公开(公告)号：US5286627A

公开(公告)日：1994-02-15

申请号：US510716

申请日：1990-04-18

申请人： Shigeru Ueda ,  Masashi Tanno ,  Hideo Misaki

发明人： Shigeru Ueda ,  Masashi Tanno ,  Hideo Misaki

IPC分类号： C12Q1/32 ,  C12Q1/60 ,  C12N9/02 ,  C12N9/08 ,  C12Q1/26

CPC分类号： C12Q1/32 ,  Y10S435/81

摘要： A method for a high-sensitivity quantitative analysis of bile acid in a bile acid-containing sample utilizes a reagent comprising:(1) a steroid dehydrogenase which is capable of effecting a reversible reaction producing oxobile acid using bile acid, as a substrate, and a nicotinamide adenine dinucleotide phosphate compound (hereinafter referred to as an NADP compound) or a nicotinamide adenine dinucleotide compound (hereinafter referred to as an NAD compound) as coenzyme;(2) a compound A.sub.1 selected from the group consisting of NADP compounds and NAD compounds, in an amount surplus relative to the amount of bile acid;(3)(i) compound B.sub.1 selected from the group consisting of a reduced NAD compound and a reduced NADP compound, compound B.sub.1 being a reduced NAD compound, when A.sub.1 is an NADP compound, and a reduced NADP compound, when A.sub.1 is an NAD compound, or(ii) a compound B.sub.2 which is an oxidized product of B.sub.1, or(iii) a mixture of B.sub.1 and B.sub.2, where the amount of B.sub.1 plus B.sub.2 is less than 1/100 of the molar amount of A.sub.1, and(iv) a second dehydrogenase which does not react with bile acid and compound A.sub.1, but does effect a reaction converting compound B.sub.2 into compound B.sub.1, and the substrate of the second dehydrogenase.

摘要翻译： 用于胆汁酸样品中胆汁酸的高灵敏度定量分析的方法使用试剂，其包括：（1）能够实现使用胆汁酸作为底物产生氧化酸的可逆反应的类固醇脱氢酶，以及 烟酰胺腺嘌呤二核苷酸磷酸化合物（以下称为NADP化合物）或烟酰胺腺嘌呤二核苷酸化合物（以下称为NAD化合物）作为辅酶; （2）选自NADP化合物和NAD化合物的化合物A1，相对于胆汁酸量为过量; （3）（A1）当NAD为NAD的化合物时，当A1为NADP化合物时，化合物B1为还原的NAD化合物，还原的NADP化合物为化合物B1，还原的NADP化合物为NAD 化合物，或（ii）作为B1的氧化物的化合物B2或（iii）B1和B2的混合物，其中B1加B2的量小于A1摩尔量的1/100，和（ iv）不与胆汁酸和化合物A1反应的第二脱氢酶，但是将化合物B2转化为化合物B1和将第二脱氢酶的底物进行反应。

公开(公告)号：US4791057A

公开(公告)日：1988-12-13

申请号：US757953

申请日：1985-07-23

申请人： Hideo Misaki ,  Shigeru Ueda

发明人： Hideo Misaki ,  Shigeru Ueda

IPC分类号： G01N33/50 ,  C12Q1/26 ,  C12Q1/28 ,  C12Q1/32 ,  G01N33/74 ,  G01N33/92

CPC分类号： G01N33/92 ,  C12Q1/26 ,  C12Q1/32 ,  G01N33/743 ,  Y10S436/817

摘要： A highly sensitive quantitative assay method for a component which is 3.beta.-hydroxysteroid or 3-ketosteroid, in a specimen to be assayed, comprising causing this component in the specimen to take part in the cycling reaction ##STR1## and measuring a detectable change in the reaction system. There is thus provided a 3.beta.-hydroxysteroid - 3-ketosteroid cycling reaction using 3.beta.-hydroxysteroid oxidase, which consumes O.sub.2 and generates H.sub.2 O.sub.2 and 3-ketosteroid, with a substrate of 3.beta.-hydroxysteroid, or 3.beta.-hydroxysteroid dehydrogenase, which consumes reduced NAD(P) and generate NAD(P) and 3.beta.-hydroxysteroid, with a substrate of 3-ketosteroid. Example of specimens are specimens which contain 3-hydroxysteroid or 3-ketosteroid, or which liberate or generate such a component. By proceeding at a rate of more than ten cycles per minute and measuring the amount of a detectable change in the reaction, the component in a specimen can easily and sensitively be detected with good accuracy.

