Method for analyzing cell, chip for cell analysis, reagent for cell analysis, kit for cell analysis, and apparatus for cell analysis

    公开(公告)号:US11199541B2

    公开(公告)日:2021-12-14

    申请号:US16065947

    申请日:2016-08-04

    Abstract: According to some aspects, a method for analyzing a cell is provided. The method includes trapping the cell by binding a first molecule to the cell. The method further includes binding a second molecule to the cell. The second molecule includes a binding portion capable of specific binding to a cell-surface molecule of the cell. The second molecule further includes an identifying portion, a labeling portion coupled to the identifying portion, and a stimulus-degradable linker between the binding portion and the identification portion. The method further includes detaching the identifying portion from the binding portion by stimulating the stimulus-degradable linker where the detached identifying portion is coupled to the labeling portion. The method further includes binding the detached identifying portion through specific binding to an identifying portion recognizing molecule and detecting the labeling portion.

    METHOD FOR ANALYZING CELL, CHIP FOR CELL ANALYSIS, REAGENT FOR CELL ANALYSIS, KIT FOR CELL ANALYSIS, AND APPARATUS FOR CELL ANALYSIS

    公开(公告)号:US20180372741A1

    公开(公告)日:2018-12-27

    申请号:US16065947

    申请日:2016-08-04

    Abstract: According to some aspects, a method for analyzing a cell is provided. The method includes trapping the cell by binding a first molecule to the cell. The method further includes binding a second molecule to the cell. The second molecule includes a binding portion capable of specific binding to a cell-surface molecule of the cell. The second molecule further includes an identifying portion, a labeling portion coupled to the identifying portion, and a stimulus-degradable linker between the binding portion and the identification portion. The method further includes detaching the identifying portion from the binding portion by stimulating the stimulus-degradable linker where the detached identifying portion is coupled to the labeling portion. The method further includes binding the detached identifying portion through specific binding to an identifying portion recognizing molecule and detecting the labeling portion.

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