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1.发明授权Method and composition for quantitative determination of ammonia, .alpha.-amino acid, or .alpha.-keto acid 失效用于定量测定氨，α-氨基酸或α-酮酸的方法和组合物
公开(公告)号：US5780256A
公开(公告)日：1998-07-14
申请号：US108736
申请日：1995-03-13
申请人： Shigeru Ueda , Mamoru Takahashi , Hideo Misaki , Shigeru Ikuta
发明人： Shigeru Ueda , Mamoru Takahashi , Hideo Misaki , Shigeru Ikuta
IPC分类号： C12Q1/32
CPC分类号： C12Q1/32
摘要： The present invention is directed to a method for the quantitative determination of ammonia, an .alpha.-amino acid and an .alpha.-keto acid corresponding to the .alpha.-amino acid, or a chemical substance producing any one of these compounds. The present invention is also directed to an analytical composition for use in the above method. The method of the present invention ensures rapidness and accuracy in the determination of ammonia, .alpha.-amino acids or .alpha.-keto acids, even with the use of a small quantity of a biological sample. This method is very useful in application fields, such as clinical diagnosis and food testing.
摘要翻译： PCT No.PCT / JP91 / 01785 Sec。 371日期1995年3月13日 102（e）1995年3月13日PCT PCT 1991年12月27日PCT公布。 WO92 / 15705 PCT公开号 日期1992年9月17日本发明涉及一种定量测定对应于α-氨基酸的氨，α-氨基酸和α-酮酸的方法，或者是产生这些化合物中的任何一种的化学物质。 本发明还涉及用于上述方法的分析组合物。 本发明的方法即使使用少量的生物样品也能确保氨，α-氨基酸或α-酮酸的测定的快速和准确性。 该方法在临床诊断和食品检测等应用领域非常有用。
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2.发明授权
公开(公告)号：US5633143A
公开(公告)日：1997-05-27
申请号：US244450
申请日：1994-05-26
申请人： Shigeru Ueda , Hideo Misaki , Shigeru Ikuta , Mamoru Takahashi
发明人： Shigeru Ueda , Hideo Misaki , Shigeru Ikuta , Mamoru Takahashi
CPC分类号： C12Q1/32 , Y10S435/966 , Y10S435/973 , Y10T436/104998
摘要： Disclosed is a method for the quantitative determination of D-3-hydroxybutyric acid and acetoacetic acid, which comprises reacting a biological sample containing D-3-hydroxybutyric acid and acetoacetic acid, with a reagent comprising: (1) a D-3-hydroxybutyrate dehydrogenase, (2) A.sub.1 and (3) B.sub.1, the components (1), (2) and (3) participating in the following cycling reaction: ##STR1## thereby effecting the enzymatic cycling reaction, and measuring a change in the amount of A.sub.2 formed or the amount of B.sub.1 consumed. Also disclosed is an analytical reagent comprising the components (1), (2) and (3) for use in the above method. The method and the analytical reagent ensure rapidness and accuracy in the determination of D-3-hydroxybutyric acid and acetoacetic acid, even with the use of a small quantity of a biological sample, so that they are very useful in application fields, such as clinical diagnosis and food testing.
摘要翻译： PCT No.PCT / JP91 / 01706 Sec。 371日期：1994年5月26日 102（e）日期1994年5月26日PCT 1991年12月12日PCT PCT。 公开号WO93 / 12254 日期：1993年6月24日公开是定量测定D-3-羟基丁酸和乙酰乙酸的方法，其包括使含有D-3-羟基丁酸和乙酰乙酸的生物样品与包含以下物质的试剂反应：（1） D-3-羟基丁酸脱氢酶，（2）A1和（3）B1，参与以下循环反应的组分（1），（2）和（3）：进行酶循环反应，并测量 形成的A2的量的变化或消耗的B1的量。 还公开了包含用于上述方法的组分（1），（2）和（3）的分析试剂。 该方法和分析试剂确保即使使用少量生物样品也能快速，准确地测定D-3-羟基丁酸和乙酰乙酸，因此它们在临床应用领域非常有用 诊断和食品检测。
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3.发明授权Highly sensitive assay method for myo-inositol, composition for practicing same, novel myo-inositol dehydrogenase, and process for producing same 失效肌醇的高灵敏度测定方法，实践相同的组合物，新型肌醇脱氢酶及其制备方法
公开(公告)号：US5356790A
公开(公告)日：1994-10-18
申请号：US106693
申请日：1993-08-16
CPC分类号： C12N9/0006 , C12Q1/02 , C12Q1/32 , C12R1/07 , Y10S436/817
摘要： Myo-inositol in a specimen is assayed by reacting a specimen containing myo-inositol with:a) myo-inositol dehydrogenase using a thio-NADP group or thio-NAD group and an NADP group or NAD group as coenzymes, and which catalyzes a reversible reaction forming myo-inosose from myo-inositol,b ) A.sub.1 andc) B.sub.1to effect a cycling reaction ##STR1## wherein A.sub.1 is a thio-NADP group, thio-NAD group, NADP group or NAD group, A.sub.2 is a reduced form of A.sub.1, when A.sub.1 is a thio-NADP group or thio-NAD group, B.sub.1 is a reduced NADP group or reduced NAD group and when A.sub.1 is an NADP group or NAD group, B.sub.1 is a reduced thio-NADP group or reduced thio-NAD group, and wherein B.sub.2 is an oxidized form of B.sub.1. The change in the amount of A.sub.2 generated or B.sub.1 consumed by the cycling reaction is measured to perform the assay. A composition for performing the assay comprises the above myo-inositol dehydrogenase, as well as the above components A.sub.1 and B.sub.1. The myo-inositol dehydrogenase can be produced by culturing a suitable microorganism belonging to genus Bacillus, particularly Bacillus sp. No. 3 FERM BP-3013.