摘要翻译： 在待测样品中的3β-羟基类固醇或3-酮甾类成分的高度灵敏的定量测定方法，包括使样品中的该组分参与循环反应＆IMAGE>并测量可检测的变化 反应体系。 因此，提供了使用3种β-羟基类固醇氧化酶的3β-羟基甾类-3-酮类固醇循环反应，其消耗O 2并产生H 2 O 2和3-酮甾类，具有3β-羟基类固醇或3β-羟基类固醇脱氢酶的底物，其中 消耗NAD（P）并产生NAD（P）和3β-羟基类固醇，与3-酮类固醇的底物。 样品的实例是含有3-羟基类固醇或3-酮甾类的样品，或释放或产生这样的组分。 通过以每分钟超过10个循环的速率进行测量并测量反应中可检测的变化量，可以以高精度容易且灵敏地检测样品中的组分。

公开(公告)号：US5780256A

公开(公告)日：1998-07-14

申请号：US108736

申请日：1995-03-13

申请人： Shigeru Ueda ,  Mamoru Takahashi ,  Hideo Misaki ,  Shigeru Ikuta

发明人： Shigeru Ueda ,  Mamoru Takahashi ,  Hideo Misaki ,  Shigeru Ikuta

IPC分类号： C12Q1/32

CPC分类号： C12Q1/32

摘要： The present invention is directed to a method for the quantitative determination of ammonia, an .alpha.-amino acid and an .alpha.-keto acid corresponding to the .alpha.-amino acid, or a chemical substance producing any one of these compounds. The present invention is also directed to an analytical composition for use in the above method. The method of the present invention ensures rapidness and accuracy in the determination of ammonia, .alpha.-amino acids or .alpha.-keto acids, even with the use of a small quantity of a biological sample. This method is very useful in application fields, such as clinical diagnosis and food testing.

摘要翻译： PCT No.PCT / JP91 / 01785 Sec。 371日期1995年3月13日 102（e）1995年3月13日PCT PCT 1991年12月27日PCT公布。 WO92 / 15705 PCT公开号 日期1992年9月17日本发明涉及一种定量测定对应于α-氨基酸的氨，α-氨基酸和α-酮酸的方法，或者是产生这些化合物中的任何一种的化学物质。 本发明还涉及用于上述方法的分析组合物。 本发明的方法即使使用少量的生物样品也能确保氨，α-氨基酸或α-酮酸的测定的快速和准确性。 该方法在临床诊断和食品检测等应用领域非常有用。

公开(公告)号：US5356790A

公开(公告)日：1994-10-18

申请号：US106693

申请日：1993-08-16

申请人： Shigeru Ueda ,  Mamoru Takahashi ,  Hideo Misaki ,  Shigeyuki Imamura ,  Kazuo Matsuura

发明人： Shigeru Ueda ,  Mamoru Takahashi ,  Hideo Misaki ,  Shigeyuki Imamura ,  Kazuo Matsuura

IPC分类号： C12N9/04 ,  C12Q1/02 ,  C12Q1/32 ,  C12Q1/26 ,  C07H1/00 ,  C12N11/00

CPC分类号： C12N9/0006 ,  C12Q1/02 ,  C12Q1/32 ,  C12R1/07 ,  Y10S436/817

摘要： Myo-inositol in a specimen is assayed by reacting a specimen containing myo-inositol with:a) myo-inositol dehydrogenase using a thio-NADP group or thio-NAD group and an NADP group or NAD group as coenzymes, and which catalyzes a reversible reaction forming myo-inosose from myo-inositol,b ) A.sub.1 andc) B.sub.1to effect a cycling reaction ##STR1## wherein A.sub.1 is a thio-NADP group, thio-NAD group, NADP group or NAD group, A.sub.2 is a reduced form of A.sub.1, when A.sub.1 is a thio-NADP group or thio-NAD group, B.sub.1 is a reduced NADP group or reduced NAD group and when A.sub.1 is an NADP group or NAD group, B.sub.1 is a reduced thio-NADP group or reduced thio-NAD group, and wherein B.sub.2 is an oxidized form of B.sub.1. The change in the amount of A.sub.2 generated or B.sub.1 consumed by the cycling reaction is measured to perform the assay. A composition for performing the assay comprises the above myo-inositol dehydrogenase, as well as the above components A.sub.1 and B.sub.1. The myo-inositol dehydrogenase can be produced by culturing a suitable microorganism belonging to genus Bacillus, particularly Bacillus sp. No. 3 FERM BP-3013.

