利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

13 条记录 (耗时 0.023 秒)

    Methods of Labelling Polynucleotides with Dibenzorhodamine Dyes
    1.
    发明申请
    Methods of Labelling Polynucleotides with Dibenzorhodamine Dyes 审中-公开
    用二苄唑胺染料标记多核苷酸的方法

    公开(公告)号：US20070099210A1

    公开(公告)日：2007-05-03

    申请号：US11466085

    申请日：2006-08-21

    申请人： Scott Benson ,  Joe Lam ,  Steven Menchen

    发明人： Scott Benson ,  Joe Lam ,  Steven Menchen

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C07D491/22

    CPC分类号： C09B11/24 ,  C07D209/60 ,  C07D221/10 ,  C07D311/78 ,  C07D491/14 ,  C07D491/147 ,  C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C07H21/04 ,  C09B11/02 ,  C12Q1/6869 ,  Y10T436/143333 ,  C12Q2563/107 ,  C12Q2527/125

    摘要： Dibenzorhodamine compounds having the structure are disclosed, including nitrogen- and aryl-substituted forms thereof. In addition, two intermediates useful for synthesizing such compounds are disclosed, a first intermediate having the structure including nitrogen- and aryl-substituted forms thereof, and a second intermediate having the structure including nitrogen- and aryl-substituted forms thereof, wherein substituents at positions C14 to C18 taken separately are selected from the group consisting of hydrogen, chlorine, fluorine, lower alkyl, carboxylic acid, sulfonic acid, —CH2OH, alkoxy, phenoxy, linking group, and substituted forms thereof. The invention further includes energy transfer dyes comprising the dibenzorhodamine compounds, nucleosides labeled with the dibenzorhodamine compounds, and nucleic acid analysis methods employing the dibenzorhodamine compounds.

    摘要翻译： 公开了具有该结构的二苄氧苯胺类化合物，包括其氮和芳基取代的形式。 此外，公开了可用于合成这些化合物的两种中间体，具有包括其氮和芳基取代形式的结构的第一中间体和具有其氮和芳基取代形式的结构的第二中间体，其中位置 C14至C18分别选自氢，氯，氟，低级烷基，羧酸，磺酸，-CH 2 OH，烷氧基，苯氧基，连接基团及其取代形式 。 本发明还包括包含二苄基罗丹胺化合物的能量转移染料，用二苯并呋喃胺化合物标记的核苷，以及使用二苯并呋喃胺化合物的核酸分析方法。

    Multi-chromophoric quencher constructs for use in high sensitivity energy transfer probes
    3.
    发明申请
    Multi-chromophoric quencher constructs for use in high sensitivity energy transfer probes 有权

    公开(公告)号：US20060063186A1

    公开(公告)日：2006-03-23

    申请号：US11226069

    申请日：2005-09-14

    申请人： Scott Benson ,  Steven Menchen ,  Krishna Upadhya

    发明人： Scott Benson ,  Steven Menchen ,  Krishna Upadhya

    IPC分类号： C12Q1/68 ,  C07H21/00 ,  C07H21/04

    CPC分类号： C07C245/08 ,  C07C50/18 ,  C07D311/90 ,  C07H21/00 ,  C07H21/04 ,  C09B6/00 ,  C09B11/08 ,  C09B33/02 ,  C09B35/021 ,  C09B35/50 ,  G01N33/542

    摘要： Dark quencher constructs, termed “multi-chromophoric quenchers” are described herein that comprise at least two dark quenching moieties, which can be the same or different, linked together by at least one multivalent linking moiety. The structure of the multi-chromophoric quenchers can be varied to selectively enhance quenching within a specific range of reporter emission wavelengths. This can be accomplished by linking together, into a single molecule, two or more identical quenchers, by reacting the quenchers with a multivalent linker. The structure of the multi-chromophoric quencher can also be varied to quench a broader range of reporter emission wavelengths than previously possible. This can be accomplished by linking together, into a single molecule, two or more different quenchers, by reacting the quenchers with a multivalent linker. The structure of the multi-chromophoric quencher can also be varied to simultaneously broaden the absorption range and increase the total absorption within the absorption range. This can be done by combining the two concepts described above. In other words, multiple types of quenching moieties can be employed to increase the absorption range and a multiple number of each type of quenching moiety can be used to increase the total absorptivity within the absorption range. The multi-chromophoric quenchers can be tethered to probes for biomolecules, insoluble supports and/or fluorescent dyes for use in a wide variety of biomolecular assays.

