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    Probe composition and method
    4.
    发明申请
    Probe composition and method 失效
    探头组成和方法

    公开(公告)号：US20050112609A1

    公开(公告)日：2005-05-26

    申请号：US10825624

    申请日：2004-04-14

    Applicant: Paul Grossman ,  Steven Fung ,  Steven Menchen ,  Sam Woo ,  Emily Winn-Deen

    Inventor： Paul Grossman ,  Steven Fung ,  Steven Menchen ,  Sam Woo ,  Emily Winn-Deen

    IPC: C12Q1/68

    CPC classification number: C12Q1/6816 ,  C12Q1/6825 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q2600/156 ,  G01N27/447 ,  Y10T436/143333 ,  C12Q2525/197 ,  C12Q2525/203 ,  C12Q2537/143 ,  C12Q2565/131 ,  C12Q2565/125 ,  C12Q2565/107 ,  C12Q2521/501 ,  C12Q2563/155 ,  C12Q2563/119 ,  C12Q2549/119

    Abstract: Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In the method, a mixture of sequence-specific probes are reacted with the target polynucleotide under hybridization conditions, and the hybridized probes are treated to selectively modify those probes which are bound to the target polynucleotide in a base-specific manner. The resulting labeled probes include a polymer chain which imparts to each different-sequence probe, a distinctive ratio of charge/translational frictional drag, and a detectable label. The labeled probes are fractionated by electrophoresis in a non-sieving matrix, and the presence of one or more selected sequences in the target polynucleotide are detected according to the observed electrophoretic migration rates of the labeled probes in a non-sieving medium.

    Abstract translation: 用于检测靶多核苷酸中的一个或多个选择的多核苷酸区域的方法和组合物。 在该方法中，将序列特异性探针的混合物与靶多核苷酸在杂交条件下反应，并对杂交的探针进行处理以选择性地修饰以碱基特异性方式结合靶多核苷酸的那些探针。 所得到的标记探针包括赋予每个不同序列探针的聚合物链，电荷/平移摩擦阻力的独特比例和可检测标记。 标记的探针通过在非筛选基质中的电泳分级分离，并且根据在非筛选培养基中标记的探针的观察到的电泳迁移速率来检测靶多核苷酸中一个或多个选定序列的存在。

    Methods and kits for methylation detection
    6.
    发明申请
    Methods and kits for methylation detection 有权
    用于甲基化检测的方法和试剂盒

    公开(公告)号：US20050266458A1

    公开(公告)日：2005-12-01

    申请号：US11119985

    申请日：2005-05-02

    Applicant: Mark Andersen ,  Jer-Kang Chen ,  Michael Hunkapiller ,  Steven Menchen

    Inventor： Mark Andersen ,  Jer-Kang Chen ,  Michael Hunkapiller ,  Steven Menchen

    IPC: C12Q1/68

    CPC classification number: C12Q1/68 ,  C12Q1/6827 ,  C12Q1/6862 ,  C12Q2537/164 ,  C12Q2521/501

    Abstract: Ligation-based methods and kits are disclosed for determining the degree of methylation of one or more target nucleotides. In certain embodiments, the methylation status of one or more target nucleotides is determined by generating misligation products. In certain embodiments, at least one target nucleotide is amplified prior to the ligation reaction. In certain embodiments, at least one ligation product, at least one ligation product surrogate, at least one misligation product, at least one misligation product surrogate, or combinations thereof are amplified. In certain embodiments, one or more ligation probes comprise at least one nucleotide analog, at least one Modification, at least one mismatched nucleotide, or combinations thereof.

    Abstract translation: 公开了基于连接的方法和试剂盒以确定一个或多个靶核苷酸的甲基化程度。 在某些实施方案中，通过产生错误产物来确定一个或多个靶核苷酸的甲基化状态。 在某些实施方案中，在连接反应之前扩增至少一种靶核苷酸。 在某些实施方案中，扩增至少一种连接产物，至少一种连接产物替代物，至少一种错误产物，至少一种错误产物替代物或其组合。 在某些实施方案中，一个或多个连接探针包含至少一个核苷酸类似物，至少一个修饰，至少一个错配核苷酸或其组合。

    Methods of Labelling Polynucleotides with Dibenzorhodamine Dyes
    8.
    发明申请
    Methods of Labelling Polynucleotides with Dibenzorhodamine Dyes 审中-公开
    用二苄唑胺染料标记多核苷酸的方法

