公开(公告)号：US20170234893A1

公开(公告)日：2017-08-17

申请号：US15269502

申请日：2016-09-19

申请人： Sten Ohlson ,  Anthony R. Torres

发明人： Sten Ohlson ,  Anthony R. Torres

IPC分类号： G01N33/74 ,  B01D15/38 ,  B01D15/22 ,  G01N30/72

CPC分类号： G01N33/743 ,  B01D15/22 ,  B01D15/3804 ,  G01N30/7233 ,  G01N2030/8813 ,  G01N2430/00 ,  G01N2560/00

摘要： The present invention provides methods for analyzing a target compound from a biological sample. In one aspect, a method for analyzing a target compound in a biological sample can comprise delivering a biological sample through an affinity column, the affinity column having a binding ligand coupled to a stationary structural support, wherein the affinity column has a high density of the binding ligand per the stationary structural support and wherein the binding ligand has been preselected to cause weak affinity separation zonal retardation of the target compound from the biological sample forming a target compound fraction and a biological sample fraction and detecting the target compound by mass spectrometry.

公开(公告)号：US09448243B2

公开(公告)日：2016-09-20

申请号：US14477695

申请日：2014-09-04

申请人： Sten Ohlson ,  Anthony R. Torres

发明人： Sten Ohlson ,  Anthony R. Torres

IPC分类号： G01N33/74 ,  B01D15/38 ,  G01N30/72 ,  G01N30/88

CPC分类号： G01N33/743 ,  B01D15/22 ,  B01D15/3804 ,  G01N30/7233 ,  G01N2030/8813 ,  G01N2430/00 ,  G01N2560/00

摘要： The present invention provides methods for analyzing a target compound from a biological sample. In one aspect, a method for analyzing a target compound in a biological sample can comprise delivering a biological sample through an affinity column, the affinity column having a binding ligand coupled to a stationary structural support, wherein the affinity column has a high density of the binding ligand per the stationary structural support and wherein the binding ligand has been preselected to cause weak affinity separation zonal retardation of the target compound from the biological sample forming a target compound fraction and a biological sample fraction and detecting the target compound by mass spectrometry.

摘要翻译： 本发明提供了从生物样品中分析目标化合物的方法。 一方面，用于分析生物样品中的目标化合物的方法可以包括通过亲和柱输送生物样品，亲和柱具有与固定结构载体偶联的结合配体，其中亲和柱具有高密度的 结合配体，其中结合配体已被预先选择以产生目标化合物的弱亲和力分离，来自形成目标化合物部分和生物样品部分的生物样品的区域延迟，并通过质谱法检测目标化合物。

公开(公告)号：US20150125877A1

公开(公告)日：2015-05-07

申请号：US14477695

申请日：2014-09-04

申请人： Sten Ohlson ,  Anthony R. Torres

发明人： Sten Ohlson ,  Anthony R. Torres

IPC分类号： G01N33/74

CPC分类号： G01N33/743 ,  B01D15/22 ,  B01D15/3804 ,  G01N30/7233 ,  G01N2030/8813 ,  G01N2430/00 ,  G01N2560/00

摘要： The present invention provides methods for analyzing a target compound from a biological sample. In one aspect, a method for analyzing a target compound in a biological sample can comprise delivering a biological sample through an affinity column, the affinity column having a binding ligand coupled to a stationary structural support, wherein the affinity column has a high density of the binding ligand per the stationary structural support and wherein the binding ligand has been preselected to cause weak affinity separation zonal retardation of the target compound from the biological sample forming a target compound fraction and a biological sample fraction and detecting the target compound by mass spectrometry.

摘要翻译： 本发明提供从生物样品中分析目标化合物的方法。 在一个方面，用于分析生物样品中的目标化合物的方法可以包括通过亲和柱递送生物样品，亲和柱具有与固定结构载体偶联的结合配体，其中亲和柱具有高密度的 结合配体，并且其中结合配体已被预先选择以产生目标化合物的弱亲和力分离，来自形成靶化合物部分和生物样品部分的生物样品的区域延迟，并通过质谱法检测目标化合物。

公开(公告)号：US20110039260A1

公开(公告)日：2011-02-17

申请号：US12745130

申请日：2008-11-26

申请人： Robert D. Jenison ,  Joshua Klonoski ,  Anthony R. Torres

发明人： Robert D. Jenison ,  Joshua Klonoski ,  Anthony R. Torres

IPC分类号： C12Q1/68 ,  G01N33/53

CPC分类号： C12Q1/682 ,  C12Q1/6804 ,  G01N33/54306 ,  Y10T436/143333 ,  C12Q2527/137 ,  C12Q2527/125

摘要： Methods and materials are disclosed relating to an improved method for amplifying a signal in a diagnostic assay for a nucleic acid, comprising the steps of providing an amplification polymer bound to a nucleic acid analyte, wherein the amplification polymer comprises a plurality of amine groups; binding amine groups on the amplification polymer with a detectable label complex; and reacting under high salt conditions an acetylating compound with amine groups not bound with a detectable label complex.

