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公开(公告)号：US20090280533A1
公开(公告)日：2009-11-12
申请号：US12463371
申请日：2009-05-08
申请人： Stephen F. Gorfien , Richard M. Fike , Glenn P. Godwin , Joyce L. Dzimian , David A. Epstein , Dale Gruber , Don McClure , Paul J. Price
发明人： Stephen F. Gorfien , Richard M. Fike , Glenn P. Godwin , Joyce L. Dzimian , David A. Epstein , Dale Gruber , Don McClure , Paul J. Price
IPC分类号： C12P21/00
CPC分类号： C12N5/0682 , C07K14/61 , C12N5/0037 , C12N5/0043 , C12N5/005 , C12N5/0603 , C12N7/00 , C12N9/2402 , C12N9/2471 , C12N15/85 , C12N2500/14 , C12N2500/16 , C12N2500/22 , C12N2500/24 , C12N2500/32 , C12N2500/34 , C12N2500/35 , C12N2500/38 , C12N2500/74 , C12N2500/76 , C12N2500/90 , C12N2500/95 , C12N2501/90 , C12N2501/91 , C12N2710/10051 , C12Y302/01023
摘要： The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.
摘要翻译： 本发明提供了支持哺乳动物细胞，特别是上皮细胞和成纤维细胞的体外培养，特别是悬浮液的细胞培养基制剂，以及使用这些培养基在体外悬浮培养哺乳动物细胞的方法。 培养基包含基础培养基和聚阴离子或聚阴离子化合物，优选多磺化或多硫酸化合物，更优选硫酸葡聚糖。 本发明还提供化学上定义的，无蛋白质的真核细胞培养基，其包含铁螯合物和锌，其能够支持悬浮培养物中的生长（特别是哺乳动物细胞的高密度生长），从而增加表达水平 的培养细胞中的重组蛋白，和/或增加培养细胞中的病毒产生。
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公开(公告)号：US08198084B2
公开(公告)日：2012-06-12
申请号：US11151647
申请日：2005-06-14
申请人： Stephen F. Gorfien , Richard M. Fike , Glenn P. Godwin , Joyce L. Dzimian , David A. Epstein , Dale Gruber , Don McClure , Paul J. Price
发明人： Stephen F. Gorfien , Richard M. Fike , Glenn P. Godwin , Joyce L. Dzimian , David A. Epstein , Dale Gruber , Don McClure , Paul J. Price
CPC分类号： C12N5/0682 , C07K14/61 , C12N5/0037 , C12N5/0043 , C12N5/005 , C12N5/0603 , C12N7/00 , C12N9/2402 , C12N9/2471 , C12N15/85 , C12N2500/14 , C12N2500/16 , C12N2500/22 , C12N2500/24 , C12N2500/32 , C12N2500/34 , C12N2500/35 , C12N2500/38 , C12N2500/74 , C12N2500/76 , C12N2500/90 , C12N2500/95 , C12N2501/90 , C12N2501/91 , C12N2710/10051 , C12Y302/01023
摘要： The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.
