摘要:
A process is provided for the preparation of albumin which has extremely low levels of or is essentially free of colorants, metal ions, human proteins, fragments of albumin, polymers or aggregates of albumin, and viruses, and which is relatively non-glycated, relatively high in free thiol and with an intact C-terminus. The process comprises passing albumin (preferably expressed and secreted by transformed yeast) through two chromatography purifications, ultrafiltering the product, passing through two further chromatography steps and again ultrafiltering the product.
摘要:
A process for the preparation of albumin which has extremely low levels of or is essentially free of colorants, metal ions, human proteins, fragments of albumin, polymers or aggregates of albumin, and viruses, and which is relatively non-glycated, relatively high in free thiol and with an intact C-terminus. The process comprises passing albumin (preferably expressed and secreted by transformed yeast) through two chromatography purifications, ultrafiltering the product, passing through two further chromatography steps and again ultrafiltering the product.
摘要:
Problem: when proteins are purified using a protein-binding dye immobilised on a chromatographic matrix, the dye or a portion/derivative may leak into the eluant. Solution: an ion-exchange resin (e.g. Dowex-1) and a disrupting material (e.g. salt and a fatty acid such as sodium octanoate) are used to separate the dye from the protein.
摘要:
When proteins are purified using a protein-binding dye immobilized on a chromatographic matrix, the dye or a portion/derivative may leak into the eluant. An ion-exchange resin (e.g. Dowex-1) and a disrupting material (e.g. salt and a fatty acid such as sodium octanoate) are used to separate the dye from the protein to overcome the protein of the leaking dye.
摘要:
A method of generating anthropomorphic vehicle images for a vehicle owner is shown that includes providing a plurality of anthropomorphic vehicle images on a visual display for a vehicle owner to select; receiving a selection of an anthropomorphic vehicle image from the vehicle owner using a wireless device; obtaining vehicle data using one or more vehicle sensors located on the vehicle; altering at least a portion of the selected anthropomorphic vehicle image based on the accessed vehicle data; and displaying the altered anthropomorphic vehicle image on a wireless device to the vehicle owner.
摘要:
A system and method for updating and communicating traffic information to at least one receiving vehicle includes a traffic information center and at least one probe vehicle. In a preferred embodiment, major road sections are represented as links, the center stores and updates a parent map database of links that are associable with a first value of a condition, each probe vehicle is configured to receive a copy of the database either periodically or upon request, determine a second value of the condition, and compare the first and second values to determine a condition discrepancy. Where the discrepancy surpasses a threshold, each probe vehicle is further configured to upload at least the second value to the center, so as to update the parent map database.
摘要:
The described principles provide a method and system for identifying a potentially closed road based on reroute requests, and of accommodating the potential road closure while continuing to test the road status periodically via routing decisions and rerouting requests. While the optimal route assumes that the potentially closed road is open, a replacement route is defined assuming that the potentially closed road is closed. A fraction of users are sent along the optimal route, and if a reroute request is received, the potentially closed road is assumed to be closed still. The affected user is rerouted to a recovery route that matches the optimal route up to the potentially closed road, and then differs from the optimal route thereafter to avoid the potentially closed road.
摘要:
A method and apparatus for distributing air. The apparatus includes an air distribution vent that distributes air into a compartment, wherein the distribution of air into the compartment causes compartment air from the compartment to be drawn from the compartment. The apparatus also includes a contaminant removal system, wherein compartment air drawn from the compartment is processed to remove contaminants before being distributed back into the compartment by the air distribution vent.
摘要:
A method for providing menu tree assistance includes receiving a voice request from a user via a telematics unit and a wireless network and determining a voice menu tree based on the voice request. The method further includes receiving at least one response based on the voice menu tree, determining a failure or confirmation based on the at least one response and converting the response to data based on the determined confirmation. The method further includes recording the response based on the determined failure and providing the recorded response and data to an advisor at a call center. A system and a computer readable medium including computer program code are also disclosed.
摘要:
A device and method for mounting electrical boxes (1) in preexisting drywall (4) using a mounting bracket (3). To use, a holding wire (14) having a stop (15) on one end is placed through a screw hole (9) of a cover (2) which is attached the electrical box. Then the electrical box and cover are placed sideways through a wall hole (7) in the drywall. Next the electrical box is lowered behind the wall so that conduit (8) may be connected to the electrical box. Then a mounting bracket (3) is secured to the cover and electrical box with holding clips (11) and screws (12) using the holding wire to keep the mounting bracket and cover centered with the wall hole. A standard switch or outlet cover may then be installed to cover the mounting bracket. An additional novel feature of the present invention is a stop strap (17) which may be attached to conduit above a top plate (10) of a wall to bold the conduit in place.