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公开(公告)号：US20230158502A1
公开(公告)日：2023-05-25
申请号：US17919398
申请日：2020-04-17
Applicant: SPHERE FLUIDICS LIMITED
Inventor： Dimitris Josephides , James LONG , Xin LI
IPC: B01L3/00
CPC classification number: B01L3/502784 , B01L2200/0636 , B01L2400/0487 , B01L2400/086 , B01L2300/0867
Abstract: We describe a microfluidic structure for spacing out droplets, the structure comprising: a main channel for guiding droplets in a spacing fluid; a first inlet for introducing droplets into the main channel; and a second inlet for introducing a spacing fluid into the main channel, wherein a cross-sectional area of the main channel decreases downstream from the first inlet. We also describe a method of spacing out droplets using a microfluidic structure.
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公开(公告)号：US12190572B2
公开(公告)日：2025-01-07
申请号：US17266447
申请日：2019-08-06
Applicant: Sphere Fluidics Limited
Inventor： Nicholas Dayrell-Armes , David Holmes , Frank F Craig , Marian Rehak , Dimitris Josephides , Robert Salter , William Whitley , Sinan Gokkaya , Raphael Ruis
IPC: G06V10/82 , B01L3/00 , G01N15/14 , G01N15/1433 , G06F18/2431 , G06T7/70 , G06V10/44 , G06V10/764 , G06V20/69 , G01N15/10
Abstract: A method of processing droplets in a microfluidic system. The method may comprise capturing a time sequence of images of a droplet as it passes through a channel in a microfluidic system. The method may further comprise processing each image of the sequence of images using a convolutional neural network to count a number of cells or other entities visible in each image the droplet. The method may further comprise processing the count of the number of cells or other entities visible in each image of the droplet to determine an estimated number of cells or other entities in the droplet. The method/system may further comprise controlling a microfluidic process performed on the droplet responsive to the estimated number of cells or other entities in the droplet. Implementations of the method use the changing orientation and disposition of droplet contents in combination with machine learning to improve monoclonality assurance.
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公开(公告)号：US11919001B2
公开(公告)日：2024-03-05
申请号：US16625021
申请日：2018-06-21
Applicant: SPHERE FLUIDICS LIMITED
Inventor： Dimitris Josephides , Marian Rehak , David Holmes , Frank F. Craig
IPC: B01L3/00
CPC classification number: B01L3/502784 , B01L3/502761 , B01L3/502715 , B01L2200/027 , B01L2200/0652 , B01L2200/0673 , B01L2400/0487
Abstract: This invention generally relates to methods and systems for dispensing a droplet, in particular a picolitre volume droplet containing one or more entities. We disclose herein a microfluidic droplet dispensing system comprising: a droplet feed line to carry droplets for dispensing; a dispensing line to carry dispensing fluid for dispensing the droplets; a droplet outlet line coupled to a droplet outlet; a first junction having a first input to receive a flow from said droplet feed line, having a second input to receive a flow from said dispensing line and having an output to said droplet outlet line; a waste line coupled to said droplet output line between said first junction and said droplet output; a first pressure source to provide pressurised dispensing fluid to said dispensing line to eject droplets from said droplet output; a droplet dispensing valve between said first pressure source and said dispensing line to control the ejection of droplets from said droplet output; wherein the system further comprises: a second pressure source to provide pressurised dispensing fluid to a droplet protect line; a second junction having a first input from said droplet feed line, having a second input from said droplet protect line, and having an output; and a volume flow throttle coupled between the output of said second junction and the first input of said first junction to restrict a volume flow from said second junction into said first junction.
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公开(公告)号：US20210293691A1
公开(公告)日：2021-09-23
申请号：US17266447
申请日：2019-08-06
Applicant: Sphere Fluidics Limited
Inventor： Nicholas Dayrell-Armes , David Holmes , Frank F Craig , Marian Rehak , Dimitris Josephides , Robert Salter , William Whitley , Sinan Gokkaya , Raphael Ruis
Abstract: A method of processing droplets in a microfluidic system. The method may comprise capturing a time sequence of images of a droplet as it passes through a channel in a microfluidic system. The method may further comprise processing each image of the sequence of images using a convolutional neural network to count a number of cells or other entities visible in each image the droplet. The method may further comprise processing the count of the number of cells or other entities visible in each image of the droplet to determine an estimated number of cells or other entities in the droplet. The method/system may further comprise controlling a microfluidic process performed on the droplet responsive to the estimated number of cells or other entities in the droplet. Implementations of the method use the changing orientation and disposition of droplet contents in combination with machine learning to improve monoclonality assurance.
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