摘要翻译： 通过使含有肌醇的样品与以下物质反应来测定样品中的肌醇：a）使用硫代NADP基团或硫代NAD基团和NADP基团或NAD基团作为辅酶的肌醇脱氢酶，并催化可逆的 由肌球蛋白形成肌内单糖的反应，b）A1和c）B1进行循环反应，其中A1是硫代NADP基团，硫代NAD基团，NADP基团或NAD基团，A2是还原形式 的A1，当A1是硫代NADP基团或硫代NAD基团时，B1是还原的NADP基团或还原的NAD基团，当A1是NADP基团或NAD基团时，B1是还原的硫代NADP基团或还原的硫代 - NAD基，其中B2是B1的氧化形式。 测量由循环反应消耗的A2产生量或B1的量的变化以进行测定。 用于进行测定的组合物包含上述肌醇脱氢酶，以及上述组分A1和B1。 肌醇脱氢酶可以通过培养属于芽孢杆菌属（Bacillus），特别是芽孢杆菌属（Bacillus sp。）的合适的微生物来产生。 3号FERM BP-3013。

公开(公告)号：US5122454A

公开(公告)日：1992-06-16

申请号：US549538

申请日：1990-07-09

申请人： Shigeru Ueda ,  Hideo Misaki ,  Shigeyuki Imamura

发明人： Shigeru Ueda ,  Hideo Misaki ,  Shigeyuki Imamura

IPC分类号： G01N33/50 ,  C12Q1/44 ,  C12Q1/48 ,  C12Q1/60

CPC分类号： C12Q1/60 ,  Y10S435/81

摘要： An assay with high sensitivity for activity of lecithin-cholesterol acyltransferase in blood for functional analysis of liver, by bringing the blood into contact with lecithin and free cholesterol until lysolecithin and cholesterol ester are produced, allowing the lysolecithin produced to react with lysophospholipase and glycerophosphocholine phosphodiesterase and assaying glycerol-3-phosphate produced simultaneously or successively in the reaction by means of an enzymatic cycling reaction in which glycerol-3-phosphate, dihydroxyacetone-3-phosphate, nicotinamide adenine dinucleotide (NAD), reduced NAD, O.sub.2, H.sub.2 O.sub.2, glycerophosphate oxidase and glycerophosphate dehydrogenase take part.

摘要翻译： 通过使血液与卵磷脂和游离胆固醇接触直到溶血卵磷脂和胆固醇酯，产生对血液中卵磷脂 - 胆固醇酰基转移酶活性的高灵敏度检测，用于功能分析肝脏，使溶解卵磷脂产生的溶血卵磷脂与溶血磷脂酶和甘油磷酸胆碱磷酸二酯酶 并通过其中甘油-3-磷酸，二羟基丙酮-3-磷酸，烟酰胺腺嘌呤二核苷酸（NAD），还原的NAD，O 2，H 2 O 2，甘油磷酸酯的酶循环反应测定反应中同时或相继产生的甘油-3-磷酸 氧化酶和甘油磷酸脱氢酶参与。

公开(公告)号：US5117467A

公开(公告)日：1992-05-26

申请号：US714368

申请日：1991-06-12

申请人： Hideo Misaki ,  Shigeru Ueda ,  Kazuhiro Watanabe ,  Yuzo Ishikawa ,  Hirao Nagae ,  Takashi Matsuzawa

发明人： Hideo Misaki ,  Shigeru Ueda ,  Kazuhiro Watanabe ,  Yuzo Ishikawa ,  Hirao Nagae ,  Takashi Matsuzawa

IPC分类号： C12M1/34 ,  C12Q1/06 ,  G06T1/00 ,  G06T7/60

CPC分类号： C12M41/36 ,  C12Q1/06

摘要： A colony counting apparatus includes a reading unit with a visual sensor incorporated therein. The presence of a colony is detected at the end of that colony by moving the reading unit along lines selected at an appropriate pitch and by reading data on the individual points set along each line as logical high or low signals. The values obtained in the above-described counting operation are added and stored in an addition/storage device in a CPU, and the results of addition which are stored in the addition/storage device are output to a suitable display by an outputting device when the counting operation is completed.