摘要翻译： 本发明提供了支持哺乳动物细胞，特别是上皮细胞和成纤维细胞的体外培养，特别是悬浮液的细胞培养基制剂，以及使用这些培养基在体外悬浮培养哺乳动物细胞的方法。 培养基包含基础培养基和聚阴离子或聚阴离子化合物，优选多磺化或多硫酸化合物，更优选硫酸葡聚糖。 本发明还提供化学上定义的，无蛋白质的真核细胞培养基，其包含铁螯合物和锌，其能够支持悬浮培养物中的生长（特别是哺乳动物细胞的高密度生长），从而增加表达水平 的培养细胞中的重组蛋白，和/或增加培养细胞中的病毒产生。
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公开(公告)号：US20130065275A1
公开(公告)日：2013-03-14
申请号：US13608803
申请日：2012-09-10
申请人： Stephen F. GORFIEN , Richard M. Fike , Glenn P. Godwin , Joyce L. Dzimian , David A. Epstein , Dale Gruber , Don McClure , Paul J. Price
发明人： Stephen F. GORFIEN , Richard M. Fike , Glenn P. Godwin , Joyce L. Dzimian , David A. Epstein , Dale Gruber , Don McClure , Paul J. Price
CPC分类号： C12N5/0682 , C07K14/61 , C12N5/0037 , C12N5/0043 , C12N5/005 , C12N5/0603 , C12N7/00 , C12N9/2402 , C12N9/2471 , C12N15/85 , C12N2500/14 , C12N2500/16 , C12N2500/22 , C12N2500/24 , C12N2500/32 , C12N2500/34 , C12N2500/35 , C12N2500/38 , C12N2500/74 , C12N2500/76 , C12N2500/90 , C12N2500/95 , C12N2501/90 , C12N2501/91 , C12N2710/10051 , C12Y302/01023
摘要： The present invention provides a cell culture medium formulation that supports the in vitro cultivation, particularly in suspension, of mammalian cells, particularly epithelial cells and fibroblast cells, and methods for cultivating mammalian cells in suspension in vitro using these media. The media comprise a basal medium and a polyanionic or polyanionic compound, preferably a polysulfonated or polysulfated compound, and more preferably dextran sulfate. The present invention also provides chemically defined, protein-free eukaryotic cell culture media comprising an iron chelate and zinc, which is capable of supporting the growth (and particularly the high-density growth of mammalian cells) in suspension culture, increasing the level of expression of recombinant protein in cultured cells, and/or increasing virus production in cultured cells.
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4.发明授权公开(公告)号：US06627426B2
公开(公告)日：2003-09-30
申请号：US09576900
申请日：2000-05-23
IPC分类号： C12N100
CPC分类号： A61L2/0094 , A61L2/20 , F26B3/12
摘要： The present invention relates generally to a method to reduce, substantially reduce, inactivate or eliminate adventitious agents and/or toxins in a sample, particuarly in nutritive media, media supplements, media subgroups and buffer formulations. Specifically, the present invention provides powdered nutritive media, media supplements and media subgroups produced by the methods of the invention, particuarly cell culture media supplements (including powdered sera such as powdered fetal bovine serum (FBS)). The invention further provides powdered buffer formulations produced by the methods of the invention. The invention also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these nutritive media, media supplements, media subgroups and buffer formulations. The invention also relates to methods for producing storage stable cells having reduced, substantially reduced or eliminated adventitious agents or toxins.
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5.发明授权Method for enzymatic regeneration of cell culture media and media kits therefor 失效用于细胞培养基和媒体载体的酶促再生的方法
公开(公告)号：US5106747A
公开(公告)日：1992-04-21
申请号：US593930
申请日：1990-10-05
申请人： Richard M. Fike
发明人： Richard M. Fike
CPC分类号： C12N1/38
摘要： A multienzyme system capable of reducing waste products and synthesizing nutrients in a cell culture system is disclosed. In one embodiment, lactic dehydrogenase and leucine dehydrogenase are employed in conjunction with amino acid precursors and a recycling co-factor to achieve the synthesis of amino acids and a reduction in the levels of ammonia and lactic acid. The multienzyme system of the invention may be added directly to the cell culture system or it may be microencapsulated. Alternatively, the invention may be utilized in conjunction with a container unit in which cell culture medium contacts the multienzyme system external to the main culture vessel compartment.
摘要翻译： 公开了能够减少细胞培养系统中的废物和合成营养物质的多酶体系。 在一个实施方案中，乳酸脱氢酶和亮氨酸脱氢酶与氨基酸前体和循环辅因子结合使用以实现氨基酸的合成和氨和乳酸的降低。 本发明的多酶体系可以直接添加到细胞培养体系中，也可以是微胶囊化的。 或者，本发明可以与细胞培养基与主培养容器隔室外部的多酶系统接触的容器单元结合使用。
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公开(公告)号：US20080261308A1
公开(公告)日：2008-10-23
申请号：US12018035
申请日：2008-01-22
申请人： Richard M. Fike , William Whitford , Richard F. Hassett , Jeffrey W. Biddle , Laurel Biddle , Christine M. Biddle , Robert C. Radominski , Barbara M. Dadey
发明人： Richard M. Fike , William Whitford , Richard F. Hassett , Jeffrey W. Biddle , Laurel Biddle , Christine M. Biddle , Robert C. Radominski , Barbara M. Dadey
IPC分类号： C12N5/00
CPC分类号： C12N5/0018 , C12N1/00 , C12N2500/36 , C12N2500/60
摘要： The present invention relates to nutritive medium, medium supplement, media subgroup and buffer formulations. The present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, e.g., cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention provides methods for production of media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations.