摘要翻译： 通过使含有肌醇的样品与以下物质反应来测定样品中的肌醇：a）使用硫代NADP基团或硫代NAD基团和NADP基团或NAD基团作为辅酶的肌醇脱氢酶，并催化可逆的 由肌球蛋白形成肌内单糖的反应，b）A1和c）B1进行循环反应，其中A1是硫代NADP基团，硫代NAD基团，NADP基团或NAD基团，A2是还原形式 的A1，当A1是硫代NADP基团或硫代NAD基团时，B1是还原的NADP基团或还原的NAD基团，当A1是NADP基团或NAD基团时，B1是还原的硫代NADP基团或还原的硫代 - NAD基，其中B2是B1的氧化形式。 测量由循环反应消耗的A2产生量或B1的量的变化以进行测定。 用于进行测定的组合物包含上述肌醇脱氢酶，以及上述组分A1和B1。 肌醇脱氢酶可以通过培养属于芽孢杆菌属（Bacillus），特别是芽孢杆菌属（Bacillus sp。）的合适的微生物来产生。 3号FERM BP-3013。
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公开(公告)号：US5126246A
公开(公告)日：1992-06-30
申请号：US435003
申请日：1989-11-09
CPC分类号： C12Q1/48 , C12Q1/44 , Y10S435/81 , Y10S435/832 , Y10S436/815 , Y10S530/825 , Y10S530/83
摘要： Reagent for analysis of triglycerides contained in blood serum is provided, which comprises lipases and monoglyceride lipases capable of acting on monoglycerides having substrate specificity and capable of catalyzing the following enzymatic reaction: monoglyceride+H.sub.2 O.fwdarw.glycerol+fatty acids. The glycerol or fatty acids are measured to learn an amount of the triglycerides or fatty acid by any known analytical method.
摘要翻译： 提供了用于分析血清中含有的甘油三酯的试剂，其包含能够作用于具有底物特异性并能够催化以下酶促反应的单酸甘油酯的脂肪酶和甘油单酯脂肪酶：单酸甘油酯+ H 2 O->甘油+脂肪酸。 测量甘油或脂肪酸，以通过任何已知的分析方法学习一定量的甘油三酯或脂肪酸。
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5.发明授权Novel NAD synthetase, assay method using said novel NAD synthetase and a process for production thereof 失效新型NAD合成酶，使用所述新型NAD合成酶的测定方法及其生产方法
公开(公告)号：US4921786A
公开(公告)日：1990-05-01
申请号：US94813
申请日：1987-09-10
摘要： A novel NAD synthetase is produced by culturing a broth of Bacillus stearothermophilus H-804 FERM BP-1408. This new enzyme selectively catalyzes the reaction ##STR1## without catalyzing the reaction ##STR2## The enzyme uses ammonia or ammonium ion as a substrate, but does not use either glutamine or asparagine. Also disclosed is an assay method using the enzyme, for any one of ATP, deamide-NAD, ammonia or ammonium ion in a specimen to be assayed.
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公开(公告)号：US5079158A
公开(公告)日：1992-01-07
申请号：US171272
申请日：1988-03-21
CPC分类号： C12N9/18 , C12Q1/44 , Y10S435/832
摘要： Disclosed herein is a novel monoglyceride lipase at least capable of catalyzing an enzymatic reaction of the following equation (a) and as substrate specificity, capable of acting on monoglyceride but incapable of acting on diglyceride and triglyceride:(a) Monoglyceride+H.sub.2 O.fwdarw.Glycerol+Fatty acidThe monoglyceride lipase is produced by culturing a specific monoglyceride lipase producing microorganism of Bacillus and then collecting the monoglyceride lipase from the resulting culture. A method is also disclosed for the analysis of a monoglyceride-containing sample solution. The monoglyceride lipase is caused to act on the sample solution upon measurement of the monoglyceride in the sample solution. Either one of glycerol and the fatty acid formed in accordance with the equation (a) is then measured.