摘要翻译： 集落计数装置包括其中包含视觉传感器的读取单元。 通过沿着以适当间距选择的线移动读取单元，并通过读取沿着每行设置的各个点的数据作为逻辑高或低信号，在该殖民地结束时检测到菌落的存在。 将上述计数操作中获得的值相加并存储在CPU中的加法/存储装置中，并且存储在加法/存储装置中的相加结果通过输出装置输出到合适的显示器 计数操作完成。

公开(公告)号：US5633143A

公开(公告)日：1997-05-27

申请号：US244450

申请日：1994-05-26

申请人： Shigeru Ueda ,  Hideo Misaki ,  Shigeru Ikuta ,  Mamoru Takahashi

发明人： Shigeru Ueda ,  Hideo Misaki ,  Shigeru Ikuta ,  Mamoru Takahashi

IPC分类号： C12Q1/32 ,  C12Q1/52 ,  G01N31/00 ,  G01N33/53

CPC分类号： C12Q1/32 ,  Y10S435/966 ,  Y10S435/973 ,  Y10T436/104998

摘要： Disclosed is a method for the quantitative determination of D-3-hydroxybutyric acid and acetoacetic acid, which comprises reacting a biological sample containing D-3-hydroxybutyric acid and acetoacetic acid, with a reagent comprising: (1) a D-3-hydroxybutyrate dehydrogenase, (2) A.sub.1 and (3) B.sub.1, the components (1), (2) and (3) participating in the following cycling reaction: ##STR1## thereby effecting the enzymatic cycling reaction, and measuring a change in the amount of A.sub.2 formed or the amount of B.sub.1 consumed. Also disclosed is an analytical reagent comprising the components (1), (2) and (3) for use in the above method. The method and the analytical reagent ensure rapidness and accuracy in the determination of D-3-hydroxybutyric acid and acetoacetic acid, even with the use of a small quantity of a biological sample, so that they are very useful in application fields, such as clinical diagnosis and food testing.

摘要翻译： PCT No.PCT / JP91 / 01706 Sec。 371日期：1994年5月26日 102（e）日期1994年5月26日PCT 1991年12月12日PCT PCT。 公开号WO93 / 12254 日期：1993年6月24日公开是定量测定D-3-羟基丁酸和乙酰乙酸的方法，其包括使含有D-3-羟基丁酸和乙酰乙酸的生物样品与包含以下物质的试剂反应：（1） D-3-羟基丁酸脱氢酶，（2）A1和（3）B1，参与以下循环反应的组分（1），（2）和（3）：进行酶循环反应，并测量 形成的A2的量的变化或消耗的B1的量。 还公开了包含用于上述方法的组分（1），（2）和（3）的分析试剂。 该方法和分析试剂确保即使使用少量生物样品也能快速，准确地测定D-3-羟基丁酸和乙酰乙酸，因此它们在临床应用领域非常有用 诊断和食品检测。

公开(公告)号：US20050146749A1

公开(公告)日：2005-07-07

申请号：US11074703

申请日：2005-03-09

申请人： Shigeru Ueda

发明人： Shigeru Ueda

IPC分类号： B41J29/38 ,  G06K15/00 ,  H04N1/00 ,  H04N1/04 ,  H04N1/32 ,  G06F15/00 ,  H04N1/23

CPC分类号： H04N1/00912 ,  G06K15/00 ,  H04N1/00915 ,  H04N1/32358 ,  H04N1/32496 ,  H04N1/32561 ,  H04N2201/0081 ,  H04N2201/0082 ,  H04N2201/0087

摘要： An image input-output apparatus is provided with a printing function for printing an image specified by print data from an external device and a reading function for reading an original image. The apparatus includes a printing function realizing unit, a discrimination unit, and a control unit. The printing function realizing unit operates in a first mode for realizing the printing function with a relatively small memory capacity and operates in a second mode for realizing the printing function with a relatively large memory capacity. The discrimination unit discriminates whether printing of the image specified by the print data is to be realized by the first mode or the second mode. If it is discriminated that printing is to be realized by the first mode, the control unit is adapted to allow parallel execution of the printing function and the reading function. Otherwise, parallel execution is inhibited.