摘要翻译： 本发明涉及营养培养基，培养基，培养基和缓冲剂制剂。 本发明提供粉末营养培养基，培养基和中等亚组分制剂，例如细胞培养基补充剂（包括粉末状血清如粉状胎牛血清（FBS）），培养基亚组制剂和包含所有必需营养因子的细胞培养基 促进细胞的体外培养。 本发明进一步提供粉末缓冲剂制剂，其在用溶剂重构时产生特定的离子和pH条件。 本发明提供了制备培养基，培养基，培养基和缓冲液制剂的方法，还提供用于培养原核和真核细胞，特别是细菌细胞，酵母细胞，植物细胞和动物细胞（包括人细胞）的试剂盒和方法，其使用 这些干粉营养介质，培养基补充剂，培养基和缓冲液配方。
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公开(公告)号：US20130109094A1
公开(公告)日：2013-05-02
申请号：US13614588
申请日：2012-09-13
申请人： Richard M. Fike , William Whitford , Richard F. Hassett , Jeffrey W. Biddle , Laurel Biddle , Christine M. Biddle , Robert C. Radominski , Barbara M. Dadey
发明人： Richard M. Fike , William Whitford , Richard F. Hassett , Jeffrey W. Biddle , Laurel Biddle , Christine M. Biddle , Robert C. Radominski , Barbara M. Dadey
IPC分类号： C12N5/00
CPC分类号： C12N5/0018 , C12N1/00 , C12N2500/36 , C12N2500/60
摘要： The present invention relates to nutritive medium, medium supplement, media subgroup and buffer formulations. The present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, e.g., cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention provides methods for production of media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations.
摘要翻译： 本发明涉及营养培养基，培养基，培养基和缓冲剂制剂。 本发明提供粉末营养培养基，培养基和中等亚组分制剂，例如细胞培养基补充剂（包括粉末状血清如粉状胎牛血清（FBS）），培养基亚组制剂和包含所有必需营养因子的细胞培养基 促进细胞的体外培养。 本发明进一步提供粉末缓冲剂制剂，其在用溶剂重构时产生特定的离子和pH条件。 本发明提供了制备培养基，培养基，培养基和缓冲液制剂的方法，还提供用于培养原核和真核细胞，特别是细菌细胞，酵母细胞，植物细胞和动物细胞（包括人细胞）的试剂盒和方法，其使用 这些干粉营养介质，培养基补充剂，培养基和缓冲液配方。
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公开(公告)号：US20080311660A1
公开(公告)日：2008-12-18
申请号：US11969338
申请日：2008-01-04
申请人： Richard M. Fike , William Whitford , Richard F. Hassett , Jeffrey W. Biddle , Laurel Biddle , Christine M. Biddle , Robert C. Radominski , Barbara M. Dadey
发明人： Richard M. Fike , William Whitford , Richard F. Hassett , Jeffrey W. Biddle , Laurel Biddle , Christine M. Biddle , Robert C. Radominski , Barbara M. Dadey
IPC分类号： C12N5/06
CPC分类号： C12N5/0018 , C12N1/00 , C12N2500/36 , C12N2500/60
摘要： The present invention relates to nutritive medium, medium supplement, media subgroup and buffer formulations. The present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, e.g., cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention provides methods for production of media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations.
摘要翻译： 本发明涉及营养培养基，培养基，培养基和缓冲剂制剂。 本发明提供粉末营养培养基，培养基和中等亚组分制剂，例如细胞培养基补充剂（包括粉末状血清如粉状胎牛血清（FBS）），培养基亚组制剂和包含所有必需营养因子的细胞培养基 促进细胞的体外培养。 本发明进一步提供粉末缓冲剂制剂，其在用溶剂重构时产生特定的离子和pH条件。 本发明提供了制备培养基，培养基，培养基和缓冲液制剂的方法，还提供用于培养原核和真核细胞，特别是细菌细胞，酵母细胞，植物细胞和动物细胞（包括人细胞）的试剂盒和方法，其使用 这些干粉营养介质，培养基补充剂，培养基和缓冲液配方。
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