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7.发明授权Process for the production of L-alanine dehydrogenase from 78-3 FERM BP-2517 失效从78-3 FERM BP-2517生产L-苯胺脱氢酶的方法
公开(公告)号：US5116748A
公开(公告)日：1992-05-26
申请号：US413431
申请日：1989-09-26
CPC分类号： C12N9/0016 , C12R1/01
摘要： A process for the production of L-alanine dehydrogenase which comprises culturing an L-alanine dehydrogenase producing microorganism belonging to the genus Sporolactobacillus in a nutrient medium and isolating thus-produced L-alanine dehydrogenase from the cultured mass. The microorganism is for example Sporolactobacillus sp. 78-3 FERM BP-2517 and mutants and variants thereof having the ability to produce L-alanine dehydrogenase in recoverable amounts. This strain is a thermophile which cannot grow at 40.degree. C. but can grow at 45.degree. C. and 52.degree. C. The strain can assimilate glucose and produce lactic acid and acetic acid.
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8.发明授权Highly sensitive assay method for L-carnitine and composition for practicing same 失效L-肉碱的高灵敏度测定方法及其用途
公开(公告)号：US5266463A
公开(公告)日：1993-11-30
申请号：US640117
申请日：1991-01-11
申请人： Mamoru Takahashi , Shigeru Ueda
发明人： Mamoru Takahashi , Shigeru Ueda
CPC分类号： C12N9/0006 , C12Q1/26 , C12Q1/32 , Y10S435/829 , Y10S436/815
摘要： A method of assaying L-carnitine in a specimen comprises reacting a specimen containing L-carnitine with:a) L-carnitine dehydrogenase having coenzymes of the thio-NAD group and of the NAD group, and which catalyzes a reversible reaction forming dehydrocarnitine from a substrate of carnitine,b) A.sub.1 andc) B.sub.1to effect a cycling reaction of the formula ##STR1## wherein A.sub.1 is thio-NAD group or NAD group, A.sub.2 is a reduced form of A.sub.1, when A.sub.1 is thio-NAD group, B.sub.1 is reduced NAD group and when A.sub.1 is NAD group, B.sub.1 is reduced thio-NAD, and wherein B.sub.2 is an oxidized form of B.sub.1 ; and measuring an amount of A.sub.2 or B.sub.1 generated or consumed by the cycling reaction. A composition for performing the assay comprises the above L-carnitine dehydrogenase, as well as the above components A.sub.1 and B.sub.1.
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9.发明授权
公开(公告)号：US5385829A
公开(公告)日：1995-01-31
申请号：US774221
申请日：1991-10-09
申请人： Mamoru Takahashi , Shigeru Ueda
发明人： Mamoru Takahashi , Shigeru Ueda
CPC分类号： C12P13/007 , C12N9/18 , C12P41/004 , C12Q1/44 , C12R1/05 , G01N2333/904 , G01N2333/906 , Y10S435/829 , Y10S436/815
摘要： A method of assaying for acyl-L-carnitines including short chain acyl-carnitines including acetyl-L-carnitine and propionyl-L-carnitine in a substance, comprises subjecting a sample of the substance to be analyzed to an enzymatic hydrolysis using an acyl-carnitine esterase. The esterase is produced by Alcaligenes sp. FERM BP-2570 and it has substrate specificity for acyl-L-carnitines including short-chain acyl-L-carnitines. In addition the esterase demonstrates substrate specificity for acetyl-L-carnitine and propionyl-L-carnitine. The enzyme facilitates the hydrolysis reaction of one mole each of the acyl-L-carnitines with one mole of water in which to form one mole each of the corresponding fatty acid and L-carnitine. The amount of the fatty acid and L-carnitine formed is determined by this method.
摘要翻译： 一种在物质中测定包括乙酰基-L-肉碱和丙酰基-L-肉碱的短链酰基肉碱的酰基-L-肉碱的方法包括：使用酰基-L-肉毒碱将待分析物质的样品进行酶水解， 肉碱酯酶。 酯酶由产碱杆菌属产生。 FERM BP-2570，它具有酰基-L-肉碱（包括短链酰基-L-肉碱）的底物特异性。 此外，酯酶显示乙酰基-L-肉碱和丙酰基-L-肉碱的底物特异性。 该酶促进1摩尔酰基-L-肉碱与1摩尔水的水解反应，其中形成1摩尔相应的脂肪酸和L-肉毒碱。 通过该方法测定形成的脂肪酸和L-肉毒碱的量。
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公开(公告)号：US20100276325A1
公开(公告)日：2010-11-04
申请号：US10549179
申请日：2004-03-18
CPC分类号： B65D79/02 , C12Q1/26 , G01N2333/90222 , G01N2333/90235
摘要： An oxygen indicator using an optical absorption spectral change reaction caused by a substrate in the presence of oxygen via an enzymatic catalysis, which comprises an oxygen sensitive solution containing at least a coloring substrate, an oxidoreductase, and a reducing agent capable of reducing the oxidized coloring substrate.
摘要翻译： 一种氧指示剂，其通过酶催化在氧气存在下由底物引起的光吸收光谱变化反应，其包含至少含有着色底物的氧敏感性溶液，氧化还原酶和能够还原氧化着色的还原剂 基质。
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