摘要翻译： 图像输入输出装置具有打印功能，用于打印来自外部设备的打印数据指定的图像以及用于读取原始图像的读取功能。 该装置包括打印功能实现单元，鉴别单元和控制单元。 打印功能实现单元以实现具有相对较小存储容量的打印功能的第一模式操作，并以第二模式操作，以实现具有相对较大存储容量的打印功能。 鉴别单元鉴别是否通过第一模式或第二模式来实现由打印数据指定的图像的打印。 如果区别是要通过第一模式实现打印，则控制单元适于允许并行执行打印功能和读取功能。 否则，并行执行被禁止。

公开(公告)号：US06449058B1

公开(公告)日：2002-09-10

申请号：US09309496

申请日：1999-05-11

申请人： Shigeru Ueda

发明人： Shigeru Ueda

IPC分类号： B41B000

CPC分类号： G06K15/00 ,  G06F3/12 ,  G06K15/1814 ,  G06K15/1865 ,  G06K2215/0011 ,  G06K2215/0014

摘要： Print data including text or graphics data is transferred from a host computer to a printer. The printer develops the received data on a band basis. When data processing to be performed by the printer is expected to fail due to shortage of the memory area size, the host computer cancels the data which has already been sent to the printer, and develops the text or graphics data to form image data. The image data thus formed is sent to the printer. Image data is transferred in a hierarchical manner. Disclosed also is a method which makes it possible to eliminate as much as possible any data processing failure in the printer attributable to shortage of memory capacity.

摘要翻译： 包括文本或图形数据的打印数据从主机传输到打印机。 打印机以带为基础开发接收到的数据。 当打印机执行的数据处理预期由于存储区域大小不足而失败时，主计算机取消已经发送到打印机的数据，并且显影文本或图形数据以形成图像数据。 这样形成的图像数据被发送到打印机。 图像数据以分层方式传送。 还公开了一种方法，其可以尽可能地消除由于存储器容量不足导致的打印机中的任何数据处理故障。

公开(公告)号：US06373581B1

公开(公告)日：2002-04-16

申请号：US08600050

申请日：1996-02-12

申请人： Junichi Kimizuka ,  Shigeru Ueda ,  Satoshi Nagata ,  Akihiro Nakamura ,  Yoji Kaneko ,  Soya Endo ,  Satoshi Egawa ,  Yoichi Toyokura ,  Hitoshi Machino ,  Shinichiro Maekawa ,  Yutaka Tokura ,  Masaki Unishi ,  Akira Nakaya

发明人： Junichi Kimizuka ,  Shigeru Ueda ,  Satoshi Nagata ,  Akihiro Nakamura ,  Yoji Kaneko ,  Soya Endo ,  Satoshi Egawa ,  Yoichi Toyokura ,  Hitoshi Machino ,  Shinichiro Maekawa ,  Yutaka Tokura ,  Masaki Unishi ,  Akira Nakaya

IPC分类号： G06F1500

CPC分类号： G06K15/16 ,  G06K15/1805

摘要： If an optional paper feeding deck or furnisher, or paper stacker, is connected to an image forming apparatus (such as a printer), such optional devices are to be controlled by connecting the paper conveyance control apparatus to control those options. Such an image forming apparatus has a video controller to convert the printer language sent from a high-order device such as a host computer, into bit map data and a printer engine to print the bit map data. Such a paper conveyance control apparatus, being electrically connected to the video controller, has a memory to be shared with the video controller. The video controller writes the job schedule in the shared memory. The paper conveyance control apparatus controls the optional device according to the written job schedule.

摘要翻译： 如果可选的送纸台或家具或纸堆纸器连接到图像形成装置（例如打印机）上，则可以通过连接纸张输送控制装置来控制这些可选装置来控制这些选项。 这种图像形成装置具有视频控制器，用于将从诸如主计算机的高阶装置发送的打印机语言转换为位图数据和打印机引擎以打印位图数据。 这种与视频控制器电连接的纸张输送控制装置具有与视频控制器共享的存储器。 视频控制器将作业计划写入共享内存。 纸张输送控制装置根据书面作业时间表来控制可选装置。