公开(公告)号：US5837446A

公开(公告)日：1998-11-17

申请号：US482517

申请日：1995-06-07

申请人： Stephen N. Cozzette ,  Graham Davis ,  Jeanne Itak ,  Imants R. Lauks ,  Sylvia Piznik ,  Nicolaas Smit ,  Susan Steiner ,  Paul Van Der Werf ,  Henry J. Wieck ,  Randall M. Mier, deceased

发明人： Stephen N. Cozzette ,  Graham Davis ,  Jeanne Itak ,  Imants R. Lauks ,  Sylvia Piznik ,  Nicolaas Smit ,  Susan Steiner ,  Paul Van Der Werf ,  Henry J. Wieck ,  Randall M. Mier, deceased

IPC分类号： H04R1/44 ,  B01L3/00 ,  B01L3/02 ,  C12Q1/00 ,  G01N1/28 ,  G01N27/07 ,  G01N27/327 ,  G01N27/333 ,  G01N27/414 ,  G01N27/416 ,  G01N33/543 ,  G01N35/00 ,  G01N35/10 ,  G01V9/00 ,  G10K11/00 ,  H04R20060101 ,  H04R1/02 ,  H04R15/00 ,  G01N33/53 ,  G01N33/68

CPC分类号： B01L3/0268 ,  B01L3/5027 ,  C12Q1/001 ,  G01N33/5438 ,  G01N35/0099 ,  G01V9/00 ,  G10K11/006 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0877 ,  B01L3/5088 ,  G01N2035/00059 ,  G01N2035/00237 ,  G01N2035/00455 ,  G01N2035/00534 ,  G01N2035/1034 ,  G01N2035/1076 ,  G01N35/109 ,  Y10S435/817 ,  Y10S436/806

摘要： An efficient method for the microfabrication of electronic devices which have been adapted for the analyses of biologically significant analyte species is described. The techniques of the present invention allow for close control over he dimensional features of the various components and layers established on a suitable substrate. Such control extends to those parts of the devices which incorporate the biological components which enable these devices to function as biological sensors. The materials and methods disclosed herein thus provide an effective means for the mass production of uniform wholly microfabricated biosensors. Various embodiments of the devices themselves are described herein which are especially suited for real time analyses of biological samples in a clinical setting. In particular, the present invention describes assays which can be performed using certain ligand/ligand receptor-based biosensor embodiments. The present invention also discloses a novel method for the electrochemical detection of particular analyte species of biological and physiological significance using an substrate/label signal generating pair which produces a change in the concentration of electroactive species selected from the group consisting of dioxygen and hydrogen peroxide.

摘要翻译： 描述了适用于分析生物学重要的分析物种的电子装置的微细加工的有效方法。 本发明的技术允许对其在合适的衬底上建立的各种组件和层的尺寸特征进行密切的控制。 这种控制延伸到结合生物组分的装置的那些部分，使得这些装置能够用作生物传感器。 因此，本文公开的材料和方法提供了用于批量生产均匀的全微生物传感器的有效手段。 本文描述了装置本身的各种实施例，其特别适用于临床环境中的生物样品的实时分析。 特别地，本发明描述了可以使用某些基于配体/配体受体的生物传感器实施方案进行的测定。 本发明还公开了一种利用基板/标记信号产生对电化学检测特定分析物种的生物学和生理意义的新方法，其产生选自二氧和过氧化氢的电活性物质浓度的变化。

公开(公告)号：US5837454A

公开(公告)日：1998-11-17

申请号：US484095

申请日：1995-06-07

申请人： Stephen N. Cozzette ,  Graham Davis ,  Imants R. Lauks ,  Randall M. Mier, deceased ,  Sylvia Piznik ,  Nicolaas Smit ,  Paul Van Der Werf ,  Henry J. Wieck ,  Susan Steiner ,  Jeanne Itak

发明人： Stephen N. Cozzette ,  Graham Davis ,  Imants R. Lauks ,  Randall M. Mier, deceased ,  Sylvia Piznik ,  Nicolaas Smit ,  Paul Van Der Werf ,  Henry J. Wieck ,  Susan Steiner ,  Jeanne Itak

IPC分类号： H04R1/44 ,  B01L3/00 ,  B01L3/02 ,  C12Q1/00 ,  G01N1/28 ,  G01N27/07 ,  G01N27/327 ,  G01N27/333 ,  G01N27/414 ,  G01N27/416 ,  G01N33/543 ,  G01N35/00 ,  G01N35/10 ,  G01V9/00 ,  G10K11/00 ,  H04R20060101 ,  H04R1/02 ,  H04R15/00 ,  G01N33/545

CPC分类号： B01L3/0268 ,  B01L3/5027 ,  C12Q1/001 ,  G01N33/5438 ,  G01N35/0099 ,  G01V9/00 ,  G10K11/006 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0877 ,  B01L3/5088 ,  G01N2035/00059 ,  G01N2035/00237 ,  G01N2035/00455 ,  G01N2035/00534 ,  G01N2035/1034 ,  G01N2035/1076 ,  G01N35/109 ,  Y10S435/817 ,  Y10S436/806

摘要： An efficient method for the microfabrication of electronic devices which have been adapted for the analyses of biologically significant analyte species is described. The techniques of the present invention allow for close control over the dimensional features of the various components and layers established on a suitable substrate. Such control extends to those parts of the devices which incorporate the biological components which enable these devices to function as biological sensors. The materials and methods disclosed herein thus provide an effective means for the mass production of uniform wholly microfabricated biosensors. Various embodiments of the devices themselves are described herein which are especially suited for real time analyses of biological samples in a clinical setting. In particular, the present invention describes assays which can be performed using certain ligand/ligand receptor-based biosensor embodiments. The present invention also discloses a novel method for the electrochemical detection of particular analyte species of biological and physiological significance using an substrate/label signal generating pair which produces a change in the concentration of electroactive species selected from the group consisting of dioxygen and hydrogen peroxide.