y5/00 ,  B82Y15/00 ,  B82Y40/00 ,  C12Q1/6869 ,  G01N33/48721 ,  G01N33/5438

Abstract: A DNA sequencing device includes a first layer having a nanochannel formed therein, and a pair of electrodes arranged vertically relative to each other and spaced apart to define an electrode gap. The electrode gap is exposed in the nanochannel, and the electrode gap is in the range of about 0.3 nm to about 2 nm.