    公开(公告)号：US20070099210A1

    公开(公告)日：2007-05-03

    申请号：US11466085

    申请日：2006-08-21

    Applicant: Scott Benson ,  Joe Lam ,  Steven Menchen

    Inventor： Scott Benson ,  Joe Lam ,  Steven Menchen

    IPC: C12Q1/68 ,  C07H21/04 ,  C07D491/22

    CPC classification number: C09B11/24 ,  C07D209/60 ,  C07D221/10 ,  C07D311/78 ,  C07D491/14 ,  C07D491/147 ,  C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C07H21/04 ,  C09B11/02 ,  C12Q1/6869 ,  Y10T436/143333 ,  C12Q2563/107 ,  C12Q2527/125

    Abstract: Dibenzorhodamine compounds having the structure are disclosed, including nitrogen- and aryl-substituted forms thereof. In addition, two intermediates useful for synthesizing such compounds are disclosed, a first intermediate having the structure including nitrogen- and aryl-substituted forms thereof, and a second intermediate having the structure including nitrogen- and aryl-substituted forms thereof, wherein substituents at positions C14 to C18 taken separately are selected from the group consisting of hydrogen, chlorine, fluorine, lower alkyl, carboxylic acid, sulfonic acid, —CH2OH, alkoxy, phenoxy, linking group, and substituted forms thereof. The invention further includes energy transfer dyes comprising the dibenzorhodamine compounds, nucleosides labeled with the dibenzorhodamine compounds, and nucleic acid analysis methods employing the dibenzorhodamine compounds.

    Abstract translation: 公开了具有该结构的二苄氧苯胺类化合物，包括其氮和芳基取代的形式。 此外，公开了可用于合成这些化合物的两种中间体，具有包括其氮和芳基取代形式的结构的第一中间体和具有其氮和芳基取代形式的结构的第二中间体，其中位置 C14至C18分别选自氢，氯，氟，低级烷基，羧酸，磺酸，-CH 2 OH，烷氧基，苯氧基，连接基团及其取代形式 。 本发明还包括包含二苄基罗丹胺化合物的能量转移染料，用二苯并呋喃胺化合物标记的核苷，以及使用二苯并呋喃胺化合物的核酸分析方法。

    Multi-chromophoric quencher constructs for use in high sensitivity energy transfer probes
    10.
    发明申请
    Multi-chromophoric quencher constructs for use in high sensitivity energy transfer probes 有权

    公开(公告)号：US20060063186A1

    公开(公告)日：2006-03-23

    申请号：US11226069

    申请日：2005-09-14

    Applicant: Scott Benson ,  Steven Menchen ,  Krishna Upadhya

    Inventor： Scott Benson ,  Steven Menchen ,  Krishna Upadhya

    IPC: C12Q1/68 ,  C07H21/00 ,  C07H21/04

    CPC classification number: C07C245/08 ,  C07C50/18 ,  C07D311/90 ,  C07H21/00 ,  C07H21/04 ,  C09B6/00 ,  C09B11/08 ,  C09B33/02 ,  C09B35/021 ,  C09B35/50 ,  G01N33/542

    Abstract: Dark quencher constructs, termed “multi-chromophoric quenchers” are described herein that comprise at least two dark quenching moieties, which can be the same or different, linked together by at least one multivalent linking moiety. The structure of the multi-chromophoric quenchers can be varied to selectively enhance quenching within a specific range of reporter emission wavelengths. This can be accomplished by linking together, into a single molecule, two or more identical quenchers, by reacting the quenchers with a multivalent linker. The structure of the multi-chromophoric quencher can also be varied to quench a broader range of reporter emission wavelengths than previously possible. This can be accomplished by linking together, into a single molecule, two or more different quenchers, by reacting the quenchers with a multivalent linker. The structure of the multi-chromophoric quencher can also be varied to simultaneously broaden the absorption range and increase the total absorption within the absorption range. This can be done by combining the two concepts described above. In other words, multiple types of quenching moieties can be employed to increase the absorption range and a multiple number of each type of quenching moiety can be used to increase the total absorptivity within the absorption range. The multi-chromophoric quenchers can be tethered to probes for biomolecules, insoluble supports and/or fluorescent dyes for use in a wide variety of biomolecular assays.

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