摘要翻译： 公开了关于用于扩增核酸诊断测定中的信号的改进方法的方法和材料，其包括提供与核酸分析物结合的扩增聚合物的步骤，其中所述扩增聚合物包含多个胺基团; 在扩增聚合物上具有可检测标记复合物的结合胺基; 并在高盐条件下使乙酰化化合物与未被可检测标记物复合物结合的胺基反应。

公开(公告)号：US20130164827A1

公开(公告)日：2013-06-27

申请号：US13669199

申请日：2012-11-05

申请人： Anthony R. Torres

发明人： Anthony R. Torres

IPC分类号： C07K1/18 ,  C07K1/36

CPC分类号： C07K1/18 ,  C07K1/36

摘要： The present invention provides methods for separating proteins from a protein mixture. In one aspect, a method for separating a high concentration protein mixture into a bound protein fraction and a flow-through protein fraction can include delivering a protein mixture through an ion exchange column at a fixed pH and a fixed salt concentration. The fixed pH and the fixed salt concentration have been preselected to cause separation of the protein mixture into a bound protein fraction and a flow-through protein fraction. In this case, the bound protein fraction binds to the ion exchange column and the flow-though protein fraction flows though the ion exchange column. The method can further include receiving the flow-through protein fraction from the ion exchange column separate from the bound protein fraction, wherein either the bound protein fraction or the flow-through fraction contains a protein of interest.

摘要翻译： 本发明提供了从蛋白质混合物中分离蛋白质的方法。 一方面，将高浓度蛋白质混合物分离成结合蛋白质级分和流通蛋白质级分的方法可以包括以固定的pH和固定的盐浓度将蛋白质混合物输送通过离子交换柱。 已经预选了固定的pH和固定盐浓度，以使蛋白质混合物分离成结合的蛋白质级分和流通蛋白质级分。 在这种情况下，结合的蛋白质级分与离子交换柱结合，流过蛋白质级分流过离子交换柱。 该方法还可以包括从结合的蛋白质级分分离出离子交换柱的流通蛋白质级分，其中结合的蛋白质级分或流通级分含有目的蛋白质。

公开(公告)号：US20090221096A1

公开(公告)日：2009-09-03

申请号：US12096817

申请日：2006-12-13

申请人： Anthony R. Torres

发明人： Anthony R. Torres

IPC分类号： G01N33/53 ,  G01N21/00 ,  B05D1/36 ,  G01N33/543

CPC分类号： G01N33/54373 ,  G01N33/54393

摘要： Thin-film biosensor chips for detecting a target analyte in a biological sample are disclosed. The chips include a solid substrate, an antireflective optical layer, an attachment layer using a non-polymeric silane, and an Fc-specific binding molecule coupled to the non-polymeric silane. Kits containing the chips and methods of using and making the chips are also disclosed.

摘要翻译： 公开了用于检测生物样品中的目标分析物的薄膜生物传感器芯片。 芯片包括固体衬底，抗反射光学层，使用非聚合硅烷的附着层和与非聚合硅烷偶联的Fc特异性结合分子。 还公开了包含芯片的套件和使用和制造芯片的方法。

公开(公告)号：US08993334B2

公开(公告)日：2015-03-31

申请号：US13669199

申请日：2012-11-05

申请人： Anthony R. Torres

发明人： Anthony R. Torres

IPC分类号： C07K1/36 ,  C07K1/18 ,  C07K1/00

CPC分类号： C07K1/18 ,  C07K1/36

摘要： The present invention provides methods for separating proteins from a protein mixture. In one aspect, a method for separating a high concentration protein mixture into a bound protein fraction and a flow-through protein fraction can include delivering a protein mixture through an ion exchange column at a fixed pH and a fixed salt concentration. The fixed pH and the fixed salt concentration have been preselected to cause separation of the protein mixture into a bound protein fraction and a flow-through protein fraction. In this case, the bound protein fraction binds to the ion exchange column and the flow-though protein fraction flows though the ion exchange column. The method can further include receiving the flow-through protein fraction from the ion exchange column separate from the bound protein fraction, wherein either the bound protein fraction or the flow-through fraction contains a protein of interest.