    Mobility-modifying cyanine dyes
    4.
    发明申请
    Mobility-modifying cyanine dyes 审中-公开
    迁移性改性花青染料

    公开(公告)号：US20050107617A1

    公开(公告)日：2005-05-19

    申请号：US10801092

    申请日：2004-03-15

    申请人： Steven Menchen ,  Scott Benson ,  Barnett Rosenblum ,  Shaheer Khan

    发明人： Steven Menchen ,  Scott Benson ,  Barnett Rosenblum ,  Shaheer Khan

    IPC分类号： C09B23/00 ,  C07F9/24 ,  C07H21/00 ,  C09B1/00 ,  C09B11/28 ,  C09B23/02 ,  G01N27/447 ,  G01N33/52 ,  G01N33/533 ,  C07D417/02 ,  C07D413/02 ,  C07D43/02

    CPC分类号： C07H21/00 ,  C07F9/2408 ,  C09B23/02 ,  G01N27/44726

    摘要： The present invention provides a novel class of fluorescent cyanine dye compounds that are modified at one of the hetercyclic ring nitrogen atoms with a mobility-modifying moiety that permits the electrophoretic mobilities of polynucleotides labeled with the mobility-modifying cyanine dyes to be adjusted or tuned in a predictable fashion while retaining enzymatic activity. The ability to predictably tune the relative electrophoretic mobilities of the dyes permits the creation of sets of mobility-matched fluorescent dyes of a variety of structures for a variety of applications, including fluorescence-based 4-color nucleic acid sequencing reactions.

    摘要翻译： 本发明提供了一类新颖的荧光花青染料化合物，其在一个杂环氮原子上被修饰，具有迁移率调节部分，其允许调节或调谐用迁移率调节花青染料标记的多核苷酸的电泳迁移率 一种可预测的方式，同时保留酶活性。 可预测地调节染料的相对电泳迁移率的能力允许为各种应用（包括基于荧光的4色核酸测序反应）创建各种结构的迁移率匹配荧光染料组。

    4,7-dichlororhodamine dyes labeled polynucleotides
    6.
    发明申请
    4,7-dichlororhodamine dyes labeled polynucleotides 失效
    4,7-二氯罗丹明染料标记的多核苷酸

    公开(公告)号：US20050112781A1

    公开(公告)日：2005-05-26

    申请号：US10788660

    申请日：2004-02-26

    申请人： Linda Lee ,  Scott Benson ,  Barnett Rosenblum ,  Sandra Spurgeon ,  Ronald Graham

    发明人： Linda Lee ,  Scott Benson ,  Barnett Rosenblum ,  Sandra Spurgeon ,  Ronald Graham

    IPC分类号： C07D311/88 ,  C07D401/06 ,  C07D401/14 ,  C07D405/14 ,  C07D491/14 ,  C07D491/147 ,  C12Q1/68 ,  G01N33/543

    CPC分类号： C07D401/06 ,  C07D401/14 ,  C07D405/14 ,  C07D491/147 ,  C12Q1/6869 ,  C12Q2563/107 ,  C12Q2535/101

    摘要： A set of 4,7-dichlororhodamine compounds useful as fluorescent dyes are disclosed having the structures wherein R1-R6 are hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group, or, when taken together, R1 and R6 is benzo, or, when taken together, R4 and R5 is benzo; R7-R10, R12-R16 and R18 may be hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group; R11 and R17 may be hydrogen, lower alkyl, lower alkene, lower alkyne, phenyl, aryl, linking group; Y1-Y4 are hydrogen, lower alkyl, or cycloalkyl, or, when taken together, Y1 and R2, Y2 and R1 Y3 and R3, and/or Y4 and R4 is propano, ethano, or substituted forms thereof, and X1-X3 taken separately are hydrogen, chlorine, fluorine, lower alkyl, carboxylate, sulfonate, hydroxymethyl, and linking group, or any combinations thereof. In another aspect, the invention includes reagents labeled with the 4,7-dichlororhodamine dye compounds, including deoxynucleotides, dideoxynucleotides, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.

