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    DEVICES AND METHODS FOR DETECTING AND QUANTITATING NUCLEIC ACIDS USING SIZE SEPARATION OF AMPLICONS
    6.
    发明申请
    DEVICES AND METHODS FOR DETECTING AND QUANTITATING NUCLEIC ACIDS USING SIZE SEPARATION OF AMPLICONS 审中-公开
    使用大小分离核酸检测和定量核酸的装置和方法

    公开(公告)号：US20090186344A1

    公开(公告)日：2009-07-23

    申请号：US12056213

    申请日：2008-03-26

    申请人： Javier A. Farinas

    发明人： Javier A. Farinas

    IPC分类号： C12Q1/68

    CPC分类号： B01L3/5027 ,  B01L3/502715 ,  B01L3/5082 ,  B01L7/52 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/141 ,  B01L2300/0816 ,  B01L2400/0421 ,  B01L2400/0487 ,  G01N27/44756

    摘要： Devices and methods are described for detecting and quantifying nucleic acids using a sealed system that minimizes contamination. In particular, provided herein are devices for and methods using nucleic acid amplification that permit multiple sampling of an amplification reaction mixture and quantitation and identification of amplicons during the course of an amplification reaction. Methods involving the transfer of samples from an amplification reaction mixture into a separation network, separation of nucleic acids based on size, and identification and quantitation of nucleic acids are disclosed.

    摘要翻译： 描述了使用最小化污染的密封系统来检测和定量核酸的装置和方法。 特别地，本文提供了使用核酸扩增的装置和方法，其允许扩增反应混合物的多次取样以及扩增反应过程中扩增子的定量和鉴定。 涉及将样品从扩增反应混合物转移到分离网络中的方法，基于大小分离核酸以及核酸的鉴定和定量。

    Optical detection of transmembrane potential changes
    7.
    发明授权
    Optical detection of transmembrane potential changes 失效
    光学检测跨膜电位变化

    公开(公告)号：US06468763B1

    公开(公告)日：2002-10-22

    申请号：US09661337

    申请日：2000-09-14

    申请人： Javier A. Farinas

    发明人： Javier A. Farinas

    IPC分类号： C12Q102

    CPC分类号： G01N33/58 ,  G01N33/5005 ,  G01N33/536 ,  G01N33/542 ,  G01N33/582 ,  Y10S435/968

    摘要： Compositions for monitoring transmembrane potential across cellular membranes. The compositions typically comprise a cell having a plasma membrane that comprises a first leaflet and a second leaflet, the membrane comprising first and second membrane associated components which, when placed adjacent each other either produce or quench a fluorescent signal, wherein. The first membrane associated component translocates from a first leaflet of the membrane to a second leaflet of the membrane in response to an electrical potential gradient across the membrane, the first membrane associated component being selected from a non-fluorescent cationic fluorescence quencher a non-fluorescent anionic fluorescence quencher and a cationic fluorophore.

    摘要翻译： 用于监测细胞膜跨膜电位的组合物。 组合物通常包含具有质膜的细胞，其包含第一小叶和第二叶，所述膜包含第一和第二膜相关组分，当彼此相邻放置时产生或骤冷荧光信号。 所述第一膜相关组分响应跨过所述膜的电势梯度，从所述膜的第一叶片转移至所述膜的第二叶片，所述第一膜相关组分选自非荧光阳离子荧光猝灭剂，非荧光 阴离子荧光猝灭剂和阳离子荧光团。