摘要翻译： 本发明提供了从蛋白质混合物中分离蛋白质的方法。 一方面，将高浓度蛋白质混合物分离成结合蛋白质级分和流通蛋白质级分的方法可以包括在固定的pH和固定的盐浓度下将蛋白质混合物输送通过离子交换柱。 已经预选了固定的pH和固定盐浓度，以使蛋白质混合物分离成结合的蛋白质级分和流通蛋白质级分。 在这种情况下，结合的蛋白质级分与离子交换柱结合，流过蛋白质级分流过离子交换柱。 该方法还可以包括从结合的蛋白质级分分离出离子交换柱的流通蛋白质级分，其中结合的蛋白质级分或流通级分含有目的蛋白质。

公开(公告)号：US06555661B1

公开(公告)日：2003-04-29

申请号：US09486417

申请日：2000-02-25

申请人： Anthony R. Torres ,  Walter H. Runkis

发明人： Anthony R. Torres ,  Walter H. Runkis

IPC分类号： C07K114

CPC分类号： C07K1/18 ,  C07K14/31

摘要： High purity protein A is recovered from protein A preparations by passing a protein A preparation over an ion exchange column so that protein A will bind to a cation exchange resin. The loaded column is washed with a wash buffer solution having about the same pH as the protein A preparation and an increased mineral salt concentration to elute certain impurities but not the protein A. The protein A is eluted from the washed column by eluting the column with an elution buffer solution having a pH higher than the wash buffer solution and mineral salt concentration substantially no higher than the wash buffer solution. The protein A can be easily recovered from protein A preparations that do not use any human or animal components. The protein A purified by the invention is suitable for all common uses of protein A, but is especially well-suited for human therapeutic applications.

摘要翻译： 通过将蛋白A制剂通过离子交换柱从蛋白A制备中回收高纯度蛋白A，使蛋白A与阳离子交换树脂结合。 负载的柱用与蛋白A制备具有相同pH的洗涤缓冲溶液洗涤，并且增加的矿物质盐浓度以洗脱某些杂质而不是蛋白质A.蛋白质A通过用洗脱柱洗脱， 洗脱缓冲溶液，其pH高于洗涤缓冲溶液，矿物盐浓度基本上不高于洗涤缓冲溶液。 蛋白A可以从不使用任何人或动物成分的蛋白A制剂中容易地回收。 本发明纯化的蛋白A适用于蛋白A的所有常见用途，但特别适用于人类治疗应用。

公开(公告)号：US08574833B2

公开(公告)日：2013-11-05

申请号：US12745130

申请日：2008-11-26

申请人： Robert D. Jenison ,  Joshua Klonoski ,  Anthony R. Torres

发明人： Robert D. Jenison ,  Joshua Klonoski ,  Anthony R. Torres

IPC分类号： C12Q1/68 ,  C07H21/00 ,  B01L3/00

CPC分类号： C12Q1/682 ,  C12Q1/6804 ,  G01N33/54306 ,  Y10T436/143333 ,  C12Q2527/137 ,  C12Q2527/125

摘要： Methods and materials are disclosed relating to an improved method for amplifying a signal in a diagnostic assay for a nucleic acid, comprising the steps of providing an amplification polymer bound to a nucleic acid analyte, wherein the amplification polymer comprises a plurality of amine groups; binding amine groups on the amplification polymer with a detectable label complex; and reacting under high salt conditions an acetylating compound with amine groups not bound with a detectable label complex.

公开(公告)号：US20110118453A1

公开(公告)日：2011-05-19

申请号：US12619421

申请日：2009-11-16

申请人： Anthony R. Torres

发明人： Anthony R. Torres

IPC分类号： C07K1/18

CPC分类号： C07K1/18 ,  C07K1/36

摘要： The present invention provides methods for separating proteins from a protein mixture. In one aspect, a method for separating a high concentration protein mixture into a bound protein fraction and a flow-through protein fraction can include delivering a protein mixture through an ion exchange column at a fixed pH and a fixed salt concentration. The fixed pH and the fixed salt concentration have been preselected to cause separation of the protein mixture into a bound protein fraction and a flow-through protein fraction. In this case, the bound protein fraction binds to the ion exchange column and the flow-though protein fraction flows though the ion exchange column. The method can further include receiving the flow-through protein fraction from the ion exchange column separate from the bound protein fraction, wherein either the bound protein fraction or the flow-through fraction contains a protein of interest.

摘要翻译： 本发明提供了从蛋白质混合物中分离蛋白质的方法。 一方面，将高浓度蛋白质混合物分离成结合蛋白质级分和流通蛋白质级分的方法可以包括在固定的pH和固定的盐浓度下将蛋白质混合物输送通过离子交换柱。 已经预选了固定的pH和固定盐浓度，以使蛋白质混合物分离成结合的蛋白质级分和流通蛋白质级分。 在这种情况下，结合的蛋白质级分与离子交换柱结合，流过蛋白质级分流过离子交换柱。 该方法还可以包括从结合的蛋白质级分分离出离子交换柱的流通蛋白质级分，其中结合的蛋白质级分或流通级分含有目的蛋白质。