    摘要翻译： 公开了一组用作荧光染料的4,7-二氯罗丹明化合物，其中R 1 -R 6是氢，氟，氯，低级烷基，低级烯烃 ，低级炔烃，磺酸盐，砜，氨基，酰氨基，腈，低级烷氧基，连接基团，或者当R 1和R 6一起为苯并或 当R 4和R 5一起时，是苯并; R 7 -R 10，R 12 -R 16和R 18可以是 氢，氟，氯，低级烷基，低级烯，低级炔，磺酸，砜，氨基，酰氨基，腈，低级烷氧基，连接基团; R 11和R 17可以是氢，低级烷基，低级烯，低级炔，苯基，芳基，连接基团; Y 1 -Y 4是氢，低级烷基或环烷基，或者当一起为Y 1和R 2时， Y 1，Y 2和R 3，Y 3和R 3，和/或Y 3， R 4和R 4是丙酰基，乙酸或其取代形式，分别取代的X 1 -X 3 - 3是氢， 氯，氟，低级烷基，羧酸酯，磺酸酯，羟甲基和连接基团，或其任何组合。 另一方面，本发明包括用4,7-二氯罗丹明染料化合物标记的试剂，包括脱氧核苷酸，双脱氧核苷酸和多核苷酸。 在另一方面，本发明包括利用这种染料化合物的方法和包括双脱氧多核苷酸测序和片段分析方法的试剂。

    Treatment of metal oxide surfaces
    9.
    发明申请
    Treatment of metal oxide surfaces 审中-公开
    处理金属氧化物表面

    公开(公告)号：US20110206578A1

    公开(公告)日：2011-08-25

    申请号：US13033206

    申请日：2011-02-23

    申请人： Evan Foster ,  Jessica Reed ,  Heather Shepherd ,  Christina Inman ,  Scott Benson

    发明人： Evan Foster ,  Jessica Reed ,  Heather Shepherd ,  Christina Inman ,  Scott Benson

    IPC分类号： B01L9/00 ,  B05D3/10 ,  B05D3/06 ,  B05D7/22 ,  B05D3/00

    CPC分类号： C12Q1/6834 ,  B01J19/0046 ,  B01J2219/00612 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00648 ,  B01J2219/00722 ,  B01L3/502707

    摘要： Methods for activating and/or reactivating a metal oxide surface for deposition, immobilizing, and/or growing a biological sample (e.g., an oligonucleotide, a polynucleotide, functionalized particle, a polymer, etc.) thereon are disclosed. The metal oxide surface (e.g., zirconium oxide) can be activated and/or reactivated by exposing the metal oxide surface to various activation and/or treatment protocols capable of enhancing a slide's activity relative to the activity of an untreated slide. The activation and/or treatment protocol can include subjecting the slide (or at least the metal oxide layer) to an oxygen plasma treatment and/or a peroxide solution for an amount of time sufficient to activate and/or reactivate the metal oxide surface. Activated sequencing slides capable of incorporation, for example, into various embodiments of a flow cell for use in NGS platforms are also disclosed herein.

    摘要翻译： 公开了用于活化和/或重新激活用于在其上沉积，固定和/或生长生物样品（例如寡核苷酸，多核苷酸，官能化颗粒，聚合物等）的金属氧化物表面的方法。 金属氧化物表面（例如，氧化锆）可以通过将金属氧化物表面暴露于能够相对于未处理载玻片的活性增强载玻片的活性的各种活化和/或处理方案而被活化和/或再活化。 活化和/或处理方案可以包括使载玻片（或至少金属氧化物层）经历氧等离子体处理和/或过氧化物溶液足以活化和/或重新活化金属氧化物表面的时间量。 本文还公开了能够并入例如用于NGS平台的流动池的各种实施方案中的活化测序载玻片。

    Determining advertising statistics for advertisers and/or advertising networks
    10.
    发明申请
    Determining advertising statistics for advertisers and/or advertising networks 审中-公开
    确定广告客户和/或广告网络的广告统计信息

    公开(公告)号：US20070239532A1

    公开(公告)日：2007-10-11

    申请号：US11394194

    申请日：2006-03-31

    申请人： Scott Benson ,  Gokul Rajaram

    发明人： Scott Benson ,  Gokul Rajaram

    IPC分类号： G06Q30/00 ,  G06F15/173

    CPC分类号： G06Q30/02 ,  G06Q30/0246 ,  G06Q30/0277

    摘要： A system determines a number of times that clients access a group of documents associated with a web site or a group of web sites. At least one document in the group of documents includes at least one advertisement. The system further determines a percentage of client views for an advertiser or an advertising network across the web site or group of web sites based on the determined number of times.

    摘要翻译： 系统确定客户端访问与网站或一组网站相关联的一组文档的次数。 文档组中的至少一个文档包括至少一个广告。 该系统还基于所确定的次数来确定跨网站或网站组的广告商或广告网络的客户端视图的百分比。